Dlgap3 (NM_001276304) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	DAP-3; Dap3; SAPAP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217375 representing NM_001276304 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGTTACCATGGCGACCGAGGCAGCCATCCCCGCCCAGCCCGCTTTGCTGACCAACAGCATATGG ACGTGGGCCCAGCTGCCAGGGCCCCGTACCTGCTGGGCTCCAGGGAGGCCTTCTCCACCGAGCCCCGCTT CTGTGCCCCGAGAGCTGGCCTGGGACACCTTTCTCCAGAAGGGCCCCTGAGCCTGAGTGAGGGTCCATCA TCTGTAGGCCCCGAGGGAGGCCCAGGGGGGGTGGGGGCTGGGGGAGGCAGCAGTACCTTCCCCAGGATGT ACCCGGGCCAGGGCCCCTTCGACACCTGTGAAGACTGTGTGGGCCACCCACAGGGCAAGGGTGCCACCCG CCTGCCTCCCACACTCCTGGATCAGTTTGAAAAGCAGTTGCCAGTTCAACAAGATGGCTTCCACACACTC CCATACCAGCGAGGTCCAGCAGGGCCTGGTCCTGGACCTGGATCTGGCGCTGCCCCTGAGGCTCGCAGCG AGAGTCCTAGCCGCATCCGGCACCTGGTTCACTCTGTGCAGAAGCTCTTTGCCAAGTCCCACTCTCTGGA GGCACCTGGGAAGCGAGACTATAATGGGCCTAAGGCCGAGGGAAGAAGTAGCTCTGGGGGAGACAGCTAC TCTGGTCCAGGCTCAGGAGGCCCCCCCACCTCCCACCACCACCATCACCACCACCACCATCACCACCACC AGTCCCGCCATGGCAAACGGAGCAAGAGCAAGGACCGCAAGGGGGATGGGCGACACCAGACCAAGGCCAC AGGCTGGTGGAGCTCTGATGACAATTTGGACAGTGACAGTGGCTTTCTGGGTGGGAGGCCCCCTGGAGAG CCCGGAGGTCCCTTCTGCCTGGATGCTCCAGATGGGTCCTACCGGGACTTGAGCTTCAAGGGGCGTTCGG GCGGGTCAGAAGGCCGCTGCCTTGCCTGCACTGGCATGTCCATGTCCCTGGATGGACAGTCAGTCAAGCG AAGTGCCTGGCATACCATGATGGTCAGCCAGGGCCGGGATGGATACCCAGGGGCCGGGCCAGGCAAGGGG CTCCTGGGTCCAGAGACCAAGGCCAAAGCCAGGACTTATCACTATTTGCAGGTGCCGCAAGATGACTGGG GGGGTTACCCCACGGGTGGCAAGGATGGGGAGATCCCCTGCCGCAGGATGCGGAGCGGCAGCTACATCAA AGCCATGGGGGACGAGGAGAGCGGAGACTCAGATGGTAGCCCCAAGACGTCTCCCAAAGCACTTGCCCGA CGCTTTGCCTCCCGCCGTTCCTCCAGCGTGGACACAGCCAGGATCAACTGCTGTGTCCCACCCCGGATCC ATCCCCGGAGTTCCATCCCTGGCTACAGCCGTTCCCTTACAACTGGACAGCTAAGCGAAGAGTTTAACCA GCAGCTGGAGGCCGTGTGCGGGTCCGTGTTTGGGGAGCTTGAGTCCCAGGCCGTGGACGCCCTGGACCTG CCCGGCTGTTTCCGCATGCGGAGCCACAGCTACCTCCGGGCCATCCAGGCCGGCTGCTCTCAAGACGACG ACTGCCTACCCCTCCTTGCTGCCCCTGCCTCTGTCTCAGGGAGGCCCGGCTCCTCCTTCAACTTCAGAAA GGCCCCGCCCCCTATCCCGCCGGGAAGCCAGGCCCCACCCCGCATCTCCATCACCGCCCAGAGCAGCACC GACTCTGCCCACGAGAGCTTCACTGCAGCAGAGGGCCCGGCCCGGCGCTGCAGCTCCGCGGACGGACTGG ACGGCCCCACCATGGGCGCACGCACCCTCGAGTTGGCACCGGTGCCACCCCGGGCCAGCCCCAAGCCCCC CACACTCATCATCAAGACCATTCCTGGCCGGGAGGAGCTGCGGAGCCTGGCGCGACAGCGGAAGTGGCGG CCGTCCATTGGGGTGCAGGTGGAGACAATCTCAGACTCGGACACCGAGAACAGGAGCCGAAGAGAGTTCC ACTCCATTGGCGTTCAGGTGGAAGAGGACAAGAGGCGGGCGAGGTTCAAGCGCTCCAACAGTGTGACAGC CGGTGTGCAGGCAGACCTGGAACTGGAGGGCCTGGCTGGCCTGGCCACTGTGGCCACAGAAGACAAAGCC CTGCAGTTCGGACGCTCCTTCCAGAGGCACGCCTCTGAGCCCCAGCCCGGACCCCGGGCCCCCACCTACT CAGTCTTCCGCACGGTCCACACGCAGGGCCAGTGGGCCTACCGCGAGGGCTACCCACTGCCGTATGAGCC GCCGGCCACCGATGGGTCGCCTGGCCCTCCCCCTGTCCCCGCCCCCGGCCCCGGGTCCGGCCGCCGTGAT TCCTGGATGGAGCGGGGTTCGCGTAGCCTCCCCGACTCAGGCCGCACGTCCCCCTGCCCACGGGATGGCG AGTGGTTCATCAAGATGCTGCGGGCAGAGGTGGAGAAACTGGAACACTGGTGCCAGCAGATGGAGCGGGA GGCGGAGGACTATGAGCTGCCGGAGGAGATCTTAGAGAAGATTCGAAGCGCCGTGGGCAGCACACAACTT CTCCTGTCTCAGAAGGTCCAGCAGTTCTTCAGGCTGTGTCAGCAAAGCTTGGACCTGGCCGGTTTCTGGG ACCTCCTGCAGCTCTCCATTGAGGATGTGACCCTCAAGTTCCTGGAGCTACAACAACTCAAGGCCAACAG CTGGAAACTCCTGGAGCCTAAGGAGGAGAAGAAGGTCCCTCCGCCGATACCAAAGAAGCCCTCGCGGGGG CGGGGCGTGCCGGTGAAGGAGCGCTCCCTGGACTCGGTGGACCGGCAGCGGCAGGAAGCGCGCAAGCGGC TCCTGGCGGCCAAGCGCGCCGCCTCGTTCCGCCACAGTTCGGCTACTGAGAGCGCGGACAGCATCGAGAT CTACATCCCCGAGGCCCAGACCAGGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276304
Insert Size	2901 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276304.1, NP_001263233.1
RefSeq Size	3688 bp
RefSeq ORF	2901 bp
Locus ID	286923
Gene Summary	binds neuronal cell guanylate kinase PSD-95/SAP90; may play a role in post synaptic density structure and synaptic function [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The resulting isoform (2) is shorter compared to isoform 1. The 5' UTR and 5' coding region for variant 2 are identical to those of variant 1 so the synthesis of two isoforms based on alternate in-frame translation starts discussed in PMID: 227619920 for variant 1 may also occur. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.