Nacad (NM_001100655) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217615 representing NM_001100655 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGGAGGCTGCGGGCGCTGAGCTGCCGCTGCCTGAGGCAGGAGGGTCTGGATCCCGCACAGATC TCCCATGTGATGCAGCTATTGCAACCATCCTGAAAGGGGACCAACGAGAGCCCCATGGCCTGACCCCAGG GCCTAGCCCTCTGGCGCTCACATTCCTGTCCAGCAAGCCAGGTGCCCGGCCCCAGCCTGAGGGGGCCAGC TGGGATGCAGGGCCCAGTGGGGCTGCCTCAACCTGGGTGGACCCGGCAGAGGGCAGCCCGAGCCTAGTGG TCCTCCCTGAGGGCCTGCCTCTCCGGCCTGTGCCTGCCGATGTCCCTCTCCCCACCACCCTGGAGCCCCG CATCGTGATGGGTGAGGAAACATGCCAGGTCCTCCCTTCACCCAGGGCGGCCTGGCCAGTGCTCAGGGAC CGGGAGGGTGGGCACACTGCCCTGCACCCACCTCCGGAGCTGTGTTCTCAGGGTGATCCTCCTGTGCCTT CCCCTCCCCCTGATCTCGATTCTTACTTCACACCCCCCTCTACTCCCACCAAGACCACCTATGCCCTGCT TCCTGATCATGGGCCCCACAGGGGTGCCTGGGGTCTGGAGGCCGAGCTGCTGGATGAGTTACTGGACTCA ACACCAGCCTCACCCTCCGGCTCCTACATTACGGCAGACGGGGACAGCTGGGCTTCATCGCCTTCCTGTT CCCTCAGCCTGCTGGCCCCTGCCGAGGGGCTGGACTTCCCCTCCGAGTGGGGCCTCTCCCCATCAGGGTC AGTGGTAGATGAACTAGAGCCCCACCCCACGGCACCTCCCGAGCCACCATCCTCTGAGTCCAGCCTCTCA GCAGACTGCAGCTCTTCCTGGAGCCAAGAGGGCCATTTCTTTGACCCAAACTGCCTGGCCAACGACCCGA TGATCCCTGCCGCCCTCCTGCCCTTCCGGGGTAGCCTCATCTTCCAGGTGGAAGCTGTGGAGGTGACACC GTTGCCCCAAGAGGAGGAGGAGGATGAAGAGGAGGGAGCTGCTGCTGCTGCTGCTGCTGCTGCAACCCCG GATGGAGACCTGGCTGGAGAGGGTGAGGACGACAGCACATCTGCCTCCTTCCTGCAGTCGCTGTCCGACC TGTCCATTATCGAGGGCATGGATGAGGCCTTCGCCTTCCGGGACGATACCTCGGCGGCCTCCTCTGACTC AGACTCAGCTTCCTACGCAGGGGCAGATGATGACAGGCTATACAGTGGGGAGCCCCATGCCCAGCCCAGT GCTCAGAATATAGAGCAGGAGTACAGGAGCAGGACCGCATTTCCAGGGGCAGCAGAGTTCACACCTCAGA CATCAGAGCAAGAGATCTGCCTAACCGATAGCCAGGAGTCGGCTGCAGAAATAGCAGAAGAGATTCTAAC TTTAGGCATAGAGTCCGAAGCTACTAGGACACCTCCTGATCGGCAAACAGCTCCAGACCCCCAGGTGGAG GAAGCTACCACAGTGACCCCATGGGTGGAGAACAAAGATGACTCAGTTGCTGAACAAGCATCCAAGGCTC TTCCTGAGCCCTGCCAGAAGGGGACAAGCATGATCTTAGGCTGTAAACCCCTCACTGCAAAGGCAATTCC AGATCTGCAGGAAGGAGCAGGCCCTGCCATGTGTCCAGTCCTTCCTGAGAACTTGAAGGAAGGAGGCCAG GGCCTTCCCTCCACAATGGAGTATGTGGCTGTGGCTTCAGTGGGGCCCTGGAAGGCTGAGGGAGGTGTCG CAATACCGCAGGACCCCTTTATGACATTACCCCCACTCTTGCAAAATACAGATCCCACCTCAGGCCCCGA GTCTGTGGCTGTAGTCATGCTAGAGCCCCAGCAAGATGAGGGATGTGTCACAGTACTCCAGGATGTTCCT GTGGTATCACTTCCATCCCCACAAAGTACAGATCCAACCCCAGGTCCTGAGGCTGGGGCCATGGCTACAT TTGAGTTCCAGCAGGCTAAGGAAGGTGTCCCAGAGCTTCAGGATACCCCTGTGGCATCATCCCCAGCCCT GCAAGCCTCAGACCCCACCTCAGAGCCAGAACCTGAGGCTGTGCTTACACTGAGGTCCCAGCAGGATGAA GAGATTGTCACAGTACCCCAGGAGTCTCCTACAGCATCATCTGTACCCCTGCAAAGCTCAGATCCCACCT CAGAGCCAGAACCTGAGGCTGCTGCCACACCGGAACCACAGCAGCCTGAGGAGGGTGTCACCATACCCCA GGTTGCCCCAGTAGCATTACCTTCACCACTGCAAGGCTTAGATTCAACCTCAGACCTAGAATCTGTGGTT GCAGGCACAATGGAGTCCTGGCAGGATGAAGGGATTGCCACAGCAACCCAGGACATGCCTGTGGTGGCAC CTCCACCCCTTGGAGGTATAGAGTCCACCTCAGACCCAGAGCTGAGTGCTCAAGACACCCCTCAGGCCCT GCAGAGAGAAGCAGGCTACACCCCGGGAACCAAGCCTTCTGTCTCTGAGGCCCATCAGGAATTAGGTGTG GCCTTAGGACCGAGGCCAGTGCCTAAGGAACAGGATGCAAAGCCTCCACACTCTGAGCTCCCAGCTTCAG ACCAAGCCCAACAGAATGGCTCAGAGCCAGTTGTAAAGAATGACAACCTTGGGGTTCCAGGAGAAGGGTC AGGTCCCACTTTGAGCACAAAGACCTCTGAGCCCCTATCCTGTATGGGGGAGAAAGTAACTGAGAACCTA GCTGCACCCAAGCCAGGAGCATGTCTTGAAGCCCACGATGGGGCCAAGACACACTCACCTCAAAGACAGG CCTCGAGGGCCAAGAACAAGTGTGGGAGAGGCCCCAAACCACCAGGGCAGGGAAAGGGGTCCAAGTCAAC AGCATCGCAGGGTACCACGGAGACCTTCAAGGCGCATTCTGCTGCACGCTCTGAGACCAGCCAGCCACAG CTGATGAACTCCGCTGGGGAGGGAACAAGCCTGAGTGTCAAGGACTCCTCAGGCCCCAGGCTTCCTGTGG CTGTATCTGTACAATCCAAGCTTGGTTCCTGCCCAGGGTCCCCAGCAAGAGCCACATGCACACTGAGCAG GGTCTACTCTGAAGAGACTGCCAGGTGTGCACCACCCTTTCAGCACCCGGAGCCTGTGCTAGGCCTAGGC AGCGGAGAACAGCCCAAGGTGACCCTTGGTACCCTTAATCTCTCCTCAGAAAACTCTGCAGGTGACCTGC TCACCACACCTCAGAATAGGCTTCTGGACCCTGACCCTGACCCTTCTGCTCCCAGTGCCCTCAACAGGGC ATTCCAATCATCCCCAGGGCCCCCAGACCCTTGCCTGTGCCCCACTTCCCAGAAAGACCCTGTGGAAGAG GAGAAGCCCTCAGCCTCTCATGGTCTGAGGTCTCGGGCAGGAGTCCAGGGAGCTGCTGCCATTACCACCT CAGGATCCACAAAGCCTCTGGGAGCCCGGCAACGTGTCAGCCTCTCACCTCACTCCGCCAATCCCAAGGT GACCCCCACAGATGCCAAAGACCCGGCTTGCATCATCTCAAGCCCCTGCCAAGTGCCTCCTCCCTCTGGG ACCCAAAACCCATCGGGCCCTCGAGGGTTCCCAGCTCATGAACAAGAGGATGAGGACAGCCTGGAGGAAG ACTCGCAGAGGGCCCCAGGCTCCGGCCAGCATTCGGAGAGCCACGGGGAGTCGTCGGGTGAGCTGGATGA GCAGGATATCTCACCTCAGAAATCGCAGTGCCCGGCACAGGACCCAGCCGGCAGCAATGAGGAGACCACT GCCAAAGCCAAGCAAAGCCGCAGCGAGAAGAAGGCTCGAAAGGCAATGTCGAAACTGGGCTTACGGCAGA TTCAGGGAGTCACCAGGATCACCATCCAGAAGTCAAAGAACATCTTGTTTGTGATTGCCAAGCCAGATGT CTTCAAGAGCCCTGCCTCAGACACCTATGTGGTCTTTGGTGAGGCCAAGATTGAGGATCTGTCCCAGCAA GTACACAAAGCAGCAGCGGAGAAGTTCAAGGTGCCCTCAGAGTCCTCAGCCCTGGTCCCTGAGCTGGCCC CTGGGCCCCGGGTGAGGCCAGAGTGTGAGGAGCAAGAGGAGGAAGAGGAGGAGGTAGAGGAGGCCGGGCT AGAACCTCGTGACATTGAACTGGTGATGGCCCAGGCCAATGTGTCCAGGGCAAAGGCTGTGCGGGCCCTG AAGGACAACCACAGTGACATTGTCAATGCCATCATGGAACTGACAATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100655
Insert Size	4251 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100655.2, NP_001094125.2
RefSeq Size	4362 bp
RefSeq ORF	4251 bp
Locus ID	289786

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