Pip4p1 (NM_001014233) Rat Untagged Clone

CAT#: RN211036

Tmem55b (untagged ORF) - Rat transmembrane protein 55B (Tmem55b), (10 ug)



  "NM_001014233" in other vectors (3)

CNY 3990.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1307475; Tmem55b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211036 representing NM_001014233
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGACGGAGAGCGCTCCCCGCTGCTCTCCGAGGCCGGCGATGGCGGCGCTGGTGGCAACGGCT
TAGCGGGTCCGGGCGGGAGCGCGACTGGGCCCGGTGGAGGCCTGACGCCCTCCGCACCTCCGTACGGAGC
CGGTAAACATGCCCCGCCCCAGGCATTTCCCCCGTTCCCTGAGGGGCATCCAGCTGTGTTGCCTGGAGAG
GACCCACCCCCCTACTCACCCCTGACTAGCCCGGATAGTGGGAGTGCCCCCATGATTACCTGTCGAGTCT
GTCAGTCTCCGATCAACGTGGAAGGCAAGATGCATCAGCATGTAGTCAAATGTGGTGTCTGCAACGAAGC
CACCCCGATCAAGAATGCACCTCCAGGAAAGAAGTATGTCCGATGTCCCTGTAACTGTCTCCTTATCTGC
AAAGTGACTTCTCAGCGGATTGCCTGCCCTCGGCCCTACTGCAAAAGAATCATCAACCTAGGGCCTGTAC
ATCCAGGACCTCTGAGTCCAGAACCACAGCCAATGGGTGTCAGGGTCATCTGTGGACATTGCAAGAATAC
GTTTCTGTGGACAGAATTCACAGACCGAACCTTGGCACGATGCCCTCACTGCAGAAAAGTGTCATCTATT
GGGCGCAGATATCCTAGGAAGAGATGCATTTGCTGCTTCTTACTTGGTTTACTCTTGGCAGTCACTGCCA
CTGGCCTTGCTTTTGGCACATGGAAGCCTGCACAGCAATATGGAGGTATCTATGCAGCCTGGGCGTTTGT
CATTTTGTTGGCCGTTCTATGTTTGGGCCGGGCCCTTTACTGGGGCTGTATGAAGGTCAGCCATCCTGTC
CAGAACTTCTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001014233
Insert Size 855 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001014233.2, NP_001014255.1
RefSeq Size 1657 bp
RefSeq ORF 855 bp
Locus ID 364298
UniProt ID Q5PPM8
Gene Summary Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P) (By similarity). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity). Regulates lysosomal positioning by recruiting JIP4 to lysosomal membranes, thus inducing retrograde transport of lysosomes along microtubules (By similarity). Contributes to assembly of the V-ATPase complex in lipid rafts of the lysosomal membrane and to subsequent amino acid-dependent activation of mTORC1 (By similarity). May play a role in the regulation of cellular cholesterol metabolism (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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