Scaf11 (NM_001271170) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sfrs2ip; Srsf2ip
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217625 representing NM_001271170 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGAAACCCGTATATAATCAGAGTGTGGGTGATCAGGAGAGTGAAGATGCGGAAGGTGAAGAAA ACAGAAATAATGCTGATACTTCTGGCTTACTGTACAGTGAGGTGGACAGATGTCCAATCTGTCTTAGTTG CCTATTAGGGAAGGAAGTTGGTTTTCCAGAAAGTTGTAATCATGTCTTCTGTATGGCCTGTATTCTTAAA TGGGCAGAGATACTGGCTTCTTGTCCAATCGATCGGAAACCCTTTCAGGCAGTGTTTGAACTCCGTGCAT TTGAAGGTTGTGCTAAGATTCAGGTAAAAAGACGGTCGAGAGAATTAGACGACAAGGAAAATGAGGAGTC TTTTAAGAAACAACTCGTTTATCATGAAAGTTCCAAAAGCGACAAGAGAAAAGGAAACACTGTAAGAGAA GATCTGTTATGTGAGAGATCTGATGACTTGAAGGTGTTGTATAGAAACTTTTCAAACAATAAAATGGGTG GAAAGAAAAATGCTACAGTAAAGACAAACAAGGTTCAAAGATTGAATCAGTGTACAGATTCTTGTGTGAG AAGTGACATGCCCAGTGTATCTTCATGTGACGGCCACAGTTGGGGACCTCGCACCTACAGAGTTTCCTGT ACAGAATCTATAGAAGTCAATGAAATCAATGCATTGATTAGGCAGAAACGACAGGAACTGGAGTTGTCAT GGTTTCCTAATACATTACCTGGGAATGGAAGAGTTGGCTCTGTACCCTGGAGTGTGGAGACAGCAGTTCT TCCTCTGGTTTCCTCAGTGCTGCCAAGGACGATTTTTCCAGCAAGTACCATGTCACTAGAAAATTTTGGT ACTTCTCATAAGGGGTATGCACTAGCACATACCCAAGGAGGAGGAGAAAAAAAGCAAACTTCAGGTACAT CAAACACCAGAGGATCAAGACGAAAACCTGCAACAACTGCTCCTACAAGGAGGTCCACACGAAACACACG CGTTGAGACAGTCAGTCCATCCCAGAAGTCCCCAGTGCCAAACTACTCTGAGTGTGATGCCCCAGGGAAT AGTAATTCCTCTGTCAGTATTTCCCCTCCAGCTGAATCAGAAAAGCAAACAAGACAGGCTCCAAAACGGA AGTCAGTAAGGAGAGGAAGAAAACCACCTTCTTTGAAAAAGAAACCTCGGAAATCTCTACCTCCTGCTGA AAAAACCACATCTAGCGATTCAGTAGAGGAAGACACTGTGGATTCTGACACTCCACCCGTGTTGGAAAAG GAGCAGCAATCATGTGTAGAGAGTAGTAGCATTTGCTCTATTCAGACAGATGAAGAGAATCATTTGGCTA AGTGCTTGGAAAGTTGCGATGAACAAACAGAAGAAAATGAACAAACCAAGAATCATGAGATCGAGGAACA AACAGAAATTTTAAATTCTGAATCTTGCACTCAGTATCCTCCTGTGCTTGTTGGAGAGGATTCAGGGATA GAGGCTAAGGAGTTATGTGTAGACAATGATGTTTCTACAGATTCTTCTCTAAAAGGAAGTGATCCATTGG AAAATCAAGAACAGGGCTCTGGAAGCTCAGGGTCAGAGGTGCAGGCATCCGTGTGTACGGAGAGTCCGCC TGAGGATTTCCTTACGTGTCCACCGTCTGACATTGAAGGACACCAGCCAGTGTCCGGTCCCCTGGGTGAA GTATCTGAAAACGCCACCCCAGTGCTTAGTGATGAAAGAGCAGAGGACAGTCTCATGGTAGGAAATGCTG ATTTGAATGATTCTGTAGAGAACACAGAAGCATTTGTAATGAGCCCCAAAGTGGAGTCTTCTGAGGGTGA AATTACACATGGACTGAACAGACATTGTATTCCCAGCTCAGACACGGAGTTGCCTGAGCTTATTCAAACA GAAAATACAGAAATAATTCCAACATGTGGCACTTTAGAAAATGAAAGTTCTGGTGCTGTTCAAGGTTGTG AAGATACCTTACTAAAACATAATCTTGACAACACCCAATTGGATAACTCCTTAGAAGAAAAGACAGACTC TCTGGTTGAGCATCCCATCCATGCAGAGTTACCTCACAAAGAAGTTGAACAGAGTGAGAAACATTTCAGT GAAGATAATAATGAAACAGTACCTATGGAGTGCGATTCTTTCTGTAGTGACCAGAATGAGTCTGAAGTAG AGTCATCAGCAAATACTGACCCCAAACAGTTGAGTGAAAATTCTGTGACACACAGTTCTGACAACAAGCT GTCTTCTGATCCTGTAGTTGAGAATGTTGAAACTGTAGCTCAACCAGCTGAAAGCCTAGTGGATAAAGCC CCAAAGCCTCGAACTCGGAGATCTAGATTCCATTCTCCGTCTACAACTTGGTCTCCCAACAAAGATGCTG CACAAGAAAAGAGGCGCGCTCAGTCTCCATCTCCGAAAAGAGAGACTGCAGTAGAAAGTAAGAGTTCTCA GTCACCATCCCCCAAGAGAGAGTCTGCCAGAGGACGAAGAAAATCCCGTTCTCTCTCACCAAAAAAAGAT GTATCAAGAGAAAGGAGGCAGTCTCGGTCTCCAAAGAGAGAGAATGCTAGGGAAGCTAAAAGGTCTGAGT CAGGCTCCCCAAGAAGAGACACTTCTAGAGAGAACCAGAGGTCTCAGTCCAGGGTGAAAGATTCTTCCTC CAGAGAAAAATCTAGGTCCCGGAGCAGAGAAAGAGAGAGTGACAGAGATGCTCAGAGGAGAGATCGAGAT AGAGAGAGGCGAGCCAGAAGGTGGTCCCGGTCTAGATCTCGCTCTCGATCGCCATCAAGATCAAGAACGA AGAGTAAGAGTTCATCATTTGGTAGAAATGAAAGAGATACTTACTCTCCCCGGTGGAGGGAAAAATGGAC AAATGATGGTTGGAGGTGTCCCAGAGGGAACGACCGGTACCGGAGGAATGATTCAGAGAAACAGAATGAA AACACAAGAAATGAAAAAGATGACATCACTGCAGATAATACTAATGATCCCAGTTCCACAGATAAGCATA GAGATGACTGTCCCAGCTGGGTGACAGAAAAAATAAACTCTGGGCCAGATCCAAGGACCAGAAATCCAGA AAAGTTAAAAGATTCCCACTGGGAAGAAAATAGAAATGACAATTCAGGGAATTCTTGGACTAAAAACTTT GGCCCAGGCTGGATGTCCAGCCGTGGTCGAGGAGGCCGTGGGAAGGGTGCTTACAGAGGTAACTTTGCCT GTAACGACCAGAGCGAGAACAGGTGGCAAAGCCGCACCCCCCTCTCAGGGAGCGACTCCTTCAAGTCTGT GGAGCAGCAGCCCAGTAAGCGGAAAAGCGAGCAAGAACTGTCCTTCGGCACTCCAGCAGACAGGTCGGGG TGGACCTCCGCCTCCAGCTGGGCGGTGAGGAAGACTCTGCCAGCCGACGTGCAGAACTATTACTCTCGGC GCGGCAGGAGCTCTTCAGGCCCACAGTCTGCGTGGATGAGACAGGAAGAGGAGACGCCGGAGCAGGATTC TAACTTAAAAGACCAAACAAACCAAATTGATGGTTCTCAGTTACCGGTAACTATGCTGCAGCCACACATG AGTGTAATGCAGCCTCCGGTGAATGCCCAGCACCAGCCTGTGGGCATCTTCCCGTACCCAGTGGGTGTTC ACGCCCCTATGATGAATATGCAGCGGAATCCATTCACCATGCACCCTCCGATGCCCCTGCATCTCCACAC GGGAGTACCTCTCATGCAGGTAGCTGCCCCTGCAAGCATACCTCAGGGACCGCCACCACCGCCCCCTCCC CCGCCATCCCAGCAGGTCAACTACATCGCCTCACAACCAGATGGGAAACAAGTGCAGGGCTTTCCGAGTG CATCTCATGTAAGTAATAACATGAATACACAAGTGTTGCCTGCTCCATCAGCAGCCCCAGCAAACACCGG ATCAGTTCAGGGACCAAGTTCTGGTAATACTTCGTCATCAAGTCACGTCCAAGCCGCTAACGCTGCTGTA AAATTGGCAGAAAGCAAAGTAAGTGTTACGGTGGAGGCCAGCGCAGATAGCTCGAGGACAGACAAGAAAT TGCAGATTCAAGAGAAAGCAGCACAGGAGGTGAAACTAGCTATTAAGCCATTTTACCAGAATAAAGACAT CACTAAGGAAGAGTACAAAGAGATCGTTCGGAAGGCTGTGGACAAAGTTTGTCATAGTAAGAGTGGAGAA GTAAATTCTACTAAAGTGGCAAATCTGGTTAAAGCGTATGTAGACAAGTACAAATACTCTCGGAAGGGAA GCCAGAAGAAAACGCTGGAAGAACCCGTGTCTACTGAAAAAAACATAGGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271170
Insert Size	4323 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271170.1, NP_001258099.1
RefSeq Size	7478 bp
RefSeq ORF	4323 bp
Locus ID	312030

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