Kif16b (NM_001107783) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209080 representing NM_001107783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCGGTCAAGGTGGCCGTGAGAGTCCGGCCCATGAATCGCAGAGAAAAGGACTTGGAGGCCAAGT TCATCATTCAAATGGAGAAAAGCAAAACGACAATCACAAACTTAAAGATACCAGAAGGAGGAACTGGGGA TTCAGGCAGAGAACGGACCAAGACCTTCACCTATGACTTCTCTTTTTATTCTGCTGACACAAAGAGTCCA GATTATGTTTCACAAGAAACGGTTTTCAAAACTCTGGGCACAGATGTTGTGAAATCTGCATTTGAAGGTT ATAACGCTTGTGTCTTTGCATATGGGCAAACTGGATCCGGAAAGTCCTACACTATGATGGGAAATTCTGG GGATTCTGGCTTAATACCTCGGATCTGTGAAGCTCTCTTCAGTCGGATCAATGAAACCACTAGATGGGAT GAAGCCTCTTTTAGGACAGAAGTCAGCTACTTAGAAATTTATAATGAACGTGTGAGAGACCTACTTAGGC GGAAGTCATCTAAGACCTTCAATTTAAGAGTCCGTGAGCATCCCAAAGAAGGACCGTATGTTGAGGATTT ATCCAAGCATTTAGTCCAGAATTACAGTGATGTGGAAGAACTTATGGACGCAGGAAATATCAATCGTACC ACTGCAGCGACGGGGATGAATGATGTCAGTAGCAGGTCGCACGCCATCTTCACCATCAAGTTCACTCAGG CAAAGTTCGACGCCGAAATGCCGTGTGAAACTGTGAGCAAGATCCACCTGGTCGACCTTGCAGGAAGTGA GCGGGCAGATGCCACTGGTGCCACTGGGGTCAGACTTAAGGAAGGGGGCAACATAAACAAGTCCCTTGTG ACTCTGGGAAATGTCATTTCTGCCTTAGCTGATTTATCTCAGGATGCAGCCAACCCTCTTGTGAAGAAGA AGCAAGTTTTTGTGCCTTACAGGGATTCAGTTTTGACTTGGCTGTTGAAAGACAGCCTTGGAGGAAACTC TAAAACCATCATGATTGCCACCATTTCACCTGCTGATGTCAATTATGGAGAAACCCTAAGTACCCTTCGC TATGCAAATAGAGCCAAAAACATCATCAACAAGCCTACCATTAATGAGGATGCCAACGTCAAACTCATCC GTGAGCTGCGAGCTGAAATAGCCAGGCTGAAAACCCTGCTCGCTCAAGGGAATCAGATTGCTCTCTTGGA CTCCCCTACAGCTTTAAGTATGGAGGAAAAACTTCACCAGAATGAAGCAAGAGTCCAAGAATTGACCAAG GAATGGACAAACAAGTGGAATGAGACCCAGAATATTTTGAAAGAGCAAACCCTAGCCCTTAGGAAAGAGG GCATTGGTGTGGTTCTGGACTCTGAGCTGCCTCACCTGATTGGCATTGATGATGACCTTCTGAGTACAGG AATCATCTTGTATCACTTGAAGGAAGGTCAGACTTACGTTGGCAGAGAAGATGCTTCAACAGAGCAAGAT ATTGTCCTTCATGGCCTTGACTTGGAAAGTGAGCATTGTGTCTTTGAGAATGCTGGGGGAACTGTGACTC TGATACCCCTGAGTGGGTCCCAGTGCTCTGTGAATGGTGTTCAGATTGTGGATGCCACACAGCTAAATCA AGGTGCTGTGATACTCTTGGGAAGAACCAACATGTTCCGCTTTAACCATCCGAAGGAAGCTGCCAAACTT AGGGAGAAGAGGAAGAGTGGCCTCCTGTCCTCCTTCAGCTTGTCTATGACCGACCTCTCAAAGTCCTGTG AGAACCTGTCCGCAGTCATGTTGTATAACCCTGGACTTGAATTTGAGAGACAACAGCGTGAAGAACTTGA AAAACTAGAAAGTAAAAGGAAACTCATCGAAGAGATGGAGGAAAAGCAGAAGTCGGACAAGGCAGAGCTA GAGCGAATGCAGCAGGAGGTGGAAACCAGGCGCAAGGAAACAGAGATTGTGCAGCGGCAGATCCGCAAGC AGGAGGAAAGCCTCAAACGCCGTAGCTTCCACATTGAAAACAAGCTGAAGGATCTGCTGGCTGAGAAGGA AAGGTTTGAGGAGGAGAGGCTTCGGGAACAGCAGGGACTGGAGCAGCAGAAGAGGCAGGAGGAAGACAGC TTCTTCCGCATCAGAGAAGAGCTTCGCAGGCTCCAGGAGCTCAACAGCCATGAACAAGCCGAGAAGGGCC AGCCACTCCAGGATTTGGACTGGCTGCACAGAGAGCAAGATGCCCAGAGTGCCAAGCTGCAGCTGGAGAA AAGGAGGCTGGAGGAGCAAGAGAAGGAGCAGGTGCTGCGGGTGGCACACCTGGAGGAGCAGCTTCGGAAG AGGCAGGACACTGCTCCTCTGCTGTGTCCTGGAGAGGCCCAGCAGGCCCAAGAGGAAAGGAGGGAACTGG AAGGCATCCGGGGAGCCCTCTTGCAGGCCAAAGAGATCAGAGCAGGAGGGGACCACACCTTCAGGGGTGA ACTGGAAAGGGCTCAGCAGTACTTCTTAGAGTTCAAGAGAAGACAGCTTGTCAAGCTAGCAACCCTGGAG AAAGACCTGGTCCAACAGAAAGACCGCCTAAGCAAAGAAGTCCAGGAAGAGAAAGAGGCCCTGGAGCATG TAAAATGTGAAGCAGGTGAGGAACCGAGCTTGTTGGCAACAGATAATTGCAACATTCTCTGTGGGCCTCA GGATCTAGACAAAATAAAGACTGCAGAGTCCAGGCTGCAGAGCAAAGAGCACCAGCTACAGGACCTTCTG CAAAATCATTTGCCAGCTCTGTTGGAAGAAAAACAGAGAGTGCTTGAAATCCTTGACTGTGGTGTCCTAG GCCTAGATAACATTCTCTACCACGTAGAGAAAGAAATGGAAGAAAAGGAAGAGCAGATCACACAGTACCA GGCTAACGCTAGCCAGCTGCAACAGCTCCAGGCCACAGTCCAGTTCACAGCCAATGTGGCCAGACAGGAA GAGAAGGTGAGAAGAAAGGAAAAGGAAATACTGGAATCCCAGGAGAAGCAGCAAAGAGAGGCACTGGAGC AGGCTGTGGCTAAGCTAGAGCAGAGGCACTCCGCCCTGAAGAGGCGTTCCACCCTGGACCTGGAGATCCA GGAACAGAGGCAGAAACTTGGCTCTCTCCACAGCAGCGAATGGCCAGGGCTACAGGCCAGTCTGGAGGCC AATGGAGAGAGACTCCAGATGGACCCTGCAAGAATAGAACATGAAATCCATGAGCTGAAGCAGAAGATCT GTGAAGTAGATGGTGTTCAGAGACCTCATCATGGGACCTTAGAGGGGAAGGCTGCTCTCTCCAGCTTGCC ACCCAGTGCTGAAAACTCTCACCTGGCCCCACTGATGGATGCCAGGATCAGTGCTTACATTGAGGAAGAA GTCCAGAGGCGCCTTCATGATTTGCACAGGGTGATCAGTGATGCCAGCCATGCACCTGCTGAGCTAAAGA GTAATGAGGAACTTCACAATGGCGCCACTCAACGTAAGCTAAAGTATGAGCGGATGTATTCTCGCTCTTT GGGAGCAAATCCAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCCCGCTATGTCCTGTGTGGTCAA GGCAAGGATGAACACTTCGAGTTTGAAGTTAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCAGGC GTTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAGCTCGCTGCTCTTGAATT CCCACCAAAGAAGCTGTTTGGAAACAAAGACGAGCGGGTAGTTGCTGAGCGGCGGACACACTTAGAGAAA TACCTCAGGGAGTTTTTCAGTGTGATGCTCCAGTCCGAAACATCTCCCCTTCACATCAACAAAGTGGGGC TGACCCTGTCCAAGCACACCATTTGTGAATTCTCACCATTCTTCAAGAAAGGAGTCTTTGACTACAGCAG CCATGGGACAGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107783
Insert Size	3936 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107783.2, NP_001101253.2
RefSeq Size	5405 bp
RefSeq ORF	3936 bp
Locus ID	311478

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