Hecw1 (NM_001106117) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1561038
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210105 representing NM_001106117 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTGTACCAGAACAGGTTTTTAGGCCTGGCTGCCATGGCATCTCCATCTAGAAACTCCCAGAGCC GACGCCGGTGCAAAGAGCCCCTCCGGTACAGCTATAACCCAGACCAATTCCACAACATAGACATCAGAAA TGGTGCCCATGATGGCATCACCATCCCTCGCTCTACCAGTGACACTGACCTGGTCACCTCAGACAGCCGC TCTACACTCATGGTCAGCAGTTCCTACTATTCTATAGGGTATTCCCAGGATTTGGTGATCCACTGGGACA TCAAGGAAGAAGTGGATGCCGGAGACTGGATTGGGATGTACCTTATTGGTGAAGTCTCATCTGAAAACTT TCTGGACTATAAGAACCGTGGAGTCAATGGATCTCACAGAGGCCAGATCATTTGGAAGATTGATGCCAGC TCTTACTTTGTGGAATCTGAAACTAAGATCTGCTTCAAATACTACCACGGAGTAAGCGGGGCCCTGAGAG CTACAACCCCCAGTGTCACTGTCAAGAACTCAGCGGCTCCTATTTTTAAAGGTATTGGCTCTGAGGAGAC TGTGCAAAGTCAAGGAAACAGGAGGCTCATCAGCTTCTCTCTCTCAGATTTCCAGGCCATGGGATTGAAG AAAGGGATGTTTTTCAACCCAGACCCTTATCTGAAGATTTCCATTCAACCTGGTAAACATAGCATCTTCC CGGCCCTCCCTCATCATGGACAGGAACGGAGATCCACGATCATAGGCAACACTGTAAACCCCATCTGGCA GGCAGAGCATTTCAGTTTCGTGTCTCTGCCAACTGATGTGCTGGAAATTGAAGTGAAGGACAAGTTTGCC AAGAGTCGTCCCATCATCAAGCGTTTCTTGGGAAAGCTGTCAATGCCAGTTCAGAGGCTACTGGAGAGAC ATGCCATAGGGGATAGGGTGGTCAGCTACACACTTGGCCGCAGGCTTCCAACTGACCATGTGAGTGGACA TCTGCAATTCCGATTTGAGATCACATCCTCCATCCACGCAGACGATGAAGAGATCTCTCTGAATGCTGAG CCTGAGTCAGCGGAAGTGCAGGACAGTGTCATGAATAGTGTGGTGGAAAACAGCAGTGGGGAGCCTCCCG GTGATGCTGCAGAATTCCTCAAGGACTTGAAGCCAGAGGCACCGAGTGAAGAGGATGGAGTGCGCGGCTC AGAAAATGAAAACCGAGAGCATGCCAGGCCAGTCGAGGAAGCTGCGGGTGCTATGGAGACCAGAGATGAT AGCAAGTTCTCTGAAGGACCGGAAGAGGTTGGAGAGCTTCAGGACCCAGAGCAACATGACACCCAGCCCA CTCTGAGCGCAGAAGAAGTGGCAGAGCGACTTCCCCTGGGTGAGGGTTGCCCACCCTCGCTGTTGCCAGA AGAGAGTACAGCTCTAGGCAGCAAGGCAGAAGAGGAAGCAATTCCAGAAAATGGAGCGGGAGAGGAAGAG ATGCAGGAGAAGGGGAAAGATGAGGAGGAAGAAGAGGGTGTTTCTGCCCTGGAGCAAGGAGAGCCTGGAC TTGAGCTGAGGGCCTCCGTGAGAAAAAAAAGCAGGCCATGTTCCTTACCTGTATCCGAGCTTGAAACAGT GATCGCATCTGCCTGTGGAGAGGCTGAGACCCCAAGGACCCACTACATCCGCATCCACACCCTACTGCAC AGCATGCCCTCGGCCCAGAGGGGAAGCACCACAGAGGAGGAGGATGCCATGGAGGAGGAATCCACCCTCA AGGAGTCCTCTGAAAAGGATGGGCTCAGCGAGGTGGACACAATAGCTGCTGACCCACAGTCCATGGAAGA TGGCGAGAACAATGGCGCTACTCTCTGCATGGCATCCTCTGACTGCTCTGGGGGCCATTTCTCCAATTTA TCTAAAAGCATTGGCGCTGGCCAAGATGGCGAAGCTCACCCCAGCACTGGGAGTGAGAGTGACTCCAGCC CCCAGCAGGGAGCAGACCACAGTTGCGAGGGCTGTGATGCCTCATGCTGTAGCCCCTCATGCTATAGCAC ATCGTGCTACAGCAGCTCCTGTTATAGCAGCTCCTGTTACAGCAGCTCCTGCTACAATGGCAACAATCGG TTTGCCAGCCACACACGCTTCTCCTCGGTGGACAGCGCCAAGATCTCTGAGAGCACAGTCTTTTCTTCAC AAGAGGATGAGGAGGAGGAAAACAGTGCATTCGAGTCGGTACCTGACTCGGTGCAAAGCCCAGAGCTAGA TCCCGAGTCTACAAATGGGGCTGGGCCTTGGCAGGATGAACTGGCTGCCCCTGGTGGGAATGCAGCAAGA ACTACTGAAGGTCTGGACTCCCCCATGGCAGGCCCTAGCAATAGGAGAGAAGGTGACTGTCCTATGCTCC ATAATTCCCAGCCAATAAGCCAGCTTCCTTCCTTGAGGCCTGAACATCATCACTACCCAACAATCGATGA GCCTCTTCCACCAAACTGGGAAGCTCGAATCGACAGCCATGGACGGGTCTTTTATGTGGACCATGTAAAT CGCACAACCACCTGGCAGCGACCATCAATGGCGCCTACCCCAGATGGAATGATCAGATCCGGGTCTGTCC ACCAGATGGAGCAGCTTAACAGAAGGTATCAAAACATCCAGAGAACCATTGCAACAGAGAGATCTGAAGA AGATTCTGGCAACCAAAGCTGTGAGCAAATACCAGACGGAGGAGCTGGAGGTGGAGGGAGTGACTCGGAA GCAGAATCTTCCCAATCAAACTTAGATCTGAGGAGAGAAGGGTCACTTTCTCCTGTCAACTCACAGAAGG TCACCTTGCTGCTGCAGTCTCCAGCAGTCAAGTTCATCACCAATCCTGAGTTCTTCACTGTGTTGCATGC CAATTATAGTGCCTACAGGGTTTTTACAAGTAGCACTTGCCTGAAGCATATGATTTTGAAAGTCCGAAGA GATGCTCGCAATTTTGAACGCTACCAGCACAACCGGGACCTGGTGAATTTCATCAACATGTTTGCAGATA CTCGGCTGGAACTGCCCCGGGGTTGGGAGATCAAAACTGATCATCAGGGAAAGTCTTTCTTTGTGGACCA CAACAGTCGGGCTACTACTTTCATTGATCCCCGAATCCCTCTTCAGAATGGCCGCCTTCCCAATCATCTA ACCCACCGACAGCACCTCCAGAGGCTCAGGAGTTACAGTGCTGGTGAGGCCTCAGAAGTCTCTAGAAACA GAGGAGCATCTTTGCTGGCCAGACCAGGACACAGCCTAATAGCTGCTATTCGGAGCCAACATCAACACGA GTCCTTGCCACTGGCATACAATGACAAGATTGTGGCATTTCTTCGACAGCCAAACATTTTTGAAATGCTA CAAGAACGTCAGCCAAGCTTGGCAAGAAACCACACACTCAGGGAGAAAATCCATTATATTCGGACAGAGG GCAATCATGGACTTGAGAAATTGTCCTGTGATGCAGATCTAGTCATTTTGCTAAGTCTCTTTGAAGAAGA GATCATGTCCTATGTCCCCCTGCAATCTGCCTTTCACCCTGGATATAGCTTCTCTCCCCGCTGTTCACCC TGTTCCTCACCCCAGAACTCCCCAGGTTTACAGAGAGCCAGTGCCAGGGCCCCTTCACCGTACCGAAGGG ATTTTGAAGCTAAGCTCCGCAATTTCTACCGAAAACTGGAAGCCAAAGGATTTGGGCAGGGTCCGGGTAA AATTAAGCTCATCATCCGTCGAGACCACTTGTTGGAGGGGACCTTCAATCAGGTGATGGCATACTCTCGG AAGGAGCTCCAGAGAAACAAGCTCTACATCACCTTTGTTGGAGAGGAGGGGCTGGACTACAGTGGCCCTT CTCGAGAGTTCTTCTTCCTGTTGTCTCAGGAGCTCTTCAACCCTTACTATGGACTCTTTGAGTATTCTGC AAATGACACTTACACAGTGCAGATCAGCCCCATGTCGGCGTTTGTAGAAAATTATCTCGAATGGTTTAGA TTCAGCGGCCGTATCCTGGGCCTAGCTCTGATCCACCAGTACCTCTTGGATGCTTTCTTCACCAGACCCT TCTATAAGGGACTCCTGAAGCTGCCATGCGATCTGAGTGACCTGGAATACTTGGATGAGGAATTCCACCA GAGTTTACAGTGGATGAAGGACAACAACATCACAGATATTCTTGACCTCACTTTCACTGTTAACGAAGAA GTTTTTGGACAGGTAACAGAAAGAGAGTTGAAGTCTGGAGGTGCCAACACGCAGGTGACTGAGAAGAACA AGAAGGAGTACATCGAGAGGATGGTGAAGTGGCGTGTAGAGCGTGGTGTGGTACAGCAGACCGAGGCATT GGTCAGGGGTTTCTATGAGGTTGTAGACTCAAGGCTTGTCTCAGTGTTTGACGCGAGGGAGCTGGAGCTG GTAATCGCGGGAACTGCTGAAATCGACCTGAATGACTGGAGGAATAACACTGAGTACCGGGGAGGTTATC ATGATGGCCACCTTGTGATCCGCTGGTTCTGGGCTGCAGTGGAGCGCTTCAATAATGAGCAGAGATTGAG ATTACTTCAGTTTGTGACAGGAACGTCGAGTGTTCCCTATGAAGGCTTTGCAGCTCTGCGGGGGAGCAAT GGGCTTCGAAGGTTCTGCATAGAGAAGTGGGGGAAGATTACATCTCTTCCCAGGGCACATACATGCTTCA ACCGACTGGATCTCCCACCGTATCCTTCATACTCCACATTGTATGAAAAGTTGTTAACAGCAGTTGAAGA AACCAGCACCTTTGGACTTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106117
Insert Size	4785 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106117.1, NP_001099587.1
RefSeq Size	5178 bp
RefSeq ORF	4785 bp
Locus ID	291209

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