Iws1 (NM_001034918) Rat Untagged Clone
CAT＃: RN209659
Iws1 (untagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug)
CNY 9880.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1304762 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN209659 representing NM_001034918
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGAGTACTACAGCGGTGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG ATTCAGGGTCAGATGGTGAGGATGATGTAAACGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCG CCATTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGACCAAGATACATAATGGAACAGACTCTGAG AATGATGAGCCCTCCAATGTTCATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAAGACAGTG ACTCTGAATCCGAGGAACATGCAGAGTCTCCTGCCAGTGATTCTGAAAACGAAGCTGTCCACCAGCAGGG GAGTGACTCTGAAAAGGAAGAGCTTCTTAATGGGCATGCAAGTGACTCTGAAAAGGAAGAGGGTAGAAAG CATGCAGCCAGTGACTCTGAAACCGAGGACACTCTGCAGCCTCAGGGCAGCGAGTCTGACAGTGAAGATC CCCCAAGGCCACAGGCCAGTGATTCAGAAAGCGAGGAGCCTCCCAAACCTCGGATCAGTGATTCAGAAAG CGAGGAGCTCCCCAAACCTCGGATCAGTGACTCAGAAAGTGAGGATCCTCCAAGGCCCCAGGTCAGTGAC TCAGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGTGATTCAGAAAGTGAGGATCCCCCAAGGCCACAGG CTAGTGACTCGGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACATAAAGAAAAGCCAGAGTCAGAGGACAGT GATGGGGAGAATAAGAGGGAAGACTCTGAAGTGCAGAATGAGTCAGACGGCCATGCAGACAGAAAAGGAC TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAGAGGCAAAAGATTGACAGTGATGACGACGGAGAAAA GGAGGGGGATGAGAAAGTAGCAAAGCGGAAGGCTGCCGTGCTTTCTGATAGTGAGGACGAAGACAAAGCA TCAGCAGCAAAGAAGAGTCGAGTTATCTCTGATGCTGATGATTCTGATAGTGATGTTGTATCAGACAAAT CAGGCAAAAGAGAGAAGACGGTAGCATCTGATAGTGAAGAAGAAGTCGGGAAAGAAGAGTCTTCTGTAAA GAAGAGTGAAGAGAAGGATCTGTTTGGGAGTGATAGTGAATCAGGCAATGAAGAAGAAAATCTTATTGCA GATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACAGGATTTAACCAAGAAGATTTGGAGG AAGAGAAAAATGAAACACAGCTAAAAGAAGCAGAAGATTCTGATTCCGACGATAACATAAAGAGAGGAAA GCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAGAGCATGTGTGGCAAACGCAGA CGAAATCGAGATGGGGGCACTTTTATTAGTGACGCAGATGATGTCGTGAGTGCCATGATTGTCAAGATGA ACGAAGCTGCTGAGGAAGACAGGCAATTGAATAATCAAAAAAAGCCTGCTCTGAAAAAATTAACCTTATT GCCTACTGTGGTTATGCACCTTAAAAAGCAGGACCTTAAAGAAACATTTATTGACAGTGGTGTGATGTCT GCCATTAAAGAGTGGCTCTCACCTCTACCAGATAGGAGTTTGCCTGCACTGAAGATTCGGGAGGAATTGT TAAAGATTCTGCAAGAGCTACCTAGTGTGAGCCAAGAGACCCTGAAGCACAGTGGGATTGGCCGAGCAGT GATGTATCTGTATAAACATCCTAAGGAATCAAGGTCCAACAAGGACATGGCAGGGAAACTAATCAATGAG TGGTCTCGGCCCATATTTGGTCTTACCTCAAACTATAAAGGAATGACAAGAGAAGAAAGGGAGCAGAGAG ATCTAGAACAAATGCCACAGCGACGAAGAATGAGCAGCACTGGTGGTCAGACACCACGAAGAGACCTGGA AAAGGTACTAACAGGAGAGGAAAAGGCTCTTAGACCTGGAGATCCTGGCTTCTGTGCCCGTGCGAGAGTC CCAATGCCATCAAACAAGGACTATGTTGTCAGGCCCAAGTGGAATGTAGAAATGGAGTCATCCAGGCCTG GTATTCTTAAAAAGGGTCTAAGTCGATTGGAAAAGCATAAGAGGCGGTTTGCAGAGCAGAAGAGGCTCAG CCAAATGCACCGTGCTGTCAAGTTCAGCATCGAAGGCAACAGAATGCCCCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001034918 |
| Insert Size | 2295 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001034918.1, NP_001030090.1 |
| RefSeq Size | 3010 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 291705 |
| UniProt ID | Q3SWT4 |
| Gene Summary | Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR209659 | Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
CNY 9880.00 |
|
| RR209659L3 | Lenti ORF clone of Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
CNY 11690.00 |
|
| RR209659L4 | Lenti ORF clone of Iws1 (mGFP-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
CNY 12830.00 |
