Sptbn2 (NM_019167) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Spnb3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209033 representing NM_019167 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCACCCTGTCACCCACTGACTTCGACAGCTTGGAGATCCAGGGCCAGTACAGTGACATCAACA ACCGCTGGGACCTGCCCGACTCAGATTGGGACAATGACAGCAGTTCAGCCCGCCTCTTTGAGAGGTCCAG AATTAAGGCCCTGGCAGATGAGCGAGAAGCCGTGCAGAAGAAAACCTTCACCAAGTGGGTGAACTCCCAC CTGGCCCGGGTGACATGCCGGGTGGGAGACCTGTACAGCGACCTGCGGGACGGGCGCAACCTCCTGAGGC TCCTGGAGGTGCTCTCGGGAGAGACCCTGCCAAAACCCACCAAGGGCCGGATGCGGATTCACTGCCTGGA GAATGTCGACAAAGCACTGCAGTTCCTGAAGGAGCAGAAGGTGCACCTGGAAAACATGGGCTCCCACGAC ATTGTGGATGGGAACCACCGTCTGACCCTTGGGCTAGTGTGGACCATCATCCTCCGATTTCAGATCCAAG ACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGATGCCCTGCTGCTGTGGTGCCA GATGAAGACTGCAGGGTATCCCAATGTCAATGTGCACAACTTTACCACCAGTTGGAGAGATGGGCTGGCC TTTAATGCCATTGTGCACAAACACCGGCCAGACCTGTTGGATTTTGAGTCCCTGAAGAAGTGTAACGCAC ACTACAATCTGCAGAATGCTTTCAATCTGGCTGAAAAGGAACTTGGCCTGACGAAGCTCCTGGATCCTGA AGATGTGAACGTAGACCAACCCGATGAGAAGTCCATCATCACCTACGTGGCCACTTACTACCACTACTTC TCGAAGATGAAGGCCCTGGCTGTGGAAGGCAAAAGGATTGGCAAGGTCCTGGACCATGCCATGGAGGCAG AACACCTGGTAGAGAAATATGAGTCCCTGGCCTCTGAACTGCTGCAGTGGATCGAGCAAACGATTGTGAC CCTCAATGACCGACAGCTGGCCAACTCCCTGAGTGGCGTCCAGAACCAGCTGCAGTCTTTCAATTCCTAC CGCACGGTGGAGAAGCCACCCAAGTTCACAGAGAAAGGGAACTTGGAGGTGTTGCTCTTCACCATCCAGA GTAAGCTGCGGGCCAACAACCAGAAAGTCTACACACCACGCGAAGGCCGGCTCATCTCGGACATCAACAA GGCCTGGGAGCGGCTAGAGAAAGCCGAACATGAGCGAGAGCTGGCCCTGCGCACGGAGCTGATCCGCCAG GAGAAGCTGGAGCAACTGGCTGCTCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGGCTCAGTGAGA ACCAGCGCCTCGTCTCCCAGGACAACTTTGGCCTGGAGCTGGCAGCAGTGGAGGCAGCAGTGCGGAAGCA TGAAGCCATTGAGACAGACATTGTGGCCTACAGCGGCCGGGTGCAAGCGGTGGACGCCGTAGCCGCAGAA CTGGCCGCTGAGCACTACCATGACATTAAGCGCATTGCGGCGCGGCAGAACAACGTGGCCCGGCTCTGGG ACTTCTTACGAGAGATGGTGGCCGCCCGCCGTGAGCGGCTCCTTCTCAACCTGGAGCTGCAGAAGGTGTT TCAGGACCTGCTCTACCTCATGGACTGGATGGCAGAGATGAAGGGCCGGCTGCAGTCTCAGGACCTAGGC AAGCATCTGGCTGGAGTGGAAGATCTACTGCAACTACACGAACTGGTGGAGGCGGACATTGCAGTTCAGG CTGAGAGGGTGCGAGCGGTCAGCGCCTCTGCCCTGCGCTTCTGCGACCCCGGGAAAGAGTATAGACCATG TGACCCACAGCTAGTGTCAGAGAGGGTAGCCACTCTGGAGCAGAGCTATGAGGCCCTGTGCGAATTGGCA GCAACTCGAAGGGCCCGACTGGAAGAGTCCCGTCGTCTCTGGAGGTTCCTCTGGGAAGTGGGTGAGGCCG AGGCCTGGGTTCGGGAGCAGCAGCACCTCCTGGCCTCAGCTGAGACAGGCCGGGACCTGACTGGTGTCCT CCGCCTGCTCAATAAGCACACAGCCCTACGGGGTGAGATGAGTGGCCGCCTGGGGCCCCTGAAGCTCACC CTGGAACAAGGTCAGCAGTTAGTTGCCGAGGGCCACCCTGGAGCTAACCAAGCCTCAACCCGTGCAGCAG AGCTCCAGGCCCAGTGGGAGCGACTAGAAGCCCTGGCCGAGGAGCGAGCCCAGCGGCTAGCACAGGCTGC CAGCCTCTACCAGTTCCAGGCAGATGCCAATGACATGGAGGCTTGGTTGGTGGACGCACTACGCCTGGTA TCTAGCCCTGAGGTAGGGCACGATGAGTTCTCCACGCAGGCCCTGGCCAGGCAGCACAGGGCCCTTGAGG AGGAGATCCGAGCCCACCGGCCTACACTGGATGCCTTGAGGGAGCAGGCTGCAGCCCTGCCACCTGCACT GAGCCACACACCTGAGGTACAGGGCAGGGTGCCCACTCTGGAGCAGCACTATGAGGAGCTGCAGGCCCGG GCAGGTGAGCGTGCACGAGCCCTGGAAGCAGCCCTGGCGTTCTATACCATGCTCAGCGAGGCCGGGGCTT GTGGGCTCTGGGTAGAGGAGAAGGAGCAGTGGCTCAACGGGCTGGCCCTACCTGAGCGCCTGGAGGACCT GGAGGTGGTCCAACAGAGGTTTGAGACCTTAGAGCCCGAAATGAACGCCCTGGCTGCACGGGTTACTGCT GTCAGTGACATAGCTGAGCAGTTGCTGAAGGCCAGTCCACCAGGCAAGGACCGCATCATTGGCACCCAGG AGCAGCTCAACCAAAGGTGGCAGCAGTTCAGGTCCCTGGCAGATGGCAAAAAGGCAGCTCTGACATCAGC CCTGAGCATCCAGAATTACCACCTAGAGTGCACAGAGACCCAGGCCTGGATGAGAGAAAAGACCAAGGTC ATTGAGTCTACCCAGGACCTAGGCAATGATCTAGCTGGTGTGCTGGCCCTGCAGCGGAAGCTGGCAGGCA CTGAGAGAGATCTGGAAGCCATCTCTGCCCGGGTGGGTGAGCTGACCCAAGAGGCAAATGCTTTGGCTGC TGGGCACCCAGCCCAAGCCCCTGCCATCAACACACGGCTTGGAGAGGTTCAAACTGGATGGGAGGATCTT CGGGCAACCATGAGGCGGAGAGAAGAGTCCCTGGGTGAGGCTCGACGGCTGCAAGATTTCCTGCGCAGCT TGGATGACTTCCAGGCCTGGCTAGGCCGCACACAGACTGCTGTAGCCTCTGAGGAAGGACCAGCCACCCT TCCAGAGGCAGAAGCCCTCTTAGCCCAGCATGCAGCTCTGCGGGGAGAGGTGGAGAGAGCCCAGAGCGAG TACAGCCGCCTCAGGACCTTGGGCGAGGAGGTGACCAGAGACCAGGCTGATCCCCAATGCCTCTTCCTCA GACAGAGGCTGGAAGCCCTTGGAACCGGCTGGGAGGAGCTGGGTCGCATGTGGGAGAGCCGGCAAGGCCG CTTGGCCCAGGCCCATGGCTTCCAGGGGTTTTTGAGAGATGCTCGCCAGGCTGAGGGAGTTCTCAGCAGC CAGGAATATGTTCTATCTCACACGGAGATGCCAGGGACACTGCAGGCGGCGGATGCAGCCATTAAAAAGC TGGAAGACTTCATGAGCACCATGGACGCCAATGGAGAGCGCATCCGTGGACTCCTGGAGGCTGGCCGTCA GCTGGTGTCCAAGGGCAATATCCATGCTGAGAAGATCCAAGAGAAGGCAGACTCCATCGAGAAGAGGCAC AGAAAGAACCAGGAGGCCGTGCAGCAGCTTCTAGGCCGCCTTCGGGACAACCGAGAGCAGCAGCACTTCT TGCAAGACTGTCAGGAGCTGAAACTCTGGATTGACGAGAAGATGCTGACAGCTCAGGATGTGTCCTATGA TGAGGCACGCAACCTGCACACCAAGTGGCAAAAACACCAGGCATTCATGGCCGAGCTGGCAGCCAACAAG GACTGGCTGGACAAAGTGGACAAGGAAGGGCGGGAGCTGACTCTTGAAAAGCCAGAACTCAAAGTCCTAG TGTCAGAGAAGCTGGAGGACCTGCACAGGCGCTGGGATGAACTGGAGACTACCACCCAAGCCAAGGCCCG CAGTCTTTTTGATGCTAACCGGGCAGAGCTATTTGCCCAAAGCTGTTCTGCCCTGGAGAGCTGGCTGGAG AGCCTGCAGGCCCAGCTGCACTCAGATGACTATGGCAAGGACCTCACCAGTGTCAACATTCTGCTAAAGA AGCAACAGATGCTGGAACGAGAGATGGCTGTGAGAGAGAAGGAGGTAGAGGCTATCCAGGCCCAGGCAAA AGCCCTGGCCCAGGAAGACCAAAGTGCAGGAGAGGTGGAAAGGACCTCCAGAGCTGTGGAGGAGAAGTTC AGGGCCTTGTGTCAGCCCATGAAGGACCGCTGCCGGCGCCTGCAAGCCTCCCGAGAGCAGCACCAGTTCC ACCGGGATGTGGAGGATGAGATACTGTGGGTGACCGAGCGGCTTCCCATGGCCAGCTCTCTGGAGCATGG CAAGGACTTGCCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACTCTGCAGAAGGAGATCCAGGGCCAT GAGCCCCGGATTGCAGACCTCAAAGAGAGACAGCGCACTCTGGGAACAGCAGCAGCGGGTCCAGAGCTGG CTGAGCTCCAGGAAATGTGGAAACGCCTGAGCCATGAGCTGGAGCTTCGGGGTAAACGACTGGAGGAGGC CCTTCGAGCCCAGCAATTCTATCGTGACGCTGCAGAGGCCGAGGCTTGGATGGGGGAGCAGGAGTTACAT ATGATGGGCCAGGAAAAGGCCAAGGATGAGCTGAGCGCCCAGGCAGAAGTGAAGAAGCATCAGGTACTAG AACAAGCCCTTGCTGACTATGCCCAGACCATCAAACAACTAGCAGCCAGCAGTCAAGATATGATTGACCA TGAACATCCAGAGAGCACAAGGTTAACAATACGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTAAAG GAGCTGGCAGGAGAGCGGCGTGAGCGTCTGCAGGAGCACCTCAGGCTGTGCCAGCTCCGCAGAGAGCTGG ATGACCTGGAGCAGTGGATACAGGAGCGAGAAGTCGTGGCAGCCTCCCATGAACTGGGCCAGGACTATGA GCATGTGACTATGCTTCGGGACAAATTCCGAGAGTTCTCCAGGGACACCAGCACCATTGGCCAAGAGCGT GTAGACAGTGCCAATGCCCTGGCCAATGGGCTCATTGCTGGGGGCCATGCTGCACGGGCCACCGTGGCCG AGTGGAAGGACAGTCTCAATGAGGCCTGGGCTGACCTGCTGGAGCTGCTGGACACAAGAGGTCAGGTGCT GGCTGCTGCTTATGAGCTGCAGCGCTTCCTGCATGGGGCCCGCCAAGCCCTGGCACGGGTGCAGCACAAG CAGCAGCAGCTTCCAGATGGGACGGGCCGCGACCTCAATGCTGCTGAGGCCCTGCAGCGCCGGCACTGCG CCTATGAGCACGACATCCAAGCCCTCAGCACTCAGGTCCAGCAGGTTCAGGACGATGGCCTCAGGCTACA AAAGGCCTATGCTGGAGACAAGGCTGAGGAAATTGGCCGTCACATGCAGGCAGTGGCTGAGGCGTGGGCC CAGCTCCAGGGAAGTTCTGCTGCCCGTCGCCAGCTGTTACTGGATACCACAGACAAATTCCGATTCTTCA AGGCTGTCCGGGAGTTGATGCTGTGGATGGATGGGATTAACCTGCAGATGGATGCCCAGGAGAGGCCCCG GGATGTGTCCTCTGCAGATTTAGTCATCAAAAACCAACAAGGCATCAAAGCAGAGATAGAGGCAAGAGCT GACAGGTTCTCCGCCTGCATTGACATGGGGCAAGAGCTGCTGGCCCGGAACCACTATGCCGCTGAGGAGA TCTCAGAGAAGCTGTCTCAGCTACAGTCCCGGCGCCAGGAGACAGCTGAAAAGTGGCAGGAGAAGATGGA CTGGCTACAGCTTGTTTTGGAGGTGCTTGTGTTTGGGAGAGATGCAGGCATGGCAGAGGCCTGGCTATGC AGTCAGGAGCCATTGGTGCGAAGTGCAGAACTGGGTTGCACTGTGGATGAAGTAGAGAGCCTCATCAAGC GGCATGAAGCCTTCCAGAAGTCAGCAGTGGCCTGGGAGGAGCGTTTCAGTGCCCTGGAGAAGCTCACTGC GCTGGAAGAGCGGGAGAATGAGCAGAAAAGGAAGAGGGAGGAGGAGGAACGAAGGAAACAGCCCCCTACT TCAGAGCCCATGGCTAGTCAACCGGAAGGGAGTCTGGTAGATGGCCAGAGAGTTCTTGACACTGCCTGGG ATGGGACCCAGTCAAAATTGCCACCATCCACACAAGCACCCAGCATTAATGGGGTCTGCACGGACACGGA GTCCTCACAGCCCCTGTTGGAACAGCAAAGACTTGAACAGAGCAATGTCCCAGAAGGGCCTGGATCTGGC ACAGGAGACGAGTCCAGCGGGCCCCGGGGAGAGAGGCAGACCCTGCCCCGGGGCCCTGCTCCGTCTCCAA TGCCCCAGAGCAGATCGTCTGAGTCAGCTCATGTTGCCACCCTGCCCGCACGAGGTGCTGAGCTCTCTGC TCAGGAACAGATGGAAGGGACGCTGTGCCGCAAACAGGAGATGGAAGCCTTCAATAAGAAAGCTGCCAAC AGGTCCTGGCAGAATGTGTACTGTGTACTTCGGCGTGGAAGCCTCGGCTTTTACAAGGATGCCAGGGCAG CTAGTGCAGGAGTGCCATACCATGGAGAAGTGCCTGTCAGTCTGGCCAGGGCCCAGGGCAGTGTGGCCTT TGATTATCGGAAACGCAAACATGTCTTCAAGCTGGGCTTGCAGGATGGGAAAGAGTATCTATTCCAGGCC AAGGATGAGGCAGAGATGAGCTCATGGCTGAGAGTGGTGAATGCAGCCATTGCCACTGCGTCCTCGGCCT CTGGAGAGCCAGAAGAGCCAGTGGTGCCCAGTGCCAGCCGGGGTCTGACCAGGGCCATGACCATGCCCCC AGTGTCACAGCCTGAGGGCTCCATCGTGCTTCGCAGCAAGGATGGCAGAGAAAGAGAGCGAGAAAAACGA TTCAGCTTCTTTAAGAAGAACAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019167
Insert Size	7167 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019167.2, NP_062040.2
RefSeq Size	8186 bp
RefSeq ORF	7167 bp
Locus ID	29211
Gene Summary	may modulate the structural integrity of the Golgi apparatus; binds preferentially to Arp1 rather than actin to mediate the direct interaction of dynein and dynactin with vesicles [RGD, Feb 2006]

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