Ankrd12 (NM_001108238) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210822 representing NM_001108238 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAATCTGGGTTCACAAAACCAGTGCAGAGTGAAAATTCTGACAGTGACAGCAGTATGGTGGAGA GGCCATATGGAAGAAAGAGTAAAGACAAGATTGCGTCTTACAGCAAAACTTCAAAAATTGACCGAGGTGA TATGGGCAAAGAAATAAGAGAGAAATCATCCATGAAGCGTAAACTGCCTTTTACACTCAGCCCATCGAGA AATGAAGAGCGAGATTCAGACACAGATTCAGACCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTA TATCTTCTTACAGGACATACTCAGAGAAAGAAGGTCCAGAAAAGAAGAAAACAAGAAAAGAAGCTGGAAC TAAGAAGTCCACCCCAGTGAGCATTCTTTTTGGTTACCCACTCTCTGAACGAAAGCAGATGGCGCTCCTA ATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCATCACAAGCAACACCAGCCC AGAAGAAAACTCCCAGTTCTTCATCTCGACAAAAAGATAAAATTAATAAAAGAAATGAACGTGGCGAGAC TCCTTTACACATGGCAGCTATTCGAGGAGATGTGAAACAAGTCAAAGAGTTAATTAGCTTAGGGGCAGAT GTGAACGTGAAAGACTTTGCCGGTTGGACACCTCTGCATGAAGCTTGCAATGTTGGGTATTATGATGTTG CTAAAATACTCATCGCAGCGGGAGCAGACGTTAACACGCAAGGGCTGGACGACGACACGCCCCTCCATGA CTCCGCCAGCAGTGGGCACAGAGATATAGTGAAATTGTTGCTTCGTCATGGCGGGAATCCATTCCAAGCC AATAAGCATGGAGAACGGCCAGTGGATGTGGCAGAGACAGAGGAGTTGGAGTTGCTGCTAAAAAGAGAGG TGCCTTTGTCTGGTGATGACGAAAGTTATACAGATTCAGAAGAGGCGCAGTCTGTAAATCCTTCCAGCGT TGATGAAAACATCGACTCAGAGACAGAGAAGGACTCTTTCGTCTGTGGAAGCCAAAGCCTCCCAAGCAAA GCCCCACTGCCCTCTGCCCTGGACGAGTACGAGTTCAAGGATGACGATGGAGAGGAGATCAGTAAGATGA TCGATGGCAGGCACATTCTCAGGAGAGAGCAACGGAAAGAGAATGAGTCAGAAGCAGAAAGGAGTGGTCT GTTTGCAAAACAGGAAAAACCCTTCTATTCTAAGTCATTTAAAACTAAAAAACAGAAGCCGTCACGGGTT CTGTACTCCAGTACTGAAAGTTCTGATGAAGAAGTTCTTCAGAACAGAAAGATTTCTACTGCATGTTCTG TTGCCGAAACATCACATTCTGATGTGCAAGCCAAAAGAGAGTATGAGTACAAACAGAAAGGCAAAGTTAA GAGAAAATTAAAGAATCAGAATAAAAATAAAGAGAATCAAGAGCTGAAACAAGAAAAGGAGGGGAAAGAA AATAGCAGGATTGCAAACCTGGCAGTTAACACTGCGCTGGATTGTTCAGAGAGAAACAGAGAGGACGGGG CCTTCAGAAGATCGTTTAGCCCCAAAGACGATGCCTCTCTGCACTTACTTCATATGCCCACTGGTAAATC TCCTAAGCATCCTTGTGGATTGAGTGAAAAACAGGCAACACCACTGAAGCAAGACCACATGAAGACGTGC ATATCCCCAGGAAGTTCTGAAACGTCACTGCAGCCTGAGCTCTCTCGGTATGAGAATGCAGAACCTGAGT TCTTGCCGGAAAGCTCAAATGTCAAACCTTATAAGCACAAGGAAAAAAACAAACATCAGAAAGATTTTCA CCTGGAATTTGGTGAAAAGTCAAATGCCAAAATAAAAGATGAAGACCATAGTGCAGCGTTTGAAAACTCA GACTGCACACTGAAGAAGATGGACAAAGAGGGCAAAACATTAAAGAAGCATAAACTAAAGCATAAAGAGA GGGATAAGGAGAAACACAGAAAGGAAGCCGAAGGTGAAAAGGAAAAATATAAAAGCAGGGATAATGCCAA AGAGCCACAGAGGAGCATTGAGTTTGACAGAGAGTTTTGGAAAGAAAACTTCTTTAAAAGTGATGAAACC GAGGATCTCTTCTTAAATATGGAACATGAGTCCCTAACAGAAAAGAAGTCCAAAGTGGAGAAAACCGTGA AAGATGATAGATTAGCCAAGGACAAGCACGCCTCAAAAGAGAGGAACTGTAAAGAAGAACGGGAAAAGAC TAAAAGAGAAAACGAAAAGTCCCTTAAGGAAGAAAGGCTGAAAGATCCGAAAGAAGAAAAAGATGGTGTA TCGGCAGAGAAGGAAGCAGAGGGCAGTTCCATGGCTGTCAGTGCAAACCAGGAAACTTCAGGGCCACACT CTTCAGAACGGGAAGTAGACATTGAAAAGCAAGAGCGGCACTCGAAAGAAAGGGAAAAATCTGACAGACG CTTTCAGACTAAGGAAAAAGAGCTTGACAGGACAGAAAGAAAAGTTTCTGAAAAAGAAAAGAAGCTAAAA CATGAGCATAAGTCAGAAAGAGAGAAGCTGGATTTTAGTGACTGTACTGACAGAGTGAAAGAGAAGGACA GGCTGTACTCACCTCAGACAGAACGGTGCCATAAGGAAGGTGAGAAGATGAGAAACACTGTCAGAAAAGC TGAAGACAGGGAGCGGGCCAGGGAAAAGACAGACAGAAAGCATGACAGAGAGAAACCTGAGCGAGAGAGG TGTTTAGCAGAAAGCAAAGAAAAGTACTTAGAGAAAAGAAAGCAGTCGGACAACAGTGAGTACTCTAAGT CAGAGAAGGTCAGAAATAGAGATAGAGACAGGGAAGGAGAGAAGAAGGAGAAACTCAGAGATAAAGAAAG TGTAAATGTAGCCAACTTGAGACATTTACAGGAGGAGAGAAAGTCAAGTACAGGAGAGGGCAGTAGTAAA ACCCAGCATGAGAAAGCCCTGTCCCTTAAAGAAAGGGTAAGAGATGAGCCTTTGAAAATCCCAGATGGGA AAGAGAAAGATAAAAAGGATATAGATAGATACAAAGAGCGAGACAAGCGTAAGGAGAAAGCCCAGCTAAG CACTTTAATCAGACTAAAATCGGAAACAGAGAAGCTTAAACCTAAATTGTCACCAGCATCAAAAGATGCT CGACCTAAAGAAAAGAGGCTAGTAAATGATGACTTGATGCAGACGAGCTTTGAACGGATGCTCAGCCTTA AAGACCTAGAAATAGAACAGTGGCACAAAAAACATAAGGAAAAAATTAAGCAAAAAGAAAAAGAACGATT ACGGAATCGTTCTTGTTTAGAACTTAAAGTAAAAGATAAAGAAAAAACAAAACATACACCCAGTGAATCC AAAAATAAAGAACTTACCAGGTCAAGAAGTTCAGAGTTAACCGATGTTTATAACAAGGAGAAACAATCTA AAGATGTTGGAAGTAATCGCTCGCAATCTGTTGATAACAAAAGTGTATTGAGTTTAGGAAAGCTACCCTA CGTTTCAGAGAATAGCTCAAGCCGGTCTCCTAGGTCAGAAGGTGAGAAGCTGGGTCTCAGCTCCAGGTCT GTGTCCATGCTCTCTGTTGCTAGTTCAGAAGACTCCTGCCACACAGTCACAACCCCAAGGCCCCTCGTCG AGTATGACTCTGACTTCATGTTAGAGGGCTCAGAATCTCAGTTGTCATTTTCTCAGTCACCTTTTTTGCC GAATGCCAAATCTCCAGCTCTTTATGAAAGAGAGCTGGAGAGTCTCGCTGAACTGCCAGAACGACTTAAA CCACCGTATGCAAACAGACTTCCAGTATGCCATCTCAGGTCATCGTCTGTGGAAGACGTTAAACTGATCA CAAATGAGGTAAGACCTGCTGTAGAAATCAGAAGATGTAGCATGCCCTCAGTCATTTGCGAACCCATAAA ACATTTCCAAGTATCAGAAGACAGCAGTCAAGGCAACTTAGCTGTGCCAAGAGAGACTTCTCTGTCTCCC AGACCTGAAGTGTCTTTAGTACCTGAAAGAGGCCTTTCGCACGTGTCTGACTTACATCCTCGCTGTACAG ACTCCCCAGTGAGGTCTGTAGACAGCAGCTCTGTTTCATCTGACGTACATGTAATCAAGAGTGCTGCTCT TGTGGGTACACTGATTGACACGTCAATGCATTTGGAAACATCCAATCAGGTTGGAGTAATCCAAAGTAAA TCATGGGAGACGCCTGTTGACAAACTGGAGTCTTTAAGCACCAATGACTTTATCTGTCCAGATTCTAGTA CATCTGATCAAGATTCCTCTGCTCAGAGTTTTTATCATTCAGAAAACAAAATGTTAAGAGAACAAAATGC TGATTGCTTACCTGTACACCAGACTGAACTGCCAGGAAACTCGTGTGCTCAGGACCCAGCATCCTTCACC CTGTCTTCACCCCAGCCCTGCTCTTTTACCAACCAGTCACTCTCAGACGCTGAATCTCTTCCTAAACCTG TGTCTTTGTCTTACGGTGCCAGTCAAGAGCCAGGTGTTTTACAACAGAGAAGTGCAGCTCACATGATCAG TTCTGTTTTAGAGAGTGATAATAACTTTACAAAGGAGGTGGAAAGTTCGTTTGTCTTAGCAGACATTCAG AAGACGAATTCTTTTGTACCAGCTTACTCTGAAAGCACTGTGCAGGAAACACTCCCAAACTTTGAGAAGG CCAATACCTTGCTTGTGTTGCCATCAGAAAAGGATTTCAGTGGGAGTGACGCTGCTTCTCAGCTAAACAC ACATTATGCCTTTAGCAAACTTACATACAAGTCTTCCAGCAGCCATGAGATTGAGAAGGGCCCAGATGAT ACTCAAGTCATTTCCCATGAAAAAGAAAACAAACTGCAAAGTTTGGTCTTAACGCCATTGAGTAAGTGTG ATTCTGATTTGTATGAAGTGAATGCAGGGATGCCGAAAGGAAACCTGAATGAACAGGATAACCCAAAGCA CGGTCCTGACAGCGAAAAGGATTCGCTTTCCACTGAGGATGAGGAATCTCAGCAGAGCACTTTATCAAGT GTGGAAAACCATTCACAGCAGTCAGCCCAACCAGAAATACAGAAATATGACCAGATGGTTAAAGGAGAAT TAGAGGAAAATGCTGAGGACGATAAAACTGAAAACCAAACCCCTCAAAGAATGACTAGAAACAAAGCAAG TGCACTGGTCAGTCAAAGCAAGCAGGTTCTTGCTGGCTGTACACTTTTACCAGAGAAAGATAATGACTCC TCCCCTCGGGGGAGAATCAGACTCACCGAAGAAGACGATCCTCAGATCCACCATCCACGGAAAAGGAAAG TGTCACGGGTCCCTCAGCCTGTGCAAGTGAGTCCCTCTCTACTACAAGCAAAGGAGAAGACGCAGCAGTC CCTAGCAGCTCTCGTAGACTCTCTGAAGCTAGACGAGATCGAGCCGTACAGTTCCGAGAGAGCAAATCCA TATTTTGAATATTTGCACATAAGGAAAAAAATTGAAGAAAAACGCAAGTTGCTATGTAGTGTTATTCCTC AAGCTCCTCAGTATTATGATGAATATGTAACGTTTAATGGATCATATCTCCTGGATGGAAACCCCTTAAG CAAGATTTGTATTCCTACAATCACACCGCCACCTTCGCTGTCAGATCCACTTAAAGAGCTCTTTCGGCAA CAGGAAGTAGTAAGGATGAAACTGCGTTTGCAACATAGTATTGAAAGGGAAAAACTTATTGTATCGAATG AACAAGAGGTTCTGCGAGTTCATTACAGAGCTGCAAGAACACTGGCCAATCAAACACTGCCATTCAGTGC TTGCACTGTTTTACTGGATGCCGAAGTGTACAATGTGCCCCTGGACTCACAGTCTGATGACAGCAAAACT TCTGTGAGAGATCGATTTAATGCAAGACAGTTCATGTCCTGGTTGCAAGATGTGGATGATAAATTTGACA AGCTAAAGACCTGTCTGTTAATGAGGCAGCAACATGAGGCTGCAGCTCTAAATGCCGTCCAGAGACTAGA GTGGCAGCTCAAACTCCAGGAACTGGACCCTGCCACCTATAAATCTATCAGTATTTACGAGATCCAGGAG TTCTATGTTCCGCTTGTTGATGTTAATGATGATTTTGAATTGACTCCTATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108238
Insert Size	6144 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108238.1, NP_001101708.1
RefSeq Size	9836 bp
RefSeq ORF	6144 bp
Locus ID	316775

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