Atp10a (NM_001141935) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213951 representing NM_001141935 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGGAGTCCGCGGCGGCCGAAGAGCCCGCATCCCCGGGGCGGCGGCGTCCTCGACGAAGGCGCT GGGAGGGCCGGACGCGCACGGTGCGCTCCAACCTGCTGCCGTCCCTGGGCACCGAAGACTCCACTGCCGG AGCCTCCAAGGGCGAACGGCTGCTGATGCGCGGCTGCATCCAGCACCTGGCCGACAACCGCCTCAAGACC ACCAAGTACACTCTGCTGTCCTTCTTGCCCAAGAATCTCTTCGAACAGTTCCACCGCCTAGCCAATGTGT ACTTCGTCTTCATCGCGCTACTCAACTTTGTGCCGGCAGTGAACGCTTTCCAGCCGGTCCTGGCACTGGC GCCTGTGCTCTTTATTCTGGCGGTCACTGCCATCAAGGACCTGTGGGAGGACTACAGCCGCCACCGTTCG GACCACGAAATCAACCACCTGGGCTGCCTTGTCTTCAGCAGGGAAGAAAAGAAGTATGTGAATCGATACT GGAAAGAAATCCGAGTGGGCGACTTTGTCCGTCTTTGCTGCAATGAAATCATACCTGCGGACATTTTGCT GCTGTCATCCAGTGACCCTGATGGGTTGTGCCACATCGAGACTGCTAACCTGGATGGAGAGACCAACCTG AAGCGGCGGCAAGTGGTCCGTGGCTTCTCGGAGCTTGTCTCTGAATTCAATCCCTTAACGTTCACCAGCG TAATCGAATGTGAGAAGCCAAACAATGACCTGAGTAGGTTTCGTGGCTGCATAATCCACAGCAATGGGGA GAAGGCTGGGCTGCATAAGGAAAACTTGCTGCTTCGAGGGTGCACCATCAGAAACACAGAAGCAGTGGCT GGGATTGTCATCTATGCAGGACATGAAACCAAAGCCCTCCTGAACAATAGTGGGCCCCGCTACAAGCGCA GCCAGCTTGAAAGGCAGATGAACTGTGATGTCCTCTGGTGTGTCCTTCTCCTCGTTTGTATATCTCTATT TTCAGCAGTTGGACATGGATTGTGGGTTCGGCGCTATCAAGAGAAAAAAGCACTGTTTGATGTTCCCGAG TCTGATGGCAGCTCTTTATCCCCAGCCACTGCTGCAGTTTACTCGTTTTTGACAATGATAATTGTTCTGC AGGTTTTGATTCCAATTTCCTTATATGTTTCCATTGAAATTGTTAAAGTGTGTCAAGTATACTTCATTAA CCAAGATATAGAGCTGTACGATGAAGAGACAGATTCACAGCTGCAGTGCCGAGCTCTGAACATAACAGAA GACTTAGGGCAGATAAAATACATCTTCTCAGACAAAACTGGCACATTAACTGAGAACAAGATGGTCTTTC GAAGATGCACTGTGTCCGGCATAGAATATTCTCATGATGCTAATGCCCAGCGACTGGCCAAGTATCAAGA GGCGGACTCAGAAGAGGAGGAAATGGTGTCCAAGGTGGGCCCAATATCCCACCGTGGCAGCACTGGGAGC CATCAGAGCATCTGGATGACTCATAAGACCCAGAGTATCAAGTCCCACCGGCGTACAGGCAGTCGGGCTG AGGCCAAGAGAGCCAGCATGCTGTCTAAGCACACTGCCTTCAGCAGCCCCATGGAGAAAGACATTACCCC TGACCCCAAGCTGCTGGAGAAGGTGAGCGAGTGTGACCGGTTCCTCTCCATTGCAAGGCACCAGGAGCAC CCTCTGGCCCATCTTTCACCAGAGCTGTCTGATGTTTTTGATTTCTTCATCGCACTCACCATCTGCAATA CAGTAGTTGTCACCTCTCCAGACCAGCCACGACAAAAGGTAAGAGTGAGGTTTGAGCTGAAGTCCCCAGT GAAGACCATAGAAGATTTCCTTCGGAAGTTCACACCCAGCCGCCTGGCTTCTGGCTGCAGCAGTATCGGG AACCTCACCAACAATAAATCCAGCAACAAATCTGGATCAACTTTTCTGCCCAGCCTGTCTCAGGACAACA TGCTTCTCAGGCTGGAGGAGAGGCTAGGTCAAACAGCGCCAGTCATTGCCAGCAATGGCTATGCCAGCCA GGCAAGCCAGGCGGAAAGCTGGACCTCAGAGTGCACCTCAGAGCAGAAGTGTCCAGGGGACCAGAGGGAG CAGCAAGAGGGGGAGCTTCGATATGAGGCTGAGAGCCCAGATGAGGCTGCGCTGGTGTATGCAGCCAAAG CCTATAACTGTGCACTGGTGGACCGGCTTCACGACCAGGTGTCTGTGGAGTTGCCCCACCTAGGCAGGCT CACCTTTGAGCTGCTGCACACACTTGGCTTCGACTCCATCCGCAAGAGGATGTCAGTGGTGATCCGACAC CCACTGACTGACGAGATCAATGTGTACACCAAGGGGGCTGATTCAGTGGTCATGGACCTTCTGCTGCCCT GTTCCTCAGATGACGCCAGAGGAAGGCATCAGAAAAAGATCAGGAGTAAAACACAAAATTACCTCAACCT TTATGCAGTAGAAGGCCTGCGCACCCTGTGCATTGCCAAGAGGGTTCTGAGCAAAGAAGAATATGCCTGC TGGTTGCAGAGCCACATAGAAGCTGAGACATCTGTGGAGAGCCGTGAGGAGCTCCTCTTCCAGTCTGCTG TTCGCCTGGAGACGAACTTGCACCTGCTGGGTGCCACTGGGATAGAAGACCGCCTTCAGGATGGAGTACC TGAAACCATTGCCAAATTACGACAAGCTGGCCTGCAGATTTGGGTTCTCACAGGTGACAAACAAGAAACA GCCATTAACATTGCATATGCCTGCAAATTGCTGGACCATGGTGAAGAGGTCATCACACTGAATGCTGATT CTAGGGAAGCATGTGCAGCCCTGCTGGACCAATGCCTCTGTTATGTGCAATCCAGAAACTCTAGAAGCGT CCCTCAGAACTCTGAAAGCAGCTTCAGTGTGGGGTTCTCTTTCAACCCAGCCTCCACATCCACTGACTCG AGCCCCAGCCCAAGCCTTGTGATTGATGGGAGAAGTCTGGCCTATGCCCTTGAGAAAAGCCTGGAGGACA AATTTCTTTTCCTTGCCAAGCAGTGTCGCTCCGTCCTCTGCTGCCGATCGACGCCTTTGCAGAAAAGCAT GGTGGTGAAGCTTGTCAGAAACAAGCTCAAGGCCATGACCCTGGCCATAGGTGATGGGGCCAATGATGTC AGCATGATCCAGGTGGCAGATGTAGGCGTGGGGATCTCAGGCCAGGAAGGCATGCAGGCTGTGATGGCAA GTGACTTTGCAGTGCCAAGATTCCGATACCTGGAGAGACTTCTAATCGTTCATGGACACTGGTGCTACTC CAGACTGGCCAACATGGTGCTGTATTTCTTCTACAAAAATACAATGTTTGTGGGTCTCTTGTTTTGGTTC CAGTTTTACTGTGGCTTTTCTGCATCTGCCATGATTGATCAGTGGTATCTGATTTTCTTCAATCTGCTCT TCTCATCGCTTCCTCAGCTGGTAACTGGGGTGCTGGATAAAGATGTTCCAGCTGACATGTTGCTGAGAAA GCCACAGCTTTACAAGAGTGGCCAAAACATGGAGGAATACAGTCCACGAGCATTCTGGTTAAACATGGTG GATGCTGCCTTCCAAAGCCTGGTCTGCTTTTTCATTCCCTATCTGGCCTACTATGACTCCGATGTGGATG TATTTACCTGGGGGACCCCTGTCACAGCAATAGCATTGTTCACCTTCCTGCTGCACCTGGGTATTGAAAC CAAAACCTGGACCTGGCTCAACTGGTTAGCCTGTGGCTTCAGTACCTTTTTGTTTTTCTCCGTGGCTCTA ATTTACAACACTTCCTGTGCCACATGCTATCCTCCATCTAACCCTTACTGGACCATGCCGACATTACTGG GAGACCCTCTGTTCTACTTGACTTGTCTCATCGCACCTGTTGCTGCATTGCTGCCCAGATTGTTTTTCAA AGCCCTCCAGGGGAGCCTTTTCCCCACCCAACTCCAGCTAGGACGCCAGTTGGCCAAGAAATCCCTCAAC AAGTTCAGCGTTCCCAAAGAGACTTTTGCTCAGGGACAACCACCTGGACACACAGAACCTGAGCTATCAG AAAGGAAGGCCATGGGACCTTTTGAGTCTCTCCCTAGAGACTGTGCCTCTCAAGCTTCCCAGTTCACACA GCAGCTGGCCTGTTCCCCAGAGGCCAGTGGGGAGCCCAGTGCAGTGGATACAAACATGCCACTGAGAGAG AACACCTTGCTGGAAGGACTTGGCTCTCAGGCCTCAGGGTCTGCTATGCCCAGAGAGGCTATTTCAGAAG ACTGTCCTGGGGACTCCAAGAGGAAATCCACCAGTGCTAGTCAAACAGCCCCCTTGTCGTCACTCTTCCA TCTGCCCAGCTTTGGCTCACTAAACTGGATCTCCTCACTATCACTGGCCAGTGGTCTGGGAAGTGTCTTG CAATTGTCCAGAAGTAGTTTACAGATGGACAAGCAGGATGGTGAGTTCCTGTCCAACCCACCACAACCAG ATCAGGACCTGCACAACTTACAGGGACAAGTTACAGGCTACTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001141935
Insert Size	4527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001141935.1, NP_001135407.1
RefSeq Size	5472 bp
RefSeq ORF	4527 bp
Locus ID	365266

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