Adcyap1r1 (NM_001270581) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	PAC1-R; PACAP-R1; PACAP-R1A; PACAPR1; PACAPR1A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216738 representing NM_001270581 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGAGTCCTGCAGCTCTCCCTGACTGCTCTCCTGCTGCCTGTGGCTATTGCTATGCACTCTGACT GCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAGGATCCAGAGGGCCAACGACCTGATGGGACTAAA CGAGTCTTCCCCAGGTTGCCCTGGCATGTGGGACAATATCACATGTTGGAAGCCAGCTCAAGTAGGTGAG ATGGTCCTTGTAAGCTGCCCTGAGGTCTTCCGGATCTTCAACCCGGACCAAGTCTGGATGACAGAAACCA TAGGAGATTCTGGTTTTGCCGATAGTAATTCCTTGGAGATCACAGACATGGGGGTCGTGGGCCGGAACTG CACAGAGGACGGCTGGTCGGAGCCCTTCCCCCACTACTTCGATGCTTGTGGGTTTGATGATTATGAGCCT GAGTCTGGAGATCAGGATTATTACTACCTGTCGGTGAAGGCTCTCTACACAGTCGGCTACAGCACTTCCC TCGCCACCCTCACTACTGCCATGGTCATCTTGTGCCGCTTCCGGAAGCTGCATTGCACTCGCAACTTCAT CCACATGAACCTGTTTGTATCCTTCATGCTGAGGGCTATCTCCGTCTTCATCAAGGACTGGATCTTGTAC GCCGAGCAGGACAGCAGTCACTGCTTCGTTTCCACCGTGGAGTGCAAAGCTGTCATGGTTTTCTTCCACT ACTGCGTGGTGTCCAACTACTTCTGGCTGTTCATTGAAGGCCTGTACCTCTTTACACTGCTGGTGGAGAC CTTCTTCCCTGAGAGGAGATATTTCTACTGGTACACCATCATCGGCTGGGGGACACCTACTGTGTGTGTA ACAGTGTGGGCTGTGCTGAGGCTCTATTTTGATGATGCAGGATGCTGGGATATGAATGACAGCACAGCTC TGTGGTGGGTGATCAAAGGCCCCGTGGTTGGCTCTATAATGGTTAACTTTGTGCTTTTCATCGGCATCAT CATCATCCTTGTACAGAAGCTGCAGTCCCCAGACATGGGAGGCAACGAGTCCAGCATCTACTTACGGCTG GCCCGCTCCACCCTACTGCTCATCCCACTCTTCGGAATCCACTACACAGTATTCGCCTTCTCTCCAGAGA ACGTCAGCAAGAGGGAAAGACTTGTGTTTGAGCTTGGGCTGGGCTCCTTCCAGGGCTTTGTGGTGGCTGT ACTCTACTGCTTCTTGAATGGGGAGGTACAGGCAGAGATTAAGAGGAAATGGAGGAGCTGGAAGGTGAAC CGTTACTTCACTATGGACTTCAAGCACCGGCACCCGTCCCTGGCCAGCAGTGGAGTAAATGGGGGAACCC AGCTGTCCATCCTGAGCAAGAGCAGCTCCCAGCTCCGCATGTCCAGCCTCCCGGCCGACAACTTGGCCAC CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270581
Insert Size	1404 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270581.1, NP_001257510.1
RefSeq Size	6263 bp
RefSeq ORF	1404 bp
Locus ID	24167
UniProt ID	P32215
Gene Summary	regulates neural precursor proliferation; mediates inhibitory signaling for Shh-induced cerebellar granule precursor cell proliferation [RGD, Feb 2006] Transcript Variant: This variant (4) lacks two alternate in-frame exons and differs in the 5' UTR compared to variant (1). The resulting isoform (d) has the same N- and C-termini but is shorter compared to isoform a. Variants 4 and 5 both encode the same isoform (d). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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