Otof (NM_001276720) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217722 representing NM_001276720 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGATCGTCCACCTCAAGACTGTTTCTGAGCTTCGAGGCAGAGCTGATCGGATCGCCAAAGTCA CTTTCCGAGGACAGTCCTTCTATTCCCGGGTCCTGGAGAACTGCGAGGATGTGGCTGACTTCGATGAGAC ATTCCGGTGGCCAGTGGCTAGCAGCATCGACAGGAATGAAGTGTTGGAGATTCAGATTTTCAACTACAGC AAAGTCTTCAGCAACAAACTAATCGGGACCTTCCGCATGGTGCTGCAGAAGGTGGTGGAGGAGAATCGGG TAGAGGTGTCTGACACGCTGATCGATGACAACAATGCTATCATCAAGACCAGCCTAAGCATGGAGGTCCG GTATCAAGCCGCAGATGGCACAGTAGGCCCTTGGGATGATGGAGACTTTCTGGGAGATGAATCCCTTCAG GAGGAAGAGAAAGACAGCCAGGAGACAGATGGTCTGCTACCTGGCTCCCGACCCAGCACCCGGACACCCG GCGAGAAGAGCTTTCGAAGAGCGGGAAGGAGTGTATTCTCGGCCATGAAACTCGGCAAGACTCGGTCCCA CAAAGAGGAGCCCCAAAGACAAGATGAGCCAGCGGTGCTGGAGATGGAGGACCTGGACCACCTAGCCATC CGGCTGGGGGATGGGCTGGATCCTGACTCAGTGTCTCTAGCCTCGGTCACTGCTCTCACCAGCAATGTCT CCAACAAACGGTCTAAGCCAGATATTAAAATGGAACCGAGTGCTGGGAGACCCATGGATTACCAGGTCAG CATCACAGTGATTGAGGCTCGGCAGCTGGTGGGTTTGAACATGGACCCCGTGGTGTGCGTGGAGGTGGGC GATGACAAAAAATACACATCCATGAAGGAGTCCACAAACTGCCCTTACTACAACGAGTACTTCGTCTTTG ACTTCCACGTCTCTCCTGATGTCATGTTTGACAAGATCATCAAGATCTCGGTTATCCATTCTAAGAACCT GCTTCGAAGCGGCACTCTGGTGGGCTCCTTCAAAATGGATGTGGGGACCGTGTACTCCCAGCCTGAACAC CAGTTCCATCACAAATGGGCTATCCTGTCAGACCCCGATGACATCTCTGCCGGGCTGAAGGGCTATGTAA AGTGTGATGTCGCCGTGGTGGGCAAGGGAGACAACATCAAGACACCCCACAAGGCCAACGAGACAGATGA GGATGACATTGAAGGGAACTTGCTGCTCCCCGAGGGCGTGCCCCCAGAACGGCAGTGGGCCCGGTTCTAC GTGAAAATTTACCGAGCAGAGGGACTGCCCCGGATGAACACAAGCCTCATGGCCAACGTAAAGAAGGCGT TCATCGGTGAGAACAAGGACCTCGTCGACCCCTACGTTCAAGTCTTCTTTGCTGGACAGAAGGGCAAAAC ATCAGTGCAGAAGAGCAGCTATGAGCCGCTGTGGAATGAGCAGGTCGTCTTTACAGATCTGTTCCCCCCA CTCTGCAAGCGCATGAAGGTGCAGATCCGAGACTCCGACAAGGTCAACGACGTGGCCATCGGCACCCACT TCATCGACCTGCGCAAGATTTCCAACGATGGAGACAAAGGCTTCCTGCCTACGCTCGGCCCAGCCTGGGT GAACATGTACGGCTCCACACGCAACTACACGCTGCTGGACGAGCACCAGGACCTGAATGAGGGCCTGGGT GAGGGGGTGTCCTTCCGTGCCCGCCTCATGTTGGGACTGGCTGTGGAGATCCTAGACACCTCCAACCCAG AGCTCACCAGCTCCACCGAGGTGCAGGTGGAGCAGGCCACACCTGTCTCAGAGAGCTGCACAGGGAGAAT GGAAGAATTTTTCCTATTTGGAGCCTTCCTGGAAGCCTCAATGATCGACCGGAAAAATGGGGACAAGCCA GTGACCTTTGAGGTGACCATAGGAAACTATGGCAATGAAGTCGATGGTACATCCCGGCCCCAGAGGCCTC GGCCCCGGAAGGAACCTGGGGATGAAGAAGAGGTAGACCTGATTCAGAACTCCAGTGACGACGAGGGTGA TGAAGCCGGGGACCTGGCCTCAGTGTCCTCCACCCCACCTATGAGGCCCCAGATCACAGACAGGAACTAT TTCCACCTGCCCTACCTAGAGCGCAAGCCCTGCATCTACATCAAGAGCTGGTGGCCTGACCAGCGGCGCC GTCTCTACAACGCAAACATCATGGACCACATTGCTGACAAGCTGGAAGAAGGTCTGAATGATGTCCAGGA GATGATCAAAACGGAGAAGTCCTACCCGGAGCGACGCCTGCGGGGTGTGCTAGAGGAACTCAGCTGTGGC TGCCACCGCTTCCTCTCCCTCTCGGACAAGGACCAGGGTCACTCGTCCCGCACCAGGCTGGATCGGGAGC GCCTCAAATCCTGTATGAGGGAGCTGGAGAGCATGGGACAACAAGCCAAGAGCCTGAGGGCTCAGGTGAA GCGACACACTGTACGGGACAAGCTGAGGTTATGCCAGAACTTCCTGCAGAAGCTACGCTTCCTAGCTGAT GAGCCCCAGCACAGCATCCCCGATGTGTTCATTTGGATGATGAGTAATAACAAACGTATCGCCTACGCCC GCGTGCCTTCCAAAGACCTTTTGTTCTCCATTGTGGAGGAGGAATTGGGCAAGGACTGCGCCAAGGTCAA GACCCTCTTCCTGAAGCTGCCAGGAAAGAGGGGCTTCGGCTCGGCAGGCTGGACAGTACAGGCCAAGCTG GAACTCTACCTGTGGCTGGGCCTCAGCAAGCAGAGAAAGGACTTCCTGTGTGGCCTGCCTTGTGGCTTCG AGGAGGTCAAGGCAGCCCAAGGCCTGGGTCTGCATTCCTTTCCGCCCATCAGCCTTGTCTACACCAAGAA GCAAACCTTCCAGCTCCGAGCCCACATGTATCAGGCCCGAAGCCTCTTCGCCGCTGACAGCACTGGGCTC TCTGATCCTTTTGCCCGAGTCTTCTTCATCAACCAGAGCCAGTGCACGGAGGTCCTAAACGAGACACTGT GTCCTACTTGGGACCAAATGCTGGTATTTGACAACCTGGAGCTGTACGGTGAAGCTCATGAGTTACGAGA TGATCCCCCCATCATTGTCATTGAAATCTACGACCAGGACAGCATGGGCAAAGCTGACTTCATGGGCCGG ACCTTTGCCAAGCCCCTGGTGAAGATGGCAGACGAGGCATATTGCCCGCCTCGCTTCCCGCCCCAGCTTG AGTACTACCAGATCTACCGAGGCAACGCCACGGCCGGAGACCTACTGGCTGCCTTTGAGCTGCTGCAGAT TGGACCATCGGGGAAGGCTGACCTGCCACCCATCAATGGCCCAGTGGACATGGACAGAGGGCCCATCATG CCTGTGCCTGTGGGAATCCGGCCCGTGCTCAGCAAGTACAGAGTGGAGGTGCTGTTCTGGGGCCTGAGGG ACTTAAAGAGGGTGAACCTGGCCCAGGTGGACCGGCCACGGGTGGACATCGAGTGTGCGGGAAAAGGGGT ACAATCGTCCCTGATCCACAATTATAAGAAGAACCCCAACTTCAACACCCTGGTCAAGTGGTTTGAAGTG GACCTCCCAGAGAATGAGCTCCTGCACCCACCCTTGAACATCCGGGTGGTGGACTGCCGGGCCTTTGGAC GATACACCCTGGTGGGCTCCCATGCAGTCAGCTCACTACGACGCTTCATCTACCGACCCCCGGACCGCTC GGCCGCCAACTGGAACACCACAGTCAGGCTTCTCCGGGGCTACCACATGCTGTGCAATGGGGGCCCCTCT TCTTGCCCCACAGGGGAGGTTGTAGTGAGCATGGAACCTGAAGTGCCAGTTAAGAAGCTGGAGACCCTGG TGAAGCTGGATGCGACTTCTGATGCTGTGGTCAAGGTGGATGTGGCTGAAGATGAGAAGGAAAGGAAGAA GAAGAAAAAGAAAGGCCCATCAGAGGAGGCAGAGGAGGAGGAACCCGATGAGAGCATGCTGGATTGGTGG TCCAAGTACTTTGCCTCCATTGACACAATGAAGGAGCAACTTCGACAACATGAGACCTCTGGGATTGATT TGGAAGAGAAGGAAGAAATGGATAGCACTGAGGGCCTGAAGGGGCCAGTGAAGAACAAGGAGAAGTCCAG AGCTGCAAAGGAGGAAAAAAAGAAGAAAAATCAGAACCCTGGCCCTGGCCAGGGATCGGAGGCTCCTGAG AAGAAGAAAGCCAAGATCGACGAGCTTAAGGTGTATCCCAAGGAGCTAGAGTCGGAGTTTGACAACTTTG AGGACTGGCTGCACACGTTCAACCTGTTACGGGGCAAGACTGGGGATGATGAGGATGGCTCCACAGAGGA AGAACGCATAGTAGGCCGATTCAAGGGTTCCCTCTGTGTGTACAAAGTGCCACTCCCGGAGGATGTGTCT CGTGAAGCTGGCTACGATCCCACCTACGGAATGTTCCAGGGCATCCCAAGCAATGACCCCATTAATGTGC TGGTCCGAATCTATGTGGTCCGGGCCACAGACCTGCACCCGGCTGACATCAATGGCAAAGCCGACCCCTA TATCGCCATCAAGTTAGGCAAGACTGACATCCGAGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCT GTCTTTGGAAAGTCCTTTGACATTGAGGCCTCCTTCCCCATGGAGTCCATGCTGACAGTGGCTGTGTACG ACTGGGATCTGGTGGGCACTGATGACCTCATCGGAGAAACCAAGATCGACCTGGAAAACCGCTTCTACAG CAAGCACCGTGCTACCTGCGGCATCGCGCAGACCTATTCCATACATGGCTATAATATCTGGAGAGACCCC ATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGAGGGCAAAGTGGACGGCCCCCACTTTGGTCCCC ATGGGAGAGTGAAGGTCGCCAACCGTGTCTTCACAGGGCCTTCAGAGATAGAGGATGAGAATGGTCAGAG GAAGCCCACAGATGAGCACGTGGCACTGTCTGCTCTGAGACACTGGGAGGACATCCCCCGGGTGGGCTGC CGCCTTGTACCGGAGCACGTGGAGACCAGGCCGTTGCTCAACCCTGACAAGCCAGGCATTGAGCAGGGCC GCCTGGAGCTGTGGGTGGATATGTTCCCCATGGATATGCCGGCCCCTGGAACACCTCTGGATATCTCCCC CAGGAAACCCAAGAAGTATGAGCTGCGCGTCATCGTGTGGAACACAGATGAGGTGGTCCTGGAAGACGAC GATTTCTTCACGGGAGAGAAGTCCAGTGATATTTTTGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACA AACAGGACACAGACGTACATTATCACTCCCTCACGGGTGAGGGCAACTTCAACTGGCGATACCTGTTCCC CTTCGACTACCTGGCGGCCGAAGAGAAGATCGTTATGTCCAAGAAGGAGTCCATGTTCTCCTGGGATGAG ACGGAGTACAAGATACCTGCGCGGCTCACCCTGCAGATCTGGGACGCTGACCACTTCTCAGCCGATGACT TCCTGGGGGCCATCGAGCTGGACCTGAACCGGTTCCCGAGGGGCGCCAAGACAGCCAAGCAGTGCACCAT GGAGATGGCCACCGGGGAGGTGGATGTGCCCCTGGTTTCCATCTTTAAACAGAAACGTGTCAAAGGCTGG TGGCCCCTCCTGGCCCGCAATGAGAATGATGAATTTGAACTCACGGGCAAAGTAGAGGCAGAGTTACACC TCCTCACGGCAGAGGAGGCGGAGAAGAACCCGGTGGGCCTGGCTCGCAATGAACCTGACCCTCTAGAAAA ACCCAATCGGCCCGACACAAGCTTCATCTGGTTCTTGAACCCTCTCAAGTCTGCCCGCTACTTCCTGTGG CATACCTACCGCTGGCTGCTCCTCAAACTCCTGCTGCTCCTCCTCCTGCTGCTGCTGCTCGCCCTGTTTC TCTACTCTCTGCCTGGCTACCTGGCCAAGAAGATCCTTGGGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276720
Insert Size	5997 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276720.1, NP_001263649.1
RefSeq Size	7118 bp
RefSeq ORF	5997 bp
Locus ID	84573
Gene Summary	human homolog is associated with an autosomal recessive form of prelingual sensorineural deafness, DFNB9 [RGD, Feb 2006]

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