Kif2b (NM_001005874) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217073 representing NM_001005874 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCAGTTCTGCCTTCCTCTGGCCCCACGCCTCTCACCCCTTAAACCTCTGAAATCCCACTTCA CAGACTTCCAGGTGGGCATCTGTGTGGCGATCCAGCGCAGTGACAAACGGATTCATCTGGCTGTGGTCAC AGAGATCAACCGAGAAAACTCCTGGGTCACGGTAGAGTGGGTTGAGAAAGGAGTCAAAAAAGGAAAGAAG ATTGAACTGGAGACTGTACTTCTGTTGAACCCTGCTTTGGCTTCTACTGAACACCAACGGTCACGTAGAC CCCTGCGCCCAGTGTCTGTAATACCCGCTACTGCCATTGGAGACCAGCGAACAGCCACCAAATGGATTGC GATGATTCCACACAGAAATGAAACACCCTCAGGGGACAGCCAAACCCTGGTGATCCCCAGCAACCCTTGT CTGATGAAGCGGAAAAAATCCCCCTGCCTACGGGAAATTGAAAAGCTGCAGAAGCAGAGAGAGAAGCGCC GACGACTGCAGCTAGAGATCCGAGCTAGACGAGCCCTTGACATCAACACTGGAAATCCAAACTTTGAGAC TATGCGCATGATTGAGGAGTACCGCAGGCATCTAGACAGCAGCAAGATGTCCCGCCTGGAGCCCCCAGAA GACCACCGCATCTGTGTGTGTGTGAGGAAGCGCCCTCTTAATGAGAGGGAGACCACCATGAAGGACTTAG ATATCATCACTATTCCCTCGCACAATGTGGTCATGGTACACGAGTCTAAACAAAAGGTGGACCTGACCCG CTACTTGGAAAACCAGACCTTCTGTTTTGACCATGCCTTTGATGACACGGCATCTAATGAATTAGTTTAC CAGTTCACCGCCCGGCCTCTTGTGGAGTCCATCTTCCGGAAGGGCATGGCCACTTGCTTTGCCTATGGGC AGACTGGCAGTGGAAAAACCCACACAATGGGTGGAGCCTTTTCAGGAAAGGCCCAAGAGTGTTCTAAAGG GATTTATGCCCTGGTAGCTCAGGATGTCTTCCTCCTCCTAAGAACCCCTGCCTATGAGAAATTGGAGCTC AAAGTCTACGGGACCTTTTTTGAGATCTATGGTGGCAAAGTGTACGATCTGCTAAACTGGAAGAAGAAGC TGCAAGTCCTGGAGGATGGAAATCAGCAAATACAAGTGGTAGGACTGCAGGAGCAGGAGGTGGGCTGTGT GGAGGAAGTCCTGAATCTGGTGGAGCTCGGAAACAGCTGTCGGACTTCCGGTCAGACCTCTGTCAACGCC CACTCATCCAGGAGCCACGCTGTGTTCCAGCTCATTCTCAAGTGCGGAGGGAAACTGCACGGCAAGTTTT CCCTCGTTGACTTAGCTGGGAACGAAAGGGGAGCAGATACTGCCAAGGCTACCCGGAAGAGGCAGCTGGA AGGGGCTGAGATAAATAAAAGTCTGCTAGCTCTAAAAGAGTGCATCAGGGCTTTGGGTAAGAACAAGTCT CATACCCCATTCAGAGCGAGCAAACTCACACAGGTACTCCGGGACTCCTTCATAGGGCAGAACTCCTATA CTTGCATGATCGCTACAATCTCGCCCGGGATGACTTCCTGTGAAAACACCCTCAATACTTTAAGATATGC AAACAGAGTCAAAGAATTGGCTTTAGAAGCAAGGCCTTACCCTCCAACTGACCATGAAATGCCACTAACG TTAGAAAACGGCAATACAAATTCGGAAAAGTCCCTTCAGAAGGATGACATCATTCAAATACCAACTGTAC AGAAAGAGGAAGAGAAAGAAAGCGATGAACTCACATCAACAAAGGAGCCAGCAGCTTCATGGAGCAGGTC CGGTCCATGGTGGGAGGCCATCCAGGAAACGGCTGAGGGAGTTAACTGTGATGTGGATTTTTGCATTGCG CAGTCTTTGTCCATTTTGGAGCAGAAAATTGGTGTGCTGACTGAGATCCAAAAGAAGCTCCAATTGCTAC GAGATGACCTCCAAAAGAAAAGCCAAGCTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001005874
Insert Size	1995 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001005874.2, NP_001005874.2
RefSeq Size	2240 bp
RefSeq ORF	1995 bp
Locus ID	287624
UniProt ID	Q5XI51
Gene Summary	Plus end-directed microtubule-dependent motor required for spindle assembly and chromosome movement. Has microtubule depolymerization activity. Plays a role in chromosome congression.[UniProtKB/Swiss-Prot Function]

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