Sbf1 (NM_001271173) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217707 representing NM_001271173 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG AAGTGATGTGCACTGAAGATGCCACAGAGAAGGAGGAAGAGGCACATGGAGGAGGCCAAGCACAGCTGTC ATCCACAGCCCCAGCCCAACCTGGCCAGCTTTTTGCTCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC CATGCTGACGTGTTCAGGAATAGCCTTGGTCTCATCTATACCATCCATGTGGAAGGCCTGAACGTGAACC TGGAGAATGTGATTGGGAATCTGCTCACGTGCACCGTCCCCTTGGCAGGCGGGTCTCAGAGAACCATCTC TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACCCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT GTAGCCCTGCTCTTCCGCCAGCTGGGCATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTCACTGAGC ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCCGATGCTTGCAGGGGCCTCTTGGCACTGCT GTTTCCTCTCAGATACAGCTTCACATATGTGCCCATCCTGCCGGCACAGCTGCTCGAAGTCCTTAGTACA CCTACACCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGACGTGATTG TTGCCGATCTTGATGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT ACAAAGCCAGACTCACAATGTTCTGAGCATGGTCCTGGATCCAGAGCTGGAGTTGGCTGACCTTGCCTTT CCACCACCTACAACATCTGCTTCCTCACTGAAAATGCAGGACAAGGAGCTCCGCGCTGTCTTTCTGCGGC TCTTTGCTCAGCTCCTGCAGGGTTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCCGAGCCTGTCAT TCGCTTCCATAAGGCAGCGTTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTGTTG GAGGGCATGGCCTTTGCAGGCTTTGTGTCAGAGCGTGGGGTCCCCTACCGTTCTACAGACCTGTTTGATG AGTTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAACCATCCCCACCGCGTCCTGCGTCACGT CCAAGAACTAGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCAATGCATAAAGTGCAG AGGCCAGGAGAGGCCAGCCACCTGCGGCGGACCCATCGGCCATTCCCCCGGCTAGATGAGGGCACAGTTC AGTGGATTGTGGACCAGGCTGCAGCCAAGATGCAGGGTGCACCTCCAGCTGTGAAGGCTGAAAGGAGGAC CACTGTCCCTTCAGGGCCCCCCATGACGGCCATCCTAGAGCGGTGCAGTGGGCCTCACATCAACAGTGCC CGTCGCCTGGAGGTGGTGCGCAACTGCATCTCCTACGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT TGCTTCCAGCTGTTCTCAGGGCCCTGAAGGGGCGAGCTGCCCGCCGCTGCCTCGCCCATGAGCTTCACCT GCACGTACAGCAGAACCGTGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC TGCCTGCAGGATTGCACTTCTCTGGATGAGCACGGCATTGCGGCTGCACTGCTGCCTTTGGTCACAGCCT TCTGCAGGAAGCTGAGCCCAGGGGTAACGCAGTTTGCATATAGCTGTGTTCAGGAGCATGTAGTGTGGAG CACACCACAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGGGCCCTCTACCTGGAG CCTTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC TGGATGTGGCTTCAGAGCAGAGGCGCCTATGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAACTGGTACA GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGCGGCTTTGAAGACGC AGAGACATGCGATGTGGCTGGGGCTGTGGTCCGCTTCATAAACCGCTTTGTGGACAAGGTCTGCACAGAG AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG AGACCCTGGAGGCTGTACACCGTGAGAGCAAGAGGCTGCCCCCAATACAGAAGCCCAAGCTGCTGAGGCC ACGCCTATTGCCTGGTGAAGAGTGTGTCCTCGATGGCCTTCGGGTGTACCTGCTGCCAGATGGGCGTGAG GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTTCCAGCTGAGGGTGCTGTCTTCCTTACCACAT ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTTGTCCGCTCCTTCCC CGTGGCTGCGTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGACGGG CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG CTGAGCTCTTCCGAAAGCAGCTCCACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC ACTTGGCTCAGCTCTCACACCTGGCAGGCCGCCACGAGTTACCAAGGACAAGGGTCCTTCATTCAGAACC CTGTCCAGGAACCTGGTGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAATATA ACCCCCCTGGCTGGGAACATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATATCAGTGTCAGA GGAGCTGGAGCCCAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGT CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGTCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG ATTGCTGATTGTTCCCCAGAGCATCCAGGACAATGCCCTGCAACGCGTGTCCCGTTGCTACCGCCAGAAC CGTTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTGTTGCTACGCTCTGGGGGCCTGCATG GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCTGACTCCAG CAGCCTAGAGCAAGAGAAGTACCTGCAGGCTGTGGTTAGCTCCATGCCACGTTATGCTGACTCATCAGGA CGAAACACACTTAGTAGCTTCTCCTCAGCCCACATGGGTGGTCACGTGCCCAGTCCCCGAGCCAGGGTCA CCACGCTGTCCAACCCTTTGGCGGCCTCGGCCTCCAGATGGACTGCGTCCCGAGGTAAGTGGAGCAGTGT CCGAGCCAGTGGTCGAAGCAGTGGGCTTGGTGCTGATGTGGGCTCTCGGCTGGCTGGCAGAGACCTCCTC AGTACTCCCCACACCAACGGAGCGCCACCTGATTCTGGTTTTCTGCGGCCGCAGCGTGCAGCCCTCTACA TCATTGGTGACAAAGCTCAGCTCAAGGGTGTGCGCCCAGACCCTTTGCAACAGTGGGAGCTGGTTCCTAT TGAAGTATTTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAATTGTTGAAGGCCTGTGTGCCTGGTTGC CCTGCCACTGAGCCCAGCCCAGCCTCCTTTCTGCGGTCACTGGAGGACTCAGAGTGGCTGATCCAGATAC ACAAGCTGCTGCAGATATCGGTGCTGGTGGTGGAGCTGCTAGACTCAGGCTCCTCTGTCCTTGTGAGCCT GGAGGATGGCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTCCTCTCAGACCCCTTCTACCGC ACTCTGGAAGGCTTCCGCCTGCTAGTGGAAAAGGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGTG GGGCCCACACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACA CCAGGTCCATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTATCAC CACACATCCCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGACTGCTGT ATGAGGAGAAGGGTGAGCGCAGAGGCCAGTTGGCTTGCAAGTCTGTGTGGGAATATGTAGACCGGCTAAG CAAGAGGACCCCCATGTTCTACAACTACACATATGCACCTGAGGACACAGAGGTGCTGCGGCCCTACAGC AATGTGTCCAACCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACT GGGAGCTGGCCCAGGGGCCTCCTGAGCCTCCAGAAGAGGAGCGTCCTGATGGAGGTGCTCCCCAGAGCAG GCGCCGTGTGGTTTGGCCGTGCTATGACAGCCGCCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTG GAGGAGCTGCAGCGATTGGAGACAGAGCTGGGACGGCCTTCTGAACGCTGGAAAGAGACCTGGGACCGAG TGAAAGCTGCTCAGCGCCTTGAAGGCCGGCAAGATGGACGTGGTACCCCTAGCTCATTACTGGTGTCAGC TGTGCCCCACCACCGCCGCTCGCTAGGGGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTC AGCCTGGACAGTGACCAGAGCAGTGGCTCAACCACATCCAGCTCTCGTCAGGCTGCCCGACGGAGCACCA GCACGCTGTATAGCCAATTCCAGACAGCGGAGAGTGAGAACAGGTCCTATGAGGGCACCCTGTACAAGAA GGGAGCCTTCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTGGACAAGACCAAGCACCAGCTGCGTTAC TATGACCACCGAGTGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTG GCACACCCACCATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTCGATGTGAAGACAACACGTCG TGTTTACAACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTG TCGGATGCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271173
Insert Size	5682 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271173.1, NP_001258102.1
RefSeq Size	6155 bp
RefSeq ORF	5682 bp
Locus ID	300147

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