Mapk4 (NM_019319) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216951 representing NM_019319 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAAAGGTGACTGCATCGCCAGTGTCTACGGGTACGACCTTGGTGGGCGCTTCACTGACTTCC AACCCCTCGGCTTCGGGGTCAACGGGCTGGTGCTGTCGGCTACGGACAGCAGGGCTTGCCGGAAGGTAGC CGTGAAGAAGATTGTGCTGAGTGACGCACGAAGCATGAAGCACGCGCTCCGAGAGATCAAAATCATCCGG CGCCTGGACCACGACAACATCGTCAAAGTGTACGAGGTACTGGGACCCAAGGGTAGCGATCTGCAGGGCG AGCTCTTTAAGTTCAGCGTGGCTTATATTGTCCAAGAGTACATGGAGACTGACCTGGCATGCCTGCTAGA GCAGGGCACGCTGACCGAGGAGCACGCTAAGCTATTCATGTACCAGCTGCTGCGTGGGCTCAAGTACATC CACTCTGCCAACGTGTTGCACAGGGACCTGAAGCCCGCCAACATTTTCATCAGCACGGAGGACCTCGTGC TGAAGATCGGGGATTTCGGGCTGGCCAGAATCGCGGACCAGCATTACTCCCACAAGGGTTATCTGTCGGA AGGGTTGGTGACAAAGTGGTACCGCTCTCCACGACTGCTCCTGTCCCCGAACAACTACACGAAAGCCATC GATATGTGGGCAGCTGGCTGCATCCTAGCGGAGATGCTCACGGGGAAAATGCTCTTTGCTGGGGCTCACG AGCTTGAGCAGATGCAGCTCATCCTAGACACCATCCCTGTAGTGCGGGAGGAAGACAAGGAGGAGCTGCT CAGGGTGATGCCGTCCTTTGTCAGCAGCACTTGGGAGGTGAAGAGGCCACTGCGTAAGCTACTCCCGGAT GTCAACCGTGAAGCCATTGACTTTCTGGAGAAGATCCTGACCTTTAACCCCATGGACCGCCTAACAGCTG AGATGGGGCTACAGCATCCTTACATGAGCCCGTATTCGTGCCCCGAGGATGAGCCCACCTCACAACACCC CTTCCGCATTGAAGACGAGATCGACGACCTCGTACTGATGGCTGCCAGTCAGAGCCAGCTCTCCAACTGG GACAGGTATCCCGTGAGCCTGTCGTCAGATCTGGAATGGCGGCCTGACCGCTGTCAGGACGCGAGCGAGG TGCAGCGCGACCCACGTGCTGGCTCTACTCCGCTGGCGGAGGATGTTCAGGTGGACCCACGCAAGGACTC ACAGAGCAGCTCCGAGCGCTTCCTGGAGCAGTCGCACTCGTCCATGGAGCGCGCCTTTGAGGCCGACTAT GGACGGTCCTGCGACTACAAAGTGGGGTCTCCGTCCTACCTGGACAAGCTGCTGTGGCGCGACAACAAGC CCCACCACTACTCGGAACCCAAGCTCATCCTGGACCTGTCGCACTGGAAGCAGGCGGCCAGCGCACCCCC CAGGGCTGCAGTAGCAGCAGACCCAGTGTCACGTGAGGATGAGCCAGCAAGCCTCTTTCTGGAGATCGCG CAGTGGGTCAAGAGCACACAAGGTGGCCCTGAGCGTGCCAGTCCTCCCCCTGATGCCCCAGAGCCCCGTC TCTCTGCTTCACCCCCGGGACACCCAACACCCATTGATGGGGGTGCCAGTCCCCAATTCGACTTAGATGT GTTCATCTCGCGTGCCCTAAAGCTCTGCACCAAACCTGAGGACCTACCGGAAAACAAACTGGGCGACCTG AATGGTGCGTGCATCTCCGAGCACCCCGGCGACCTCGTGCAAACAGAGGCCTTCTCCAAAGAAAGGTGGT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019319
Insert Size	1752 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019319.1, NP_062192.1
RefSeq Size	3596 bp
RefSeq ORF	1752 bp
Locus ID	54268
Gene Summary	mitogen activated protein kinase found in brain; may be involved in manic-depressive illness [RGD, Feb 2006]

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