Snrpc (NM_001271040) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Snrp1c; U1-C; U1C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215930 representing NM_001271040 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAGTTTTATTGTGACTACTGTGATACGTATCTTACCCACGATTCTCCGTCTGTGAGGAAGACAC ACTGCAGTGGTCGGAAACACAAAGAGAATGTGAAAGACTATTACCAGAAATGGATGGAAGAACAGGCCCA GAGCCTGATTGACAAAACAACGGCTGCATTTCAACAAGGAAAGATCCCTCCAGCTCCGTTCTCTGCTCCT CCGCCTGCAGGGGCCATGATCCCGCCTCCCCCCAGTCTCCCGGGTCCTCCTCGGCCTGGCATGATGCCTG CACCCCACATGGGAGGCCCTCCCATGATGCCAATGATGGGCCCCCCTCCTCCTGGGATGATGCCTGTGGG ACCAGCTCCTGGGATGAGACCACCCATGGGAGGCCATATGCCCATGATGCCCGGGCCTCCAATGATGAGA CCTCCCGCCCGCCCTATGATGGTGCCCACACGGCCTGGCATGACCCGGCCAGACAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271040
Insert Size	480 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271040.1, NP_001257969.1
RefSeq Size	1072 bp
RefSeq ORF	480 bp
Locus ID	361808
UniProt ID	D3ZCL3
Gene Summary	Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.[UniProtKB/Swiss-Prot Function]

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