Myo7a (NM_153473) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213648 representing NM_153473 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTATTCTACAAAAGGGGGACTACGTGTGGATGGACCTGAAGTCGGGCCAGGAGTTCGATGTGCCCA TCGGGGCCATGGTAAAGCTCTGTGAGTCTGGGCAGATCCAGGTGGTGGATGATGAAGGCAATGAACACTG GATTTCCCCTCAAAATGCCACGCACATCAAGCCAATGCACCCCACATCGGTGCATGGTGTGGAGGACATG ATCCGCCTGGGGGATCTCAACGAGGCAGGCATTCTTCGAAACCTTCTCATTCGCTACCGGGACCATCTCA TCTACACATACACGGGTTCCATCCTGGTGGCCGTGAACCCCTACCAGCTGCTGTCCATCTACTCGTCAGA GCACATCCGCCAGTATACCAACAAGAAGATAGGGGAGATGCCCCCCCACATCTTTGCCATTGCCGACAAT TGCTACTTCAACATGAAACGCAACAACCGGGACCAGTGCTGTATCATCAGCGGGGAATCAGGAGCTGGCA AGACAGAGAGCACAAAGCTGATCCTGCAGTTCCTGGCGGCTATCAGTGGACAGCACTCATGGATCGAGCA GCAGGTGCTGGAGGCCACCCCGATCCTGGAAGCATTTGGGAATGCCAAGACCATCCGCAATGACAACTCC AGCCGCTTTGGCAAGTACATTGACATCCACTTTAACAAGCGTGGTGCCATTGAGGGCGCAAAAATAGAGC AGTACCTGCTGGAGAAGTCACGAGTCTGCCGCCAGGCCCCTGATGAGAGGAACTATCACGTGTTCTACTG TATGCTGGAGGGCATGAATGAGGAGGAGAAGAAGAAGCTAGGCCTAGGCCAGGCCGCTGACTACAACTAT TTGGCAATGGGTAACTGCATCACCTGTGAGGGCCGTGTGGACAGTCAGGAGTATGCCAACATCCGCTCAG CCATGAAGGTGCTCATGTTCACAGACACGGAGAACTGGGAGATCTTGAAGCTTCTGGCCGCCATCCTACA CATGGGCAATCTGCAGTACGAGGCCCGTACGTTTGAGAACTTGGATGCGTGCGAAGTCCTCTTCTCCCCA TCGCTGGCCACGGCAGCTTCTCATCTCGAGGTGAATCCCCCAGACCTGATGAGCTGCCTCACCAGCCGCA CCCTCATCACCCGTGGGGAAACGGTCTCCACCCCTCTCAGCAGGGAGCAGGCGCTGGATGTGCGAGATGC CTTTGTCAAGGGCATCTATGGGCGGCTCTTTGTATGGATTGTGGAGAAGATCAATGCAGCAATCTACAAA CCACCCTCTCAGGAAGTGACGAACTCTCGCCGGTCCATCGGTCTCCTGGACATTTTTGGGTTTGAGAACT TCACCGTGAACAGCTTTGAGCAGCTCTGCATTAACTTTGCCAATGAGCACCTGCAGCAGTTTTTCGTGCG ACACGTGTTCAAGTTGGAGCAGGAGGAGTATGACTTGGAGAGCATTGACTGGCTGCACATCGAGTTCACT GACAACCAGGAGGCCCTGGACATGATCGCCAACCGGCCTATGAACGTCATCTCCCTCATTGACGAAGAGA GCAAGTTCCCCAAGGGCACGGATGCCACCATGCTGCATAAGTTGAACTCACAGCACAGGCTCAATGCCAA CTACGTCCCACCCAAGAACAGCCATGAGACCCAGTTTGGAATCAACCACTTTGCGGGCATTGTCTACTAT GAGAGTCAAGGCTTCCTGGAGAAGAACAGAGACACCCTGCATGGGGACATCATCCAGCTGGTCCACTCTT CCCGGAACAAGTTTGTAAAGCAGATTTTCCAGGCCGACGTTGCTATGGGTGCAGAGACCAGGAAGCGCTC GCCTACACTCAGCAGCCAGTTCAAGCGGTCTCTGGAGCTGCTGATGCGCACACTGGGCGCCTGCCAGCCC TTCTTTGTACGATGCATCAAACCCAATGAGTTCAAGAAGCCCATGCTCTTCGACCGGCACCTGTGTGTAC GCCAGCTGCGCTATTCGGGCATGATGGAGACTATCCGCATCCGCCACGCAGGCTACCCCATTCGCTACAG CTTTGTGGAGTTTGTGGAGCGCTACCGCGTGCTGCTGCCTGGGGTGAAACCAGCATACAAGCAGGATGAC CTCCAAGGGACATGCCAGCGCATGGCTGAGGCCGTGCTGGGCACTCACGATGACTGGCAGATTGGCAAAA CCAAGATCTTTCTGAAGGACCACCATGACATGTTGCTGGAGGTGGAGCGGGACAAGGCCATCACAGACAG AGTCATTCTCCTCCAGAAGGTCATCCGGGGCTTCAAAGACAGGTCCAACTTCCTGAGATTGAAGAGTGCA GCCACACTGATCCAGAGGCATTGGCGGGGCCACCACTGTAGGAAAAACTATGAGCTGATTCGTCTTGGCT TCCTGCGGTTGCAGGCCCTGCACCGCTCCCGGAAACTGCACAAACAGTACCGCCTGGCCAGACAGCGCAT AATAAAGTTCCAGGCCCGCTGCCGGGCCTACCTGGTGCGCAGGGCCTTCCGCCACCGCCTCTGGGCCGTG ATCACCGTGCAGGCCTATGCCCGGGGCATGATTGCGCGCCGGCTACACCGGCGCCTCCGAGTTGAGTACT GGCGGCGCCTTGAGGCAGAGAGGATGCGGCTGGCAGAGGAGGAGAAGCTAAGAAAGGAGATGAGTGCCAA GAAGGCCAAGGAGGAGGCGGAGCGCAAGCATCAGGAGCGCCTGGCCCAGCTAGCCCGCGAGGATGCAGAG CGGGAACTGAAGGAGAAGGAAGAGGCTCGGCGGAAGAAGGAGCTGTTACAGCAGATGGAGAGGGCCCGCC ATGAGCCTATCAACCACTCAGATATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAAGTGGCCTGCC AGGCCAGGAGGGCCAGGCACCTAGTGGCTTTGAGGACCTGGAGCGAGGGCGGAGGGAGATGGTGGAAGAG GATGTCGATGCTGCCCTGCCCCTGCCTGACGAGGATGAGGAGGACCTTTCTGAGTACAAATTCGCCAAAT TTGCTGCCACCTACTTCCAGGGCACAACCACACACTCCTACACCCGGAGGCCCCTCAAGCAGCCACTGCT CTACCACGATGATGAGGGTGACCAGCTGGCGGCACTGGCTGTCTGGATCACCATCCTCCGGTTCATGGGG GACCTCCCAGAGCCCAAGTACCACACAGCCATGAGCGATGGCAGTGAGAAGATCCCTGTGATGACTAAGA TCTATGAGACCCTGGGCAAGAAGACATACAAGAGGGAGCTGCAGGCCCTGCAGGGCGAGGGCGAGGCCCA GCTCTCTGAGGGGCAGAAGAAGACCAGTGTGAAACACAAGTTGGTACACTTGACACTGAAGAAGAAGTCC AAACTCACAGAAGAGGTGACCAAGAGGCTGCATGATGGGGAGTCCATGGTACAGGGCAACAGCATGCTGG AGGACCGGCCCACCTCAAATCTAGAGAAGCTGCACTTCATCATTGGCAACGGTATCCTGCGACCTGCACT GCGGGATGAGATTTACTGCCAGATCAGCAAGCAACTCACACACAACCCATCCAAGAGCAGCTATGCCAGG GGCTGGATCCTTGTGTCTCTGTGTGTGGGATGCTTCGCCCCTTCTGAGAAGTTTGTTAAGTACCTACGGA ACTTCATCCATGGAGGCCCACCCGGCTATGCCCCCTACTGTGAGGAGCGCCTGAGAAGGACCTTTGTCAA TGGAACTCGGACACAGCCACCCAGTTGGCTGGAACTGCAGGCCACCAAGTCCAAGAAGCCCATCATGTTG CCCGTGACCTTCATGGACGGGACCACTAAGACCCTGCTAGCAGATTCAGCAACCACAGCCAAGGAACTAT GCAATGCTCTGGCTGACAAGATCTCACTCAAGGACCGCTTTGGCTTCTCCCTCTACATTGCTCTGTTCGA TAAGGTATCCTCCCTGGGCAGTGGCAGTGACCACGTCATGGACGCAATCTCCCAGTGCGAGCAGTATGCC AAGGAGCAGGGTGCACAGGAGCGCAACGCCCCCTGGAGGCTCTTCTTCAGAAAGGAGGTCTTCACACCCT GGCACAACCCCTCGGAGGACAACGTGGCCACCAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTT TGGGGAGTACAGGTGTGAGAAGGAGGATGACCTGGCCGAGTTGGCCTCCCAGCAGTACTTTGTGGACTAT GGTTCTGAGATGATTCTGGAGCGTCTGCTGAGCCTCGTGCCCACTTACATTCCTGACCGTGAGATCACAC CGCTAAAGAATCTTGAGAAGTGGGCACAGCTGGCTATTGCTGCCCACAAGAAGGGAATTTATGCCCAGAG GAGAACTGATGCCCAGAAGGTCAAACAGGATGTGGTCAATTATGCCCGTTTCAAGTGGCCCTTGCTCTTT TCCAGGTTTTATGAAGCCTACAAATTCTCAGGCCCTCCCCTCCCTAAGAGTGACGTCATCGTGGCTGTGA ACTGGACGGGCGTCTACTTCGTGGACGAGCAGGAGCAGGTGCTTCTGGAGCTGTCCTTTCCGGAGATCAT GGCTGTGTCCAGCAGTAGGGGAGCAAAACTGATGGCCCCCAGCTTTACACTGGCCACCATCAAAGGGGAT GAGTACACCTTCACATCTAGCAATGCTGAGGACATCCGTGACCTGGTGGTCACCTTTCTGGAGGGGCTCC GGAAGAGGTCTAAGTATGTGGTGGCACTGCAGGACAATCCCAACCCTGCTGGTGAGGAGTCGGGCTTCCT CAGCTTTGCCAAGGGAGACCTCATCATTCTCGACCATGATACCGGCGAGCAGGTCATGAATTCAGGCTGG GCCAATGGCATCAACGAGAGAACCAAGCAGCGTGGAGACTTCCCCACCGACTGTGTCTACGTCATGCCCA CTGTCACCTTGCCACCCAGGGAAATTGTGGCCCTGGTCACTATGACCCCAGACCAGAGGCAGGATGTTGT CCGGCTTCTGCAGCTGCGCACAGCAGAGCCAGAGGTGCGCACCAAGCCCTACACGCTGGAGGAGTTCTCC TATGACTACTTCAGGTCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGAGGGAAGG ACCGACTGTGGAGTCACACACGAGAGCCCCTCAAGCAGGCCCTGCTCAAGAAGATCGTGGGCAGCGAGGA ACTCTCCCAGGAAGCCTGCATGTCCTTCATAGCTGTGCTCAAGTACATGGGTGACTACCCATCCAAGAGG ACACGCTCTGTCAATGAGCTCACTGACCAGATCTTCGAGTGGGCAGTGAAGGCCGAGCCCCTCAAAGATG AGGCCTACGTGCAGATCCTGAAGCAGCTGACTGACAATCATATCAGGTACAGCGAAGAGAAGGGTTGGGA GCTGCTGTGGCTGTGCACAGGCCTCTTCCCACCCAGCAACATCCTCCTGCCTCATGTTCAGCGTTTCCTG CAGTCCCGCAAACACTGTCCTCTTGCCATTGACTGCCTACAAAGGCTCCAGAAAGCCCTGAGAAACGGGT CCCGGAAGTACCCTCCACACCTGGTGGAGGTAGAGGCCATCCAGCACAAGACTACCCAGATTTTCCACAA GGTCTACTTCCCTGACGACACGGATGAGGCTTTTGAGGTAGAGTCCAGCACCAAGGCCAAGGACTTCTGC CAGAACATCGCCAGCCGGCTGCTCCTCAAGTCTTCTGAGGGATTCAGCCTTTTTGTCAAAATCGCAGATA AGGTCATCAGCGTCCCTGAGAATGACTTCTTCTTTGACTTTGTTCGACACCTGACAGACTGGATAAAGAA GGCACGGCCCATCAAGGATGGAATCGTGCCCTCACTGACCTACCAGGTGTTCTTCATGAAGAAGCTGTGG ACCACCACCGTGCCTGGCAAGGACCCCATGGCCGACTCCATATTCCACTATTACCAGGAACTGCCCAAGT ATCTCCGAGGCTACCACAAGTGCACCCGGGAGGAGGTGCTACAGCTGGGCGCGCTCATCTACAGGGTCAA GTTTGAGGAGGACAAATCCTACTTCCCTAGCATCCCCAAGTTGCTGAGGGAGCTGGTGCCCCAGGACCTA ATCCGGCAGGTCTCACCTGATGACTGGAAACGGTCTATTGTCGCCTACTTCAACAAGCATGCAGGGAAGT CCAAGGAGGAGGCCAAGCTGGCCTTCCTCAAGCTCATCTTCAAGTGGCCCACCTTTGGATCAGCCTTCTT TGAGGTGAAGCAAACGACAGAACCAAACTTCCCAGAGATTCTCTTAATTGCCATCAACAAGTATGGGATC AGCCTCATCGATCCCAGAACCAAGGACATCTTGACTACTCACCCCTTCACCAAGATCTCCAACTGGAGTA GTGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTCCGTGGGAGCAAACTGCTCTGTGAGACATC ACTGGGATACAAAATGGATGATCTTCTGACTTCCTACATCAGCCAGATGCTCACTGCCATGAGCAAACAG AGGAACTCCAGGAGCGGGAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153473
Insert Size	6534 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153473.1, NP_703203.1
RefSeq Size	6807 bp
RefSeq ORF	6534 bp
Locus ID	266714
Gene Summary	a myosin with a plus-directed motor [RGD, Feb 2006]

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