Heatr5b (NM_001191064) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217728 representing NM_001191064 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTAGCTCACAGTTTATTGCTAAATGAAGAAGCGTTGGCTCAGATCACGGAAGCAAAGAGACCAG TGTTTATCTTCGAATGGTTAAGATTTCTTGACAAAGTCCTAGTTGCTGCTAACAAGACAGATGTAAAAGA AAAACAGAAAAAGCTTGTTGAACAATTAACTGGACTAATAAGCAGCTCGCCTGGACCACCTACTCGAAAA CTGTTAGCTAAAAACCTCGCAGCCCTCTATAGCATTGGGGATACTTACACAGTTTTCCAGACACTGGATA AATGTAACGACATTATCAGAAGTAAGGATGACACTGCGGCCTACTTGCCAACAAAATTGGCAGCAGTGGC TTGTGTTGGAGCATTCTACGAGAAGATGGGAAGGATGCTGGGCAGTGCATTTCCAGAGACAGTGAACAAT CTGTTGAAATCTCTGAAAAGCGCAGAGTCTCAAGGGCGAAGTGAGATCTTAATGAGTCTGCGGAAAGTGT TGACTGGATTGGGTGGTGCAGCAGCTTCCTCTCATCGAGACATTTACAAGAACGCTCGATCCCTCCTCAC CGACAGGTCGATGGCTGTCCGATGTGCAGTGGCCAAGTGCCTTCTGGAACTACAGAATGAAGCTGTTTTC ATGTGGACTGCTGAACTAGAAAATATAGCTACCCTCTGCTTCAAGGCTCTGGAAAACTCAAATTATGGGG TGCGAGTCGCAGTGTCCAAACTCCTAGGGACAGTCATGGCTACAGCACTGATGCCAAAGCAGGCAACAGT CATGCGTCAGAACGTGAAGCGAGCAACGTTTGATGAAGTCTTGGAACTCATGGCCACGGGATTTCTGCGT GGAGGGTCAGGTTTCCTAAAGAGTGGTGGAGAAATGTTGAAAGTTGGAGGGTCTGTTAATCGGGAAGTGA GAGTTGGAGTGACACAGGCCTATGTTGTCTTCGTGACGACTTTGGGTGGCCAGTGGCTGGAGCGTAGCTT TGCCACGTTCTTGTCCCACGTACTGGATCTGGTTTCCCATCCTCGAGCAACACAAACACATGTGGACGCT GTGTACTCAAGGCGATGTGTCTCCTTCATCCTGAGAGCCACTGTGGGCAGTTTGCTGGGTGAGAAAGCCC AGATAGCAGCTGCCAAGGAAATCTGCCAGGCCATCGGGAAGCAGATGAAAGCCGTGGAAGCAGTAGTGAA CGATACAAGCAGTGAGAACAAGTCTGGGGCCGCAGACATCGCCGCCAGCCAGCACGTCATGGTCTGTGCC CTGCAGGAGCTCGGGAGCCTGGTGCAGAGCCTGAACGCCACCGCCTCCCCTCTTATTCAGGAAGCATCCA TAGGGCTTTTGGAGATTGTGACCTCAGTGCTCCTTCACCCAAGCATGGCTGCTCGGCTGGCTGCTGCATG GTGTCTGCGTTGTGTGGCGGTGGCCTTGCCTTTCCAGTTGACACCATTTCTGGATAGATGTGCAGAGCGG CTCAACAACCTCAAGACGTCCCCAGAAGCTGTTAGTGGATACAGTTTTGCAATGGCTGCTTTGTTGGGAG GCGTGCATCAGTGTCCGTTGGGCATTCCTCACGCCAAAGGAAAGATGGTGGTCAGCATTGCTGAAGATCT CTTACGAACTGCTGCTCAAAACAGCAGGCTGTCCCTGCAGCGCACTCAGGCTGGCTGGCTTCTTCTGGGA GCACTCATGACTTTAGGACCATCGGTTGTCCGTTACCACCTACCCAAGATGCTGCTGCTGTGGCGGAATG TGTTCCCTCGCTCCCTGAAGGAGCTGGAGGCTGAGAAGGCCCGAGGGGATTCTTTTACCTGGCAGGTAAC TCTGGAAGGTCGTGCTGGAGCTCTATGTGCCATGAGGAGTTTTGTTGCGCACTGTCCTGAGCTGCTCACT GAGGATGCGGTTAGGAAATTGATGACTCCTATTGAATGTGCCATGACCATGATGTCACACATTCCATCTG TAATTAAGGCCCATGGAGCCCACCTGAAAGCTAGTGCTGCAATGGTCCGCTTAAGGCTCTATGATATCCT GGCTTTATTACCTCCAAAAACCTATGAAGATCAAGAATCTCAGGTAAAGAGAGCATTTACCTACGTGTTA GAGCTGCTCCAGCCAAACAGTGCGTCTGGAAGCGGGGCTCTGGAACACGACCCTTCCTCCATTTACCTAC GCATACCTGCTGGTGAAGCCGTGCCGGGTCCTCTCCCCCTCGGGGTCTCCGTGATTGACGCATCCGTGGC TCTTTTTGGGGTTGTGTTTCCACATGTTTCTTATAAACACAGGTTGCAAATGCTGGATCACTTTGCTGAA TGTGTTAAGCAAGCCAAAGGTGTCCGCCAGCAGGCTGTGCAACTCAATATATTTACTGCTGTTCTTAGTG CACTGAAGGGCTTAGCTGAAAACAAGAGTACTTTAGGACCTGAGGAAGTTCGTAAGTCTGCTCTGACACT GGTTATGGGAGCTCTTGACAACCCAAACCCCATCTTACGGTGTGCAGCAGGAGAAGCCCTTGGAAGAATG GCTCAGGTGGTGGGAGAAGCATCTTTTATTGCCCGAATGGCCCAATATAGCTTTGACAAGTTGAAGTCAG CTCGAGATGTTGTATCCAGGACTGGTCATTCACTGGCCCTCGGTTGTTTACATCGTTATGTTGGTGGCAT AGGATCAGGACAACACCTAAAGACCAGTGTCAGCATTTTACTGGCTCTCGCTCAAGATGGGACATCCCCT GAAGTTCAGACTTGGTCTCTTCATTCACTTGCTCTGATAGTGGATTCTAGTGGCCCAATGTACCGTGGGT ACGTGGAGCCCACGCTGTCCCTGGTTCTCACCCTGCTGCTGACAGTCCCACCTTCACACACGGAAGTGCA CCAGTGTTTGGGCAGATGCTTGGGGGCGATCATAACTACTGTTGGACCTGAGCTGCAGGGAAATGCAGCG ACCATTTCCACCATCCGCTCCTCTTGCTTGGTGGGTTGTGCCATCACCCAGGACCACTCTGACTCTCTAG TGCAGGCAGCCGCCATCTCTTGCCTTCAACAGCTTCACATGTTTGCCCCACGACATGTCAATCTGTCCAG CCTTGTCCCTAGCCTTTGTGTTCACCTATGCAGCTCCCATCTGTTGCTCCGTCGAGCGGCTGTGGCCTGT CTCCGGCAACTTGCACAAAGAGAAGCAGCAGAAGTGTGTGAATATGCCATGAGCCTGGCGAAGAATGCAG GGGACAAAGAGATCAGCGGTGGCAATGTCAGTCCTTTCGCACCTGGAGTTAGTTCTCGCAGTGACATCCA TTGCCGGCACCAGGGTGTTAATATCACAGACACGGGGCTTGAGGGACTTCTTTTTGGAATGCTAGATCGA GAGACCGACCGAAAACTGTGCTCTGATATTCACGACACGCTGGGGCACATGCTTTCTTCCCTGGCAGTCG AGAAGCTCTCTCACTGGTTACCGCTCTGTAAAGACGTCTTAGCAGCTTCCAGTGATATGAGTGCGGCAAC GCTCTTAAGCAGTGGGAAAGATGAAGAGTCTGAGAAGAAAGATGAGATGGATGACGACGCCATGTTCACC ACACTAGGCGAGGAGGACAAGTCAAAGCCTTTCGTGGCTCCTCGATGGGCCACTCGGGTGTTTGCCGCAG ACTGCCTATGCCGAATCATCAACCTGTGTGAGAACGCAGACCAGGCTCACTTCGATCTTGCCTTGGCACG GTCTGCTAAACTACGAAACCCAAAAAATGACCTCTTGGTATTACATCTCTCTGACCTGATTCGAATGGCA TTCATGGCTGCAACTGATCATAGCAACCAACTGCGAATGGCTGGGCTCCAGGCACTTGAAGACATTATCA AGAAATTTGCATCTGTGCCTGAGCCAGAATTTCCAGGTCATGTGATCCTGGAGCAGTATCAAGCTAATGT GGGAGCTGCCCTAAGACCAGCTTTTTCACAAGATACGCCATCAGACATAATAGCAAAAGCTTGCCAGGTG TGCAGCACGTGGATTGGAAGTGGTGTTGTCAGCGATCTCAATGACCTCCGTCGGGTGCATAATCTACTCG TTTCCTCTCTGGACAAAGTTCAGGCTGGAAAGGGATCTCCTAGTCAACTGTACCGAGAGAGTGCCACCAC CATGGAGAAGCTGGCTGTTCTTAAGGCGTGGGCAGAGGTATATGTGGTTGCTATGAATATTAAGAAAGAA GCAGAGTCAAAACCGAAGAGAGCAATGAAAAATACTGATGATGATGATGATGACTTTGGCACCATAGACG AACTACCTCCCGACAGTTTAATAACCCTAGTGCAGCCCGAGCTGCCAACTCTCAGCCGCCTGTGGCTGGC CGCGTTGAAAGACTACGCACTTCTGACTTTACCAGCTGAGTTCTCTAGTCAGCTTCCCCCAGATGGAGGA GCATTTTACACTCCCGAGACCATCGATACAGCGAGACTTCACTACCGGAACTCCTGGGCCCCAATTCTCC ATGCAGTGGCACTTTGGTTAAACAGCACAGGATTTACATGTCAGGAGTCCACAGAAGCCACAGCTGTATC TGGAGGACAGAAGCGCTCCCCGGCTGTCAGTTTAAACCAGGTTCCAGGAGCCATGGCTAGTGCTAAACCC CTGCCAGAAGTTAACAAAGACAGAATGCACCTGATCCTAGGTGTGAGTATACAGTTTCTTTGTTCCCCTC GACCAGAGGAGCCCATTGAACACGTTACAGCATGCCTGCAGGCCTTACACACCTTACTGGGCTCTCCTTA TGCACGAATACACATTGCAGAAGATCAGCTGATTGGTGTTGAGTTGCTAAGTGTCCTGCACCGCCTGTTA CTGACCTGGAACCCACCGTCCATCCAACTGCTGGTCACTGGAGTCGTGCAGCAGATTGTCAGGGCCGCGC AGGACTATCTACAAGAAAAGAGAAGTGCTCTAAATGAAGAGGATATGGAAAAAGAGTCCTGTCCTGCACT AGGAGAAGGGGGTGACACCGGCGGCCTCATTCCTGGAAAGTCCCTTGTGTTTGCAACCATGGAGCTGCTG ATGTTCATTTTAGTACGGCATATGCCACACCTGAGTACCAAGATGTCGGACTCTCCAAGTCACGTGGCCA CAAAGACTCACCTGTCCGAAGAAAGCGCTCGCTTGGTGGCAGCCACAGTGACCATTCTCTCAGACTTGCC ATCACTCTGTTCACCAGCTGGATGTATGACAATCCTGCCTACAATTCTGTTCTTAATTGCAAGAATATTG AAAGACACAGCAATAAAATCTGTGGACAACCATGTTTCTCCCCCAGTCAGTGCAGCTCTTCAGGGGATAA AAAGTATTGTGACACTTTCTATGGCCAAAACAGAGGAAACTCAGAAACAGTGGACAGCTCTAATCCGGAG CACTCTTGCCTGCATCCTGGAATATTCCCAACCAGATGACTGTATGCCCGCACCGGATGAAGTGAGCACA CTTACAGCCATTGCGCTCTTCCTGTGGTCTGCCAGCAGTGAGATAATAGGAGTGCAGTCCCTACAGAACG GCTGCATGAACAGATTTAAAAGTGCCCTGAACTCGTGTGACCCATGGGTTCAAGCCAAATGCTACCAGCT TCTCCTCTCAGTCTTCCAGCATTCCAACCGTGCGCTCTCGACTCCCTACATCCACTCCTTAGCTCCACTA GTGGTCGGGAAGCTGAAAGCTGTGGAGAGAGACAGACCAGCCAGTAGCACGGAACTTCTGGCTGTCCAAG AAGGAATTAAAGTTCTTGAAACATTGGTTGCTCTTGGGGAAGAACAGAACAGAGTCCAGTTACTCGCTCT TCTGGTGCCCACTTTGATCTCCTACCTGCTGGACGAAAACTCATTCGCCTCAGCAAGTTCCATCTCCAAA GATCTTCATGAGTTTGCACTCCAGAACTTAATGCATATTGGACCTCTGTACCCACACGCTTTCAAGACGG TAATGGGGGCTGCTCCTGAGCTGAAAGCGCGACTAGAAACCGCTGTCCGAGCCAGCCAGGCGAGCAAAGC CAAAGCAGCCGCGAGGCAGCCTGCCCCTGCCACGCACTCCGCACCAACCATTAAGCTGAAAACAAGCTTT TTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191064
Insert Size	6096 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191064.1, NP_001177993.1
RefSeq Size	6735 bp
RefSeq ORF	6096 bp
Locus ID	362683

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