Rttn (NM_001170436) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217755 representing NM_001170436 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTGGCGGGGCTCATCAGGAAGCTCGGCCATCAGCTGACAGAGATCAGGGAACGCGCCCTCAAAA GTATCCTGTGCAAGATCGAGCACAACTTGATCTGCTGTGCTGACTTGATTCAGGAAAGGTTGCTTTTTCT CCATTTGCTTGAATGGTTCAATTTCCCGTCCGTCCCAATGAAAGAGGAGGTTCTGAACTTGTTAAGCAGA TTGGTTAAGTATCCTCCAGCTGTCCAACACTTGATTGACCTTGGTGCAGTAGAGTTCTTATCTAAGCTCC GTCCAAATGTGGAGCCAGCAATTCAGGCTGAGATTGATGGCATTCTCGATGGACTTTTCATTCTTCCTTC AGAAGTTCCTGCACTGCGTTCTGCCACTTACCAACCTGATCAAAATGAATTGTCACAACAACCTGAAGTT TTAACAGGCTATTTTCCCCAAGACAAAAGTAATCTCCAGCAGATGGAAGTCCCACCAGGGCCAGTGGGAA ACCCAACTGTGAAGTGGCTGAGGTTCTCTGTGTTTCCTTGGCTACCCCTGACCACAACAGACAGACATGT TCTCTCCTCCAATGAAAGCTCCTTGAGAAGTAGTAACCACACTCTGATCTGGAACACCTGTGAGCTTCTG AAGGACGTGATCATGCAAGACTTTCCTGCGGAGATTTTTCTTCAGAGGCCTAAGATTGTTCAGGGCCTTT TGTGTCTGGTGAAGCTGGCCTTTGGAGGAGATGGGAAGCATCGTCTCGCCTTACAGTCAGTGTCCTGTTT GCAGCAGCTGTGCACAAGCTTAAGAAACAGACTCAACTTTTACAGAGATCCCAACCTTTTCTCGAATAAA CAAGACACAATCTCCCAGAATTCTTCTTTGTCTTACTGTCATGAAGCAAGAGGTCCTTACCATTCCCCAA ACCCGTCCCCAGGAAGCAGTAGCTCTCGGCCTTCCGTGGTTGGGCGTACAGGCCAGCGACCCCGAGGAGA TGGCCAGGACTGGGATGCAGTGTCTTCCAGCGGGAGCAGCAGTCACGCACATGTGAACTCTAGGCTGTCT GTGCGTTCTCCCTTGGATGTGGCGCATGCAGATCTGCCGGAGCTGGAAACAGAGGACGCATTGGAGCTGC ACTTCCAGCAGCTTAGTCTTCCTCAGTTCTGCATCTCCACCCTGGACGCAGCAGTCCCTCTGCTGAGGAC AGGTAGCAGAGAAATGGTAATAAGAGTTTTGGAACTGCTTGGAGAGGACCTGTTGCTCATTGGACAAGCA ATTTCATCTGAAATCTGGGATGATAACAGCCTTTTTGCTATAGATGTGAAAGAGAAACTGCTCCAAGTGT TGGGGTCCCTTGGAGAAACTATGTGCTACCGAAAGACCAGTTTTAGCTTGGAGCAAGCAGAGGCTATACT AGTGCACCACAGGATGGCCTTTGTCAGCATCTCACTGTTCACCGTTCGACTGTTACAAATTCTTCTCCCT GTTGAAAAGGCTAGTGAGTTTTTGCCAGAGTCCATGTCAGCAGCATTATTTTTTGTCTCGTTGGACATGC CTATTTCTTTGGAATATCCAGAGATTCATGACACTGTTGTGGCTTATTTGGAACAGCTGGATTCTGAAAA TTACAGCATTTATAAGCGAACTGCAGAGGCTGTTTATTCAATTGAATGTACCTGTAACTTCCTATCTGAT GTGGGGAAAGAGGGAGAAAAGAATCTCCTAGAATTAGTGGAGCTGGCAGACCAAGCCTTGCGTAGCTTTT CCTATCATCAGCATTCCCCCCTCATAAAGGAAGTCATCTGCCTTTGTTCAAAGATCTGGAAATCTGCACA GGCTAGTCCACTACTCCAGGGAGAGAGCCAGAAGGTGTTTCTCCATATGCTGTCTCACCCATTGCCACAG GTGAAGGTGGAGACTTACCAGTGCTGCCTGGAGATTGTGAAGGAGTGTTTAGGTGTCCATAATGTCACTA AACCTGTGTCTTCACTGTGTAGTGGAATTCATTTTCTGCTTCATCCAAAAGTTCTCTATGAAATCTCTGC ATTTGGTATTCAAGAATCCAAAAATGAGGTGAATGCTGCTGCCAAGGCTGTTCTCCTGTATCTGCTTCAA GGACGATTTATGATGACAGCTCTGACCTGGAACAAGTTTATCGAGTCTCTCTGTCCTGTCATTCCAGTGC TACAGGGCTATGCTGATACAGAAGACCCTTTAGGCAACTGCATTCTCCTTCTAAGCAAAGTGAGTTCTGA GACAGGAGAAGGCATTCTCCCCTGCACTACCCGGCTGAAGTCTGTGCTACGACTTTTGCTAGTGAAAAAA CCTTCGGTTCGCTCACTAGCATTAAAACTTTTAGCATTTCATCTTACCGGTGAAGAAGGAGCTGATACAA AGCGCCCATTCGTAGATGCTAGAGTTCTTTCCAGAGTTACTAATCTGTTTATTGTGAAAAAACCTATTGA TCTTAAGTTGGATGACAGAAGAGAACTAGTTATTAAGTTGGAGACTGTTAAAAAGGTGTATGAAATCTTC ACCTCAGATGATGTTGATCTTGTTTTAAGAAAGTCAGCAGCAGAACAACTAGCTGTGATAATGCAAGATA TTAAAATGCATACTGTGGTGAAAAAGTTGTGCTTAATTGAGAAGATTATTGGATACCTAAATGAATTCGT GGGTCAAGATGGCAAGGTTATAGAATGCTTGATTCGGCCATGTCTCACACTCCTGAGGAAGGTTGTGTGT GCTGACCTGGTTATCCGTGCCTCTTTGTCACAGCAGGCCTCTCTCTTGACCCTCTTACTCAGAGTTTCCT TGATATTTCATGAAGACTGTGCTGTGGTGGCTGAAGTCGGGGCATTGTTTTGTCTTCTGTTGTTCGATGA AGTATCAAGAATGGATATGTGGTCTGATAATTCTTCTAATAAACCTTCTTTGTCATCAGTCTTCAGTTTG CCTGTTTCAGTCTTTAGAAGGTATCACCTACCAGTTTATGTGAATGGCCACCACGCTGTGAGTCCTTACT CAATAGTTTTGCCATTATCTACTGAATGTTTGGCCCTCAAGCCTGTGTCAGATATGCTGAGAATAGCTTG GAACCTGGCATGGTATCATGGGAGTGATAATCTATTGAAACAAACGACCTCTGAAACTAAAATTCAGGAA TTTTCAGATACACTGCAGCTGTCCACAGAAGATATCCTTACACTGAAGATCACATACACGGATAGTGGGC TGAAGGACTGCCTTCAGTCCATTGTTCAGGCTGGAGGCCACAGGGATGTGAGGGCTGCTATTACCAGGAT GAGTTTTTATCTTTTGAACGACAAACTGTCTTTGAAGGATGATATTGGTCCATGTGGGTTTACTTTAAAG TCCTTGGCCTGGCACACTGTGCTGAACAGGTTTTTACAAGTACTTCCTGCTTGCACTGAAGATGAAAAAC TGCTAATAGATATCATACATTTTTTGAATAAGTTAATAAAGGAACAAAGAAAAAATCCATCAGTAGAACT TTTAAACTGGATTCTAGAATTACTTCTTAGACATAATCCAAACCCACTATTAGACCTGCTGGTTCTGACA GAGTCACATACACGAGAAGTAACAGATGAAATCCGGACTGCTGTCAGACAGCAACTCCAGAAAGAATTGA TCACTCTTCTTAATTCCTTATTGCTGGTTTGCATGACAGTTTCTGACAGGAAATGCCTGGAACTATTTTA TGTTTTCCAAACACAGCTGTCTCTGAAGCTACTCCAGTGCCTCAGAGTCACAGATGCTCCCCATTTCTAT GGCTTGCCTTCCTTGGAGAGGACCTTAGCAGGGATGGCTCACCTCACTGCCTCCCCTGGGTGGAGTGCAC ATTCTCCTCTCTCAAAGCCACTTGAGATTTGTGGGAAGTACTTGTCAGGTCTCCTGGAGGTAATTACTTC TTTTTATGTGGAAAGGGGAGGAAACGCTATGTCCTTCATGGGGAAAGGTGTGACGAAGAGCACAGTTCTT TGCTTACTTCATTTATCCCACGAGATGATAGTCCAGGCACCAAACTCGGAGTGGATGCCACTTTGGTTCT TGCCTTTGGGTAGTCACAGTGAAGAAAACGTCCCTTCTCAGCAAGGATTGGCCTGGCTGATTCCCTTATG GGTTGATCGAGACCCAGAGGTGAGATTCACTTCACTGGGGTTGGGATCAGCACTGACTACTATGGAAGCT GGCTGTGTGGCCCTGGCAAACAGTTGTCAGAATATTTCTGGTGGGCTCTGGGGAACTGTGGTGAACATTC TTCTGGATCAGTCAGAATGCAGTATGGTGCGGCGGGAGGCTGCATTTATACTTCAGAATCTCCTTGTAAT TCCAATGCCTTCAAAGGTTATAAAGGATTATACTTGGCAGGGCCCCTGTGTCCATGATGAAGATTCTGGC CTGTCACTTTCAGGAAAACCTGCACTACAGGCCCTTTTGTATCACTGCCGTTTCTATGAACATTTGACTC ACATGGTGAAACATTGTTACGTTGGAAGATATATGTTTGATTTTAATATTTCTGCTTTTACTGAAACTTC AGAATATAATGATTTAAATGGTTTAGATGACTCATTCAAGTTCTGGAGGGCCCCCTCTGGGACATCTACT CAGGATCATGAGCCAAGTTCTCTTTCTACCTCAGAAACAATGGTGGCGGCTTCGGTGGTCAGTGCTGAGA TTCAGCCCCTGCTGCCATCAACACTTCTTCCTGAACCAGCAGCCCTTGACCAGTTTGTGACACAAGGGCA CCTGCACTCAGTCAGACCTGTGCGACCGCGTGACTCATCGCCGTCTGCTTCCCTGCACAGGCAGTGTGTG TTTGTTACTCCGTCTCTGCTGTCAGCAGTGTGTAGCCTCTTAGACAACCTCTTGGCAGTTACTCCAAGAG ATGCTGCAAATGCTTTTCAACAAGCTCATCTCATAGAACTCCTCTGTAGCACTATGGATGCCTCCCTCCT AGAAATGTGCGTCCAGGAACTCAAGACCCCACTGTCTTTATCATCTGCTGTTGAACATACTCAGGCTCAG ACTTCCTTTTTTTTGGAGTACCTATCCTCTTTGTTCCAGCTTTTGAAGTCCTGTCTGTTGGTAGATCCTA AACTTGTGACACAAGATGAGCTCTTGAAACCTCTTATCACTAATGTCATCCGAGTTCTCATTGTGTGTAC CAAAGATGTATTGGATGTACAGTTAGTGTTGGCTTTTCACCGCACATGGACACATTTATTTGACCTTCTG GCCACACTACTGAGGAAGGCTGGTGCTGTCTCCCTTGTTCCTCTTACCTCAGCTCTTGCCAAGCACTGGG AGGCAGTGACTGATACACTGTGCAGATGTGTGGGCCTCTCAGTCAAGTGTCCTGCCCTTAGTATTGCCAG CCTCCAGTTCCTGTCTGTTCTCTTGGCTGAGGAAGAAAAGAGACGTGTCCAGGACAAGGATAAAACAAAT GCATGTCAGACCCCAACAGTGGCCTTCCTTCTTGATGGAACTCAGGGAAGCCTTAGATCTTCAGAGCGAC TTAATGAAACAATTCTTCAGTGCTATGAAGGAATCTCCTCCAAAGACGTCCTGAAAAGAGTTGCTGCAAA TGCTCTGATGTCACTTCTGGCTGTCAGCAGAAGAGCGCAGAGGCATGCGTTGAAATCTGATCTCATAGAA AACTGTGTGGAGCAGATGAAGCACATCAATGCACAACTGAACCTAGATGTGCTGAGGCCTGGGAAAGCAG CACTAAAAAAAAAGGAGGATGGCATCATTAAAGAGTTAAGCATTACCCTGCAGCTCCTAAGAAACTGCCT TTATCAAAATGAAGAATGTAAAGTGGCAGCTTTTGAAGCACATCTTGTGCCTGTCCTGCACTCTCTCTGG CCTTGGCTTTTGATGGAAGATTCATTGATGCAAATTGCCTTGCAGTTGCTTTGTGTCTATACTGCAAATT TTCCAACTGGCTGTAGTTCCCTTTGTTGGTCAAATTATGGACAGTATATTCAAACCGCACACAGAGGCAC CCCCAGCAGCTCCCTGATACTGTGTGCCCTGAAACTGGCTTCTCAGGTGCCTGCAGAGAACACCCCCATT CAGCAGGCGGTTTTTATGTTTCTTTCAAACCTGGCTTTATCTCATGACTGCAAGGGAGTAATTCAGAAGA GCAACTTTCTACAGCACTTCCTGTCCCTAACATTGCCTAAAGGAGGAAATCAACATCTCAGCAATGTGAC CATTCTATGGTTGAAGTTGCTCCTGAATATGTCGTTTGGAGAAGATGGACAGCAAATGATCCTGAGACTG GACGGCTGCTTAGACTTACTCATCGAGATGAGCAAGTACACACACAAGAGCAGCCCTCACGTGCCTCTGC TGATCTTTCATAACATTTGCTGCAGTCCTGCTAATAAGCCTAAAATTCTGGCTAATGAAAAGGTTGTTAC TCTTCTGGCTACCTGCTTAGAAAGTGAGAATCAAAATGCCCAGAGGATTGGAGCGGCTTCACTTTGGGCT CTAACATACAATTACCAGAAGGCAAAAGCAACTTTGAAAAATCCATCAATTAAAAGAAAAATAGATGAAG CATATTCCATAGCAAAGAGAACTTTCTCAAACTCAGAAGAAGACCCTCTAAATTCCTACTATTTGAAATG CCTTGAGAATCTTGTACAACTGCTTAACTGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170436
Insert Size	6687 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170436.2, NP_001163907.2
RefSeq Size	7078 bp
RefSeq ORF	6687 bp
Locus ID	291377

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