Frem3 (NM_001191699) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1562701
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217743 representing NM_001191699 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGCATTCTCTGAACCTTTCTGGGATGTCCCCGCAGCTGCTAGTGGCTCTCGCCTGCCTGCTTC TGACTTGTGGTGCACTGAGAGAACAGGTACCCCTCAATGAAAACGTGTCCCACGGGGAGCTCTATCTGCC TCCTTGGGGAGCGCTCGATGGTGGCAGTCACCAGAACCCCGGTATTCTGATCACCAACCCTGGGTTCCAA GTGCCACAGGGCCGCTCGGTTTGGCTCGACCCACTGCGGGATCTGATGATTCAAGTGCAGCCAGGCGACC AGTGCGAGGTGACAGTGCTGGACATCCCACGGCTCCAAGGAGCGCTCTCCCCGCGCCAATTTCCCTGTGA TTTCGGGGCCCGCCAGGTCAAATACATTCACTTCGGCTCGCCCACCGCCACAAGAACCCGGATACAGCTG CAGCTGCGCTACGAGGCAGGGAATTCCACGCTGGTGCTGCCCTTTACTCTGCAAGTGAACGTAGTCTTCC CCAAGCTTCAGTTGGTAACGTGCAACAGGCCGTTGAAAGTGTTCAGACTGCTAGGCTGCAGCCAGGCCAT TGACCGGAGAGTGCTAAACTTCACCTCCAGCAGCAGGTGCAGGCTCACTTTGCTTCCTCACCCTGGCGGA CCACTCCCCAAGTATGGGCACTTGGTGGATGCCGCTGGGACCCCTCTCTCCAGGGGCCATATGGCGGACT GTGAGACTTTTATCCAGGCTGGGGTGCGCTACCAGCATACTGCCACGCCATCCTCACCCACCAGGGACTA TGTGCCCATGCTGGTGGAGCTGCTAGGGCCAGAGTCTCGAGATACTAGGTCAGCAAAGGTGCTGGCCCAG GAGTACTGCCAGCTTCAGGTCAGACTCCAGAAGGGAGCCAGGAGCACAGCGCCGAGACCCAGTTTCGCGG CTCCAATGATGGTGGAAGTTGAACAGTTCCTACTGACAGCCCTGACCCCTGATGCGCTGGCTGCAGAAGA CGGCGAGTCAGACCCTGACGACCTAGTCTTCAAAATTCTCAATGCCCCTGAGGCTCCACCCGGACACCAT GGTCGACAGGGTTCTGTGGTGAACACAGATGACCCTCTGGGCCTTCCAGTCTCTTTCTTTACCCAGAAAG AGTTGAGAGAGCTGAAAATTGCCTATCGACCCCCAACAGGGAGCTCAGAAGGGGACCTCATCTTTCAGCT GGTACTGCAAGTGGAGGATGAGGATGGTGACACCTCTGAGCCTTTTGTCTTCATGGTGGTGGTGAAGTCT TTGAATGCTCTGGCTCCAGCAGCTCGCTACAGTGCAGGACTTGCCGTTTATGAAGGCCAGGCAAGGCCCT TGTCCGATGCCCAAAACTTTCAGGTCAGTGATAATTCCAAAGAAATAAGAATTGCTGCAGTCAGAGGTTT GCAACACGGGCAGCTGGTGGTGCGTGGGGCACCAGTTGAGTGCAAGTGTTTCACACCAGCAGACCTAAGT GCAGGGCGAGTGGTGTACCAGCACGACGGCAGTGACTCTTACAGTGACAACGTCATCCTCGAGATGGAGG ATGGGCACCACCAGGTGGACTTCCTCTTCCCCATCACCATAGTGCCTGTAGACGATGAGGCACCAGTACT CACCACCAACACGGGGCTCTCAGTGACTGAAGGGCAAGCCATCCCGATATCCCCCTTTGTCTTATGTGCC ACCGATATCGACTCCGAGGACTCAGCTATCCTCTTTGTGCTGGAAGACCAACACCCGGAAGGAAAAGAGA AAAAGAGGGGTGAGGCTCTGACCCCCGGTTTCTACAGCTCAAACCAGTACACAGGCAAGATGCTGCTGAG GCAGGCTGAAGCCCCTTCGTCCCTTCTGCGCAGTGACTGGTACTATGTGGAAAAGGAGGGCCTTTATGAG AAGGCAGTGACTGAGTGGCTACAGAGAGATATAACGGAGGGGAGACTTTTCTTCTTCTACCTAGGACCAC ACAGCCCATCGACAGTGGCCCGTCTGGCTTTCCGTGTGCAGGATGACCATGACCCACCAAATCTCTCCAA CCAGCACCTCCTGACCATCAGCATCCAGCCAGCAGAGAAGCAGAGTCCTCAGCTATCTCCAGAAACTACC CTAGAAATGACTGTGCAAGGATACCAGCTCACTCCCTTCCAGAAGAAATTCCTCCAGTACATTGGCCAGA ACTCAGATGACCAGAACCTGTCGTATACTCTCCTGACAGGACCAACAGATATGGACAGCAACCACCAAGT GCCGGCTGGAGAAATCGTGCTCACCGATTCACCAAATAGACCCATCGTTCATTTTACCCAAGCCCAGGTC AATCATCAAAAAATTGCTTATCGGCCCCCACCGAGGAATCTAGGTATCGTACCTCGAGTTGTACAATTCA CCTACAAGGTAGAAGACATTGCAGGTAGCAGTGTACCTGGGACATTCACACTATTCCTGCAGCCTTTGAA CAGTCAGCCACCAAAAGTCATCACTAGAGGCTTTGCTGTCTTAGAGGGACATAGCTTTATTCTTGGCAGA GGCGAACTCAATGTTTCAGACCCTGACACCAATGTTGATCAACTTTTCTTCGTTTTAGTTTGGGGTCCCC AGCACGGACACTTGCAGTACTTTAAAAAGTATATGGTTCCAGGTGAATCGTTTATGCTAGCCGATGTTGT CAATGGGAATATCTCTTACCAGCACAGCAGAGACCAGACTACCAGTGACGTCATCTACCTAGAGGTAAGT GATGGAGTACACCACATACCCATCACTGTCCAGGTTTCCGTGCTGCCCACTGTGGCTAAAAGAAGTCCCG GGGTCTCTTTCACAGATACTCGAGTACTGGCTATTTCTCTAGATGTGCTTGAAAATGCAGCTTCTGAGGT CACTGTGGGTGTTACCCATGGCGGAAAGGATCTGATGCTATCCTTCATTGTGGAGGACAAGCCTAAACTG GGCATCATTCTGGTGAATGGTTTGCCAGCAGAACGAGTCACCCAGGAGGACCTCCTTGGTGGGGCAGTAA GCTATGCTCACACTGGAGGTGAAATCGGCTTCCGAGGGGTGTGCGACGTTTTCAGCTTCATCCTCTCCAC AGATGCCCGTCACCCGACCGTGGGAGACAACACACCAGAGAGGGTGCTGGTGGAAGTGACAGTGCTGCCC GTGGACAACACGGCCCCCAAGGTCTCGGTAGGAGAGCCCTTCGTTGTATATGAAGGTGAGAAGAACCCCT TGACCCCACAACATGTCAACATTGAAGACGTGGACACTTCCGAAGATGAAATCCTTTGCACACTGACTGC GCAGACATCCTCTGGCTACCTGGAAAACTTGGCACCAGCTCCTGGTTCAGAAGTGTCACAGGCTGGCAGC CCCATCAGTGCCTTCTTCATCAAGGACGTCCGAGTGGGGCGTATCAATTACGTACAGAGTATCCACAAGG GAGTAGAACCAGGAACAGATCACTTCACCTTCTATTGCTCCGACGGCGTCAACTTCTCCCCGAATGTCCT CCTCCCTCTAACCATCTTGCCCACCAATGATGAGCAGCCTGAACTTTTCACCCAGGAGTTTGTGGTATTA GAGGGGATGAGCCGGGTCATAGACACCTCACTGCTCAATGGGGTAGATGCTGACTTCCCACCAGAGAAGC TGCACTTCCGACTCACGGTCCTGCCCCAGCACGGACGGATCCTACAGCAGCTGGCCACAGGCAGCAAGCC CATCCACAGCTTTACCTTGCAGGAGATCCAAGAGCCCTCTTCCATTGTCTATGAACACGATGACTCACAG AGCACGGAGGACAGCTTTGAGGTCTGGCTGAGTGACGGCAGGCACAGCGCTCACAAAAAGGTGCCCGTTG TTGTCATTTTGGTGGATGATGAGGCTCCCCAGTTGGCCATCAATGATGGGCTGGAAGTAGAAACTGGACA CTCGGAGATCATCACAAATCGCATGCTCAAAGCCACAGATCCTGACTCAGATAACAAGAGTCTCAGTTTT GTTCTCCACTCTGAGCTTCAGCAGGGGCTTCTGCAACGACTGAAGAAGCCAGGAGGAGACGTGAGGAGCA ACCTCACCGTGGGAATGAACTTCACCCAGGATGAAATTGACAGAGACCTTATCTGTTATACCCACACAGG CCAGGGAGAAGCTGTTGACCTTCTCAAATTTGATGTGACTGATGGGGTTAACCCTTTGAGGGACCACTAT TTCTATATCACCATTAGCAACTCAAACAGTATCATCCCTGAGGTAGTCAGCAAAAGGGTCACCTTGACAG AAGGTAGCAGAGTGACCCTCACCACTGAACTGTTTAACACCAGTGCCATCCACAGCCCTGGTGAACAACT TCAGTACTGCATCAAACGAGCTCCTAGGACAGGCCACTTGGAAAGCTCTGACCAGCCCGGGGAACCCATA GCTTCTTTTACGCAGCTTCACCTGGCCAGCAACAAGATATCCTATGTCTGTATTTCAAAAGATGAAAGGA AGATGGATCACTTTGAGCTTAGAGTGACTGGGGGACACAGCTCTGTGTCCAGAGTCTTCAGGCTGTTCGT CACAGATCAAGACAACAAGAAACCTACCTTGACCATCCAAACACTAGTCCTGCAAAGAGGGGACAGCATA GTGGTCACTCCTTCTCAGTTGACAGTGGAGGATGAGGACACTCCAGCTGATTTTATTCTTTTTACCATCA CACAGGTGCCCATCCATGGCAGGATTTTGTATAATGGTAGCCATCCTGTGACCACTTTCACCAAGAAGGA CCTGACAGAGAATGTGATTCTCTATCGTCACGATGGCAGTGAGACAACCAAAGACAGTTTCTCCTTCACC GTGACAGATGGCATCCACGCAGGCTTCTATGTCTTCCCAGACACTGCCCTGGAGACACATGTGCCTCAGA TGGTGCAGATCCAGATAAGCCCCCTTATCGACAGGCTCCCACAGATTACTATCAACAGGGGAGCCAGGGC CTTGACGATCCTTCACACTGGACACGTGGGTTTCCTCATTACCAGCAAGTATTTACAAGCGACCCATCAG GATGCTCCCCACAGACTCCTAACCTATAAAGTGACCAGTGGACCAGAGCATGGCTACATTGTCAATGCTG GCCTTGGAGATGAGAGCACGCACACCTTCACACAAGCCGACATTGATGAGATGCAGATCTACTACATCCT GAACAAGGACCACGGCAGCGCGACAAGAGATACCTTCTACTTCTCTGTTGGAACCAGTGGAGGAAGCCAG TTAAGAAATCAACCTTTCCATCTGAAGTGGGCTTGGATTTCTTTGGAAAAAGAGTACTACATTGTGGATG AAGACTCCCCATTCTTCGAAGTGACCCTCACACGCAGGGGATACCTTGGAGGCACATCTGTCATTAGCAT TGGAACCAAAGGAGACACTGCCGAAGAAAATAAGGATTTTAAAGGAAAGGCTCCTACACGGGTCCAGTTC AATCCGGGCCAGTCTACAGCCACATGGAACGTGGGACTTATACCCGACACCAAGTATGAGACCTCAGAAA CGTTCCAGATCGTTCTCTCTGAACCTGACATGGCTGCACTGGAGTTTCCAGAGATGGCAACGGTTGAAAT TGTAGATCCTGAAGATGAATCAACAGTTTACATTCCGGAGGCAGAGTACAAACTGGCAGAGGCTGTTGGG GAGTTTCTGGTCCCTGTAAGAAGATCTGGAGACATTAGCCAGGAGCTCACAGTCATCTGCTCTACTCGTC CAGGTTCTGCCGCTGGCACAATTCCTTCCATGGAGCTATCTCTGTCTGATTACATCTCAAGACCAGAAGA TAATAGGAGCAGGCTCCACTTTGAGAAGGAGGAGCGCGAGAAGACCTGCCAGGTCCTGATTATCAACGAC TCCCTTTACGAAGAGGAGGAGTCCTTCAGCATTGCCCTGAGCCTGCCCATGGGAGGGCGGCTGGGAGCCC GATTCCCCACTGCCAGGGTAACCATCCTGGCTGACAGAGAGGACGAGCCCGCTCTGCACTTTGAATACTC CGAGTACCACGTTGAGGAAAGTGCTGGCTCTGTGGAGGTAGCCATCTGGAGAAGAGGCACTGACCTTTCG CTAGCGTCTTCTGTTGTCCTGCGCTCTAGGAGGACAGAGCAACAGGCAGCAGAAGCGGGAACAGACTATA TTGGTGTCAGCCAAAATCTGCACTTTCGCCCGGGAGTGAGTGTGCAGAGACTCCGAGTGACAATTCTTGA TGACCTCCCACAGCCTGCCTTTGAGGGTCCAGAGATGTTTGAATTGCTTCTCCAGATGCCTACAGGTGCA GTGCTTGGAGAACCAAATAAAACCACTGTTACCATCGATGACAGCACCACCAACTGTGAAGAGGAGTGTG TGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191699
Insert Size	6375 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191699.1, NP_001178628.1
RefSeq Size	6595 bp
RefSeq ORF	6375 bp
Locus ID	307767

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