Arhgef28 (NM_001108542) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rgnef
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214009 representing NM_001108542 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGAGCTGCAGTGAAGTGCCTCTTTACGGACAGAGGACGGTCTATGCAAAGTTCGGCAAAAACG TTTATCTTCCTGAAGATGCGGAGTATTACTTTATTTATGATGGGTCGCATCAGAGGCACGTGGTGATTGC GGATCGTGTTGAGGATAATGTTCTCCAGTCTATCATTCCAGGCCATTGGCTTCAAGAGACGGTGATGGTA TCTGTGTGCCTCTGCTCAGAAGGGTATTCCCCGGTGACCATGGGCTCTGGCTCAGTGACCTATGTGGACA ACATGGCCTGCAGGCTGGCTCGTCTGCTGGTCACTCAGGCTGACCGACTCACGCCCTGTAGTCACCAGAC ACTGCTGACCCCGTTTGCCCTGACAGCAGAAGCGCTGCCTGCCTTGGATGAGGAACTCGTGCTGGCTCTG ACCCAACTGGAGCTGCCTTTGGGTTGGACAGTCCTGGGAAATTCCTCACTTGAAGTGTGTCTACACAGAG AGTCTCTCCTGCACCTGGCAGTGAGGTGGGCCCTGCCTAAGCTATTCCATTTCCTCTTGTGCCTCCCTGG AGGAGTCAAGGCCTTGGAGCTACCTAATGAAGAGGCCACCACACCATTAGACTTAGCCTTGCATGGCGGA CACTCTATGCTGGTGGAAGACATCTCAAATTTTCAGGGTCGCCGCTCCCCAGGCTTCTCCCGACTGCGTC TTAATGAAGATGCCACGCTGCAGTTCGATCGGTCATCAGAAACGTTGACGCTGACCGTTAACCACACAGC TGAGCATCTGTTGGAAGCAGATATCAAACTCTTCCGGAAGTATTTCTGGGACAGAGCCTTTCTTGTCAAG GCTCTTGATCAAGAAGCCAAGACAGAGAGAGCAACCATGCCTTCTGGAGCTGCGGAAACTGAAGAAGAGG TTAGGAATCTGGAGTCTGGCAGATCACCCTCAGAAGAGGAGGAGGATGGACAGCTCGTCAAAAGCCAGGC CGACGGGCCGAGTGAACAGGAAGACCAGGACCGCCTAGTTTTGGATCACTCCTTTGATGGCCTCAAAAAG TCCAAGCACGCCCCAGCGTCCCTTGCTGCAGGCCAGCTCTCTGACGTGCCGAATGGGGGTGACGAAGTCT ACGCTAACTGTATGGTGATTGATCAGGTTGGCGATTTGGACATCAACTATATAAACATAGAGGGACTCTC CACCCATACTAGCCCGGAGTCCATGAGGTCCACTCTGGGGCCTCAGGCTTGTAAGCACATCCTTCCTCCT GACACCAGTCCCTGCGGGCACCTGCTCTGTGAGGACAGTGACAGGACCCTGGATGCTGCAGCCTCTCAGT CCTGCATGTCACCACCCAGTTCTCACACTTCCAACTTGAACCTTTCTTTTGGTCTCCATGGATTTGTAAA GGAGCAGAGTCATCTCAAGAAAAGAAGTTCTAGCCTGGATGCCCTCGTTGCCGACAGTGAAGAGGAAGGG CGTTCTGAGCCCCCCATCTGCTATGCTGTCGGCTCTCAGAGTTCCCCCAGAACTGGGCTCCCCGGCGGAG ATGAACTGGATTCTTTTGATGCGAACACCGAACCAGACTGTAACATCTCTAGGACCGAATCACTTTCCCT GTCCAGCACTCTGCATTCGAAGGAGTCCCTGCTTTCTGGGATCCGATCACGCTCCTACTCCTGCTCATCA CCCAAAATCTCTTCAGGAAAATCCCGGCTAGTGCGCGATTTCACAGTGTGCAGTTCCAGTGAAGAACAGA GATCATATAGTTTCCAGGAGCCACCGGGAGAAAAAAGAATTCAGGAAGAAGAATGGGACGACTACATCAT TCCTGCCAAAGCAGAGTCCGAAAAGCACAAAGTGAGCCGGACTTTCAGCTTCCTCATGAACAGGATGACC AGTCCTCGGAACAAATCTAAGATGAAAAACAAGGACACTAAAGATAAAGAAAAACTCAATCGACATCAGT TTGTCCCGGGAACGTTCTCTGGAGTTCTGCAGTGTTCCGGTTGTGACAAAACACTCCTGGGGAAAGAGTC ACTCCAGTGTGCCAACTGCAAGGCCAACACACACAAGGGCTGCAAAGACACTGTGCCTCCGTGTACCAAG AAATTCCAAGACAAATACAACAAGAACAAACCACAGACCATCCTCGGAAGCTCTTCAGTTAGAGACGTCC CACCGCCTGGGCTCTCCTTGCACCCTTCCCCCTCCATGCCTATTGGACTGCCAGCTGGAAGGAAGGAAAC TGCAGGGCAGGCACATCCGTTGTCCAGAAGTGTCCCAGGCACCACTTTGGAAAGCTTCAGGAGGGCAGTG ACATCCTTGGAGTCTGAGGGTGACAGTAACAGCTGGAGAAGCAGGTCTCATTCCGATGAGCTGTTCCAGT CCATGGGCTCGTCTCCTTCCACGGAGTCATTCATGATGGAAGATGTCGTGGATTCTTCTCTGTGGAGTGA GCTCAGCAGTGATGCTCAAGAGTTTGAAGCTGAGTCCTGGAGTCTCGTGGTGGACCCTTCCTTCTGCAGC AGACAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTGATGCAGACGGAGGTGCACCACA TCCAGACACTGCTCATCATGTCTGAAGTCTTCAGGAAGGGCATGAAGGAGGAGCTGCAGCTGGACCACAG CACGGTGGACAGGATCTTCCCCTGCTTAGATGAGCTGCTGGAGACTCACAAGCATTTCTTCTTCAGCATG AAGGAGCGCAGGCAGGAGTCCTGCACCGGCAATGACAGGAACTTTGTGATCAACCAAATCGGAGACATTT TGGTACAACAGTTTTCAGAAGAAAATGCAAGCAAGATGAAGAGGATCTATGGAGATTTCTGCAGCCATCA CAAAGAAGCCATGAGCCTCTTTAAAGAGCTCCAGCAGAACAAAAAGTTTCAGAATTTTATTAAGATCCGA AATAGCAACCTGTTGGCCCGTCGCCGTGGAATCCCAGAGTGCATCCTGCTTGTCACCCAGCGCATCACGA AGTACCCGGTCTTGGTGGAGAGGATCCTGCAGTACACCAAGGAAAGGACCGAAGAGCACAGAGACCTGTG TAGAGCTCTTGGCTTAATTAAAGACATGATCGCAGCCGTGGACCTGAAAGTCAGTGAATATGAGAAGAAC CAAAAATGGCTCGAGATCCTAAATAAAATCGAGAACAAAACATACACAAAGCTCAAAAATGGCCACGTGT TTAGAAAGCAGGCGCTGATGAGCCAAGAAAGGGCTCTTCTGCACGACGGGCTCGTTTACTGGAAAACTGC GACAGGCCGTTTCAAAGACATCCTGGCCCTGCTTCTCACCGATGTGCTGCTCTTCTTGCAAGAAAAAGAC CAGAAGTATATCTTTGCAGCTGTTGACCAGAAGCCTTCAGTTATTTCCCTTCAGAAGCTCATCGCTAGGG AAGTCGCCAACGAGGAGAGAGGCATGTTTCTCATCAGTGCTTCGTCAGCGGGTCCCGAGATGTATGAAAT CCACACCAGTTCCAAAGAGGAACGGAATAACTGGATGAGGCGGATCCAGCAGGCAGTAGAAAGTTGTCCA GAAGAAGAAGGAGGAAGGACAAGTGAGTCTGATGAAGAGAGGCGGAAAGCTGACGCTAGAGTGGCCAAAA TTCAGCAATGTCAAGAGATTCTCAGCAACCAAGACCAGCAGATCTGCACATATTTGGAAGAAAAGCTGCA CATCTACGCTGAGCTTGGGGAACTAAGTGGCTTTGAGGATGTCCACCTAGAACCCCATCTGCTCATCAAG CCCGACCCCGGAGAACCTCCCCAGGCAGCCTCCCTGCTTGCAGCAGCCCTGAGAGAAGCTGAGAGTCTGC AAGTTGCTGTGAAGGCTTCAAAGCTGGCTGATGTAAGCCAGCCATCTGAAGAAGGCCCTGGAGGGGCTGT CCTGGCTGACACACTCAGTGCACAAGATGCACCAGCATCGCCGACTGCCTTCACAGAAGGGACAGAAGGA AGAGGCTGTTGGGATGTGGATCCCCGGCTCCAGGGTGTGGTAACGGACTTGGCGGTCTCTGATGCAGGGG AGAAGGTGGAATATAGAAGTTTTTCCGGTTCTTCACAATCAGAGATTATACAAGCGATACAGAACTTAAC GCGTCTCCTCTACAGCCTTCAGGCTGCCTTGACCATCCAGGACAGCCACATTGAGATCCACAAGCTAGTT CTACAGCAGCAGGAGAGCTTGGCCCCTAGCCACTCCTTCCGAGGTGGCCCCTTGCAAGACCAGGAGAAAT CCCGTTACCTGGAAAAGCATCGTGAAGAGCTGGCCAACATCCACAAGCTCCAGTACCAGTTCCAACAAGA GCAGCGGCGTTGGCACCGCACATGTGACCAGCAACAGCGTGAGCAGGAGGCCCAAGAGAGCTGGCTGCAG GCACGGGAACGTGAGTGCCAGTCGCAGGAGGAATTACTGCTGCGCCACCGTAGCGAACTGGACCACCAAC TCCAGGAGTACCAGCAGAACCTCGAGCGGTTGCGGGAGGGCCAGAGGATGGTGGAGAGGGAGAGGCAGAG GATGCGTGACCAGCAGGGTCTGCTGGGCCACTGTAAGCATAGTCGCCAGAGGAGCCTCCCAGCCGTCTTC TCTCCGGGGAGCAAAGAGGTAACGGAACTTAATCGAGCTGAGAGTTTGTGTCATGAAAAATCATTTTTCC TCAATGACGCCTTCACACACATGTCGCTTAACACATCCAATAAACCAAACCCATCAGGTGCCCCCTGGGA CGCTCACCCCCCAGGTGGGTCTCATCTTGATTTGGCAAGGACTAGTGATAGTCAGATAGAAGTGAAGATG GACGTTTCTCAGCCTTCCGATGTCAACTCTGAACTCTGGACAACAGGGCTGGGCCCTCAGAGACCTGCTC GCCAAGAAAGCATCAAGGATTCTTGTAAAAATGTTGCAGATTTGAACTCCTTCCAGACTGAATCCCCAGA TCCCCAGGACTCAAATCAACGTGGCCCTCAGCCACAGACACTCATAACAGAAGCAAAACTAACTCCCCCG ATGGCGGCCGGACATGGTGGAGATGCTGGGGATGGAGCTGAAGAGAACATTGTTTACCTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001108542
Insert Size	5103 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108542.1, NP_001102012.1
RefSeq Size	6195 bp
RefSeq ORF	5103 bp
Locus ID	361882
Gene Summary	Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.[UniProtKB/Swiss-Prot Function]

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