Gbf1 (NM_001191634) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1307160
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217702 representing NM_001191634 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGATAAGAATATTTACATCATTCAAGGAGAAATTAACATTGTTGTTGGTGCCATCAAACGAAATG CCCGATGGAGCACCCATATACCACTGGATGAAGAGCGGGATCCCCTTTTGCACAGTTTCAGCCATCTAAA GGAGGTCTTAAACAGTGGGACAGAACTCTCAGAGATTGAGCCAAATGTATTCCTTCGTCCATTTCTGGAA GTCATTCGCTCTGAAGACACCACTGGTCCTATCACCGGCCTGGCACTCACCTCTGTCAACAAGTTCCTGT CCTGTGCACTCATAGATCCAACTCATGAGGGCACGGCAGAGGGCATGGAGAACATGGCAGATGCTGTCAC TCATGCCCGTTTTGTGGGCACAGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAGGTTCTC CGGACTTTGTTGCTAACCCCAGTGGGTACCCACTTAACAAATGAATCTGTATGTGAGATTATGCAGTCTT GCTTCCGGATTTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCCGCAGAGCACACTCTCGTAGA CATGGTGCAGCTGCTCTTCACAAGGTTACCGCAGTTTAAAGAAGAACCCAAGAGTTACGTGGGAACCAAC TTGAAGAAGCTGAAAATGAGAGCAGGAGGCATGAGTGACTCATCCAAGTGGAAGAAACAGAAGCGATCCC CTCGACCTCCACGTCATACAACCAAAGTCATGCCAGGTTCAGAGCTGCCCACCCCAAATGGAGCCGCCTT ATCCTCTAACCTTACTGGTGGCATGCCCTTCATTGAGGCACCCACATCCATCTCCTCTGCAAGTTCAGAA GCTGCCTCCACAGTGGTCAGTCCCTGTACAGATAGTGGCCTGGAATTATCCTCCCAGGCTACCTCCAAGG AGGACCTCACCGACCTGGAGCAAGCTGGCTCCCCAAGGGAGATCACCACCACAGAGCCTGGGAGCACTGA GATAGGAGTTTCTGATCAGCTTGACCCTCAGGAAGGGGCCCATGTAGAAAAGGCCCAGTCAGCATCGGTG GAATCTATCCCTGAAGTGCTAGAGGAGTGCACATCCCCTACTGACCATTCTGACTCTGCCTCTGTCCATG ACATGGATTATGTCAATCCTCGGGGCGTGCGCTTTACCCAGTCCTCCCAGAAAGAAGGCACAGCTTTGGT TCCTTATGGTCTTCCTTGCATCCGTGAGCTCTTCCGCTTCCTTATCTCCCTCACAAATCCACATGACCGC CATAACTCTGAGGTTATGATCCACATGGGACTTCATTTGCTGACAGTAGCTCTTGAGTCAGCCCCTGTGG CCCAGTGCCAGACCCTCTTGGGTCTCATCAAGGATGAGATGTGCCGACACTTATTCCAGCTACTCAGCGT AGAGCGGCTGAACCTTTACGCTGCTTCCCTACGAGTGTGCTTTTTGCTCTTTGAGAGCATGCGGGAACAC CTCAAGTTCCAGTTGGAGATGTACATCAAAAAGCTCATGGAGATTATCACTGTTGAAAACCCCAAGATGC CTTATGAGATGAAGGAGATGGCGCTGGAGGCCATTGTGCAGCTCTGGCGCATCCCCAGCTTCGTCACGGA GCTCTACATCAACTATGACTGTGACTACTACTGCTCCAACCTCTTCGAAGACCTCACTAAGCTGCTGTCC AAGAATGCCTTTCCTGTGTCTGGCCAGCTTTATACAACACACCTGCTGTCTCTTGATGCCCTATTGACAG TTATTGACAGCACTGAGGCTCATTGTCAGGCCAAAGTCCTCAACACCCTCAACCAGCAAGAGAAGAAAGA GACAGCCAGACCTGGCTTTGAGGCAGTGGATGGCAGCCCAGACACTTACAAGTCTGAGAGAGCAGCCAGT GATGGGAAAGCCACAGGAGTGCCCTCAGATGCTCCAGGCCTTCATTTTTCAAGTGGAGGGTGGTTGTCAA CAGAACATGGAAAGCCAGGGTGCAGTGATCTGGAGGAAGCTGGTGACTCTGGGGTTGACAAAAAGACCAC CAGGAAGCCACCTCGATTTTCCTGTCTTCTGCCAGATCCACGGGAACTAATTGAAATTAAAAACAAAAAA AAGCTGCTGATCACTGGTACAGAGCAGTTCAACCAAAAACCAAAGAAGGGGATCCAGTTTCTGCAGGAGA AAGGCCTCCTCACCATCCCAATGGATAACACAGAGGTAGCCCAGTGGCTCCGAGAAAACCCTCGGCTAGA CAAAAAAATGATTGGAGAGTTTGTGAGTGACCGCAAAAACATGGACCTGTTGGAGAGTTTTGTGAGCACC TTCAGCTTCCAGGGTCTGAGGCTTGATGAGGCTCTCCGCCTCTACCTAGAAGCCTTCCGTCTGCCTGGGG AAGCGCCTGTTATTCACAGGCTGCTGGAGGTGTTCACTGAGCACTGGAGGAGTTGTAATGGCTCCCCATT TGCTAATAGCGATGCCTGCTTTGCCCTGGCCTATGCTGTTATCATGCTTAACACTGACCAACATAACCAC AATGTTCGCAAGCAAAATGCACCCATGACCCTGGAGGAGTTCCGCAAAAACCTGAAAGGTGTGAATGGAG GCAAGGACTTTGAGCAAGACATCCTGGAAGACATGTACCATGCCATCAAGAATGAGGAAATTGTGATGCC TGAGGAACAAACAGGTCTGGTTCGTGAGAATTATGTGTGGAGTGTGCTGCTTCATCGAGGTGCTACCCCT GAGGGCATCTTCCTTCGTGTACCCCCTGGCAGCTATGATCTTGACCTCTTCACTATGACCTGGGGCCCCA CTATTGCTGCTCTCTCTTATGTGTTCGATAAAAGCCTCGAGGAGACCATCATCCAGAAAGCCATCTCAGG CTTCAGGAAGTGTGCCATGATCTCCGCCCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCT CTGTGCAAATTCACAGCTCTAAGTAGTGAGTCTATTGAGAACCTGCCCAGTGTATTCGGAAGCAACCCTA AAGCCCACATTGCAGCCAAGACAGTATTCCATTTGGCCCATCGTCATGGTGACATCCTTCGGGAGGGCTG GAAGAATATCATGGAGGCCATGCTGCAGCTCTTCCGAGCCCAACTTTTACCGAAGGCTATGGTAGAGGTA GAAGATTTTGTGGATCCCAATGGTAAGATCTCTCTACAGCGGGAGGAGACACCATCAAACCGAGGAGAGT CAACAGTCCTTAGTTTTGTAAGCTGGCTGACACTGAGTGGTCCTGAGCAGTCCAGTGTCCGGGGCCCATC GACAGAGAACCAAGAGGCCAAGCGAGTAGCCTTGGACTGCATCAAGCAATGTGACCCAGAAAAGATGATC ACAGAAAGCAAGTTCCTCCAGCTGGAATCTCTGCAGGAGCTCATGAAGGCTCTGGTCTCGGTGACACCAG ATGAAGAGACATATGATGAAGAAGATGCTGCTTTCTGTCTGGAGATGCTGCTAAGGATTGTGTTAGAAAA CAGGGACCGTGTGGGCTGTGTATGGCAGACTGTTCGAGATCATCTATACCACCTGTGTGTTCAGGCACAA GATTTCTGCTTCCTTGTGGAGCGAGCAGTGGTAGGGCTGCTACGCCTAGCCATTCGGCTACTCCGAAGAG AAGAGATTAGTGGCCAGGTCCTGCTCTCTCTGCGCATCTTGTTACTGATGAAGCCCAGCGTGCTGTCCAG AGTCAGCCACCAGGTCGCTTATGGGCTGCATGAACTCCTCAAGACCAATGCTGCCAACATCCACTCGGGT GATGACTGGGCCACTCTCTTCACATTGCTGGAGTGCATTGGCTCAGGCGTGAAGCCTCCAGATGCTCTAC AAGCCACAGCCAGGGCTGATGCTCCTGATGCTGGAGCACAATCCGACAGTGAGCTCCCATCCTACCATCA AAATGATGTCAGCCTAGACCGAGGGTACACTTCCGACTCAGAAGTCTACACTGACCATGGCAGGCCTGGC AAGATGCACCGATCAGCCACAGACGCTGACATGGCCAACAGTGGTTGGTTAGTGGTGGGGAAGGATGACA TTGATAACTCCAAACCAGCAGCAGGACTCAGCAGGCCAGGCCCTTCGCCCTTGGTCAATCAGTACAGCCT TACAGTGGGCCTGGACCTGGGACCACATGACACTAAGTCCTTGCTTAAGTGTGTGGAGTCTCTGTCCTTC ATTGTTCGTGATGCTGCTCACATCACCCCTGACAACTTTGAACTCTGCGTCAAGACCCTCCGCATCTTTG TAGAAGCCAGTCTGAATGGTGGGTGCAAGTCCCAGGATAAACGTGGCAAGAGTCATAAATATGACAGCAA AGGGAACCGGTTCAAGAAAAAGCCCAAGGAGGGCTCGATGCTTCGGCGGCCCCGGGCATCCAGCCAGCAT GCCACTCGGGCTGGACACAGTGATGAAGAGGAGGACGAAGGCGTGCCTGCCAGCTACCATACGGTGTCTT TACAGGTCAGTCAGGACTTGCTAGACCTGATGCACACCCTGCACACACGGGCAGCCTCTATCTACAGTTC ATGGGCAGAGGAGCAACGCCACCTGGAGACAGGTGGCCGGAAGATTGAAGCTGACTCACGTACCCTCTGG GCCCACTGCTGGTGCCCTTTATTACAAGGTATCGCTTGCTTATGCTGTGACGCCCGGCGCCAGGTGCGGA TGCAGGCCCTGACATATCTCCAGCGAGCCCTTCTGGTACATGACCTGCAAAAGCTAGATGCCCTGGAATG GGAGTCCTGTTTTAACAAGGTGCTGTTTCCGCTACTGACCAAGCTTTTAGAAAATATCAGCCCTGCGGAT GTGGGCGGGATGGAGGAGACCCGGATGCGGGCTTCCACACTGCTCTCAAAGGTCTTCCTGCAGCACCTGT CCCCACTGCTGTCCCTGTCCACCTTTGCTGCCCTGTGGCTCACCATCCTGGACTTCATGGACAAGTACAT GCACGCAGGTTCCAGTGACTTGCTGTCAGAAGCTGTCCCTGAGTCTCTGAAGAACATGCTCCTTGTGATG GACACAGCAGAGATTTTCCACAGTGCAGATGCAAGGGGAGGCAGCCCCTCTGCTCTCTGGGAGATCACCT GGGAGCGCATCGATTGCTTCTTACCACACCTACGTGATGAACTCTTCAAGCAGACTGTCATCCAGGACCC CATGCCCACAGAGCCTCACAGTCAAAAACCTCTGGCTTCTACTCACCTGACGCCTGCTGCTGGTGACCCC AGGATGCCTGGCCATCCACCTCCTCCAGAGATACCCTCAGAAGTGGGAGCTTGTGACCCGGAGAAGCCCG AGAGCACCAGAGCTGCCAGCAGCAGCTCCCCTGGATCGCCGGTGGCCTCTAGCCCCAGCAAACAGAGTCC TGCCCCAGAGGGACCTCCCCCACTGGCCCAGCCCCCACTAATCCTACAGCCCTTGACCTCCCCACTGCAG GTGGGGGTACCACCCATGACTTTGCCCATCATCCTCAACCCTGCACTCATTGAGGCCACCTCTCCAGTGC CTCTCTTAGCCACGCCACGCCCTACAGACCCCATCCCCACCTCTGAGGTCAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191634
Insert Size	5586 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191634.1, NP_001178563.1
RefSeq Size	6369 bp
RefSeq ORF	5586 bp
Locus ID	309451

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