Abl1 (NM_001100850) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Abl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217502 representing NM_001100850 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCAGCCTGGAAAAGTACTTGGGGACCAAAGAAGGCCTAGTTTGCCCGCCCTACATTTTATCA AAGGGGCAGGGAAGAGGGACTCATCGAGACATGGGGGCCCACACTGCAATGTCTTTGTGGAACATGAAGC CCTGCAGAGGCCAGTGGCCTCTGACTTTGAGCCCCAGGGTCTCAGCGAAGCAGCTCGATGGAACTCCAAG GAAAACCTTCTTGCTGGGCCCAGCGAAAATGACCCCAACCTTTTTGTGGCACTCTATGATTTTGTGGCCA GTGGAGACAACACTCTCAGCATCACTAAAGGCGAGAAGCTCCGGGTCTTAGGTTATAATCACAATGGGGA GTGGTGTGAAGCCCAAACGAAAAATGGCCAAGGATGGGTCCCAAGCAACTACATCACCCCTGTCAACAGC CTGGAGAAGCACTCCTGGTATCACGGGCCCGTGTCTCGAAACGCTGCAGAGTACCTGCTGAGCAGTGGGA TCAACGGCAGCTTCTTAGTTCGGGAGAGCGAGAGCAGCCCGGGCCAGAGGTCCATCTCGCTGCGGTATGA AGGGAGGGTGTACCACTACAGGATCAACACTGCCTCTGATGGCAAGCTCTACGTCTCCTCGGAGAGCCGC TTCAACACTCTGGCCGAGTTAGTTCACCATCATTCCACTGTGGCTGACGGGCTCATCACCACACTGCACT ACCCAGCTCCCAAGCGCAACAAGCCCACCATCTACGGCGTGTCCCCCAACTATGACAAGTGGGAAATGGA GCGCACAGACATCACCATGAAGCACAAGCTGGGCGGAGGCCAGTACGGGGAGGTGTACGAGGGCGTCTGG AAGAAGTACAGCCTCACTGTGGCCGTGAAGACCCTGAAGGAGGACACCATGGAGGTGGAGGAGTTCCTGA AGGAAGCCGCAGTGATGAAGGAGATCAAGCACCCTAACCTGGTGCAGCTGCTAGGGGTGTGTACCCGGGA ACCTCCGTTCTACATCATCACCGAGTTCATGACCTATGGGAACCTGCTGGACTACCTGAGGGAATGTAAC CGGCAGGAGGTGAGCGCTGTGGTGCTGCTCTACATGGCCACACAGATCTCATCAGCCATGGAGTACCTGG AGAAGAAGAACTTCATCCACAGAGACCTTGCTGCCCGGAACTGCCTGGTAGGGGAAAACCACTTGGTGAA GGTGGCTGATTTTGGCCTAAGCAGGTTGATGACAGGGGACACCTACACAGCCCATGCTGGAGCCAAGTTC CCCATCAAGTGGACTGCACCCGAGAGCCTGGCCTATAACAAGTTCTCCATCAAGTCGGACGTCTGGGCAT TTGGAGTATTGCTCTGGGAAATTGCTACCTATGGCATGTCACCTTACCCGGGAATTGACCTGTCTCAGGT TTATGAGCTGCTGGAAAAAGACTACCGCATGGAGCGCCCGGAAGGCTGCCCAGAGAAAGTCTACGAACTC ATGCGAGCATGTTGGCAGTGGAACCCCTCTGATCGGCCCTCCTTTGCTGAAATCCATCAAGCCTTTGAAA CCATGTTCCAGGAGTCCAGCATCTCAGACGAGGTGGAGAAGGAGCTGGGGAAACGAGGCATGAGAGGAGC CGCCGGGAGCACCCTGCAGGCCCCAGAGCTGCCCACCAAGACCAGAACCTGCAGGAAAACCGCTGAGCAG AAAGATGCACCTGACGCCCTGGAACTGCTCCACACGAAGGGCCTGGGCGAAAGCGACGCACTGGACAGTG AACCTGCTGTATCACCACTGCTTCCTCGGAAAGAGCGTGGGCCCCCGGACGGCAGCCTAAATGAAGATGA GCGTCTCCTCCCCAAAGACAAAAAGACCAACCTGTTCAGCGCCTTGATCAAGAAGAAGAAGAAAATGGCG CCGACGCCCCCTAAGCGCAGCAGTTCCTTCCGAGAGATGGATGGCCAGTCAGAACGCAGAGGGGCTAGTG AGGATGACGGCCGGGAAATCAGCAATGGGCCACCAGCTCTCACCTCAGATGCAGTAGAGCCTACCAAGTC CCCAAAGGCCAGCAATGGGGCTGGCGTCCCCAATGGAGCCTTCCGGGAGCCAGGCAACTCGGGCTTCCGC TCTCCCCATGTGTGGAAGAAGAGCAGCACACTGACCAGCAGCCGCCTCGCTGCTGCTGAAGAGGAGAGTG GCCTCAGTTCCAGTAAGCGCTTCCTGCGCTCCTGCTCGGCCTCCTGCATGCCCCATGGGGTTAGGGACAC TGAGTGGCGGTCAGTCACGCTGCCTCGGGACCTGCCGTCTGCTGGTAAGCAGTTTGACTCTTCCACCTTT GGAGGGCACAAAAGTGAAAAGCCAGCTCTGCCTCGGAAACGCACCAGTGAGAGCAGGTCTGAGCAGGTGG CCAAAAGCACAGCGACACCCCCTCCTCGGCTGGTGAAGAAGACTGAGGAGGCCGCCGAAGATGTCTTCAA AGACACGGAATCCAGCCCTGGCTCCAGCCCTCCCAGCTTGACCCCCAAACTCCTCCGTAGGCAGGTCCCT GCCTCCCCCTCCTCTGGCCCCTCTCACAAGGACGAGGCCACCAAGGGCAATGCCTCGGGCATGGGGACTC CTGCCACTGCAGAGCCAGCAGCCCCCAGCAACAAAGCGGGCCTCAGCAAGGCCTCCTCCGAGGAGCCCCG TGCAAGGAGACACAAGCACAGCTCGGAGTCCCCAGGGAGAGACAAGGGGCGGCTGTCCAAGCTCAAGCCG GCCCCGCCACCTCCGCCCTCCTCCACAGGGAAAGCAGGGAAGCCTGCACAGAGCCCCAGCCAAGATGTGG CTGGGGAGGCAGGTGGGGCCACCAAGACAAAATGCACAAGTCTGTCTGTGGATGCTGTGAACAGTGACCC CACCAAGGCTGGCCAGCCTGGAGAAGGACTGAGAAAATCTGTGCCCCCATCTGTGCCAAAGCCTCAGTCA GCTACTAAGCCACCGGGGACCCCCACCAGCCCGGTCTCCACCCCCTCCACAGCGCCAGCACCTTCACCCC TGGCTGGGGACCAGCAGCCATCTTCTGCTGCCTTCATCCCCCTCATATCAACCCGTGTGTCTCTTCGAAA GACCCGACAGCCGCCAGAGCGCATCGCCAGTGGCACCATCACCAAGGGTGTGGTTCTGGACAGTACTGAG GCCCTGTGCCTTGCCATCTCCCGGAACTCGGAGCAGATGGCCAGCCACAGTGCTGTGCTGGAGGCGGGCA AGAACCTGTACACATTCTGTGTGAGCTACGTGGACTCTATCCAGCAGATGAGGAACAAGTTTGCCTTCCG CGAGGCTATCAACAAGCTGGAGAGCAACCTCCGAGAGCTGCAGATCTGCCCTGCGACAGCCTCCAGTGGG CCAGCGGCCACACAAGACTTCAGCAAGCTGCTTAGCTCTGTGAAGGAGATCAGCGACATTGTCCGGAGGT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100850
Insert Size	3432 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100850.1, NP_001094320.1
RefSeq Size	6158 bp
RefSeq ORF	3432 bp
Locus ID	311860

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