Ctu1 (NM_001106251) Rat Untagged Clone
CAT＃: RN214171
Ctu1 (untagged ORF) - Rat ATP binding domain 3 (Atpbd3), (10 ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Atpbd3; RGD1308740 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214171 representing NM_001106251
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCCCGACGTGCTTCTCCTGTCACAAGGCCCGTGCTGCCCTCCGCCGCCCACGCTCTGGGCAGG CTTTGTGTGGCCCCTGCTTCTGTGCTGCCTTTGAGGCTGAGGTGCTGCGTACGGTGCTTGCTGGGCACCT GCTGCCGCAGGGCGCTGTAGTGGCTGTGGGCGCCTCTGGCGGCAAGGACTCCACGGTGCTGGCACACGTG CTGCGTGAGCTTGCACCACGCTTGGGCATCACACTGCACCTGGTGGCCGTGGATGAGGGCATAGGCGGCT ACCGCGACGCTGCGCTGGAGGCGGTGCGCAGTCAGGCAGCGCGCTGGGAGCTGCCGCTCACCATCGTGGC CTATGAAGATCTCTTCGGAGGCTGGACAATGGACGCTGTGGCCCGCAGCACCGCAGGCTCCGGCCGCAGT CGCTCCTGCTGCACCTTCTGCGGGGTGCTGCGGAGGCGCGCGCTGGAGGAGGGTGCCCGCCTTGTGGGAG CTACTCACATTGTGACAGGTCACAATGCTGACGACATGGCTGAGACGGTGCTCATGAACTTCCTGCGTGG TGATGCTGGGAGGCTGGCGAGGGGTGGAGTGCTGGGTTCCACAGGCGAAGGATGCGCACTTCCCCGGTGC CGGCCTCTGCAGTTTGCCTCGCAGAAGGAGGTGGTGCTGTACGCACATTTCCGCCACCTCCGCTACTTCT CCGAGGAGTGTGTGTATGCCCCTGAGGCTTTCCGTGGCCATGCTCGGGACCTGCTTAAGCTCCTGGAGGC AGCCAGACCATCGGCCGTGCTGGACCTCGTGCACTCGGCAGAGCGCCTAGCTCTGGCTCCGGCAGCAAAG CCCCCTCCTCCAGGCACCTGCTCCCGCTGCGGAGCGCTGGCCAGCAACAAGCTCTGCCAGGCCTGTGCAC TCCTGGATGGCCTCAACCGAGGCCTGCCGCGCCTGGCCATCGGCAAGGGCCGCCGTGTGTTGCAAGTTGA GCCGCCACCTCTAGGGAACCCAAGCCGGGTCACCAGTGACCCTGTGGCCCTACCAGAACCCTGCACCTGC AAGCAATCCAAAGATGAATCCAACCCATGCGGAAATGGAGGGGACCGGGCAGGAGCCACCTGTGTATCGA AGAGTGACCTGAGCCCCGTGGCGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001106251 |
| Insert Size | 1149 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001106251.1, NP_001099721.1 |
| RefSeq Size | 2245 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 292847 |
| UniProt ID | B1WBV0 |
| Gene Summary | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214171 | Ctu1 (Myc-DDK-tagged ORF) - Rat ATP binding domain 3 (Atpbd3), (10 ug) |
CNY 3990.00 |
|
| RR214171L3 | Lenti ORF clone of Ctu1 (Myc-DDK-tagged ORF) - Rat ATP binding domain 3 (Atpbd3), (10 ug) |
CNY 6080.00 |
|
| RR214171L4 | Lenti ORF clone of Ctu1 (mGFP-tagged ORF) - Rat ATP binding domain 3 (Atpbd3), (10 ug) |
CNY 6650.00 |
