Myo18a (NM_001172137) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217731 representing NM_001172137 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACCTCATGAAGAAAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAGAAAAAGGAGA AGAAGGAGAGGATGTCTGCGGCAGAACTGCGGAGCCTGGAAGAGATGAGCATGCGCCGTGGCTTTTTCAA CTTGAACCGGTCCTCCAAGCGGGAATCCAAGACGCGCCTGGAAATCTCCAACCCCATCCCTATCAAGGTG GCCAGTGGCTCTGACCTGCACCTAACCGACATTGACTCTGACAGCAATAGGGGTAGCATCATCCTGGATT CAGGCCACCTAAGCACAGCCAGCTCCAGTGATGACCTCAAGGGTGAAGAAGGCAGCTTCCGGGGTTCTGT GCTGCAGCGGGCAGCTAAGTTCGGTTCACTAGCCAAGCAGAACTCTCAGATGATTGTCAAACGTTTTTCC TTTTCTCAACGGAGCCGGGATGAGAGTGCCTCAGAGACCTCAACCCCCTCAGAGCACTCTGCAGCCCCAT CACCTCAGGTAGAGGTGAGGACGCTTGAGGGACAGCTAATGCAGCACTCTGGGCTAGGCATCCCTCGACC AGGACCCAGGTCTCGAGTCCCTGAGCTGGTGACTAAAAGATTCCCAGCTGACTTACGTCTCCCGGCTCTG GTGCCCCCACCACCTCCTGCTCTCCGGGAGCTGGAGTTACAACGACGACCCACAGGAGACTTTGGCTTCT CACTGCGGCGCACAACCATGCTGGACCGGGCCCCTGAGGGTCAGGCCTATCGACGTGTGGTTCACTTTGC TGAGCCAGGGGCAGGCACCAAGGACCTGGCCTTGGGGCTGGTGCCAGGAGACCGACTGGTGGAGATTAAT GGACAGAATGTGGAGAATAAGTCCAGGGATGAAATTGTGGAGATGATCCGGCAGTCTGGGGACAGTGTGC GGCTCAAGGTACAGCCCATCCCAGAGCTTAGCGAGCTGAGCCGGAGCTGGCTTCGGACTGGCGAGGGACA CCGCAGGGAGCCGACTGATGCCAAGACAGAAGAGCAGATTGCCGCTGAAGAGGCCTGGTATGAGACGGAG AAGGTGTGGCTGGTCCATAGAGATGGATTCTCCTTAGCCAGCCAGCTCAAGTCGGAGGAGCTCAGCCTAC CCGAGGGGAAGGTGCGCGTGAAGCTAGACCACGATGGAGCCATCCTGGATGTGGATGAAGATGACGTAGA GAAGGCTAACGCTCCCTCATGTGACCGACTAGAAGATCTGGCCTCCTTGGTGTACCTCAACGAGTCTAGC GTCCTGCATACACTGCGCCAGCGCTATGGGGCCAGCCTGCTACACACCTATGCCGGCCCTAGCCTGCTTG TCCTTAGCCCCCGAGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATGCACATGTTCAAGGGGTGTCGGCG GGAGGACATGGCACCCCACATCTATGCTGTAGCCCAGACTGCATATAGGGCCATGTTGATGAGTCGTCAG GACCAGTCCATCGTCCTTCTGGGCAGTAGTGGCAGTGGCAAGACCACCAGCTCTCAGCATCTGGTGCAGT ATCTGGCTACCATTGCCGGCACCAGCGGGAACAAGGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGGCCAC CCTCCTGGAAGCCTTTGGGAACAGCCCTACCATCATGAACGGCAGTGCCACCCGCTTCTCCCAGATCCTC TCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTTCAGCCTCCGTCCAGACCATGCTCCTGGAGAAGCTGC GTGTGGCCCGACGCCCAGCCAGCGAGGCCACTTTCAATGTCTTCTACTACTTGCTGGCCTGTGGGGACAG CACCCTCAGGACAGAGCTCCACCTGAACCACTTGGCAGAGAACAATGTGTTTGGAATTGTGCCATTGTCC AAGCCTGAGGAGAAGCAGAGGGCTGCTCAGCAGTTCAGTAAGCTACAGACGGCCATGAAGGTGCTGGCCA TCTCCCCTGAGGAACAGAAGGCCTGCTGGCTCATCCTGGCTTCCATCTACCACTTGGGGGCTGCTGGAGC TACCAAAGAAGCTGCTGAAGGTGGACGTAAGCAATTTGCCCGTCATGAATGGGCCCAGAAGGCCGCATAC CTGCTAGGCTGCAGCTTGGAAGAGCTGTCCTCAGCGATCTTCAAGCACCAGCTCAAAGGCGGCACCCTGC AACGGTCTACCTCCTTCCGTCAGGGCCCTGAGGAGAGCGGCCTGGGAGAGGGTACAGGCACCAAGCTGAG TGCTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCCTCTACAGCGAGCTCTTTACTCTCCTCATCTCCCTG GTAAACAGGGCTCTCAAGTCCAGCCAGCACTCACTGTGTTCCATGATGATTGTGGATACACCAGGCTTCC AGAACCCTGAATGGGGTGGCTCAGCCCGTGGCGCCTCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGA CAGGTTGCAGAAGCTGTTCCATGAGCGCACCTTCCTCCAGGAGTTGGAAAGGTACAAGGAGGACAACATC GAACTGGCGTTTGATGACTTGGAGCCAGTCACAGATGACTCAGTGGCTGCTGTGGACCAGGCCTCCCACC AGTCCCTGGTTCGCTCACTGGCCCATGCCGATGAGGCAAGAGGACTACTGTGGCTCCTAGAAGAGGAGGC TCTGGTGCCAGGTGCGACTGAGGATACCCTCCTGGACCGTCTTTTCTCCTATTATGGCCCCCAGGAAGGT GACAAAAAAGGTCAGAGTCCTCTTCTGCGCAGTAGCAAACCACGGCACTTTCTCCTGGGCCACAGCCATG GTACCAACTGGGTGGAGTACAATGTGACCGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAA TGCACCCCGGCTCCTGCAGGACTCCCAGAAAAAGATCATCAGCAACCTGTTTCTGGGCCGGGCAGGCACT GCCACAGTGCTCTCGGGCTCAATTGCAGGCCTGGAAGGTGGCTCCCAGCTGGCCTTGCGCCGGGCAACTA GCATGAGGAAAACCTTTACCACTGGCATGGCAGCTGTCAAGAAGAAGTCACTGTGCATACAGATCAAGCT GCAAGTGGATGCCCTCATCGATACCATCAAGAGGTCCAAGATGCATTTCGTGCACTGCTTCCTTCCTGTG GCCGAGGGCTGGCCTGGGGAGCCCCGATCTGCTTCTTCTCGCCGAGTTAGTAGCAGCAGTGAGCTGGACC TGCCTCCGGGAGACCCCTGCGAGGCTGGGCTGCTACAACTGGATGTGTCCCTGCTCCGTGCCCAGCTTCG GGGATCCCGCCTGCTTGATGCAATACGCATGTACCGCCAAGGTTACCCAGACCACATGGTGTTTTCTGAG TTCCGTAGGCGCTTTGATGTCCTGGCCCCACACCTGACCAAGAAACATGGGCGTAACTACATCGTGGTGG ATGAGAAGCGGGCTGTGGAGGAGCTGCTGGAGTCCCTAGACCTGGAAAAGAGCAGCTGCTGCATGGGCCT GAGTCGGGTATTCTTCCGGGCAGGCACGTTGGCACGGCTGGAAGAGCAACGAGACGAGGAAACCAGCAGA CACCTGACCCTGTTCCAGGCAGCCTGCAGGGGCTACCTTGCCCGCCAGCACTTCAAGAAGAGAAAGATCC AGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAAGAAGAACAAGGGGGTGAAGGACTGGCCCTGGTG GAAGCTCTTCACCACTGTGCGGCCCCTCATCCAAGTTCAGCTGTCTGAGGAGCAGATCCGAAACAAAGAC GAGGAGATCCAGCAGCTGCGAAACAAGCTTGAGAAGGTGGAGAAAGAGAGGAATGAGCTTCGGCTCAACA GTGACCGGCTGGAGACCCGGATCTCAGAGCTGACATCGGAGCTGACTGATGAACGCAACACAGGAGAGTC CGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGGCTCCGGACCGAGAAGGAAATGAAAGAGCTACAG ACCCAGTACGATGCCCTGAAGAAGCAGATGGAGGCGATGGACATGGAAGTGATGGAGGCCCGGCTCATTC GGGCAGCCGAGATCAACGGGGAGGTGGATGACGATGACGCAGGGGGAGAGTGGCGGCTGAAGTACGAGCG AGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTTCAGCAGGAGCTGGAAGACAAGATGGAGGTGGAG CAGCAGAACAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCTGCAAGCAGATAGTGACGAGAGTCAGCGGG CACTGCAGCAGCTCAAGAAAAAGTGCCAACGGCTCACAGCTGAGCTGCAGGACACCAAGCTGCACCTGGA GGGCCAGCAGGTCCGAAACCATGAGCTGGAAAAGAAGCAGAGGAGGTTTGACAGTGAGCTCTCCCAGGCA CATGAGGAGACCCAGCGGGAGAAGCTGCAGAGGGAGAAGCTTCAGCGGGAGAAGGACATGCTCCTAGCTG AGGCCTTCAGCCTGAAGCAGCAGATGGAGGAAAAAGACTTGGATATCGCAGGGTTTACCCAGAAGGTTGT TTCCTTGGAGGCCGAGCTTCAGGACATTTCTTCTCAAGAGTCTAAGGATGAGGCTTCTCTGGCCAAGGTC AAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGGATCAGGAAGAGGAGCTGGATGAACAGGCTGGGA GCATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGCGGATGAGACAGACCCATTC CAAGGAGATGGAGAGCCGGGATGAGGAGGTGGAAGAGGCCCGGCAGTCATGTCAGAAGAAGCTAAGGCAG ATGGAAGTTCAGCTGGAGGAGGAGTATGAAGACAAGCAGAAGGCGCTGCGGGAGAAACGGGAGCTGGAGA GCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCGGGACTTTGAATCAGAGAAGCGGCTACGGAAAGA CCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCTCAGATCATGCTGGACCACTTAAAGAACAATGCCCCC AGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGGAAGAGTCGGAATTCACCTGTGCAGCAGCTGTGA AGGCACGGAAAGCAATGGAGGTGGAGATGGAAGACCTGCACCTGCAGATCGATGACATCGCCAAAGCCAA GACAGCGCTGGAGGAGCAACTGAGTCGCCTTCAGCGTGAGAAAAATGAGATTCAGAACCGGCTGGAAGAG GACCAGGAGGACATGAATGAGCTGATGAAGAAGCACAAGGCGGCCGTGGCTCAGGCCTCCCGGGACATGG CACAGATGAATGATCTCCAAGCTCAGCTCGAAGAGTCCAACAAGGAGAAGCAGGAGCTACAGGAGAAGCT ACAAGCTCTGCAGAGCCAGGTGGAGTTCCTAGAGCAGTCCATGGTGGACAAGTCCCTTGTCAGCAGGCAG GAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGTTCGAAAAGACCCAAGTGAAACGTCTGGAGAGCA TGGCCAGTCGGCTCAAAGAAAACATGGAGAAGCTGACTGAGGAACGGGACCAGCGCGCTGCAGCTGAGAA CCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAGCTCCGTGACACCAAGGAGGAGATGAGCGAGCTT GCCAGGAAGGAAGCTGAGGCTAGTCGCAAGAAGCACGAACTGGAGATGGACTTGGAGAGTCTGGAAGCCG CTAACCAAAGCCTGCAAGCTGACCTAAAGCTGGCGTTCAAACGCATTGGGGACTTGCAAGCCGCCATTGA GGATGAGATGGAGAGTGATGAGAATGAGGACCTTATCAACAGTTTGCAGGATATGGTGACAAAATATCAG AAAAGAAAGAATAAACTTGAGGGCGACTCAGACGTGGACTCAGAGCTGGAGGACCGGGTCGACGGTGTCA AGTCCTGGTTGTCAAAGAACAAGGGACCCTCTAAGGCACCTTCCGACGATGGCAGCTTAAAGAGTTCCAG CCCAACCAGCCACTGGAAGTCCCTCGCCCCCGACCTGTCGGATGATGAGCACGATCCTGTGGACAGCATC TCCAGACCCCGGTTCTCCCACAACTATCTGAGTGACAGTGACACAGAGGCCAAGCTGACAGAGACCAACG CATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001172137
Insert Size	6165 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001172137.1, NP_001165608.1
RefSeq Size	6951 bp
RefSeq ORF	6165 bp
Locus ID	360570

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