Ect2 (NM_001108547) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217314 representing NM_001108547 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGACAGTGTGTTACCATCTCCTACTGAGATAACTAGCTTGGCAGACACTTCAGTTTTTGATT CTAAAGTTACAGAAACATCCAAGGAAAACTTGTGCATGGTGTCTACTTCAAATGTTGACGAAGAAATGCC TCAGGTGGAAGCAAGAGTGGTAGTGGTCCAGGAAGCTGGGAAACAAGAGGAGCTCCTAAAGGCCTTAAAG ACTGTTAAGATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGGAAATCGGATGAAAAGT TAATAAAAAGTATTATTAATATGGAAATTAAAGTGCCCTGTGTAAAGATGGATTCAATGGAAGAATTTGG AAGTTTGGATTCCCCAGAATTTGAGAATATATTTGTAGTTACTGACTTCCAGACATCTGTCTTCAATGAC TTATACAAGGCTGATTGTAGGATTGTTGGACCACCAGTTATACTCAACTGTGCACAAATGGGAGAGCCCT TGCCGTTCTCCTGCCGGCCGCTGTACTGCACGAGCATGCTGAATCTGGTGCTCTGCTTCACTGGATTCAG GAAGAAGGAGGAGCTCGTCAAATTGGTGACATTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAATGC AATTCCAAAGTAACACATCTGGTGGCAAATTGTACGCAAGGAGAAAAGTTTAGGGTTGCTGTGAGCCTGG GGACTCCAATTATGAGGCCAGGATGGATTTATGAAGCATGGGAAAGACGCAATGAACAGTGTTTCTGTGC AGCAGATGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTTCTGGGA TTTTCAGATGAAGAGAAACACAGTATGGAAGAAATGACTGAAATGCAAGGAGGTACCTATTTACCAGTTG GGGATGAAAGGTGCACTCACCTTATTGTTGATGAGAACACAGTAAAGGAACTTCCATTTGAACCTTCCAA GAAACTTTTTGTGGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAGATGGATGCCCGGGCTGGTGAAACT ATGTATTTGTATGAAAAGGCTAATACTCCTGAGCTCAAGAAATCCGTGTCTTTGCTGTCTCTGAGTACTC CAAACAGCAACCGCAAAAGACGGAGGTTGAAAGAAAGCCTGGCTCAGCTCTCCAGAGAGACCGACCTCTC TCCTTTCCCTCCGCGCAAGCGGCCCTCAGCAGAGCATTCCCTTTCTATCGGCTCGCTCCTGGATATCTCC AACACACCGGACTCCAGCATTCACTATGGAGAAACACCGAAGTCTTGTGCTAAGTCTTCCAGGAGTTCAA CTCCAGTTCCTCCAAAGCAGTCGGCCAGGTGGCAGGTGGCAAAAGAGCTCTACCAGACTGAGAGCAACTA TGTCAATATACTGGCCACCATCATTCAATTATTTCAGGTACCCTTAGAAGAAGAAGGACAACGGGGTGGG CCTATTCTTGCACCCGAGGAAATCAAGACTATTTTTGGGAGCATCCCAGACATCTTTGACGTACATATGA AGATAAAGGATGATCTTGAAGACCTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCT TAAATATTCCAAAGATTTGGTAAAAACCTATCCTCCATTTGTAAACTTCTTTGAAATGAGCAAGGAAATG ATTATTAAATGTGAAAAACAGAAGCCCAGATTTCATGCTTTTCTCAAGATAAATCAAGCTAAACCAGAGT GTGGACGGCAAAGCCTTGTGGAACTTCTCATTCGGCCAGTACAGCGGTTACCCAGTGTTGCCTTACTTTT AAACGATCTTAAAAAGCATACAGCTGATGAAAATCCAGACAAAAGCACGTTAGAAAAAGCTATTGGATCA CTAAAAGAAGTAATGACACATATCAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATG TTGTTTATGAAGTAGATGGATGCCCAGCTAATCTATTATCTTCTCATCGAAGCCTGATACAAAGAGTGGA AACAGTTTCTCTTGGTGAGCACCCCTGTGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGC CTTGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGAAGTCCTCATGACCGCACCCGGCCAC CAGCTTCTCTGAAGCACATTCATCTAATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCAGAGAGAC AGAAGATTGTCATAATGCCTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCCAACGTGCTGCTCAGC TTCCAGATGACGTCAGAGGAGCTTCCAAAGGAGAGCTGGCTGAAGATGCTGTGTCGACATGTTGCCAACA CCATTTGTAAAGCAGATGCTGAGAATCTTATGTACGTGACTGATCCAGAATCTTTTGAAGTAAATACAAA AGATATGGATAGTACACTTAGTCGAGCATCTAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGGGCA TTCTCTTTTTCCAAAACTCCCAAGAGAGCCCTGCGGATGGCTCTGTCATCATCCCATAGCTCAGAGGGAA GGAGTCCTCCGAGTAGTGGCAAACTTGCTGTGAGCCGTCTGTCCAGCACATCTTCCTTAGCAGGTATTCC ATCGCCTTCCCTTGTCAGCCTCCCTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACT CACTTGATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108547
Insert Size	2742 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108547.2, NP_001102017.2
RefSeq Size	4043 bp
RefSeq ORF	2742 bp
Locus ID	361921

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.