Cpd (NM_012836) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213267 representing NM_012836 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGGCTGGGATGAGCGGCCGCCCTGGCGGCTGGAAAGCCTGCGGCTGCTACCGCCGCCACCGC TGCTGCTGCTACTGCTGCTATTGAGGAGCTCGGCCCAGGCGGCGCACATCAAGAAGGCTGAGGCGACCAC GACGACCGTCGGCGGCTCCGAGGCGGCCGAGGGCCAGTTTGACCACTACTACCACGAAGCGGCGCTGGGC GAGGCACTGGAGGCGGCAGCGGCCGCGGGGCCGCCTGGCCTGGCCCGTCTCTTCAGCATCGGCAATTCGG TGGAGGGCCGACCGCTGTGGGTGCTGCGCCTCACGGCTGGCCTGGGGCCGCCGCCCACCCCCGCCGCCGT GGGGCTGGACGCCGCGGGGCCGCTGCTGCCCGGCCGACCGCAGGTGAAGCTGGTGGGTAACATGCACGGC GACGAGACGGTGTCGCGCCAGGTGTTGGTCTACCTGGCCCGTGAGCTGGCGTCGGGCTACCGCCGCGGGG ACCCGCGCCTGGTCCGCCTGCTCAACACCACCGACGTATATTTGCTGCCCAGCCTGAATCCGGATGGCTT CGAACGCGCTCGCGAGGGTGACTGCGGCCTCGGCGACAGCGGTCCGCCTGGGACCAGCGGCCGAGACAAC AGCCGAGGCCGCGACCTCAACCGCAGCTTCCCTGACCAGTTCAGCACGGGGGAGCCACCTTCCCTGGATG AGGTGCCAGAGGTGCGCGCCCTCATCGACTGGATCCGCAGGAACAAGTTTGTGCTTTCTGGAAATCTTCA TGGTGGCTCAGTGGTAGCAAGCTATCCTTTTGATGATTCTCCCGAGCATAAGACCACTGGAATCTATAGC AAAACCTCCGATGATGAAGTCTTTCGATACTTGGCGAAAGCTTATGCTTCTAACCACCCTATAATGAGAA CTGGTGAACCCCATTGTCCAGGAGATGAAGAGGAGACTTTCAAAGACGGGATCACGAACGGTGCGCACTG GTATGATGTGGAAGGTGGTATGCAAGATTACAACTATGTGTGGGCCAACTGTTTTGAGATCACATTAGAA TTATCTTGCTGCAAATACCCACCTGCTTCTCAGCTTCGACAAGAATGGGAGAACAACCGTGAGTCTTTGA TCACATTGATTGAAAAGGTCCACATTGGAATTAAAGGATTTGTTAAAGACTCAGTCACAGGCTCTGGGTT GGAGAATGCAACCATCTCAGTGGCTGGTATTAATCATAATATCACAACTGGGAGATTCGGTGATTTCCAC AGACTGCTCATTCCTGGAAGTTACAATCTTACAGCGGTTTCACCTGGGTATATGCCGTTGACTATTAATA ATATAGTGGTGAAAGAAGGACCAGCCACAGAGATAGATTTCTCCCTTCAACCAACTGTGATGTCAGTAGT GCCTGACTCGACTGAGGCTGTGACAACTCCCGGCACAGTTGCCGTTCCTAATATTCCTCCTGGAACACCA TCGTCCCATCAGCCAATTCAGCCTAAAGACTTTCACCACCACCATTTTCCTGACATGGAAATCTTCTTGA GAAGATTTGCCAATGAATATCCTAACATCACTCGCCTTTATTCTCTGGGAAAGTCCGTAGAGTCGAGAGA ACTGTATGTGATGGAGATATCTGACAACCCAGGCGTCCATGAACCAGGTGAACCAGAATTTAAATATATT GGAAATATGCATGGAAATGAAGTGGTTGGAAGAGAACTGCTATTGAATCTCATCGAATACCTCTGCAAGA ACTTTGGAACAGACCCTGAAGTAACAGATTTGGTTCGCAGCACTAGAATTCACCTCATGCCATCCATGAA TCCTGATGGGTATGAGAAGTCCCAAGAAGGAGACTCGATAAGTGTAGTTGGCAGAAACAACAGCAACAAC TTTGACCTAAACCGAAATTTCCCAGACCAGTTTGTTCCGATCACAGATCCTACCCAACCTGAAACTATCG CTGTGATGAGCTGGGTGAAGGCCTATCCGTTTGTGCTGTCGGCCAATCTGCATGGAGGTTCCTTGGTGGT TAACTACCCTTATGATGATAATGAACAAGGAGTTGCCACATACAGCAAATCACCAGATGACGCCGTGTTT CAACAAATAGCACTTTCTTATTCCAAGGAAAACTCGCAGATGTTCCAAGGTAGACCTTGCAAAGACATGT ACCTTAATGAGTATTTTCCTCATGGCATAACGAATGGAGCGAGTTGGTATAATGTCCCAGGTGGTATGCA GGACTGGAACTATTTACAGACGAATTGCTTTGAAGTAACCATCGAACTAGGTTGTGTGAAATATCCATTC GAGAAGGAACTGCCAAAGTACTGGGAGCAAAACCGGAGATCTCTAATCCAGTTTATGAAACAGGTTCATC AGGGTGTCAAAGGATTTGTTCTTGATGCCACAGATGGTAGGGGCATATTAAATGCCACCCTCAGTGTTGC TGAAATTAATCACCCAGTGACGACTTACAAAGCTGGAGATTACTGGCGTCTCTTGGTCCCAGGGACATAT AAAATCACAGCATCTGCTCGAGGGTATAACCCCGTCACCAAGAACGTGACCGTCAGGAGTGAAGGTGCTA TTCAGGTCAACTTCACACTTGTTCGATCTTCAACAGATGCAAACAATGAGTCAAAGAAAGGAAAGGGGCA TAGCACCAGCACTGATGACACCAGCGACCCAACTTCTAAGGAGTTTGAAGCACTGATCAAACACCTTTCG GCTGAGAATGGCTTGGAAGGCTTCATGTTAAGCTCGTCCTCAGACCTGGCTCTGTATCGATACCATTCCT ACAAAGACTTATCCGAGTTCCTGAGAGGACTCGTAATGAACTACCCACACATTACAAACCTTACCACTTT GGGACAGAGTGTTGAATATCGTCACATTTGGTCCCTTGAAATCTCCAATAAGCCCAACATATCAGAACCT GAAGAACCGAAGATTCGTTTTGTTGCTGGTATCCATGGAAACGCTCCAGTTGGCACTGAACTGCTTTTGG CTCTGGCAGAATTTCTTTGCCTGAATTACAAAAAGAATCCAGTTGTTACCCAATTGGTTGACAGGACTAG AATTGTGATTGTCCCCTCTCTGAATCCAGATGGGAGAGAGCGAGCACAGGAGAAAGACTGTACCTCGAAG ACTGGGCACACAAATGCCCGTGGCAGGGACCTGGACACAGACTTCACAAGTAATGCTTCCCAACCTGAGA CCAAAGCCATCATTGAAAACTTGATTCAAAAACAGGATTTCAGCCTTTCCATTGCTTTAGATGGTGGTTC TGTGCTGGTCACATATCCGTACGACAAGCCAGTCCAGACAGTGGAAAACAAAGAAACGTTAAAGCATCTG GCGTCTCTTTATGCAAATAACCACCCATCAATGCACATGGGTCAGCCCAGTTGCCCAAATAATTCAGATG AGAACATACCAGGAGGAGTGATGCGGGGAGCAGAGTGGCACAGTCACCTGGGCAGCATGAAGGATTATAG TGTCACCTATGGCCATTGCCCAGAAATCACAGTATACACAAGCTGCTGCTACTTCCCAAGTGCAGCTCAA CTCCCTGCCCTATGGGCAGAGAACAAGAAGTCTCTCCTCAGCATGCTGGTGGAGGTCCACAAAGGAGTCC ATGGGTTAGTAAAAGACAAGACTGGTAAACCAATCTCTAAAGCAGTAATTGTACTCAATGAAGGAATAAG AGTACACACAAAAGAGGGGGGTTATTTCCATGTGCTTTTAGCTCCTGGGGTCCATAACATCAATGCCATC GCTGATGGATATCAGCAACAGCATTCACAGGTCTTTGTGCATCATGATGCAGCCAGTTCTGTGGTGATAG TCTTTGATACAGATAACCGGATATTTGGTTTGCCAAGGGAACTTGTGGTAACTGTGTCAGGTGCCACCAT GTCAGCGCTGATCCTCACGGCTTGCATCATTTGGTGCATCTGCTCTATCAAGTCTAACAGACACAAAGAT GGCTTCCACCGGCTGCGGCAGCATCATGATGAATATGAAGATGAAATTCGCATGATGTCAACTGGCTCTA AGAAGTCCCTTTTAAGCCATGAGTTCCAGGATGAAACGGACACAGAGGAAGAGACATTATATTCTAGCAA ACATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012836
Insert Size	4137 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012836.2, NP_036968.1
RefSeq Size	4374 bp
RefSeq ORF	4137 bp
Locus ID	25306
UniProt ID	Q9JHW1
Gene Summary	putative carboxypeptidase D protein that may participate in peptide processing in the secretory pathway; alternatively spliced CPD-N form is induced by prolactin and is localized to the nucleus [RGD, Feb 2006]

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