Pard3b (NM_001191808) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217542 representing NM_001191808 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGACAGTGTGCTTCGGGAGGACCGGCATCGTGGTGCCCTGCAAGGACGGCCAGCTGCGCGTCA GCGAGCTCACCCAGCAGGCGCTGCAGCGGTACCTGAAGACCCGGGACCAGGATCCTGGATACTGGGTGAA GATTCATCACTTGGAATACACAGATGGAGGAATCCTGGATCCAGATGATGTCTTAGCAGATGTCGTTGAA GATAAAGATAAGCTGATTGCTGTGTTTGACGAACAAGAGCCACTCCAAAAGATTGAGAGCCCCAGTGGAA ACCCTGTGGATCGGCAGAGCCCAGATGCTTTCGACACAGAAGTGGCTGCCCAACTGGCTGCGTTTAAACC AATCGGCGGGGAAATTGTAGTAACCCCTTCTGCTCTGAAGTTAGGCACTCCGCTGCTGGTTAGGAGAAGC AGTGACCCAGCACCAGGGCCCCATGCTGATGCCCAGCCAAGCACTGCAAGCCCCAGTGGCCAGAGCTTGA AACCTGTTGTTCTGGATTCTGCCCAGAACTTGGAAAACAGAGACGTTATGAATGGTGAACAGACAGGGCT ACTTATGCTGCACAAAACGAAGAATGAACTGAGTGATATGACGAGAGCTGTGGAGATTTCTGGGGAAGGA GATCCCTTGGGGATACATGTGGTGCCCTTCTTTTCATCCCTGAGTGGCAGGATCCTAGGACTCTTCATCC GAGGCATCGAAGAAAACAGCAGGTGCAAGCAGGAGGGACTATTTCAGGAAAACGAATGCATTGTAAAAAT CAACAACGTGGAACTCCTGGACAAAACTTTTGCTCAGGCCCAAGATGTCTTCCGTCAGGCAATGAAATCT CCCAGTGTGGTCCTCCATGTGCTTCTGCCTCAAAATCGTGAACAATATGAAAAATCAGTCATTGGACAAC TGAACATTTTTGGCAACAATGACAGTGCTTCAAGAACAAAGGCACCACCTCCCACCCGTGGGAAAGCAGG GCTAAAGGCAGTAAATCCAACAAGAGCAAACAGCCCTGAAGGAGAGGAGTCACCTTCCCCGCAGCAAAGC AAGAGTCCCCGAGTTCCAAGATTAGGAAGAAAACCTTCCTCTCCCTCACTCTCCCCTCTCATGGGGTTTG GCAGCAAGAAAAATGCAAAAAAAATTAAGATTGACCTTAAGAAAGGCCCCGAAGGACTTGGTTTCACTGT GGTAACTAGAGATTCTTCTATACATGGTCCTGGTCCCATTTTTGTAAAAAATATCCTACCAAAGGGAGCA GCAGTAAAGGATGGCCGCCTACAATCAGGAGACAGAATTTTAGAGGTAAATGGCAGAGATGTCACAGGAA GAACCCAGGAAGAACTTGTGGCCATGCTGAGGAGCACTAAGCAGGGAGAGACGGTATCACTGGTCATTGC CCGCCAAGAAGGGAGCTTCCTGCCCCGAGAGCTGAAAGGAGAACCTGATTGCTATGCACTCTCCCTGGAA TCAAGCGAGCAGCTCACCTTGGAGATCCCCCTGAATGATTCCGGCTCTGCTGGTCTTGGAGTTAGCCTGA AAGGGAATAAATCTCGAGAAACTGGGACTGACTTGGGGATTTTTATCAAATCCATCATCCATGGAGGTGC TGCTTTCAAGGATGGCCGTCTCCGAATGAATGACCAACTGATTGCTGTTAATGGGGAAACTCTTCTGGGA AAGTCCAACCATGAAGCCATGGAAACACTGAGGAGATCCATGTCCATGGAAGGAAACATCCGGGGCATGA TCCAGTTAGTGATTCTGAGGAGACTGGAGAGACCACTGGAGGAGATTTCAGAATGTGGAGCACTTTCCAG ACCATGCTTTGAGAACTGTCAAGAAGCTCTGAGCGCCTCCAGGCGAAATGATAGTAGTTTATTATATCCG TGTGGCACCTATAGTCCACAAGATAAAAGGAAAGACCTATTGCTTCCCAGTGATGGGTGGGCTGAGAATG AAGTACCGCCGTCCCCGCCACCACATCCCAGTCTGGAATGGGGCCTTGAAGACTTCAGTCACAGCTCTAG AGTGGATTCCACAGGATATTTTCCAGATCAACATGTCAATTTCAGAATTGTGACACCAGTCGGGCAGCCC GAATCAATTAACCTGAAAGCCTCCAAGAGCATGGACCTTGTGCCAGACGAAAGCAAAGTCCAGTCACTGG CTGATCGCAGGTCAGACTCTCCAGGCAAAGATTTTGGTCCAACCCTGGGCCTGAAAAAGTCCAGTTCCTT GGAGAGCCTACAGACTGCTGTGGCTGAAGTCAGGAAGAATGATCTGCCCTTCCACAGACCCAGACCACAC ATGGTGCGAGGCCGGGGCTGCAATGAGAGCTTCCGAGCAGCCATTGACAAATCTTACGACGGGCCAGAAG AGGCAGAAGCTGATGGTCTGTCTGATAAGAGCTCTCACTCGGGCCACACAGCTCTGAATTGTGAGTCTGC CCCTCAGGGAAACCCTGAGCTAGATGATGTGGAAAATAAAGCCAAGAACGTCAAAAAAACAAAAGAGAAG GAGAAGAAAAAAGGAAAGGGCAAGATGAAAGTCAAGGAAAAAAAGCAGAAAGAGGAACATGAGGACACAG AGAGGAAAATGAAGAGGAAGGGCTTCGGCGCCATGCTGAGATTCGGAAAGAAGAAAGATGATAAAGTTGG GAAAACGGAACAGAAAGGTACACAGAAGTCCGGGCACCCAGGAGAAGAAGAGCTGGAAAGGATGAAGGAA GAAAGGGAGAGGATTGGAGCAAAACATCAGGAGCTAAGAGAAAAGCAGGCGAGAGGCCTTGTTGATTATG CCACTGTAGCGACTGGACCAGTGCACGATATAGACGACGATGAAATGGACCCCAATTATGCCAGAGTGAA CCACTTTCGGGAACCATGCACATCAGCAAATGTCTTTAGATCTCCATCTCCTCCTCGAGCTGGAACACTT GGTTACCCTAGGGATGGACGCCCACTGTCTCCAGACCACTTAGAGGGTCTCTATGCCAAGGTCAACAAGC CATATCATCCACCAGCCCTAGCTGACAGTGGACGACCCATGGCAGGAACCACTGACCGCATCCAGAAGCT TCGGAAGGAGTACTACCAGGCTCGGAGAGAAGGGTTCCTTTTGTATGAAGACGAAGAGGGAGTAACAAGG CCGTCTGACTATGACCTGCACTGGGTGTCTGGAAAGGGTCCAGACGGGAGCGCACACAACCTCCGCTTTG AGGGGATGGAGCGGCAGTATGCATCCTTACCCAGGGGAGGCTCAGCAGATCCTGTGGACTACCTGACAGC TTCACCCCGAGGGCGCTACAATGAAAGAGAGCTTCCATACTACCCAGGGCCTCATCCTGTGCATTCCCCA AGAGGGAGCTATCCCAGACCCCCGGATCTGAGGGTGACCGATCTACGATACCCACAGTACTACCCACCCC CACCAGCCCATCAGCATAAAGGACCCTTCAGACAAGATGTTCCTCCGTCACCACCTCAGCACCAGAGAGT ACCAGTCTACCAGGAGATGGGCAGGGCAGGGCCCCGAGGGGGCAGCCCTGACCAGTACCCCTATCGTAAC CAGGATCCCAGGCAGAAGAATCCCATGACTGCAGCAGTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191808
Insert Size	3612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191808.1, NP_001178737.1
RefSeq Size	3612 bp
RefSeq ORF	3612 bp
Locus ID	301455

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