Txnrd3 (NM_001106609) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217002 representing NM_001106609 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCGCCACCCGGCCGCCGCGCCCGCCTGACGTCCCCCGGGACCAGCCGCCCGCCCGCTGAGGTCC GCGAGGAGGTGCGGCGCCGCCTGCGGGATCTCATCGAGGGCAACAGGGTTATGATCTTCAGCAAGAGTTA CTGTCCACACAGCTCGCGGGTTAAGGAACTCTTTTCATCCTTGGGAGTGAACTATTACATCCTGGAACTT GATCAAGTTGATGATGGGGCCAATGTTCAGGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTGCCCA ATATTTTTGTGAATAAAGTGCACGTGGGTGGATGTGACCGGATTTTCCAGGCACATCAGAATGGTTTACT GCAGAAGCTCCTTCAGGATGACTCAACTCATGATTATGATCTCATCGTCATCGGCGGGGGCTCTGGCGGC CTCTCTTGTGCCAAGGAAGCTGCCAACTTGGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTTCCCTCGC CTCAGGGCACGTCCTGGGGCCTTGGCGGCACCTGTGTGAATGTAGGCTGTATCCCTAAGAAGCTGATGCA TCAGGCAGCCCTCCTGGGGCATGCTCTGCAAGATGCGAGGAAATATGGCTGGGATTATAACCAGCAGGTG AAGCACAACTGGGAGACCATGAGGGAAGCGATCCAGAACCACATTGGCTCCTTGAACTGGGGCTACAGGG TAACTCTTCGGGAGAAAGGCGTGACCTATGTCAACTCCTTCGGGGAGTTTGTGGAACTGCATAAAATAAA GGCAACCAATAAGAAAGGGCAGGAAACTTTTTACACCGCTTCGAAGTTTGTCATAGCAACAGGGGAAAGG CCGCGGTACTTGGGAATCCAGGGAGATAAAGAGTACTGTATTACCAGCGACGACCTGTTCTCTCTGCCAT ACTGCCCAGGGAACACATTAATCGTTGGCGCCTCGTATGTTGGTCTGGAGTGTGCAGGCTTTTTGGCTGG CTTGGGGCTAGATGTGACAGTCATGGTACGCTCTGTCCTTCTTCGTGGCTTTGATCAAGAAATGGCAGAG AAAGTGGGATCCTACCTGGAACAACAAGGCGTCAAGTTCCAAAGGAAATTCACCCCCATTTTGGTTCAAC AGTTGGAGAAAGGCTTACCCGGAAGATTGAAAGTCGTGGCTAAGTCCACTGAAGGACCAGAGACAGTAGA AGGGACATACAACACGGTTTTGTTAGCAATTGGTCGTGACTCCTGTACAAGGAAAATAGGGCTGGAGAAG ATCGGGGTCAAAATCAATGAGAAGAATGGCAAAATACCAGTAAATGATGTGGAACAGACCAATGTGCCTC ATGTTTATGCTATTGGGGACGTACTGGAGGGCAAACCAGAGCTCACACCTGTTGCCATACAGGCAGGCAA GCTGCTAGCTCGAAGACTCTTTGGGATCTCTTTAGAAAAGTGTGATTACATTAACGTCCCAACAACGGTG TTCACACCTCTGGAATACGGCTGTTGTGGACTGTCTGAAGAGAAAGCCATTGAAATGTATACGAAGGAGA ATCTGGAAGTGTATCACACCTTCTTTTGGCCTCTTGAGTGGACAGTTGCTGGCAGAGACAACAACACCTG TTATGCAAAGATAATCTGCAACAAATTTGACAATGACCGTGTGATAGGATTTCACCTCCTGGGGCCAAAT GCTGGTGAAGTCACACAGGGGTTTGCAGCTGCAATGAAATGTGGGCTTACGAAGCAGCTACTGGATGACA CCATTGGAATCCACCCCACCTGTGGTGAGGTATTTACAACCATGGAAATCACAAAGTCCTCAGGGCTGGA CATTACTCAGAAAGGCTGCTGAGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106609
Insert Size	1848 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106609.2, NP_001100079.2
RefSeq Size	2799 bp
RefSeq ORF	1848 bp
Locus ID	297437
Gene Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. There is evidence for two isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) contains two in-frame translation initiation codons: an upstream non-AUG (CUG) and a downstream AUG codon. The isoform (2) represented in this RefSeq results from the use of the AUG start codon. It has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript and the encoded protein are supported by orthologous mouse data.

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