Scrib (NM_001191879) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1565055
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217678 representing NM_001191879 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGTGCATCCCGCTCTGGCGTTGCAACCGGCACGTGGAGTCGGTGGATAAGCGGCATTGCTCGC TGCAGGTTGTACCAGAGGAGATTTACCGCTACAGTCGTAGCCTGGAGGAGCTGCTCCTCGACGCCAACCA GCTGCGCGAGCTACCTAAGCCCTTCTTCCGGCTATTGAACTTGCGGAAGTTGGGCCTCAGTGACAACGAG ATCCAGCGGCTGCCTCCTGAAGTGGCTAACTTCATGCAACTGGTGGAGCTGGATGTGTCCCGGAATGATA TCCCCGAGATACCTGAGAGCATAAAGTTCTGTAAGGCTCTGGAGATTGCAGACTTCAGCGGGAACCCCCT CTCCAGGCTTCCCGATGGCTTCACACAGCTGCGCAGCCTGGCTCACCTGGCTCTGAATGATGTGTCCCTG CAGGCACTGCCTGGAGATGTGGGCAACCTGGCCAATTTGGTGACCTTGGAACTGCGGGAGAACCTGCTTA AATCTCTCCCTGCGTCCCTGTCTTTCCTTGTGAAGCTAGAACAGCTGGATCTGGGAGGCAATGACCTGGA AGTGCTGCCTGACACTCTGGGGGCTCTGCCTAACCTACGGGAGCTGTGGCTAGACCGAAACCAACTGTCA GCGCTGCCCCCGGAGCTAGGCAACCTGCGGCGGCTGGTATGCCTGGACGTGTCAGAGAACAGGCTGGAGG AGCTACCCGTGGAGCTGGGTGGGCTGGCACTGCTCACAGATCTGCTGCTTTCCCAGAATTTGCTTCAGCG GCTGCCAGAGGGCATTGGTCAGCTGAAGCAGCTGTCTATCCTGAAGGTGGACCAGAACCGGCTATGTGAG GTCACTGAGGCCATAGGGGACTGTGAGAACCTCTCGGAACTGATCCTCACAGAGAACCTGTTAACGGCTT TACCCCGCTCTCTGGGCAAACTGACCAAACTGACGAACCTTAATGTGGACCGGAATCATCTTGAGGTGCT GCCACCTGAAATCGGAGGCTGCGTAGCCCTTAGTGTCCTCTCCTTGAGAGACAATCGCCTGGCTGTCCTT CCTCCTGAGCTTGCCCATACAGCTGAGCTGCATGTGCTTGACGTAGCTGGGAACCGGCTGCGGAGTCTGC CGTTTGCACTCACCCACCTCAACCTCAAGGCACTGTGGCTGGCTGAGAACCAGGCACAACCCATGCTCCG CTTCCAGACCGAGGATGATGCCCAAACTGGTGAAAAGGTGCTTACCTGCTACCTGCTGCCCCAGCAGCCC TTGCCAAGTCTCGAAGACCCTGGACAGCAGAGCAGTCCCTCAGAGAGCTGCAGTGATGCCCCTCTGAGCC GTGTCAGTGTCATCCAGTTTGAGGACCCCCTTGAAGGTGAAGAAGATGCTGAGGAAGCTGCAGCCGAGAA GCGGGGCCTACAGCGTAGGGCCACACCTCATCCCAGTGAGCTCAAGGTGATGAAGAGGGGCATTGAGGAG CGCCGAAACGAGGCCTTCGTCTGCAAGCCTGACCCCAGCCCACCCTCACCTTCAGAGGAGGAGAGGAGGC TGAGCGCAGAGTCTGCCCTGAGTGGTAACTCTGTCCCGTCGGCAAGCACAGCCTCCGATGGTGAACCTGA GACCCTCCCGGCTGAGGTACAAGGGCTAGGCCAGCAAGAGGCCATGCCTGGTCAGGAAGAGTATCCAGAG GAAGACTATAATGAGCCCACAGTGCACTTTGCAGAGGACACACTGATCCCTAGGGAAGATGGTGAAGGTG AAGAGGGACAGCCCGAGGCTCCCTGGCCGCTGCCCAGTGGCCGACAGAGGCTCATCCGCAAAGACACACC CCACTACAAGAAGCACTTCAAGATCTCTAAGCTCCCACAGCCCGAAGCTGTTGTGGCCCTGCTGCAAGGG GTGCAGACAGACAGGGAGGGCCCCACTGCGGGTTGGCACAATGGCCCACACACACCTTGGGCTCCTCGAG CCCATGAGGAGGAAGAGGAGGAGGAGAACAGGGATGAAGAAGAAGGCGAGGCCAGCATGGAAGAAGATGA CAAAGAAGAGGCTGTGGCTTCTGCTCCCTCTGTCAAGGGGGTGTCGTTTGACCAGGCCAATAACCTGCTG ATAGAGCCTGCTCGCATTGAGGAGGAAGAGTTAACACTCACGATCGTGCGGCAGACAGGGGGCCTGGGCA TCAGCATCGCAGGGGGAAAGGGCTCTACCCCCTACAAAGGAGACGATGAGGGCATTTTCATCTCTCGAGT GTCTGAGGAGGGTCCTGCAGCCCATGCTGGAGTCCGAGTCGGTGACAAACTTCTCGAGGTGAATGGTGTG GCCTTGCAAGATGCAGAACACCATGAGGCTGTGGAAGCACTTCGGGGGGCCGGTGCTGCTGTGCAAATGC GAGTGTGGAGGGAAAGAATGGTGGAGCCTGAGAATGCAGTCACCATCACCCCTCTCCGCCCTGAAGATGA CTATAGTCCCCGGGAGTGGCGGGGAGGGGGCCTGCGCCTGCCCCTGCTTCAGCCAGAGACTCCTGTACCC CTCCGCCAGCGCCACGCTGCCTGTCTTGTGCGCAGTGAAAAGGGGCTGGGCTTCAGCATTGCTGGTGGGA AGGGCTCCACACCTTACCGGGCTGGCGATGCGGGCATTTTTATATCTCGCATTGCAGAGGGAGGGGCTGC CCACCGGGCAGGCACTCTACAGGTTGGCGACCGTGTTCTCTCGATCAATGGAGTGGACATGACTGAGGCA CGGCACGATCATGCTGTCTCCCTGCTGACTGCTGCTTCCACTACCATCTCCCTGCTTCTGGAGAGGGAAA CTGGAGGGGCTTATCCACCCAGCCCCCCACCACATTCCTCTTCAACCCCTACTGCTACTGTTGCTGCTAC CATGACCACTGCCATCCCCGGAGAACCTCTGCTACCTAGACTGTCCCCTAGCCTCTTGGCCACTGCCTTG GAAGGACCATACCCTGTGGAGGAAATTTGCCTGCCCAGGGCTGGTGGCCCATTGGGGCTTAGCATTGTAG GAGGTTCTGATCACTCCAGCCATCCGTTTGGTGTCCAGGATCCTGGTGTATTCATCTCCAAGGTGCTTCC TCGGGGCCTTGCTGCTCGCTGTGGCCTTCGGGTTGGGGACCGCATCCTAGCAGTGAATGGGCAGGATGTT CGGGAGGCCACACATCAGGAGGCAGTTAGTGCCCTGCTCAGGCCCTGCCTGGAGCTGTGTCTGCTTGTAC GGAGGGACCCACCACCCCCAGGCATGCGGGAACTCTGCATCCAGAAGGCCCCTGGAGAGAAGCTGGGTAT CAGCATCCGAGGAGGCGCCAAGGGTCATGCGGGGAACCCGTGTGATCCTACAGATGAGGGCATCTTTATC TCCAAGGTGAGCCCTACAGGAGCTGCCGGGCGTGACGGGCGGCTGCGTGTGGGGCTGCGGCTGCTAGAAG TGAACCAACAGAGCCTCCTGGGCCTCACGCATGCTGAGGCGGTGCAGCTGCTGCGCAGCGTGGGTGACAC CCTCACCGTGCTTGTCTGTGATGGCTTTGACACCAGCACCACCACAGCCCTGGAGGTGTCCCCAGGTGTC ATAGCCAACCCATTTGCAGCAGGCCTTGGCCACAGGAACAGTCTGGAAAGCATTTCATCCATTGACCGGG AGCTGAGTCCTGAAGGCCCAGGCAAGGAGAAAGAGCTGGCCAGTCAAACCCTACCCTGGGAGCCTGAGTC TGCAGAGACCACGGGTCGGAATCAAGAGCCCCTCAAGCTGGATTACCGTGTCCTGGCTGCTGTGCCCAGT GCTGGGAGTTTGCAAAGAGGACCCTGTGCGACCACTGGAGGGAAGATGACTGAGGCCCCCTCTTCCCCTG GCAGTCAGCAAACAAAACCGGGGGTGATCCAGCCATTGGCTCAGGCCTGGCCAAGGGACTCCCCAGCCCC CAGGGGCAGAGATGGTCCCTGTTCTCCGCCCTCCCCTGACGAACTGCCAGCCAATGTGAAGCAGGCGTAC AGGGCCTTCGCAGCTGTGCCCACCGTACATCCTCCTGAGAACAGTGCTACCCAGCCTCCAACACCTGGTC CCGCAGCCTCCCCAGAGCAGCTGTCCTTCCGTGAACGACAAAAGTACTTTGAGCTGGAGGTACGGGTGCC TCAAGCAGAGGGTCCCCCAAAGCGTGTGTCCCTGGTGGGTGCTGACGACTTGCGGAAGATGCAGGAGGAG GAAGCTCGCAAGCTGCAGCAGAAGAGGGCACAGATGCTTCGGGAAGAGGCAGTAACCTCAGGACCTGACA CGGGGCTCGCCTCAGACAGGGAGTCCCTGGATGATGAGCAGGAGGCTGAGCAGCCCTGGGCCGGGCCAAG CCATGCAGGAGGCCTCAGCCCCTCTTCACCCACACCCCTGGGAGGCAGCGCCCCTGTGAGGACAGCCAAA GCTGAGCGACGTCATCAGGAGCGGCTGCGTATGCAGAGCCCTGAGCTGCCCGCCCCAGAGCGGGCCCTGT CTCCTGCAGAGCGAAGGGCTCTAGAGGCCGAGAAGCGAGCACTGTGGAGAGCAGCCAGAATGAAGTCTCT GGAGCAGGATGCCCTCCGTGCACAGATGGTCCTCAGCAAGTCCCAGGAAGGCCGTGGCAAGAGAGGACCC TTGGAGCGGCTGGCTGAGGCCCCTTCACCTGCACCCACACCATCACCTACACCATTAGAAGACTTTGGCC TCCAGACCAGTACCTCCCCTGGACGCTTGCCTTTGTCTGGAAAGAAGTTTGACTACAGGGCCTTCGCAGC CCTGCCTTCTTCCAGGCCTGTGTATGACATCCAGTCACCAGATTTCGTGGAGGAGCTGAGGACTTTGGAC CCATCTCCCAGTCCTGGCCCACAGGAAGAAGATGGAGAGGTGGCTCTGGTGCTCCTTGGCAGGCCCTCAC CTGGCACTGTGGGCCCTGAAGACATGACGCTATGCAGTAGTCGTCGGGCCATGCGGCCAGGACGCCGGGG CCTGGGCCCTGTGCCCTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191879
Insert Size	4992 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191879.3, NP_001178808.1
RefSeq Size	5547 bp
RefSeq ORF	4992 bp
Locus ID	362938

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