Txndc2 (NM_001146004) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216895 representing NM_001146004 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAAGAAGAACCAAAAGCTGAGTAAGGACAAGGGGTTGGAGGTGAACAGCGTCCAAGCAGGAGCCC CTGAAGAGTCAGATGTGAAACTTAACAATGGAGGAAAAGCAAATGAAAGGGGTAGCAATGAGTTCTTGGA TACAGCCCAGTCCAAAGAAAAAGTCATTGCTTCCGTGGTCGGCAACATGCTTCATATGTCCACAGAAGAG TCTGAGCCCCCACAGCAGGTTAGTAGCACCTCCATGTTCTCAGAAAACACCATCTATCCCAAACACGAAG GGAGCCCCAAGTCCTCAACAAAAAACACTCAGCTGAAACAGGAAGACATTTCTAAAACCTCAAGCTACTC CAAGCAGACCAACTCTAGCAACATCCCCAAGTCCTTGGCAATAACCACCTATCCCAAACAGGGGAGCACT CTCAAGCCTGCAGCAAATGGTACCCATGACAGGGAGGCTGAAAAGCCCAAGTCCTCAGAAGACCTCATCC AATCCAAGAAAGGGGACATATTCAAGCCTTCAGAAGACTCCATCCAATCCAAGAAAGGGGACATGCCCAA GTCCTCAGAAGACCCCATCCAATCCAAAAAGGATGACACGGCCAAGTCTTTAGAAGATACCATCCAATCC AAGAATGGGGACATGCCCAAGTCCTCAGAAGACCCCATCCAATCCAAAAAGGATGACACGGCCAGGTCTT TAGAAGATTCCATCCAATCCAAGAAAGGGGACATGCCCAAGTCCTCAGACACCATTCAATCCAAGGAAAG CGAAACACCCAAGTTCTTGCAGGACACCATCCAATCCAAGGGAGGTAAAATCAACAAACAAGTGAAAGAC AGCATGAAGTCCAAGGAGAGTAAAATTCGCAAGCCCTTGAAAGACAGCATCCAGTCCAAGGAGAACAAAA TCCCCAAGTCCTCACAGGACTCCGCCCAGCCTAAGGAGGGTAAAATCCACAAGCCCTTAAAAGACAGCCT CCCATCCAAGGAGGGAGATATCTCTAAGCCCTCAGAAGACACCATCCAGGCCAAGGAAGAAATCACCGTG TCCCCAGAAGACACCATCCAGGCCAAGGAAGAAATCACCATGTCCCCAGAAGACACCATCCAGGCCAAGG AAGAAATCACCGTGTCCCCAGAAGACACCATCCAGGCCAAGGAAGAAATCACCGTGTCCCCAGAAGACAC CATGCAGTCTAAGGAAGAAATCACCGTGTCCCCAGAAGACACAGTCCAGTCCCAGGAAGGTGACATCAAG TCTTCAGAAGACGTGCAGCCCTCGGAGAACGAAATCTTCCCTTTTGAAGCAGAAATAGAGACCCTGGAGG AAGGCATGGTGAGAGTGATCAAAGACAAAGAAGAGTTTGAGGAGGTGCTCAAAGATGCTGGAGAGAAGCT GGTAGCTGTAGATTTCTCAGCCCCTTGGTGTGGCCCTTGCAGAAAGATGAGGCCACACTTCCATTCCCTG TCTTTGAAGCATGAAGATGTGATATTCTTGGAGGTGGATACTGAGGACTGTGAACAGCTGGTGCAAGACT GTGAGGTCTTCCACCTCCCGACTTTCCAGTTTTATAAAAACGAAGAAAAGGTGGGTGAATTTTCGGGTGC CCTTGTGGAAAAACTTGAAAAGAGCATTGCAGAATTAAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001146004
Insert Size	1653 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001146004.1, NP_001139476.1
RefSeq Size	1807 bp
RefSeq ORF	1653 bp
Locus ID	316777
UniProt ID	Q5XHX6
Gene Summary	Probably plays a regulatory role in sperm development. May participate in regulation of fibrous sheath (FS) assembly by supporting the formation of disulfide bonds during sperm tail morphogenesis. May also be required to rectify incorrect disulfide pairing and generate suitable pairs between the FS constituents. Can reduce disulfide bonds in vitro in the presence of NADP and thioredoxin reductase (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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