Myrf (NM_001170487) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mrf; RGD1306622
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217501 representing NM_001170487 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGTGGACGAGACCGAAGCGCTGCAGCGCTTCTTCGAAGGCCACGACATCAGCGGTGCCCTGG AGCCCTCCAATATAGACACCAGTATCCTGGAGGAGTACATTGGCAAAGAGGACGCCTCTGACCTCTGCTT CCCTGAGATCTCTGCACCAGCCAGCACTGCCTCCTTCCCCCACGGGCCACCGGCCATTCCCGGCTCCAGC GGGCTCCACCATCTGAGCCCCCCTGGGAGCGGACCATCCCCTGGGCGCCATGGCCCCCTCCCACCCCCGA CCTACGGCACCCCACTCAACTGCAACAACAACAACGGCATGGGCACCGCCCCTAAGCCCTTCCTGGGGGG CTCTGGGCCTCCCATCAAGGCAGAGCCCAAGGCTCCCTATGCCCCAGGCACACTGCCAGACTCGCCCCCA GACTCAGGCTCTGAGGCCTACTCCCCTCAGCAGGTGAATGACCCCCATCTTCTGCGCACCATAACCCCGG AGACGCTCTGCCACGTGGGAGTTCCTTCCCGCCTGGAACACCCGCCCCCACCTCCAGCTCACTTGCCAGG CCCCCCGCCACCCCCGCCTCCCCCACCTCACTACCCTGTCCTGCAACGGGACATCTACATGAAGGCTGAG CCCCCTGTACCCCCTTATGCTGCCATGGGGCCGGGTTTGGTGCCCCCTGAGCTCCACCACACCCAGCAGA CCCAGGTGCTACAGCAGCTGCTGCAACAGCACGGAGCTGAGCTCCCTCCACACCCCTCTAAGAAGAGGAA GCACTCCGAATCACCCCCCAACACCCTCAATGCCCAAATACTGAATGGAATGATCAAGCAGGAACCTGGG ACTGTCACAGCCTTGCCTCCACACCCTGCCAGAGCCCCGTCCCCTCCGTGGCCTCAGGGCCCACTCTCAC CTGGCACTGGCTCCTTGCCCCTCAGCATTGCCCGAGCCCAGACTCCACCCTGGCACCCACCGGGTGCTCC CTCCCCAGGCCTCCTGCAGGACAGTGATAGCCTCAGTGGCTCCTATTTGGACCCCAACTACCAATCTATC AAATGGCAACCACATCAGCAGAACAAGTGGGCGACCCTATATGATGCCAACTACAAGGAGCTGCCTATGC TCACCTATCGTGTGGATGCAGACAAGGGCTTCAACTTTTCCGTGGGTGACGATGCTTTTGTGTGCCAGAA GAAGAACCACTTCCAGGTGACCGTGTACATTGGCATGCTGGGCGAGCCCAAGTATGTCAAGACGCCGGAA GGCCTCAAGCCCCTGGACTGCTTCTATCTGAAGCTGCATGGAGTGAAGCTAGAGGCCTTGAACCAGTCTA TCAACATCGAGCAGTCACAGTCAGACAGGAGCAAGAGGCCCTTCAACCCCGTCACGGTCAATCTTCCCCC TGAGCAGGTCACAAAGGTGACCGTGGGGCGACTCCATTTCAGTGAGACCACTGCCAACAACATGCGGAAG AAGGGCAAGCCCAACCCTGACCAGAGGTATTTCATGCTGGTGGTGGCTCTCCAGGCACATGCACAGAACC AGAACTACACACTGGCAGCCCAGATCTCAGAGCGCATCATTGTTCGGGCCTCTAACCCAGGCCAGTTTGA AAGTGACAGTGACGTGCTATGGCAACGGGCACAGCTGCCAGACACCGTCTTCCACCATGGCCGTGTGGGC ATCAACACCGACCGGCCAGATGAGGCACTGGTTGTCCACGGGAACGTCAAGGTGATGGGGTCGCTCATGC ACCCTTCAGATTTGCGGGCCAAGGAGCATGTGCAGGAGGTGGACACCACTGAACAGCTGAAGAGGATCTC TCGGATGCGGCTGGTACACTACAGATACAAGCCTGAGTTCGCTGCTAGTGCAGGCATTGAAGCTACCGCA CCGGAGACAGGTGTCATCGCCCAGGAAGTGAAGGAGATCCTGCCGGAAGCTGTGAAGGACACGGGGGATG TAGTCTTTGCCAATGGGAAAACCATAGAGAACTTCCTTGTAGTGAACAAGGAGCGGATCTTCATGGAGAA CGTGGGGGCTGTGAAGGAGTTGTGCAAGCTGACAGACAACCTGGAGACTCGCATTGATGAGCTGGAGCGC TGGAGCCACAAGCTGGCTAAGCTGCGGCGTCTCGACAGCCTCAAGTCAACTGGCAGCTCGGGCGCGTTCA GCCATGCGGGGAGCCAGTTTAGCCGGGCAGGCAGTGTCCCTCACAAAAAGAGGCCCCCTAAACTGGCCAA TAAGTCATCACCAGTGGTCCCAGATCAGGCCTGCATCAGTCAGCGCTTTCTGCAAGGAACTATCATTGCT CTGGTGGTGGTCATGGCCTTCAGCGTGGTGTCCATGTCCACACTATATGTGCTGAGCCTGCGCTCTGAAG AGGACCTGGTGGATGCTGACGGCTCTCTTGCCGTGTCCACTTCCTGTCTTCTGGCCCTGCTCCGGCCCCA GGACCCTGGGGGGAGTGTGGCCGTGTGCCCATGCAGGTCCAGCCAGAGCTTTGGGACCACACAGCTCCGA CAGTCCTCTATGACCACTGGACTACCAGGCACACAGCCGTCTTTGCTGCTGGTTACCAGTTCAGCCTCAG GTCCCGCTCTCCGTGCCTTGGACCTGTGCTCCAGCCAGCCCTGCCCCATTGTCTGCTGCTCTACTCCCGT CTCCAGTCCTGCTACAGACCCCAGCCTTGGCCCCATTGTTACTCCTACTCCAAGTCCCAGCCCTAGCCCC AACCCTAACCCCAAGCACTCAGGCCCTGGCCAGATGGCCCCTCTGCCAGTCACCAACATCAGAGCCAAAT CCTGGGGCATTTCAGCTAATGGCATCAGCTATTCCAAACATCCCAAGAGCCTGGAACCTCTAGCCAGTCC TGTGGTCCCCTTTCCTGGAGGGCAGAGCAAGACCAAAAACAGCCCCAGCTTCAGTCTCCAAAGTCGCGCC CGCAGAGGAGCCCCACAACCCAGCCCAAGCCCTGCCCAGTTCACTCAGACCCAGGGCCAGCTAGACCCAG TGCCATCCCTGACCTCCATCCAGCTGCTGGAGAATTCCATGCCCATCACCTCCCAGTACTGTGTGCCAGA GGATGCCTGCAGGCTTGGAAACTTCACCTACCACATCCCTGTCAGCAGCAGCACACCACTGCACCTCAGC CTGACCCTGCAGATGAATTCCTCCACCCCTGTGTCCGTGGTACTGTGCAGCCTGATTTCGAAGGAGGAGC CCTGTGAGGAGGGAGGCTTTTTGCAGACGCTCCACAGCCATCAGGACACCCAGGGCACCTCTCACCAGTG GCCAGTAACCATCCTGTCCTTCCGTGAATTCACATACCACTTCCGGGTGGCATTGCTGGGTCAGGCCGAC TGCAGCTCAGAGGCCGCCGTTCAGCCGGCCACAGACTACTACTTCCACTTCTACCGCCTGTGTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170487
Insert Size	3429 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170487.1, NP_001163958.1
RefSeq Size	5531 bp
RefSeq ORF	3429 bp
Locus ID	293736

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