Stk10 (NM_019206) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217384 representing NM_019206 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCGCCAATTTTCGCCGCATCTTGCGGTTATCCACCTTCGAGAAGAGAAAGTCCCGTGAATACG AGCATGTCCGCAGGGACCTGGACCCCAACGACGTATGGGAGATCCTGGGCGAGCTGGGAGACGGCGCCTT CGGCAAAGTCTACAAGGCCAAAAACAAGGAGACGGGCGCCCTGGCAGCAGCCAAAGTCATTGAGACTAAG AGCGAGGAGGAGCTGGAGGACTACATCGTGGAGATCGAGATCCTCGCCACCTGCGACCACCCATATATCG TGAAGCTCCTGGGGGCCTATTACTATGACGGGAAACTATGGATCATGATTGAGTTCTGCCCTGGGGGAGC TGTGGATGCCATCATGCTAGAGCTGGATCGAGGCCTCACTGAGCCCCAGATTCAGGTCGTTTGCCGCCAG ATGTTGGAGGCTCTCAACTTCCTGCACGGCAAGAGAATCATCCACCGCGACCTGAAAGCTGGCAACGTGC TTATGACCCTGGAGGGGGACATCAGGCTGGCTGATTTTGGAGTATCTGCCAAGAATCTGAAAACTCTGCA AAAGCGAGATTCCTTCATAGGAACGCCTTACTGGATGGCCCCTGAGGTGGTGCTGTGTGAAACCATGAAG GACGCCCCCTATGACTACAAGGCTGACATCTGGTCCCTGGGCATCACGCTGATCGAGATGGCGCAGATTG AGCCCCCACACCATGAGCTCAACCCCATGCGGGTCCTGCTCAAGATCGCCAAGTCGGACCCTCCCACCCT GCTCACACCCTCCAAGTGGTCCACGGAGTTCCGAGACTTTCTGAAGATAGCCTTGGATAAGAACCCAGAA ACCCGGCCCAGTGCTGCGCAGCTGCTGCAGCATCCCTTCGTCAGTACAGTCACCAGTAATAAGGCCCTTC GGGAGCTGGTGGCTGAGGCGAAGGCTGAGGTGATGGAGGAGATCGAGGACGGCAAGGAGGACGGCAGGGA GGACGGCAGGGAGGATGGAGAAGAGGAGGATGGAGACGAGAAGGATGCTGTGAGTGCTGTTCCGCCCCCA GTCAACCACACTCAGGACTCCTCTGCCAATGGAACTCAGCCAAGCCTCAACTCTGACAAGCTTCTCCAGG ATTCTTCTACCCCCCTGCCTCCCAGCCAGCCTCAGGAGCCTGTGAACGGGCCCTGTAACCAACCCTCAGG GGATGGATCCCCCCAGAACACCAGCCCTGCAGATGAGGTCTCCAAGAATGACAATGGCTTAAAGGTACCT GTTCCCCTCCGGAAGTCCCGGCCATTGTCCGTGGATGCCAGAATTCAGGTGACCGAAGAGAAACAAATCA CTGACCAGGCTGAGAACCCCAGTTCTGCAGCCAGCAAACCCCCGAAGGTCAACCAGAGCCGACCTAACAG CAGCGCCCTGGAGACTTTGGGTGTCGAGACTCTGGCCAATGGAGGCCTGGAGCTCCCTGGCTCTGTAACT CCAAACCATTCTAAGAGGGCGTCGGACTGTAGCAACCTGTCTACCTCAGAGAGCATGGACTACGGCACCT CCTTGTCTGCTGACCTGTCATTGAACAAAGAGACGGGCTCATTGTCTCTCAAGGGCTCAAAACTGCACAA CAAGACCCTGAAGAGGACCCGCCGGTTTGTGGTGGACGGTGTGGAGGTGAGCATCACCACCTCCAAGATC ATCAGCGAAGACGAGAAGAAAGACGAGGAGATGAGGTTTCTCAGGCGCCAGGAACTCCGAGAGCTTCGGC TCCTGCAGAAAGAAGAGCATCGGAACCAGACGCAGCTGAGCACCAAGCACGAGCTGCAGCTGGAGCAGAT GCACAGACGATTTGAACAAGAAATCAACGCCAAGAAGAAATTCTATGACGTGGAGCTAGAGAACCTGGAG CGGCAGCAGAAGCAGCAGGTGGAGAAGATGGAGCAGGACCACAGCGTGCGTCGCAGAGAGGAGGCCAAGC GGATCCGCCTGGAGCAGGATCGAGACTACGCCAGGTTCCAAGAGCAGCTCAAGCAGATGAAGAAGGAGGT GAAGCTGACTGTCGAGAGGTTGGCTAGAAATAGTAGAAATAGTTCTTCCTTCCAGGAACAGACTGAGCAT CCTCAGGCAGCTAGGAGGAGGGACCGAGACTTTGTAGCCAAGCAGAAGGAAGACCTGGAGCTGGCCATGA AGAAGCTCACGGCAGAAAACAGGCGTGAGATCTGTGACAAGGAACGTGATTGCCTTAACAAGAAGCAGGA GCTCCTCCGAGACCGAGAGGCAGCCCTGTGGGAGATGGAGGAGCACCAGTTACAGGAGAGACATCAGCTG GTGAAGCAGCAGCTTAAGGACCAGTACTTCCTGCAGCGGCATGACCTGCTGCGCAAGCACGAGAAGGAGC GGGAGCAGATGCAGCGCTACAACCAGCGTATGATGGAGCAGCTGAAGGTCAGACAGCAGCAGGAGAAGGC GCGGCTACCCAAGATCCAGAGGAGTGACGGCAAGACCCGCATGGCCATGTACAAGAAGAGCCTGCACATC AATGGTGCGGGCAGTGCCTCCGAGCAGCGGGAGAAGGTCAAGCAGTTCTCCCAGCAGGAAGAGAAGAGGC AGAAGGCGGAGAGGCTGCAGCAGCAGCAGAAACACGAGAACCAGATGCGAGACATGGTGGCACAGTGCGA GAGCAACATGAACGAGCTGCAGCAGCTGCAGAATGAAAAGTGTCATCTGTTAGTGGAGCATGAAACCCAG AAGCTGAAGGCCCTGGACGAGAGCCATAACCAGAGCCTGAAGGAATGGCGAGACAAGCTTCGGCCACGCA AAAAGGCCCTGGAAGAGGATTTGAACCAGAAGAAGCGGGAACAGGAAATGTTCTTCAGACTAAGTGAGGA GGCAGAGACCAGACCCACCACACCCAACAGAGCCAGCAAGTTCTTCCCCTACAGCTCTGGGGATGCTTCC TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019206
Insert Size	2943 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019206.2, NP_062079.2
RefSeq Size	5153 bp
RefSeq ORF	2943 bp
Locus ID	29398
Gene Summary	an astrocyte gene which utilizes type 1 astrocyte-enriched cDNA library [RGD, Feb 2006]

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