Nup153 (NM_001100470) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NUCZINK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213083 representing NM_001100470 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGGAGCCGGAGGCATAGGAGGGGGCGGCGGCGGCGGCAAGATCCGGACGCGTCGTTGCCACC AGGGGCCGGTGAAGCCTTACCAGCAGGGGCGACCCCAACACCAGGGCATTCTTAGCAGGGTTACAGAATC TGTTAAGAATATTGTACCAGGGTGGCTACAGAGATACTTCAACAAGAGTGAAAATGCCTGTAGCTGTTCA GTAAATGCAGATGAAGTTCCACGTTGGCCGGAAAATAGAGAAGATGAGCGTGAGATTTATGTGGATGAGA ACACAAACACTGATGACGGGAGAACCACCCCTGAGCCCACAGGCAGTAACACAGAAGAACCATCAACCAC CAGTACTGCTTCAAATTATCCAGATGTATTAACAAGACCTTCTCTTCATCGGAGTCATCTGAATTTCTCC GTGTTAGAATCCCCTGCTTTGCACTGTCAGCCATCCACGTCCTCTGCATTCCCAATCGGCAGTTCTGGAT TTTCCCTTGTGAAGGAGATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGG GTTCTCTTCAAGAGCTTCTGAGAAAGATATAGCTGTTTCAAAGAACACTTCCCTGCCACCTTTGTGGTCC CCCGAAGCAGAGCGTTCTCATTCACTCTCACAACATACTGCCATCAGCTCCAAAAAACCAGCATTCAACT TGTCTGCCTTTGGAACACTTTCCACCTCACTTGGGAATTCTTCAATTCTTAAAACAAGTCAACTTGGAGA CTCTCCTTTTTATCCTGGCAAAACAACATATGGTGGGGCAGCTGCTGCTGTAAGACAGAATAAAGTACGA AGTACACCTTACCAGGCTCCAGTTCGAAGACAGATGAAAGCCAAGCAGCTCAATGCACAGTCTTATGGTG TGACCAGTTCAACAGCACGGCGGATATTGCAGTCATTAGAAAAGATGTCAAGCCCTCTTGCGGATGCAAA AAGAATTCCATCTGCTGTTTCTTCTCCTCTGAATTCTCCCCTTGATAGGAGTGGGATAGATAGCACAGTT TTTCAGGCAAAAAAGGAAAAGGTGGACTCTCAGTATCCCCCTGTTCAGAGACTAATGACCCCAAAGCCAG TTTCCATAGCAACAAATAGAACTGTCTATTTTAAACCATCTCTGACCCCATCTGGTGACTTAAGGAAGAC TAATCAAAGGATAGATAAAAAGAACAGTACTGTAGATGAAAAAAATATCTCAAGACAAAATAGAGAGCAA GAAAGTGGCTTTTCATACCCCAATTTCAGTATTCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTGGTG GAGGTGGCAAGATGAGAAGAGAGAGAACAACACATTTTGTTGCTTCTAAACCTTCAGAGGAGGAGGAAGT AGAAGTACCATTGTTACCGCAAATCTCTCTACCAATCAGCAGTTCTTCACTGCCCACCTTCAACTTTAGT TCCCCTGCGATCTCGGCTGCTTCTTCGTCATCAGTCAGCCCTTCTCAGCCATTATCAAACAAGGTACAGA TGACCTCTCTGGGCAGCACAGGCAATCCCGTGTTCACCTTTTCCTCTCCCATTGTGAAGTCCACGCAGGC AGACGTGCTACCTCCAGCATCTATTGGATTTACGTTTAGTGTGCCTCTTGCAAAAACAGAACTTTCTGGC CCTAATAGTTCTTCAGAAACAGTTTTAAGTAGTTCAGTTACAGCTCAAGATAACACTGTAGTGAATAGTT CAAGCTCTAAGAAGCGAAGTGCCCCTTGTGAGGACCCTTTTACACCTGCAAAGATCCTGAGAGAAGGAAG TGTTTTAGACATTCTGAAAACGCCTGGTTTCATGTCACCAAAGGTTGATTCTCCTGCTCTTCAGCCCACA ACTACAAGCTCAATAGTTTATACAAGACCAGCAATAAGTACTTTTTCTTCTAGTGGGGTTGAGTTTGGAG AAAGTTTAAAAGCTGGATCATCATGGCAGTGTGACACATGTCTACTCCAGAACAAAGTCACAGACAATAA GTGTATAGCCTGTCAAGCAGCAAAACTGCCACTGAAAGAAACTGCTAAACAGACTGGAATTGGGACACCA AGCAAAAGTGACAAGCCAGCTTCTACATCAGGGACAGGTTTTGGAGATAAATTTAAACCAGCAATAGGAA CTTGGGATTGTGATACCTGTTTAGTGCAAAATAAGCCTGAAGCAGTAAAATGTGTAGCCTGTGAAACACC CAAACCTGGCACTGGTGTGAAGCGAGCCCTTCCATTGACGGTGGCTTCAGAAAGTCCTGTGACTGCTTCC TCTTCTACCACCGTGACCACTGGTACACTAGGATTTGGAGATAAATTCAAAAGGCCCGTGGGATCTTGGG AGTGTCCGGTATGCTGTGTTTCTAATAAGGCAGAAGACAGCAGATGTGTCTCCTGTACATCTGAGAAACC AGGTTTGGTATCTGCCTCAAGCAGCAACCCTGTCCCTGTCTCTCTGCCTTCTGGAGGCTGTTTAGGATTG GACAAGTTTAAAAAACCAGAGGGAAGCTGGGACTGTGAAGTATGCCTAGTGCAAAATAAAGCGGACTCTA CCAAGTGCATAGCTTGTGAAAGTGCAAAGCCAGGCACAAAGTCTGAGTTTAAAGGCTTTGGGACATCTTC ATCTTTAAATCCAGCACCCTCAGCCTTCAAATTTGGTATTCCATCATCATCTTCTGGGCTTTCTCAAACT TTTACAAGCACTGGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAATTAGGCACTTCATCTGACTCTG GGTCTACAAACACCATGAATACAAACTTTAAATTTCCTAAACCAACTGGAGATTTTAAATTTGGAGTATT ACCTGATTCCAAACCTGAAGAAATAAAAAACGACAGCAAGAATGATAATTTTCAGTTTGGACCTTCTTCT GGCTTAAGCAATCCAGCGTCTTCAGCTCCATTTCAGTTTGGGGTATCTACTCTTGGGCAGCAGGAAAAGA AAGAGGAGCTGCCTCAGTCTTCATCTGCAGGCTTCAGTTTTGGTGCAGGTGTTGCTAACCCCTCTAGTGC TGCTATTGACACCACAGTGACCTCAGAGAACAAGAGCGGCTTCAACTTTGGAACCATAGACACTAAGAGT GTCTCAGTGACTCCTTTCACGTACAAGACAACAGAAGCAAAGAAAGAAGACGCGTCTGCTACCAAGGGTG GGTTCACATTTGGTAAAGTGGACTCTGCTGCTTTGTCATCTCCCTCAATGTTTGTGTTGGGAAGGACAGA GGAGAAACAGCAAGAACCTGTTACTTCTACTTCTCTGGTATTTGGAAAGAAAGCTGACAATGAGGAGCCA AAGTGTCAACCAGTATTTTCCTTTGGAAATTCAGAGCAAACCAAAGACGAGAGTTCCTCCAAGCCGACCT TCAGTTTCAGTGTGGCAAAACCATCTGTGAAGGAGTCTGATCAGCTGGCCAAGGCTACTTTTGCATTTGG AAATCAAACCAATACAACAACTGATCAAGGTGCAGCAAAGCCAGCTTTTAGCTTCTTGAACAGCAGTTCC TCTAGTTCAAGTACGCCGGCCACTTCATCCAGTGCTAGCATATTTGGTAGTTCCACCTCTTCCTCCAGCC CGCCTGTGGCAGCCTTTGTGTTTGGACAGGCCAGTAATCCTGTCAGCAGCTCTGCCTTCGGAAACTCCGC CGAGTCCAGTACATCTCAGCCTTTGCTCTTTCCTCAAGATGGCAAACCAGCCACCACGTCCAGCACTGCC TCAGCTGCCCCTCCATTTGTATTTGGAACAGGAGCCAGTAGTAATAGTACTGTATCTTCTGGGTTCACTT TTGGGGCTACAACCACATCAAGCTCTTCAGGATCCTTCTTTGTATTTGGCACTGGACATTCAGCACCATC TGCCAGTCCAGCATTTGGTGCTAACCAGACTCCAACATTTGGACAAAGTCAAGGTGCCAGCCAGCCTAAC CCTCCAAGCTTTGGCTCTATATCGTCTTCAACAGCATTATTTTCTGCTGGTTCTCAGCCTGTACCACCAC CTACATTTGGGACAGTGTCAAGCAGCAGCCAACCTCCTGTGTTTGGACAGCAGCCTAGTCAGTCTGCATT TGGCTCTGGGACAGCCAATGCTAGTTCGGTTTTCCAGTTTGGCAGCAGCACTACAAATTTCAACTTCACA AACAATAATCCATCAGGAGTGTTCACATTTGGTGCAAGTCCCAGCACGCCTGCAGCCGCAGCCCAGCCCT CAGGTTCGGGGGGCTTTTCATTCAGCCAGTCTCCAGCATCATTTACAGTGGGGTCAAATGGGAAAAATAT GTTCTCTTCTTCCGGAACTTCAGTTTCTGGTCGCAAGATAAAGACTGCTGTTAGACGCAAGAAATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001100470
Insert Size	4407 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100470.1, NP_001093940.1
RefSeq Size	6224 bp
RefSeq ORF	4407 bp
Locus ID	25281
Gene Summary	nuclear pore complex protein; may act to to gate transcribable genes to nuclear pore complexes [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.