Cyp27a1 (NM_178847) Rat Untagged Clone
CAT＃: RN207658
Cyp27a1 (untagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 5960.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Cyp27; P450C27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207658 representing NM_178847
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGTTGAGCCGCATGAGACTGAGATGGGCGCTTCTGGACACTCGTGTGATGGGCCATGGCCTCT GCCCACAAGGGGCCAGAGCCAAGGCCGCGATCCCTGCAGCCCTCCGGGATCACGAGAGCACGGAGGGTCC AGGAACAGGTCAAGACCGACCGCGCCTGCGGAGTCTGGCGGAGCTTCCGGGACCCGGAACGCTACGCTTT TTATTCCAGCTATTTCTACGAGGCTATGTGCTGCACTTGCACGAGCTCCAGGCGCTGAACAAGGCCAAGT ACGGCCCAATGTGGACAACCACCTTTGGGACTCGCACCAATGTGAATCTGGCTAGCGCCCCGCTCTTGGA GCAAGTGATGAGACAGGAGGGCAAGTACCCCATAAGAGACAGCATGGAGCAGTGGAAGGAGCACCGAGAC CACAAAGGCCTCTCCTATGGGATCTTCATCACACAAGGACAGCAGTGGTACCATCTGCGTCATAGTTTGA ATCAGCGGATGCTGAAGCCTGCTGAGGCAGCCCTCTACACAGATGCCTTAAACGAGGTCATCAGTGACTT TATTGCCCGGCTGGACCAGGTGCGGACAGAGAGTGCATCAGGGGATCAGGTGCCAGATGTGGCACATCTT CTCTACCACCTTGCCTTGGAAGCCATCTGCTATATCCTGTTTGAGAAAAGGGTTGGCTGCCTGGAGCCCT CCATCCCTGAGGACACCGCCACCTTCATCAGATCTGTTGGACTCATGTTCAAGAACTCAGTCTATGTCAC TTTCCTTCCCAAGTGGTCTCGGCCTCTGCTGCCCTTTTGGAAGCGATACATGAATAACTGGGATAACATT TTCTCCTTCGGGGAGAAGATGATTCATCAAAAAGTCCAGGAGATAGAAGCCCAGCTACAGGCGGCTGGGC CAGATGGGGTCCAGGTATCTGGCTACCTGCACTTCCTGCTGACTAAGGAATTGCTCAGTCCTCAAGAGAC TGTCGGCACCTTTCCTGAGCTGATCTTGGCTGGGGTAGACACGACATCCAATACACTGACCTGGGCCCTG TATCACCTTTCAAAGAACCCAGAGATCCAGGAAGCCTTGCACAAGGAAGTGACTGGTGTGGTACCCTTCG GGAAGGTGCCCCAGAACAAGGACTTTGCCCACATGCCCCTGCTAAAAGCTGTGATTAAGGAGACCCTGCG CCTCTACCCTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTCCTC TTCCCTAAGAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGAGATCCCAGTGTCTTTCCTGAGC CCGAGAGCTTCCAGCCTCACCGATGGCTGAGGAAGAGAGAGGACGATAACTCCGGGATCCAACACCCATT TGGCTCTGTGCCCTTTGGCTATGGGGTTCGGTCCTGCCTGGGTCGCAGGATTGCAGAACTGGAGATGCAA CTCCTGCTGTCAAGGCTGATACAAAAGTATGAGGTGGTCCTGTCTCCCGGGATGGGAGAAGTGAAGTCTG TGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTGAGCCTACGCTTTCTGCAGAGACAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_178847 |
| Insert Size | 1602 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178847.2, NP_849178.2 |
| RefSeq Size | 2150 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 301517 |
| UniProt ID | P17178 |
| Gene Summary | mitochondrial enzyme with both vitamin D3 25-hydroxylase and cholesterol 26-hydroxylase activities [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207658 | Cyp27a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6840.00 |
|
| RR207658L3 | Lenti ORF clone of Cyp27a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 8740.00 |
|
| RR207658L4 | Lenti ORF clone of Cyp27a1 (mGFP-tagged ORF) - Rat cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9600.00 |
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