Map3k10 (NM_001191621) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217351 representing NM_001191621 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGAGGAGGAGGAGGGGGCGGCGGCCAGAGAGTGGGGCGCGACCCCCGCGGGGCCAGTGTGGACCGCCG TGTTCGACTACGAGGCGGTGGGCGACGAGGAGCTGACCCTGCGGAGAGGCGATCGCGTCCAGGTGCTTTC CCAGGACTGTGCGGTGTCTGGAGATGAGGGCTGGTGGACAGGGCAGCTGCCCAGCGGCCGAGTGGGCGTC TTCCCCAGCAACTACGTGGCCCCCGCTGCACCCGCTGCACCCACAGACCTCCAGCTGCCACAGGAGATCC CCTTTCACGAGCTGCAGCTGGAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTACCGCGCCTTATG GCGTGGAGAGGAAGTAGCGGTCAAGGCCGCCCGGCTGGACCCTGAACGGGACCCAGCAGTGACAGCGGAG CAGGTGCGCCAGGAGGCCCGGCTCTTTGGAGCCCTTCAGCACCCCAACATCATTGCCCTGAGGGGCGCCT GCCTCAGCCCCCCAAACCTCTGCCTGGTGATGGAATATGCTCGGGGAGGTGCATTGAGCAGGGTACTGGC AGGTCGCCGGGTCCCCCCTCATGTGCTGGTCAACTGGGCTGTGCAGGTGGCACGGGGCATGAACTATCTG CACAATGATGCCCCTGTACCCATCATTCACCGGGACCTCAAGTCCATCAACATCCTAATTCTGGAGGCCA TTGAGAACCACAACCTCGCTGACACGGTGCTCAAGATCACAGACTTCGGCCTTGCCCGAGAGTGGCACAA GACGACCAAAATGAGTGCGGCGGGGACCTACGCCTGGATGGCTCCTGAGGTTATCCGCCTCTCCCTCTTC TCCAAAAGCAGCGATGTCTGGAGCTTCGGGGTTCTTCTTTGGGAGCTGCTGACCGGGGAGGTCCCCTACC GAGAAATCGACGCCTTGGCCGTGGCTTACGGTGTGGCTATGAACAAGCTGACGCTACCCATCCCCTCCAC GTGCCCCGAGCCCTTTGCCCGCCTACTGGAGGAATGCTGGGACCCAGACCCTCATGGGCGACCAGATTTT GGCAGCATCTTGAAGCAGCTGGAGGTCATCGAGCAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCC ACTCACTGCAGGAAGACTGGAAGCTGGAGATTCAGCATATGTTCGATGACCTTCGGACCAAGGAGAAGGA ATTGCGCAGCCGTGAAGAAGAGCTGTTGCGGGCCGCACAGGAGCAGCGTTTTCAGGAAGAGCAGCTGCGG CGGCGGGAGCAGGAGCTGGCCGAGCGAGAGATGGACATTGTAGAACGTGAACTGCACCTGCTCATGAGCC AGCTGAGCCAGGAGAAGCCCAGGGTCCGCAAACGCAAGGGCAACTTCAAACGCAGCCGCTTGCTCAAGCT ACGGGAAGGCAGCAGCCACATCAGCCTGCCCTCCGGATTTGAACACAAGATCACTGTCCAGGCCTCCCCC ACCCTGGACAAGAGGAAAGGATCTGATGGGGCCAGCCCTCCTGCCAGCCCCAGCATCATTCCCCGGCTGA GGGCCATTCGCTTGACCCCCGTAGACTGTGGCAGCAGCGGTGGCAGTGGAACATGGAGCCGCAGTGGGCC ACCAAAAAAGGAAGAACTGGTTGGGGGCAAGAAGAAAGGCAGGACGTGGGGTCCCAGTTCTACTTTACAA AAGGAGCGGGCCGGAGGAGAGGAGAGGCTCAAGGCCCTGGGGGAAGGAAGCAAGCAGTGGTCCTCGAGCG CCCCCAACCTGGGCAAATCCCCTAAGCACACCCCCATGGCCCCAGGCTTCGCCAGCCTCAATGAGATGGA AGAATTTGCGGAGGCAGATGAAGGCAACAACGTGCCCCCTTCCCCCTACTCCACCCCGTCCTACCTCAAG GTGCCTCTGCCCGCCGAGGCCTCTCCTTGTGCTCAGCCGCCTTGGGAGCCGCCGGCTGCGACCCCCTCCC GGCCGGGACACGGAGCTCGGAGGCGCTGTGATCTGGCTCTGCTGGGCTGCGCCACGCTGCTGAGTGCGGT GGGCCTGGGCGCCGACGTTGCCGAGGCACGGGCCGGGGATGGTGAGGAGCAGCGGGGCTGGCTGGACGGT CTCTTCTTCCCACGGCCCGGCCGCTTCCCTCGGGGTCTCAGCCCGACAGGGCGCCCAGGAGGTCGCCGCG AAGAGACAGCCCCAGGCTTTGGCCTGGCACCCTCGGCCACCCTGGTGTCTTTATCTTCTGTATCTGATTG CAACTCTACGCGCTCATTGCTGCGCTCTGACAGTGATGAGGCTGCCCCCACTGCGCCCTCCCCGCCGCCT TCTCTGCTCCCGCCCTCACCTAGCACCAATCCTCTTGTGGACGTAGAGCTGGAGAGCTTCAAGAAGGACC CCCGGCAGTCGCTCACGCCCACCCACGTCACTGCGGCCCACGCTGTGAGCCGTGGACACAGACGGACACC ATCGGATGGGGCTCTGAGACAGCGGGAGCCTCCAGAGCTCACCAACCACGGCCCAGGTCCTCGAGATCCC TTGGACTTCCCTCGACTGCCCGACCCCCAGGCCCTGTTCCCTACCCGACGCCGGCCCCTGGAGTTCCCTG GCCGTCCCACCACCCTCACCTTTGCCCCAAGACCCCGGCCAGCTGCCAGCCGCCCCCGTCTGGACCCCTG GAAACTGGTCTCCTTTGGCCGGACACTCAGCATCTCGCCTCCAAGCAGGCCAGACACTCCAGAGAGCCCT GGGCCCCTCAGCGTGCAGCCCACGCTGCTGGACATGGACATGGAGGGTCAGAGCCAGGACAATACAGTGC CCCTGTGTGGGGCCTATGGCTCTCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001191621
Insert Size	2829 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191621.3, NP_001178550.1
RefSeq Size	3447 bp
RefSeq ORF	2829 bp
Locus ID	308463
Gene Summary	Activates the JUN N-terminal pathway.[UniProtKB/Swiss-Prot Function]

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