Arhgap29 (NM_001009405) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	B130017i01rik; RGD1306185
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212783 representing NM_001009405 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAAGCATCAGAACCTCAATTCTGTAGACCTTCAGAACGCTGCAGAAACGCTCGCTTCGAAAGTGA AAGCTGTGAACTTTACAGAAGTTAATGACGAAAACAAAAACGATCTATTTCGAGAAGTCTTTTCCTCCAT TGAAACTCTGGCATTTACCTTTGGAAACATCCTCACAAACTTCCTTATGGGAGATGTAGGCAATGATTCG ATACTACGACTGCCTATTTCTCGAGAGAGTAAGTCCTTTGAAAACATTTCTATGGACTCAGTGGACTTAC CTCATGAAAAAGGAAACTTTTCTCCTATAGAACTAGACAACTTGCTGTTAAAGAACTCTGACTCTATAGA ACTGGCTTTGTCTTATGCTAAAACTTGGTCAAAATATACCAAGAACATAGTGTCCTGGGTTGAAAAAAAG CTCAACTTGGAATTGGAGTCCACTAGAAATATTGTAAAATTGGCAGAGGCAACTAGATCTAGCATTGGAA TACAGGAGTTTATGCCTTTGCAGTCTCTCTTTACCAACGCTCTCCTTAATGATATACACAGCAGTCACCT TTTACAACAGACAATCGCAGCTCTCCAAGCCAACAAATTCGTGCAGCCTCTACTTGGGAGGAAGAATGAA ATGGAGAAACAAAGGAAAGAAATAAAAGAACTTTGGAAGCAGCAGCAGAATAAATTGCTTGAAACAGAGA CGGCTCTTAAAAAGGCAAAACTGTTATGCATGCAACGGCAAGATGAATACGAAAAGGCAAAATCATCCAT GTTTCGAGCAGAAGAAGAGCAGCTGAGTTCAAGTGTTGGACTGGGAAAAAATCTCAACAAACTACTAGAA AAAAGGCGGAGATTGGAAGAAGAGGCGCTTCAAAAAGTAGAAGAAGCAAATGAGCACTACAAAGTCTGTG TAACAAATGTTGAAGAAAGACGGAATGATCTAGAAAATACGAAGCGAGAAATTTTAACACAGCTCCGGAC ACTTGTTTTCCAGTGTGACCTTACACTTAAAGCTGTAACAGTTAACCTCTTTCATATGCAGCAGCTTCAG GCTGCTTCCCTCGCCAACAGTTTACAGTCCCTCTGTGACAGTGCCAAACTCTATGATCCAGGTCAGGAGT ATAGTGAATTCGTGAAGGCTACAAGCTCAAGTGAATTAGAAGAAAAGGTTGACGGGAATGTAAACAAACA AATAGCCAGCAGTCCGCAGACATCTGGATATGAACCTGCTGACTCTTTAGAGGATGTTGCACGCCTCCCT GACAGCTGTCACAAACTTGAAGAGGACAGGTGTTCCAACAGTGCAGACATGACAGGTCCTTCTTTCATAA GATCGTGGAAGTTCGGGATGTTTAGTGACTCTGAGAGTACTGGAGGAAGCAGTGAGTCTAGATCTCTGGA TTCAGAATCTATAAGTCCAGGAGACTTTCATCGGAAACTTCCACGAACTCCATCCAGTGGAACCATGTCT TCTGCTGATGATCTAGATGAACGAGAGCCACCATCACCTTCAGAAGCTGGACCCAATTCCCTCGGAACAT TTAAGAAAACTTTGATGTCGAAGGCAGCTCTCACTCACAAGTTTCGCAAGTTGAGATCCCCCACAAAGTG CAGGGATTGTGAAGGCATCGTAATGTTCCCGGGCGTCGAGTGTGAAGAGTGTCTCCTTGTTTGTCACCGA AAGTGTTTGGAGAATTTAGTCATTGTTTGTGGTCATCAAAAACTTCAGGGGAAAATGCACATATTTGGAG CAGAATTCATACAAGTTGCAAAAAAGGAACCAGATGGCATCCCTTTCGTGCTAAAAATATGCGCCTCAGA AATTGAAAGTAGAGCCTTGTGTCTCCAGGGAATTTATCGTGTTTGTGGAAACAAAATAAAAACTGAAAAA CTGTGCCAAGCTTTGGAAAATGGAATGCACTTAGTAGACATTTCAGAATTCAGTTCACATGACATCTGTG ATGTCTTGAAATTATACCTGCGACAGCTTCCAGAACCATTTATTTTATTCAGATTGTACAAGGAATTTAT AGACCTTGCAAAAGAGATACAACATGTAAATGAAGAACAAGAGGCAAAAAAAGATAGCCCCGAAGACAAA AAGCACCCACACGTGAGCATAGAAATCAACCGCATCCTTCTGAGGAGCAAGGACCTGCTGCGACAGCTGC CAGCATCAAACTTCAACAGTCTTCATTACCTCATCGTCCATCTGAAGCGGGTGGTGGACCATGCAGAAGA GAACAAGATGAATTCTAAGAACTTGGGGGTGATATTTGGCCCAACGCTCATTAGGCCAAGGCCTACAACG GCTCCTGTCACCATCTCGTCCCTTGCTGAATATTCCAGTCAGGCACGAGTAGTAGAGTTCCTCATTACTT ACGCACAGAAGATCTTCGATGGGTCCCTCCAGCCTCAAGCTGGTGTCATAGCTAACACAGGTGCTATTGC ACCTCAAGTCGATCACGGGTGTCATCCAAAACCGCTCTTATCACCAGATGAGAGAGACTCGGATCATTCT TTGAAACAACTATTCTTCTCTTCAAAGGAAGATATCCGTACTATGGATTGTGAGAGCAAAACTTTTGAAT TAACTACATCATTTGAAGAATCAGAACGCAAACAAAATGCATTGGGAAAATGTGATGCTCCCATCCTTGA TAACAAAGTACATTTGCTTTTTGACCAAGAGCTTGAGTCAGCATCCCACAAGACGGAAGATACCTGTAAA AGCCCTAAGCTGCTCCTTCTGAGATCTGATAGGGTAGCAAATAGTGTGCAGAGACCGACTCCGAGGACCA GGCTAAGACCTGTAAGTCTGCCTGTGGATCGACTGCTGCTGCTTGCCGGGTCTCCTACTGAGAGAAGCAG CCGGAATACAGGAAACACAGACTCAGACAAGTTTGGCAAGAATGCCGCCTTTGAAGGACTCCATAGAAAG GACAACTCAAATACTACTTGTTCCAAAGTTAATGGCTTTGACCAGCAAAATGTACAGAAATCCTGGGACA AACAGAATGAGCGGAACAGTTTCACTGCCAAGACTACGGTGATTATCCCCAGTGCCTATGCCGAGAAGGG ATTGGCAGTGAGCACGGGGAACAACAGGGGCCATTCCAGTGGTGCTGCCCAGCCTAGTAAAGCACATGCA GACCCCGCCAGGTCTGCAAGAGACACGTCCGAGCACAGCTCCTCTGACTCCTGCCCTGTTGCTGCTGTCA GAGCACCCAGAACACTGCAGCCCCAGCACTGGACAACGTTTTACAAACCACCTAACCCCACCTTCAATGT CAGGGGCACTGAGGAGAAAACAGCATTCCCCTCAGCAGCTGTGCCTCCTGTCCTGGTGCATGCTCCCCAG AGTCATGTGGCAAAGTCAGACCCAGATTTGGAGGCCACATTGGCTTGTCCTGTGCAGACAAGTGGTCAAC CTAAAGAGAGCTCTGAGGAGCCCGGCCTGCCTGAGGGGACTCCAACCTGCCAGAGACCTCGACTAAAACG AATGCAGCAGTTTGAAGACCTTGAAGATGAAATCCCACAGTTTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001009405
Insert Size	3549 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001009405.1, NP_001009405.1
RefSeq Size	4076 bp
RefSeq ORF	3549 bp
Locus ID	310833
UniProt ID	Q5PQJ5
Gene Summary	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity). In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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