Gria2 (NM_001083811) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluA2; gluR-B; GluR-K2; GluR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217297 representing NM_001083811 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGAGTCTACGCAATTTTTGGATTTTATGACA AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTCCATGTGTCCTTCATCACACCTAGCTTCCC AACAGATGGCACACATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT GAGTACTACCAATGGGACAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG TTCTGGATTCTGCTGCAGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATCAACAATGACAA GAAAGATGAGACCTACAGATCGCTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTGGAC TGTGAAAGGGATAAAGTAAATGACATTGTGGACCAGGTTATTACCATTGGAAAACATGTTAAAGGGTACC ATTATATCATTGCAAATCTGGGATTCACTGATGGGGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT CTCTGGATTTCAGATTGTAGACTACGACGATTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTG GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACGTATGATGCTG TCCAAGTGATGACTGAAGCATTCCGTAACCTTCGGAAGCAGAGGATTGAAATATCCCGGAGAGGAAATGC AGGAGATTGTTTGGCCAACCCAGCTGTGCCCTGGGGACAAGGGGTCGAAATAGAAAGGGCCCTCAAGCAG GTTCAAGTTGAAGGCCTCTCTGGAAATATAAAGTTTGACCAGAATGGAAAACGAATAAACTACACAATTA ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT CACCCTAACTGAGCTCCCATCAGGAAATGACACGTCTGGGCTTGAAAACAAGACTGTGGTGGTCACCACA ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTACGAGG GCTACTGTGTTGACTTAGCTGCAGAAATTGCTAAACACTGTGGGTTCAAGTACAAGCTGACTATTGTTGG GGATGGCAAGTATGGGGCCAGGGATGCCGACACCAAAATTTGGAATGGTATGGTTGGAGAGCTTGTCTAC GGGAAAGCTGACATTGCAATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCCA AGCCCTTCATGAGTCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG GCAAGGATGCGATATTTCGCCAAGATCCCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC CTGATCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCTCCCA TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA AGAGTTTTTCAGGAGATCTAAAATCGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC TCTGTGTTTGTGAGGACTACCGCAGAAGGAGTAGCCAGAGTCCGGAAATCCAAAGGAAAGTATGCCTACT TGCTGGAGTCCACAATGAACGAGTACATCGAGCAGAGGAAGCCTTGTGACACCATGAAAGTGGGAGGAAA CTTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAGGAAATGCGGTTAACCTCGCA GTACTAAAACTGAATGAACAAGGCCTGTTGGACAAATTGAAAAACAAATGGTGGTACGACAAAGGAGAGT GCGGCAGCGGGGGAGGTGATTCCAAGGAAAAGACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATT CTACATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATCCACAGAATATTAACCCATCTTCCTCGCAGAATTCCC AGAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAGAGTGTTAAAATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001083811
Insert Size	2652 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001083811.1, NP_001077280.1
RefSeq Size	6745 bp
RefSeq ORF	2652 bp
Locus ID	29627
UniProt ID	P19491
Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate exon in the 3' coding region compared to transcript variant 1, and encodes an isoform (2, also known as the flop isoform) that is the same length as isoform 1, but with few amino acid differences. RNA editing changes Gln607Arg (CAG->CGG) and Arg764Gly (AGA->GGA). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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