Wnk2 (NM_001191556) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1307284
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217733 representing NM_001191556 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGTTGATGGAGGCCGGGCGCGGCACGGGGTCGG CGGGCATGGCGGAGCCGCGAGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCC CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGCCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG CCGCGCCCGCGCCTGCCGCGCCCGCTGCGCCTCCTGGCTCCCCGAGTGCCCCCTCGGACCCTGGCCCGGA GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCGTCAGCTGCAGCCACCCCGGTCCCCGAC GGCGGCCCGCGGCAGGAGGAGGCGCCCGCCCCAACCCAGGAGGACACTGGAGCTACCGAGGCAAAGCCGG AGCCCGGGCGCGCACGCAAGGATGAACCTGAGGAGGAGGAGGACGATGAGGACGACCTCAAGGCTGTGGC TACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAAC TCGAACGGCAGCGGTTCAAAGAGGAGGCAGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAACGATGTATTGTGCTGGTGACGGAGCTGATGACCTCA GGGACATTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTACTGAGAAGCTGGTGCCGGC AGATCCTGAAGGGCCTGTTGTTTCTGCACACAAGGACTCCCCCCATCATCCACCGAGACCTCAAGTGTGA CAACATCTTCATCACCGGGCCCACCGGCTCCGTGAAGATTGGCGACTTGGGTCTGGCCACCCTGAAAAGA GCATCTTTTGCCAAAAGTGTAATAGGTACCCCCGAGTTCATGGCACCCGAAATGTACGAGGAGCATTACG ACGAGTCGGTTGATGTCTACGCTTTCGGGATGTGCATGCTGGAGATGGCCACCTCAGAGTATCCCTATTC GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAGCCGGCCAGCTTTGAGAAG GTGCATGATCCTGAGATCAAGGAGATCATCGGAGAGTGCATCTGCAAGAACAAAGAGGAGAGGTACGAGA TTAAGGACCTGCTGAGCCACGCCTTCTTCGCGGAAGACACGGGCGTCCGGGTGGAACTTGCAGAGGAGGA CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTAGAAGACCCCAAGAAGCTGAAGGGCAAGCCC AAAGACAATGGAGCGATAGAGTTTACCTTTGACCTGGAGAAGGAGACCCCCGACGAGGTGGCCCAAGAAA TGATTGATTCCGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGGGACCGGGTAGC ACTCATTCAATGGCGTCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACACAGGCAGC CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCATGCACAGAGCGGGCAGC CTGGACAGCCAGAACCTGAGGAGCCTGAGGCGGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC AGCCAGCATAGCATGGTGCTCAGCTCCCTCGTGGACACAGCTCCAACCCCAGCCTCGTGCGTATGCAGCC CCCCTGTGAGTGAGGGGCCCGGCTTGACACACAGCCTGCCTAAACTAGGGGCCTTCCAGCAGCCAGCCGC TGTGCCTGGCCTATCAGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA GAGCCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC CAGCACCCCCAGTTCAGCAGCCTCTTCCAATGGCCCAGCCACCTACCCTGCCGCAGGTCCTGGCCCCACA GCCCATGGGCACTGTCCAGCCAGTGCCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTAGTG GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTACAACCTCTTGCTCAAGTCCCTCCGC AGATGCCTCAAATGCCTGTGGTTCCCCCCATCACTCCCTTAGCAGGACTCGACGGCCTCCCTCAGACCCT CGCTGACCTGCCGGCTGCAAACGTGGCCCCTGTGCCGCCACCTCAGTATTTCTCACCTGCTGTGATCTTG CCAAGCCTCACAACGCCCCTGCCCACATCACCGGCCCTGCCTCTGCAGGCTGTCAAGCTGCCTCACCCCC CTGGGGCACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAACGCACCAGCTGCTATCCCCCTCTTGGC CGTGGCCCCGCAGGGTGTGGCCGCACTGTCCATCCATCCAGCAGTGGCCCAGATCCCAGCCCAGATCCCA GCCCAGCCTGTGTACCCAGCAGCCTTCCCAGGAATGGTACCTGGAGACGTCCCTCCCTCACCCCACCACA CAGTGCAGAGCTTACGGGCCACCCCTCCACAGCTGGCCTCGTCTGTGCCTCCTCAGTCTGTCCAGCCCAG TGTCGTTCATCTACCTGAGCAGGCTGCTCCATCTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCT CCTTCGTACACCACGGATGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTC CTCTGCCAGATACTCTGCTGCCTACTGTCCCTGAGCTCCTGCCCAAGCTCCCCAGCTCTCTGGCCCCTAC GGTGGTCGCAGCTTCTCAGAGTGTGCCCACACAGACCTCTACACTCCTCCCACCAACAAACCCACCTCTG CCCACTGGGCCTGCAGTCGCTGGCCCCTGCCCTGCTGTCCAGCTGACGGTGGAAGCAGCTCAAGAGGAGC AGGTCTCACAAGACAAACCCCCGGGCCCCCCACAGAGCTGTGAGAGCTTGGGAGGTTCTGATGTCACCTC TGGAAGAGATCTGAGTGACAACTGTGAAGGCACCTTTGGAACGGGCAGGCTGGAGGGAAGGGCTGCCCGG AAACACCACCGTAGGTCCACTCGTGCACGCTCCCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCC TGAATGTGTGTAACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAGACGCATAACCACAAGATGGTGAC CTTCAAGTTTGACCTCGATGGGGACGCTCCAGATGAGATTGCCACATACATGGTAGAGCATGACTTCATC CTGCCCGCTGAGCGAGAGACGTTTATCGAGCAGATGAAGGACGTCATGGACAAGGCAGAAGATATGCTCA GTGAGGACACGGATGCTGATCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCT GACCACGGGGGAGGAGAATGGACAGTCCCAAGCCAATGCCCCTGTCTATCAGCAGAATGTCTTGCACACG GGGAAGAGGTGGTTTATCATCTGTCCAGTGGCTGAGCACCCAGCAGCAGACACTTCTGAGTCATCACCCC CACTTCCTCTAAGCTCTTTGCAGCCAGAAGCCAGCCAAGACCCAGCACCCTATCCAGACCAGCTGTCCTT GACGGACAAACCCAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGT GGAGCATCAGCCCCCACGGCCCCATCTTCCCCTCCTGTGACTTCTGTAATGCCCGCAGCACCAGCCACCA GCACCGTGCCAGAGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGC CAGTGTTCATGAGACCCTCCACCCACTGGCCGAGACACATCGTGCCCAGGGTGCTGCCCAGCCCCTTAGC ACTGGCCAAGGACCTTGCACTCCAGCTCTGGAGGCCTCTCACTGCTCCACAGGTCTGGGAGAGCCCATCT CAACCAAGGAGGTCAGCACTCAAGGAGGGACCCTGTCTGCTTCAATGCCAGAGCCCAGCCCTCCCACTGG GGCTGTACAATCTGTACCGAGTCAGCCACCACCCCCACTGCCCATCACAGTGGGGACTATAGGTCTGGCA GCTCCCCAGCTTCCCAGTCCCCCTCTGGGGCCTACTGCTCCCCCACCACCACCCTCAGCCTTGGAATCTG ATGGGGAAGGGCCACCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCG GACTCTGCTCTACCAGGAGCATGTGCCCACCTCCTCAGCCTCGGCTGGGACCCCCTTGGAGGCAAACGAC AGAGACTTCACCCTGGAGCCCCTTCGAGGAGACCTCCCCTCGGCCTTGAGTGACAAGACCCCAAGCCTCA CCCAGCAGAGCCAGCCCTCATTGGAAAAGTCAGAAACGGCTCCTGCAGGGTGGGCCCTGGCCCCGCGGGA ACAGGGTGCGTCCTCTTCAATGACAGCAGAGTCCTCTTCCAGCAACACACTGGGCTGTGACAGTGACACA GGGCAGGTGGCCTCGGACTCCCCCACAGCACCCAGTGTCCCCCAGGATACCTCCGTTTCTTCCGTCCCTA CGCACATGGATCCCAAAGATCAGAATGGTAGTGCTCCCAGAGAAACCTTGGTAGCACCCATGCAGAGTTG CCCAGGGACCTTCACAGAGGGCAGCCCAGCCCAGCTGCGTGGCGTCCGGGACTCCGGGTCCCCCCATAAG CGGCCAGGGCAGCAGGACAGCAGTTCTCCAGCCAAAACTGTGGGCCGCTTCTCCGTGGTCAGCACACAAG ACGAGTGGACTCTGGCGTCACCTCACAGCCTGCGGTACTCCGCCCCACCTGATGTCTACCTGGATGAGAT CCCCTCTAGCCCTGAGGTGAAGCTGGCAGTTCGTCGAGTACAGACAGCCTCCTCCATCGAGGTTGGCGTT GAGGAGCCTGCCTCCAGTGACTCTGGGGATGAATGCCCAAGAAGGAGGACCCAGGTGCAGAAGCAGTCCT CCCTGCCGGGGAGCGGTGGCGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCATC AAGGGCTGGCTCGCTGGGCCCTGAGACACCCAGCAGGGCAGGTGTGAAGGTCCCTACCATCAGTATCACC TCCTTCCACTCCCAGTCATCCTACATCAGCAGTGACAACGACTCGGAGTTTGAGGACGCAGACATAAAGA AGGAACTGCGCAGCCTGCGGGAAAAGCACCTGAAGGAAATCTCTGAGCTACAGAGCCAGCAGAAACAGGA GATTGAAGCCCTGTACAGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACCGCACCC CCTATGGGCCGCAGGAGAAAAACCAGCAAGAGCAAATTGAAGGCGGGCAAGCTGCTGAATCCCCTGGTGC AACAGCTCAAGGTCGTGGCCTCCAGCACAGGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAG CAGTAGGTCTTCCACCAGCAGCCTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAG GCGCAGGTGAACAACAGCAACAACAAGAAGGGCACCTTCACGGATGATCTGCACAAGCTGGTGGATGAGT GGACAACGAAGACAGTGGGGGCGGCCCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCT GCATGACATGGAGGCCTCTGGAGAGGCTCGGGCTACATCTGTGCCTCGAGGAGGAGTGGCAACATCGTGT CTGGCCCCAGCACCTGGCCCTCTGTCCAGCACAGCCATTCCTGGAGTCACCCCAGCCCTGCCTGTGCCCA CACCAGATCCTGAGAGTGAAAAGCCCGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191556
Insert Size	6192 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191556.1, NP_001178485.1
RefSeq Size	6450 bp
RefSeq ORF	6192 bp
Locus ID	306811

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