Ptch1 (NM_053566) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ptch; Ptch2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211471 representing NM_053566 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGCTGGTAACGCCGCCGGGGCCCTGGGCAGGCAGGCCGGCGGCGGGAGGCGCAGACGGACCG GGGGACCGCACCGCGCCGCACCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGC TCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAA AGACTCTTATTTAAACTGGGTTGTTACATTCAAAAGAACTGCGGCAAGTTTTTGGTTGTGGGTCTCCTCA TATTTGGGGCCTTCGCTGTGGGATTAAAGGCAGCTAATCTCGAGACCAACGTGGAGGAGCTGTGGGTGGA AGTTGGTGGACGAGTGAGTCGAGAATTAAATTATACCCGCCAGAAGATAGGAGAAGAGGCTATGTTTAAT CCTCAACTCATGATTCAGACTCCAAAAGAAGAAGGCGCTAATGTCCTGACCACAGAGGCCCTACTACAAC ACCTGGACTCAGCACTCCAGGCCAGCCGTGTGCACGTCTACATGTATAACAGGCAATGGAAGTTGGAACA TTTATGCTACAAATCAGGGGAACTTATCACAGAGACAGGCTACATGGATCAGATAATAGAATACCTTTAC CCTTGCTTAATCATTACACCTTTGGACTGCTTCTGGGAAGGGGCAAAGCTACAGTCCGGGACAGCGTACC TCCTAGGTAAGCCTCCTTTACGGTGGACAAACTTTGACCCCTTGGAATTCCTAGAAGAGTTAAAGAAAAT AAACTACCAAGTGGACAGTTGGGAGGAAATGCTGAATAAAGCCGAAGTTGGCCATGGGTACATGGACCGG CCTTGCCTCAATCCAGCCGACCCAGATTGCCCTGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTG ATGTGGCCCTTGTTTTGAACGGTGGATGTCAAGGTTTATCCAGGAAGTATATGCATTGGCAGGAGGAGTT GATTGTGGGTGGCACCGTCAAGAACGCCACTGGAAAGCTTGTCAGCGCTCATGCCCTGCAAACCATGTTC CAGTTAATGACTCCCAAGCAAATGTATGAACACTTCAGGGGCTACGACTATGTCTCTCACATCAACTGGA ATGAGGATAGGGCTGCCGCCATCCTGGAGGCATGGCAGAGGACTTACGTGGAGGTGGTTCATCAAAGTGT TGCCCCAAACTCCACTCAAAAGGTGCTTTCCTTCACCACCACGACCCTGGACGACATCCTAAAATCCTTC TCTGATGTCAGTGTCATCCGAGTGGCCAGTGGCTACCTACTGATGCTTGCCTACGCCTGTTTAACCATGC TGCGCTGGGACTGCTCCAAGTCCCAGGGTGCCGTGGGGTTGGCTGGTGTCCTGTTGGTTGCACTGTCAGT GGCTGCAGGATTGGGCCTCTGCTCCTTGATTGGCATTTCCTTTAATGCTGCAACAACTCAGGTTTTGCCA TTTCTTGCCCTTGGTGTTGGTGTGGACGACGTCTTTCTCCTGGCCCATGCATTCAGTGAAACGGGACAGA ATAAGAGGATTCCATTTGAGGACAGGACTGGGGAGTGCCTCAAGCGCACGGGAGCCAGCGTGGCCCTCAC CTCCATCAGCAATGTCACTGCCTTCTTCATGGCTGCATTAATCCCTATTCCTGCACTGCGAGCGTTCTCC CTCCAGGCTGCTGTGGTGGTGGTATTCAATTTTGCCATGGTTCTACTCATTTTCCCTGCAATTCTCAGCA TGGATTTATACAGACGTGAGGACAGAAGATTGGATATTTTCTGCTGTTTCACAAGCCCCTGTGTCAGCCG GGTGATTCAAGTTGAACCGCAGGCCTACACAGAGCCTCATAGTAACACCCGGTACAGCCCCCCACCCCCG TACAGCAGCCACAGCTTCGCCCATGAAACCCACATCACCATGCAGTCCACCGTTCAGCTCCGCACAGAAT ATGACCCTCACACACACGTCTACTACACCACCGCCGAGCCGCGCTCTGAGATCTCTGTACAGCCTGTCAC CATCACCCAGGACACCCTCAGCTGTCAGAGCCCTGAGAGCACCAGTTCCACCCGGGACCTGCTTTCTCAA TTCTCTGACTCCAGCCTCCACTGCCTTGAGCCCCCCTGCACCAAGTGGACACTTTCTTCGTTTGCAGAGA AGCACTACGCTCCTTTCCTCTTGAGACCCAAAGCCAAGGTTGTGGTAATCCTTCTTTTCCTGGGCTTGCT GGGCGTCAGCCTTTATGGGACCACCCGAGTGAGAGACGGGCTGGACCTCACGGACATTGTCCCCCGGGAA ACCAGAGAATATGACTTCATAGCTGCCCAGTTCAAGTACTTCTCTTTCTACAACATGTATATAGTCACCC AGAAAGCAGACTACCCAAATATCCAACACCTGCTTTACGACCTTCATAAGAGTTTCAGCAGTGTGAAGTA TGTCATGCTGGAGGAGAACAAGCAACTTCCCCAAATGTGGCTGCATTACTTTAGAGACTGGCTCCAAGGA CTTCAGGATGCATTTGACAGTGACTGGGAAACCGGGAGGATCATGCCAAACAATTATAAAAATGGATCTG ATGATGGGGTCCTCGCTTACAAACTCCTGGTGCAGACTGGCAGCCGAGACAAGCCCATCGACATTAGTCA GTTGACTAAACAGCGTCTGGTGGACGCAGATGGCATCATTAATCCGAGCGCTTTCTACATCTACCTGACC GCTTGGGTCAGCAATGACCCTGTAGCTTACGCTGCCTCCCAGGCCAACATCCGGCCTCACAGACCAGAGT GGGTGCACGACAAAGCTGACTACATGCCAGAGACCAGGCTGAGAATCCCAGCAGCAGAGCCCATAGAGTA CGCTCAGTTCCCTTTCTACCTCAACGGCCTACGGGACACCTCAGACTTTGTGGAAGCCATAGAAAAAGTG AGAGTCATCTGTAACAACTACACGAGTCTGGGGCTGTCCAGCTACCCCAATGGCTACCCCTTCCTGTTCT GGGAGCAGTACATCAGCCTGCGTCACTGGCTGCTGCTAGCCATCAGCGTGGTGCTGGCCTGCACATTTCT AGTGTGCGCAGTCTTCCTCCTGAACCCCTGGACGGCCGGGATCATTGTCATGGTCCTGGCTTTGATGACC GTTGAGCTCTTCGGCATGATGGGCCTCATTGGGATCAAGCTGAGTGCTGTGCCGGTGGTCATCCTGATTG CGTCTGTTGGCATTGGAGTAGAATTCACCGTCCACGTGGCTTTGGCCTTTCTGACAGCCATTGGGGACAA GAACCACAGGGCTATGCTCGCCCTGGAGCACATGTTCGCTCCTGTTCTGGACGGTGCTGTGTCCACTCTG CTGGGTGTACTGATGCTTGCGGGATCCGAATTTGACTTCATTGTCAGATACTTCTTTGCCGTCCTGGCCA TCCTCACCGTCTTGGGAGTTCTCAACGGACTGGTTCTGCTGCCTGTACTCTTATCCTTCTTCGGACCATG TCCTGAGGTGTCTCCAGCCAACGGCCTAAACCGGCTGCCCACTCCTTCGCCTGAGCCGCCCCCAAGCGTC GTCCGGTTTGCCGTGCCTCCTGGTCACACGAACAATGGGTCTGATTCCTCCGACTCCGAGTACAGCTCTC AGACCACGGTGTCTGGCATCAGTGAGGAGCTCAGGCACTATGAAGCACAGCAGGGCACCGGAGGTCCTGC CCACCAAGTGATTGTGGAAGCCACAGAAAACCCCGTCTTTGCCCGGTCCACTGTGGTGCATCCTGACTCC AGACATCAGCCTCCCTTGAACTCTCGGCAACAGCCCCACCTGGACTCTGGCTCCCTGTCCCCTGGACGGC AAGGCCAGCAGCCTCGAAGGGATCCCCCCAGAGAAGGCTTGCGGCCACCCCCCTACAGACCGCGCAGAGA CGCTTTTGAAATTTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGACCGCTCGGGCCCCCATGGGGCC CGTTCTCACAACCCTCGGAACCCAACGTCCACTGCCATGGGCAGCTCTGTGCCCAGCTACTGCCAGCCTA TCACCACTGTGACGGCTTCCGCTTCTGTAACTGTCGCTGTGCACCCCCCACCTGGACCTGGGCGCAACCC CCGAGGGGGACCCTGTCCAGGCTACGAGAGCTACCCCGAGACTGATCACGGGGTGTTCGAGGATCCCCAT GTGCCTTTTCATGTCAGGTGTGAGAGGAGGGACTCGAAGGTGGAGGTTATAGAACTACAGGACGTGGAGT GTGAGGAGAGGCCGTGGGGGAGCAGCTCCAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053566
Insert Size	4305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053566.1, NP_446018.1
RefSeq Size	4305 bp
RefSeq ORF	4305 bp
Locus ID	89830
Gene Summary	human homolog acts as a receptor for sonic hedgehog [RGD, Feb 2006]

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