Nemf (NM_001170586) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sdccag1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217449 representing NM_001170586 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACCCGCTTCAGCACTGTTGACCTCCGCGCGGTGCTCGCGGAACTGAATGCGAACTTGCTAGGAA TGAGAGTAAACAACGTTTATGATGTGGATAACAAGACGTATCTTATTCGGCTTCAAAATCCTGATTTTAA AGCTACACTGTTACTTGAATCTGGTATACGAATTCACACAACAGAATTTGAGTGGCCTAAGAACATGATG CCGTCTAGTTTTGCTATGAAGTGTCGAAAGCATTTGAAAAGTCGGAGATTAGTCAGTGCAAAACAGCTTG GTGTGGATAGAATTGTGGATTTCCAATTTGGAAGTGATGAAGCTGCTTATCACTTAATCATTGAGCTCTA TGACAGGGGCAACATTGTCCTTACAGATTATGAGTACCTAATTTTAAATATCCTAAGGTTTCGAACTGAT GAAGCTGATGATGTTAAGTTTGCTGTTCGTGAACGTTATCCCATAGATCATGCCAGAGCTGCTGAACCTT TGCTCACTTTGGAAAGATTGACTGAAGTAATAGCTAGAGCTCCTAGAGGTGAACTGCTGAAGAGAGTTCT GAATCCGTTACTTCCCTATGGGCCAGCTCTTATTGAGCATTGTCTTATAGAAAATGGATTCTCTGGCAAT GTCAAAGTGGACGAAAAACTTGAAAGCAAAGATATTGAGAAGATACTGGTTTGTGTGCAGAGGGCAGAAG ACTATTTGGAAAAAACAGCCAACTTCAATGGAAAGGGATATATAATTCAGAAAAGAGAGGTAAAACCAAG CCTGGATGCCAATAAACCAGCTGAAGACATACTCACGTATGAGGAATTTCATCCTTTCTTGTTTTCTCAA CATTTACAATGTCCATATATAGAATTCGAATCATTTGACAAGGCTGTGGATGAATTTTATTCCAAGATAG AAGGTCAGAAAATTGACTTAAAAGCATTGCAACAGGAAAAACAAGCACTGAAGAAATTAGATAATGTCCG AAAGGATCATGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATTGACAAACTGAAAGGGGAGCTC ATAGAGATGAACCTGCAGATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCATTAGCCAACCAGATAG ATTGGACAGAAATTGGAGTGATTGTGAAAGAAGCACAAGCCCAGGGAGACCCTGTTGCAAGTGCAATCAA AGAACTAAAACTGCAAACAAACCACATTACCATGCTTCTACGAAATCCATACTTGTTATCAGAGGAGGAA GATGGTGATGGTGATGGCAGCATTGAGAACAGTGATGCTGAAGCACCAAAAGGGAAAAAAAAAAAGCAAA AGAACAAGCAGCTGCAGAAGCCTCAGAAGAACAAGCCGCTGCTTGTCGATGTGGACCTCAGCCTGTCAGC CTATGCCAATGCCAAAAAGTATTATGATCATAAGAGGTATGCTGCTAAAAAAACACAGAGAACTGTCGAA GCTGCTGAGAAGGCATTCAAATCAGCAGAGAAGAAAACAAAGCAAACCTTAAAAGAAGTACAAACAGTTA CTTCTATCCAAAAAGCAAGGAAAGTGTATTGGTTTGAGAAATTTCTGTGGTTTATTAGTTCAGAGAACTA TCTCATTATAGGTGGTCGAGATCAGCAACAGAATGAGATTATTGTGAAAAGATACTTAACACCAGGAGAC ATTTATGTGCATGCTGATCTTCATGGAGCTACCAGCTGTGTAATTAAGAATCCAACAGGAGATCCCATCC CTCCTCGGACTTTGACTGAAGCAGGCACAATGGCACTTTGCTACAGCGCAGCTTGGGATGCCCGTGTTAT CACGAGTGCTTGGTGGGTGCACCATCATCAGGTCTCTAAAACAGCACCGACAGGAGAGTACTTAACAACT GGAAGCTTCATGATAAGAGGAAAAAAGAATTTCCTTCCTCCTTCATACCTAATGATGGGGTTTAGCTTCC TTTTTAAGGTAGATGAGTCCTGTGTCTGGAGACATCGAGGTGAACGAAAAGTCAGAGTGCAGGATGAAGA CATGGAAACATTGACAAGTTGCACAAGTGAACTCATGTCAGAAGAAATGGAACAGCTAGAAGGGGGCGAC AGCAGTGAAGAAGAGACCGAGGAGTTACGTGGAACCACGGAAGAAGTGGAACTCAGGACTCAGGCTGATC CAGAGGACATTGCTGCTCACAGTGGAAGAGAGGAACTGAGCTCTGAAGATGGAGAAGCCATCAAAACAGC CACGAAAGATGAAGAGCCCATTGGTGAGGTGAGGGAAGAGGAGGAAGAGCCCTTCGAATATCCTGACACC ACCATTGACTTGTCCCATCTTCAGTCCCAAAGGCCCCTACAGAAACTGACTCCAAGAGAAGAATCTTTTA ATTCAAGTGACAGTAAATCACAGAGCCGAAGACATTTGTCAGCCAAGGAGAGAAGAGAAATGAAAAAGAA AAAGCTCCCATGTGACTCAGGGGATTTAGAAGTGATAGAAGAAAAGGACAAAGAAAAAGAAAGTGCTGTG CACAGTGAAGCTGACCAGAACACAAGCAAAAATGTGGCAGCTGGACAGCCAATGAAAAGAGGCCAGAAGA GTAAAATGAAAAAAATGAAGGAAAAATACAAAGACCAGGATGATGAAGATCGTGAACTTATTATGAAATT GTTGGCATCTGCAGGTTCAAACAAAGAAGAAAAGGGGAAGAAAGGAAAGAAAGGAAAAACAAAAGATGAA CCCGTGAAAAAAAACCCACAGAAACCCAGAGGTGGACAGCGGGTTTTAGATGTTGTTAAAGAAACCCCGT CCCTTCAGGCGTCGACTCCTGACTTACAAGACTTTGCTGTGGATGAGCCACATGATGACAAGGAAGAACA TGATCTGGATCAGCAGGGAAATGAGGAAAATCTATTTGACTCTTTGACAGGGCAACCACATCCTGAAGAT GTATTAATGTTTGCTATTCCAATATGTGCTCCTTACACCATCATGACAAACTATAAATACAAAGTGAAAC TTACTCCTGGAGTTCAGAAAAAGGGAAAAGCTGCAAAGACAGCCTTGAACAGTTTCATGCACTCCAAAGA AGCAACAGCAAGAGAAAAAGACTTATTCCGAAGTGTGAAGGACACAGATTTATCAAGAAACATTCCCGGG AAAGTGAAAGTGTCTGCCCCCAATCTTCTGCACGTAAAAAGAAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170586
Insert Size	3198 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170586.1, NP_001164057.1
RefSeq Size	4797 bp
RefSeq ORF	3198 bp
Locus ID	100322884

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.