Usp30 (NM_001107153) Rat Untagged Clone
CAT＃: RN213092
Usp30 (untagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug)
CNY 6650.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213092 representing NM_001107153
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGTTCCCGGGCGCAGGCGGCGAGGACAGCGGCCGACAAGGCCCTCCAGCGCTTCCTGCGCACCG GGGCGGCTGTCAGATACAAAGTCATGAAGAACTGGGGAGTGATCGGTGGAATTGCTGCTGCTCTTGCAGC AGGAATATATGTTATCTGGGGTCCCATTACAGAGAGGAAGAAGCGGAGAAAAGGGCTTGTGCCTGGCCTT GTCAACTTGGGGAACACCTGCTTCATGAACTCCCTGCTGCAGGGTTTGTCTGCCTGCCCCGCGTTTGTCA AGTGGCTGGAGGAGTTTACCACCCAGTACTCCAGGGACCAGCAGGGGCCGCACACACACCAGTGCCTGTC CTTGACACTGCTGAGCCTCCTGAAAGCTCTGTCCTGCCAAGAAGTCACTGAGGAAGAGGTCTTGGACGCC AGCTGCCTGCTGGACGTCCTGAGAATGTACCGGTGGCAGATCTCGTCCTTTGAGGAGCAGGATGCTCACG AACTGTTCCATGTCATCACCTCATCCCTGGAGGACGAGCGGGACCGCCAGCCTCGGGTCACCCACTTGTT TGATGTGCACTCCCTGGAGCAGCAGTCAGAAATGGCTCCCAGACAAGTCACCTGCCACACGAGAGGGTCC CCTCACCCCACGACCAATCCCTGGAAGTCTCAGCACCCCTTCCATGGGAGGCTCAGCAGTAACATGGTGT GCAAGCACTGTGAGCACCAGAGCCCTGTTCGGTTTGACACCTTTGATAGCCTTTCCCTCAGTATTCCAGC TGCTACTTGGGGGCACCCACTGACACTGGACCACTGCCTTCACCACTTCATCTCATCCGAGTCGGTGCGA GACGTTGTGTGTGACAACTGTACAAAGATTGAAGCAAAAGGGACCCAGAACGGGGAAAAGGTGGAGCACC AGAGGACCACTTTCGTTAAACAGTTAAAGCTAGGAAAGCTGCCACAGTGCCTCTGCATCCACCTGCAGCG GCTCAGCTGGTCCAGCCAAGGCACGCCCTTGAAGCGGCATGAGCACGTGCAATTTAACGAGTTCCTGATG ATGGACTTCTACAAGTACCGCCTCCTGGGACATAAACCCAGCCAGCATGGCCCCAAAGCCACTGAGAGCC CAGGGTCTGCCCTGGGAGTACAGGACACACAGGCGGCCCCGAAGCCAGGTCTGAGTCAGCCAGCGGCCCC CAAGACACAGTTTTTTATGAATGGCGCCTGCTCCCCGTCTTTGTTGCCAGCCCTGCCTTCTCCGATGGCT TTCCCTCTACCAGTGGCTCCTGACTACAGCTCCTCCATGTACCTCTTCCGCTTGATGGCAGTTGTCGTCC ACCATGGAGACATGCACTCTGGACACTTTGTCACCTACAGACGGTCACCACCTTCTGCCAAGAACCCTCT CTCAACCAGCAACCAGTGGCTGTGGATTTCCGATGACACTGTCCGAAAGGCCAGCCTGCAGGAGGTCCTG TCCTCTAGTGCCTACCTGCTGTTCTATGAGCGAGTTCTGTCCAGGGTGCAGCAGCAGGGGCGAGAGTACA GGTCAGAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001107153 |
| Insert Size | 1554 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001107153.1, NP_001100623.1 |
| RefSeq Size | 3362 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 304579 |
| UniProt ID | D3ZPG5 |
| Gene Summary | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:24896179). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213092 | Usp30 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 5776.00 |
|
| RR213092L3 | Lenti ORF clone of Usp30 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 8550.00 |
|
| RR213092L4 | Lenti ORF clone of Usp30 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 9310.00 |
