Selenov (NM_001166396) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1562943; Selv
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216369 representing NM_001166396 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAACAAGGCGCGGAACCCCGCTCCCTCTTCAGTCCGGGCAAACACCCCGTCCCGGACACCCACTC CCGTCCGAACCGCGACCCCAGTCAGGGCCTCGACCCCGGCGCACAACCGGTCTCCGGTCCGAACATCCAT CCGGGTCCGGACTCCTGCTAACCCTGTTCCAATTCGGTTCCCTACTCCAGCCCCAGCCCCTGCCCCAACT CCAACCCCAGCCCCAACTCCAGCCCCAGTCCCGGCCGCAGCACCGGTGCGGACTCCAGCACCAGTCCGGG CCTCCATTCAAGGTCGGAGCTTTCCCACGATCTCTCCAGTGAGATTCTTACGGAACTTGGCCTTGCCCGC GGCACAGCCCCTTTCTTCCGGGGGTGCTGGCTCTCTCTCAAAGGATCTCACGCTCGCTCAAAAGCAGAAG CCCTCGATCCACAGCCTAGCGGAGGCCATCCAGGGGCCCTTTCCAGTGCTCACTCCCTCGGCCTCCTCGG AGACCCACGGCTCCATACCGGACCCCGCCCCTCCCACTGACTCCTTGGCTTCCACTGCCATGGCCTCCAG TACCTTGGACCCCATTCCGGGCCCCAAACCTACACTAGAGTTCTTGGCCTCCCCGCTGAAGGAAACCCCT GATTTGGGTAAGCTCTCCACAATTTCCCCAGCACAGAACTTCGTTTCCACTAAGGAGGTCCCCTCCACCA GCGAGGACGTCCCAACGGCCAACAGAATCTTGATCCGTGTGATGTACTGTGGCCTCTGAAGCTATGGCCT TCGGTACATTCTACTGAAAAAGACCCTGGAGCATCAATTTCCAAACCTTCTGGAGTTTGAGGAGGAAAGA GCTACCCAGGTTACAGGGGAGTTTGAAGTGTTTGTGGATGGGAAATTGATCCATTCCAAGAAGAAAGGCG ATGGCTTTGTGGATGAGACTAGTCTGAAGAAACTTGTGGGTGCTATTGATGAAGAGATCAAGAAAAGGTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001166396
Insert Size	981 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001166396.1, NP_001159868.1
RefSeq Size	1228 bp
RefSeq ORF	981 bp
Locus ID	499113
Gene Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is specifically expressed in the testis. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (1) represents the predominant transcript, and encodes a selenoprotein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript and the encoded protein are supported by orthologous mouse data. ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

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