Cdc42bpa (NM_053657) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mrck; Pk428
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213192 representing NM_053657 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGAGAAGTGCGTTTGAGGCAGTTGGAACAGTTTATTTTAGATGGGCCAGCTCAGACCAATGGGC AGTGCTTCAGTGTGGAGACGTTACTGGACATACTCATCTGTCTTTATGATGAATGCAATAATTCTCCATT GAGAAGAGAGAAGAACATTCTTGAATATCTAGAATGGGCTAAACCATTCACTTCTAAAGTGAAGCAAATG CGCTTACATAGAGAAGACTTCGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGTGAGGTTGCTGTAG TTAAACTGAAAAATGCAGATAAAGTATTTGCCATGAAAATTCTGAACAAGTGGGAAATGCTGAAAAGAGC TGAGACAGCGTGCTTTCGTGAAGAGAGGGATGTGTTGGTAAATGGAGACAGTAAGTGGATAACCACTCTG CACTATGCTTTTCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTACTTA CTCTGCTCAGCAAATTTGAAGATCGATTGCCAGAAGAGATGGCTCGGTTTTACTTGGCTGAGATGGTGAT AGCCATTGACTCAGTTCACCAGCTCCACTATGTTCACAGAGACATCAAACCTGACAATATATTGATGGAT ATGAATGGACATATTCGTTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTCCAGT CCTCAGTGGCAGTTGGAACTCCAGACTACATTTCCCCGGAAATCCTTCAGGCTATGGAGGACGGGAAAGG CAGATATGGACCAGAGTGTGACTGGTGGTCCTTGGGAGTCTGCATGTATGAGATGCTTTACGGAGAAACA CCATTCTATGCGGAATCTCTGGTGGAGACATATGGAAAAATTATGAATCATAAAGAGAGGTTTCAGTTTC CAACCCAAGTGACTGATGTGTCTGAAAATGCTAAAGATCTTATTCGAAGACTCATTTGTAGCAGAGAACA TCGACTTGGCCAGAATGGAATAGAAGACTTTAAGAAACATCCGTTTTTCAGTGGAATTGATTGGGATAAC ATCCGAAACTGTGAAGCACCTTACATTCCAGAAGTTAGTAGTCCAACAGACACGTCAAATTTCGATGTGG ATGACGACTGTTTAAAAAACTCTGAAACAATGCCCCCACCAACACATACTGCATTTTCTGGCCATCATCT ACCATTTGTTGGGTTTACATATACTAGTAGCTGTGTTCTTTCTGATCGGAGCTGTTTAAGAGTCACAGCA GGTCCCACCTCACTGGATCTTGACGTCAATGTCCAGAGGACTCTAGATAATAACTTAGCAACTGAAGCTT ATGAAAGAAGAATTAAACGGCTAGAACAGGAAAAGCTTGAACTTACTAGAAAACTTCAAGAGTCAACACA GACTGTTCAAGCCCTGCAGTATTCAACTGTTGATGGTCCACTAACGGCAAGCAAAGATTTAGAAATTAAA AGTCTGAAAGAAGAGATTGAAAAACTAAGAAAACAAGTAGCAGAAGTGAATCATTTGGAACAACAACTTG AAGAAGCTAATTCTGTGAGACGAGAACTAGATGATGCTTTTAGGCAAATCAAAGCTTTTGAAAAACAAAT CAAAACTTTGCAGCAGGAAAGAGAAGAGCTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC CAATCCAAAGAGCTGAAAGACGCACACTGTCAGAGGAAACTGGCCATGCAGGAGTTCATGGAGATCAATG AGCGACTAACAGAATTGCACACCCAAAAGCAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT GGACCTGGTGATGCAAAAAGCTGAGAGCTTAAGGCAGGAGCTTCGCAGAGCAGAAAGAGCCAAGAAAGAG CTGGAGGTTCACACTGAAGCTCTAATTGCTGAAGCCTCAAAGGACCGGAAGCTCCGAGAGCAGAGCAGAC ATTATTCTAAACAGCTGGAAAATGAATTGGAGGGACTGAAGCAAAAACAAATTAGTTACTCACCAGGAAT ATGCAGCATAGAACACCAGCAAGAGATAACTAAACTGAAAACTGACTTGGAAAAGAAAAGTATCTTTTAT GAAGAAGAAATCTCTAAAAGAGAAGGAATTCATGCAAGTGAGATTAAAAATCTGAAGAAGGAGCTGCATG ACTCAGAAGGCCAGCAACTTGCTCTCAACAAAGAAATTATGGTTTTAAAAGACAAATTGGAAAAAACCAG AAGGGAAAGTCAAAGTGAAAGGGAAGAATTTGAAAATGAGTTCAAGCAACAATATGAACGGGAAAAAGTA TTATTAACTGAAGAGAATAAAAAGTTAACGAGTGAACTCGATAAGCTAACTTCGTTGTATGAGAGCTTAA GTTTGCGCAACCAGCACTTAGAAGAAGAGGTCAAAGATCTAGCAGATAAGAAGGAATCAGTTGCCCACTG GGAAGCCCAAATCACAGAGATAATCCAATGGGTGAGTGATGAGAAGGACGCACGGGGCTATCTTCAGGCC TTAGCTTCTAAAATGACTGAAGAATTAGAAGCGTTAAGGAATTCCAGCCTGGGGACACGAGCGACAGATA TGCCCTGGAAAATGCGTCGTTTTGCAAAACTGGACATGTCAGCTAGACTAGAGTTGCAATCTGCCCTGGA TGCAGAAATCAGAGCTAAGCAGGCCATCCAGGAAGAGCTGAATAAAGTCAAAGCATCCAACATCATAACA GAATGTAAACTGAAAGATTCAGAGAAGAAGAACTTGGAACTGTTGTCCGAAATCGAGCAGCTGATAAAGG ACACTGAAGAGCTTAGATCTGAAAAGGGTGTAGAGCACCGAGACTCACAGCACTCTTTCTTGGCATTTTT GAATACGCCTACCGATGCTCTGGATCAATTTGAACGCTCTCCGTCCTGTACTCCAGCTGGCAAAGGCAGA CGTATTGCAGACTCTGCTCCGCTTCCAGTTCACACACCAACTTTAAGGAAAAAGGGATGCCCAGCTTCAG CTGGTTTTCCACCTAAGCGCAAGACTCATCAGTTTTTCGTGAAGTCTTTCACTGCCCCTACCAAGTGCCA TCAGTGTACCTCCTTGATGGTCGGTTTGATAAGACAGGGCTGTTCCTGCGAAGTCTGTGGGTTCTCATGT CATATAACTTGTGTGAACAAAGCCCCGACCACTTGCCCAGTTCCTCCTGAGCAAACTAAAGGCCCACTCG GTATAGACCCCCAGAAGGGAGTAGGGACAGCGTATGAGGGCCATGTCCGGATCCCTAAGCCGGCGGGAGT GAAGAAAGGGTGGCAGAGAGCACTGGCTGTGGTCTGTGACTTCAAACTGTTTCTTTATGATATCGCGGAG GGAAAAGCATCGCAGCCCAGCTCCGTCATTAGTCAAGTGATCGACATGAGAGATGAAGAATTTTCCGTGA GTTCAGTCTTGGCTTCTGATGTTATTCATGCAAGTCGAAAAGATATTCCCTGTATATTTAGAGTCACAGC CTCCCAGCTCTCAGCACCTAGTGACAAATGTTCCATCCTGATGCTAGCCGACAGCGAGACTGAGAGGAGT AAGTGGGTGGGAGTGCTGAGTGAACTCCACAAGGTTCTGAAGAAAAACAAGTTCAGAGACCGCTCAGTGT ATGTCCCCAAAGAGGCTTACGACAGCACTCTACCCCTCATTAAAACAACGCAGGCCGCGGCAATCATAGA TCATGAGCGGGTAGCTTTGGGAAATGAAGAAGGATTATTTGTTGTGCATGTCACTAAAGATGAGATTATT AGAGTTGGTGATAATAAGAAAATTCATCAGATTGAACTCATTCCAAGTGACCAGCTTGTTGCTGTGATCT CAGGCCGAAATCGCCACGTCCGACTTTTCCCTATGTCAGCTTTGGATGGGCGAGAGACGGATTTTTATAA ACTGGCAGAGACTAAAGGGTGTCAAACCATAGCTGCTGGGAAGGTGCGCCATGGAGCTCTGTCCTGCCTG TGTGTGGCCATGAAAAGACAGGTCCTCTGCTATGAACTGTTCCAAAGCAAGACCAGGCACAGGAAGTTTA AGGAGATTCAAGTCCCATGTAATGTCCAGTGGATGGCCATCTTCAGTGAACATCTCTGTGTGGGATTCCA GTCAGGATTTCTCAGATACCCCCTGAATGGGGAAGGAAGTCCATGCAATATGCTCCATTCAAATGACCAC ACACTGGCATTCATTACACACCAACCAATGGATGCTATCTGTGCCGTTGAGATCTCCAACAAAGAGTATT TGCTGTGTTTTAGCAGCATTGGGATATACACGGACTGCCAGGGCCGAAGATCTAGACAGCAGGAACTGAT GTGGCCAGCAAATCCTTCCTCCTGTTGTTACAATGCACCATATCTCTCCATTTACAGTGAAAATGCAGTT GATATCTTTGATGTAAACTCCATGGAATGGATACAGACGCTTCCTCTCAAGAAGGTTCGACCTTTAAACA CTGAAGGATCATTAAATCTTTTAGGGTTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGA AGGGGATGAACTTGTAGTTCCTGAAACATCAGATAATAGCCGAAAACAGATGGTTAGAAACATCAACAAC AAACGGCGTTACTCCTTCCGGGTCCCAGAAGAAGAGAGGATGCAGCAGAGAAGGGAGATGCTACGAGATC CAGAAATGAGAAATAAATTAATTTCTAACCCAACTAATTTTAACCACATAGCACACATGGGTCCTGGAGA TGGAATACAGATCCTAAAAGACCTGCCCATGAACCCACGGCCTCAGGAAAGCCGGACAGTATTCAGTGGC TCCGTCAGTATTCCCTCCATCACCAAGTCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCCAGCAGTGGCC TGTCTGCACGGTCATCAGCCCAGAATGGCAGTGCGCTAAAGAGGGAATTCTCTGGAGGAAGCTACAACAC GAAGCGACAGCCCATGCCCTCTCCCTCAGAAGGCTCTTTGTCATCAGGAGGCGTGGACCAAGGAAGTGAC GCCCCCGTGAGGGACTATGATGGAGAGGACTCTGATTCTCCAAGGCATTCCACAGCTTCCAACAGCTCTA ACCTGAGCAGCCCCCCAAGCCCCGTTTCACCCCGAAAGACCAAGAGCCTCTCCTTGGAGAGCACAGACCG TGGGAGCTGGGATCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053657
Insert Size	5199 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053657.1, NP_446109.1
RefSeq Size	6335 bp
RefSeq ORF	5199 bp
Locus ID	114116
UniProt ID	O54874
Gene Summary	catalyzes the phosphorylation of Ser19 of nonmuscle myosin light chain; binds GTP-Cdc42 and plays a role in cytoskeleton organization [RGD, Feb 2006]

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