Alpk3 (NM_001191895) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1309860
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217679 representing NM_001191895 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCGGAGGGCCTTGGGCCGGGGCTGGGGCCTGGGTGGTCGAGCAGGAACCGGTGGAGATGGCG AGGACGACGGGCCGGTGTGGACTCCTGGCCCAGCCAGTCGCAGCTACTTGCTCAGCGTGCGGCCGGAGGC TAGCTTATCAAGCAACCGGTTGTCTCACCCCAGCTCTGGAAGGAGCACCTTCTGCTCCATCATTGCTCAG CTTACAGAGGAGACCCAGCCGCTGTTTGAGACCACACTCAAGTCCCGAGCTGTGTCCGAAGACAGTGATG TCAGGTTCACCTGCATTGTCACAGGATACCCAGAGCCAGAGGTGACCTGGTACAAGGATGACATAGAACT GGACCGTTACTGTGGCTTGCCAAAATACGAGATCACTCACCAAGGCAACCGGCACACCCTGCAGCTGTAC AGGTGTCGGGAAGAAGATGCTGCCATCTACCAGGCCTCTGCCCGGAACACCAAGGGCATCGTGTCTTGCT CAGGGGTCCTAGAGGTGGGCACTATGACGGAGTACAAGATTCACCAGCGCTGGTTCGCCAAGTTGAAGCG TAAGGCTGCGGCCAAGATGAGGGAGATTGAGCAGAGCTGGAAGCACGGGAAAGAGGCTTCAGGGGAGGCT GACACACTTCGCAAGATCAGCCCGGACCGCTTCCAAAGAAAGCGCCGACTGAGTGGAGCGGAAGAGGCAG TCCTCCCGGCGCCACTCAGGGGAACGGAGGAAGGGCCCTCAGCAGCTTGGCAGGAAGGAGAGGCTGAGTC CACTCAGCACCCAGGTTTGGGGATGATCAACAGTTTCGCTCCTGGTGAGGCACCCACCAATGGGGAGCCT GCCCCAGAGAACGGGGAGGACGGAGAGCACGGCCTGCTGACCTACATCTGTGAGGTCATGGAACTGGGGC CTCAGAACACCCCTCCAAAGGAGTCTGGGGCTAAGAAGAAAAGGAAGGATGAGGAATCTAAACCAGGAGG GCAGAAACCAGAGTTAGGGAAGGCAGAAGGGAGCCAGTGCTCTTCAGAAAATGGCATCCCTAGCTCAGAC AAACTCAATTCCAGTAGAAGGGAGAAGAAGTCCAGGGATGTGCAGCCAACTCAGAACCAGACCAGAGACA GGGTAGCACGGGGGCCTGGGCCTTCTGGGATAGAAAGCACCAGGAAGACAGCCTCTGTTCTGGGTACTCA AGACAAGGTCCAGGATGTCCCGGCCCTGGCCCCGGCCCCCGCCCCGGCCCCCGCTCCTGTCCCAGTCCCT GCACCCGCCCCTGTTCCAAGCCTCAGCTCAGAGCAGGTGTATTTCTCGCTGAAGGACATGTTCATGGAGA GCACCCGGGCAGGCAGGTCCCAGGAAGAGGAAAAATCTCCACCTCCAAGTACCAGAGTAGCTGGAGAAAG TCCTTCAGGGAAGGTACCTGTCAAGGCTAGAGGGGAGAAGGTACCTATGGTCTCTGGCCAGCCCACGTCT TCCACGGTGCCCCCGCCTATTAAACCTTTGAACAGGAAGAGATTTGCCCCTCCTAAATCCAAAGTGGAGT CAACTACCTCCTCTTCCTCAAGTCAGACTCCAGAATCTGTGGCCCAGAGCTTAGGGAAGGCCCCACCGCC AGCCTCTGCCCAGGTCCCAACACCCCCTGCCCGACGGAGACATGGCACTCGAGAGAGCCCCTTGCAAGGA CAAACAAGCCACAAGACTCCAGGAGAGGCTCTGGAGTCCCCAGCAACCATGGCCCCTGCCACGTCTGCCA GCAGCAGTTCCGATACCATCTCTGTTGGTCACAGCACCTCTGGGAATCAAGGTGTCACGGAGCCCATGGA TACAGAAACTCAGGAAGAGGGGAGGACACTTGCCAATGGGAGAACCAGTGGCAGGCAGAAAACACAGACA GACGGAAAGTTGCAAGTGGATGGGAGGACTCAGGGAGACGAAGCACAAACTTTGCAGAGGACACAGACAA ATGAGAAGGCACCGATGAACGTTGTAACACAAGGAAGCGAAAGGCCAGAGTCAGACAGGAGTTCACAGAA GGATTTGGTGACACAGAGAAGGGTGGATACACAGGTAGGACAGACACAGGCAGGTGAGAGGTGGCAGCAA GACCCGAGAGACGCAAGGATACAGGAGGAAAAGGAGACACAGTCAGTGGGCAGCATTCCCACAGCTTTGG AAACCCAACCAGAACAACCGTCTGTGGTCAGTCTCAGCTCAGTACCAGGAGCCCTCAAACTCTCGCCGTC AGAGTGCCCTACAGAGCCTCAGGCTACAGAATGTTCTGAGAGGAGCACAGAAGCATCTTACATCCAAGAA AAGTCAGGCTTGATGCTTGGATCTGAAGAGGCAGACTTCAGAAGCCATGAGAACACTCTGCTAGGGCCCC CATCAGGGAACCATACCTACTCAACACAGTTGCCCACTGAGGGCAACTCAGAGCATCTGGTAGGAGAAAC ACACCAAAGGCCAGAGTGGTTAGGTGAGGCCAAGCAGGAGGACAGCCTGCTTCAGAGTCCCAAGAAGGAG CAGCTCCAGGAGACGCTGTATGTGGATCTCGCTATTGGACATTCAGCGGACTTGAGTCAGGAGGTGCCCA GTCTGCCTTCTCTTCCTGGAACTGACCTAACCAATAGTCTGCAGGAGGGGCTACCCAGCACCACAGCTTC TCAGCACACAAACGCTGATGTCCCCCTCCCCTCCCCGGACCAGGACCTGCCCAGTTCTGCTCCCACTCTG CAACTGGGACCAGGGTCCCCGACTCAGAGTCACCCACCAGAAGTCATGGCTACTAGCAGTGAGGGAGCCT GTGCCAAGGAGCCAGATGTGGACGGGAGGTCCTCAGGTACCCGGAGCTGTGACCCTGGCCTTATAGACTC CCTGAAGAACTACTTGCTCCTGCTGCTAAAGCTATCCAGTCCAGAGGCAGGCGAAGCCAGGGCAGAGTCC CAGGAAGTGCCTGCCACCGGAGGCTTAACTTCCTCCTCTGCTCTGGTCCCCACCGTGGAAGTGGCTGGCT TGAGTCCCAGGACGTCCAGGCGCATCCTGGACCGTGTGGAGAACAACCACTTGGTGCAGAGCGCACAGAC CCTGCTGCTGAGCCCCTGCACCTCCCGCCGCCTCACTGGCCTCCTGGACCGTGAGGTTCAGGCTGGCCAG CAGGCCCTGGCTGCCGCCCAGTGCTCCCGGGGCCCATGCCCTAGCCCCAGCCCCCTCACCATCCCTGCCA TTGTGGTGGGTGAGGAAGGATCTGCTACTGGTGAGGGAGAGGATTCCAGGGAGAGGACCTCCCAGGAAAG TGACAAGGCTGGACTGCTAGGGGAGGTGGACGGACAAACAGTAGAGAGCAGAACCCAGGAGCCCTGCCAA GAAGAAGCAATCCCAGGGGAGGCTTTGACAGGTCTCCCTGCAGCTACACCTGAGGAACTGGCTCTAGGGG CCCGGAGGAAGAGGTTCCTCCCTAAGGTGAGAGCAGGGTCAGACGGTGAGGTGAACAAGGCTGAAGAAAG AGAGAGTCCCACAGTCTCCCCCCGGGGACCCAGGAAGGGTCTGGCACCTGGGTCACCCGGGACTCCAGGG CGGGAGAGACGGTCCCCTACCCAGGCCCGGAAGGCCAGCATGTTAGAGGTGCCTGGGACAGAAGAAGAGC CTGCATCGGGAGACTTGGTCACCAGACCCAAGGCCAGTGGTCTGGAATCAGAGCCTGCGGTGGATGAAGG CAAGCAGGAAGCACTGGCTAAGCCAAGGAAAGCTAAAGACCTGTTAAAAGCCCCACAGGTGATCCGGAAA ATTCGGGTAGAACAGTTTCCAGATTCTTCTGGTAGTCTGAAGCTTTGGTGTCAGTTTTTCAATATCGTTA GCGACTCAGTCTTGACATGGGCGAAGGATCAGCATCTAGTGGGCGAAGTGAGCAGGAGTGCAGGGGACGA GGGGCCAGCGGCCTTGGCCATTGTGCAGGCGTCACCTACGGACTGTGGCCTGTATCGCTGTACCATCCAA AACGAGCATGGCTCCGCTTCCACTGACTTCCGCCTCAGCCCGGAAGTATTGTCTGGCTTCATCTCCAGAG AGGAAGGTGAAGTTGGAGAAGAGATTGAGATGACCCCCATGGTATTTGCTAAGGGTCTGGCTGACTCTGG CTGCTGGGGGGACAAGCTCTTTGGGCGATTGGTGAGCGAGGAGCTTCGAGGGGGTGGACATGGCCTTCAG AAGGCTTCGCGGGCCAAGGTCATCTATGGGCTGGAGCCCATCTTCGAATCTGGTCGCACGTGCATCATCA AGGTATCCAGCCTGCTCGTGTTCGGACCCAGCAGTGAGACTTCTCTTCTGGGCAGAAACTATGACGTCAC TATCCAGGGATGCAAGATCCAGAACATGAGTCGAGAGTACTGCAAAATCTTTGCAGCTGAAACCCGGGCA GCCTCTGGCTTCGGGGAGGTTCCTGAGATCATCCCACTTTACTTGATCTATCGGCCTGCAAACAATATAC CGTATGCTACCCTGGAGGAAGATCTGGGCAAGCCCCTGCAGACTTACTGTTCCAGGCAGTGGAGCTGTGC TGGGGCCCCAGCAGCATCCAGCAGCTTGGAGCCTGTGCAGAAATGCCAAACCTTCCAGCACTGGCTGTAT CAGTGGACGAACGGCAGCTTCCTTGTCACAGATCTGGCAGGGGCTGACTGGAAGATGACTGATGTAGAGA TCGCTACCAAACTCCGAGGATACCAAGGCCTCAAGGAGAGCTGTTTTCCTGCCCTGCTGGACCAGTTTGC CTCTTCCCACCAGTGCAACACCTACTGTGAGATGCTGGGGCTGAAACCCCTCAAGGGCCCTGAGGGTGCC CACCCTCAAACCAAGGCCAAAGGCTCCAAAAGTCCATCTGCTGGCAGAAAAGGTTCCCAGCTGAGTCCTC AGCCCCAGAAGAAAGGTCTTCCCAGTCCTCAGGGCTCCAGGAAGAGCGCTCCAAGCTCCAGAGCCACGCC TCAGGCCTCCCAGGAGGCCACTCTTCAGTTACTGGGACAGCCCCCTGCCCAAGAGGGGAGCTCTAAGGCC CAGGGCATGCGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191895
Insert Size	5055 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191895.1, NP_001178824.1
RefSeq Size	5055 bp
RefSeq ORF	5055 bp
Locus ID	365298

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