Tns3 (NM_001170459) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564174; tensin3; TSN3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217626 representing NM_001170459 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACAGCCACGAACTGGATCTTACCTATGTCACCGAGCGTATTATCGCAGTGTCCTTCCCTGCCA GTTGCTCGGAGGAGTCCTACTTGCACAGCCTGCAGGAAGTCACGCGCATGCTCAAGTCTAAGCACGGAGA CAACTACCTGGTATTAAACCTTTCAGAGAAGAGATACGACCTTACAAAGCTTAATCCAAAGATCATGGAT GTGGGCTGGCCCGAGCTGCACGCACCACCTCTTGACAAGATGTGCACCATATGTAAAGCCCAGGAGTCCT GGCTGAACAGCGACCCCCAGCACGTGGTCGTCATCCACTGCAGGGGCGGTAAAGGACGAATTGGAGTGGT CATATCGTCCTACATGCACTTCACCAATGTCTCAGCCAGTGCCGACCAGGCACTGGACAGGTTTGCAATG AAGAAGTTTTATGATGACAAAGTCTCAGCTTTAATGGAGCCCTCCCAGAAGCGGTACGTGCAGTTTCTCA GTGGGCTGCTGTCCGGAGCCGTGAAGATGAACGCCTCACCCCTGTTCCTGCACTTCGTCATCCTGCATGG CGCCCCCAGCTTTGACACTGGAGGAGCTTGCCGGCCCTTTCTGAAGCTCTACCAAGCCATGCAGCCTGTG TACACATCTGGGATCTACAATGTGGGCTCTGAAAACCCCAGCAGGATCCGCATTGCCATAGAGCCTGCCC AGCTGCTGAAGGGTGACATCATGGTGAAATGCTATCACAAGAAGTTCCGCTCGGCCACCCGCGATGTCAT TTTCCGCCTGCAGTTCCACACGGGAGCTGTGCAGGGCTACGGGCTCCTGTTTGGGAAGGAAGAGCTGGAC AGCGCCTGCAAAGATGACCGTTTTCCTGACTATGGCAAGATTGAGCTGGTCTTCTCGGCCACACCTGAGA AGATTCAAGGATCTGAGCATCTGTACAGTGACCAGGGAGTGACCGTGGACTATAACACAGCAGACCCCCT GATTCGATGGGACTCCTACGAGAACATGAGTGCGGATGGAGAAGTGCTACACACACAGGGCCCAGTGGAT GGCAGCCTGTATGCCAAGGTGAGGAAGAAGAGTGCCTCCGATTCTGGCATCCCCAGTAGCCCTCAGGGCA TGCCAGCCACCAGCAGTCCAGACCACGGTGACCACACCCTGTCAGTCAGCAGTGACTCGGGTCATTCTAC TGCCTCAGCCAGGACTGATAAGACAGAAGAGCGCCAGACCCCAGGAGCACGGAGGGGCCTGAGCCCTCAG GAGAAGGCAGAGTTGGACCAGCTGCTCAGTGGGTTTGGCCTGGAAGACTCTGCAAACTCCCACAAAGACA TGACTGACATGCGCAGCAAGTACAGCGGGACACGCCACGTGGTGCCAGCCCAGGTCCACGTGAATGGAGA TGCTGCCCTAAAGGACCGGGAGACCGACATTCTAGACGATGAGATGCCCCACCATGACCTGCACAGTGTG GACAGCCTGGGTACTCTCTCCTCCTCCGAAGGGCCCCAGTCAACCCACCTGGGTCCTTTCGCCTGCCTCA AGAGCAGCCAGAACTCCCTCCTGTCTGATGGCTTTGGCAATGGTGTTGCTGAAGATCACAATGGCGTCCT TTCTCCAGACCTGGGCCTTGGTGTAGACTCCGTTTATGAGCGGGAGCGGATGTGTGGGGGCCGAGAGCAA AAGCAGCTGCAGCCCCTGTCGAGGAAGCCCTCTGCACCCACCCCGACGCAGGCCTATGGGCAAAGTAACT ATTCCACCCAGACTTGGGTCCGTCAGCAGCAGATGGTGACTGCTCACCAGTACAGCTTTGCCTCGGATGG GGAGGCCAGGCACGGCAGCCGCAGTGCAGTGGACAGTGCTGGCCTTGCCCAGCCCTCACCCCACATCCCA GTTACTCCCACCCATGGAACCAGCAGCAGAGTGGCTGTTCAGAGAGGCATCAGCAATGGGCCAAATCCCC CTGACGCACAGCAGCTCTGTCCTAGTAAAGCACTCCAGCCCCGGTTTCCAGATGACAGAGTCATGAATGG AGTAGTACATCAGGAGCTTAACCCTGGCCCCTCCCCAGGCTCTCCTACTCTGGATATCGACCAGTCCATT GAGCAACTTAACAGGCTGATCTTGGAGCTGGACCCTACCTTTGAGCCCATCCCCACACACCTCAGTGCCC TTGGCCTCTCAGCCAATGGCTCTATGTATCCTGATGGTGTGGGAAGTGGGCTCCGGTGTGGTGGCAGGCT GGACCCTGTGGATGGCCCTGGCCGGAGTCCTGGCCGGCAAGGTGATGACCCCGTCGGGGGACGGCTTCGG AAACTGAGCATCGGGCAGTATGACAATGATGCCGGGAGCCAGGTGACCTTCTCTAAGTGTGGATGGGGAA AGGCTGGGGCGGACTCAGCCCCAAGCTTGGGATCCTTTTCCTCTCCTGCTGACATCAAAGAGACGGTGAT CACGGCCTATCCCCCTGACCTCGATGTGATCGATGGTAGGATTCCAAACAGCAAGGAGTCACCTATATGT CTGACCCCAGCATTCCCTGTGTCTCCAGAAACGCCATATGTGAAAACTTCGCCACGATACCCTCCGTTCA GCCCTCCTGAGCCCCAGCTGAGCATCCCAGCCAGTTTACACAAAGGGCGAGAACCACGAGGCTGCACAGA GATTATCAGCCACACTGTGGGGATGTCAGAGAGCCCCCTCGGATCCAAACCCACCCTGCTTCGGGCTGAT ATGCCTGCAACACCCAACTTCCAGCAGGTGTTCGCGGCATCCTGCACAGTTTCCAGCAACGGCCCCGGCC AGAGGAGAGAGAGCCCGCCTTCTGCAGAACGCCAGTGGGCAGAGAGCAGCCCGAAGTCCACCCTCACCCT ACTGGGGAACAACCGCCCTTCCGAAAGTCCCCTCGGCACCCATGAGTTCTGTAGCTCTGGTAAGAACTCA CCAGGGCTGCCCTGCTTCCAGTCCTCAGAGCTCCAGGCCTCTTTCCACAGCCATGAGCTGTCCATGTCAG AGTCTCAGGATGCTATGCCTCCTGCAGGCAGCCAAACCTTCTTGGGTTTCAACACAGTAACTACAGTAAC AAGTGGCCTTCCACCCAGTGAGGATGCTGGCACCTTGCTGGTTAATTCCCACGGTCCATCACCAGTCCCT GGAACCCCTCTGACAACGACAGGGGCTGCGGACAACGGCTTCCTGTCCCACAACTTTCTCACGGTATCAC CTGGAGCCAGCAGCCACCACAGTCCGGGCCTGCAGAACCAGGGCATAAGCCTACCCGGGCAGCCACCCCT CCCGGAGAAGAAGAGAACCTCAGAGGGAGATCGCTCTTTGGGCTCAGTCTCCCCTTCATCCAGTGGCTTC TCCAGCCCCCACAGTGGGAGCACCATGAGCATCCCCTTCCCGAATGTCCTTCCAGACTTCTGCAAGCCTT CAGAAGTGGCCACACCTTTGCCAGATAGCCCAAATGATAAACTCGTGATTGTGAAGTTTGTTCAAGACAC TTCGAAGTTCTGGTACAAGGCAGACATCTCAAGAGAACAAGCCATCGCCATGTTGAAGGACAAAGCTCCT GGGTCATTCATCGTGAGGGACAGTCACTCCTTCCGAGGGGCCTACGGCCTGGCCATGAAGGTGGCCACAC CTCCTCCATCGGTCTTGCACCTGAACAAGAAAGCAGGAGATTTGTCCAATGAACTCGTCCGGCATTTCCT GATTGAGTGTACCCCAAAGGGAGTGCGGTTGAAAGGGTGCTCGAACGAACCATACTTCGGGAGCCTGACG GCTTTGGTGTGCCAGCATTCCATCACTCCCTTGGCTTTGCCCTGCAAGCTGCTCATTCCAGAGAGAGATC CGCTAGAGGAAATAGCAGAAAACTCTCCCCAGACAGCAGCCAACTCAGCAGCCGAGCTGCTGAAACAAGG AGCAGCCTGTAATGTTTGGTACTTAAACTCTGTGGAAATGGAGTCCCTCACTGGTCACCAGGCAGTCCAG AAGGCCCTGAACATGACCTTGGTTCAAGAACCTCCTCCAGTGTCTACGGTGGTACACTTCAAGGTGTCAG CCCAGGGCATCACCCTGACTGACAATCAGAGAAAGCTCTTCTTCCGGAGACATTACCCTGTGAGCAGTGT GATTTTCTGTGCTTTGGACCCACAAGACAGGAAGTGGATCAAAGATGGCCCTTCTTCTAAAGTCTTTGGA TTTGTGGCCCGGAAACAGGGCAGTGCTACAGATAATGTGTGCCACCTGTTTGCAGAACATGACCCCGAGC AGCCTGCCAGTGCCATTGTGAACTTTGTGTCAAAGGTCATGATCGGCTCCCCTAAGAAGATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170459
Insert Size	4335 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170459.1, NP_001163930.1
RefSeq Size	7165 bp
RefSeq ORF	4335 bp
Locus ID	360980

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