Smg6 (NM_001105808) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	LRRGT00040; RGD1309609
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207546 representing NM_001105808 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTGCTCCCAGTGCGGCCACCGTCGTTTGGGGAGGGCAGGCAGGAGGGGGCGGGGAAAAGGGTGG GAGTGGGTGAGCGCGCGCCGCCCGTCCGTCGTGGTTTCCTGGCTGCGCGCGGCGGTGGTGGAGTCGCCGC AGCTGTAGCAGCCGCCGCGAAGATGGCGGAGGGGTTGGAGCGTGTGCGGATCTCCGCGTCGGAACTGCGA GGGATCCTGGCCACTCTGGCTCCGCAGGCCGGGAGCAGAGAAAACATGAAGGAACTAAAGGAACCCAGGC AGCGCAAAGATAACAGGCGCCCAGATTTGGAAATCTACAAGCCTGGGCTTTCTCGACTGAGAAACAGACC TAAAACCAAGGAGGCTTCTGGGAATGAGGAATTCAAAGATGAGATTGTGAATGACAGAGATGCTTCTGCT GTTGGGAATGATACACAACTTATTAAAGTTTGCAAGGAATTGGACAGCCAACAGCAAAATGGCCCTATAG ATGCAGAAAATAGTCAGGCACAGGAGACTTTCTCAAAGACTGTTGGACTAGAGGACCGAAGTCTAAAAAT CATCAAGAGAATCAAGAAACCAGACCTGCAGATCTACCAGCCTGGAAGGCGGTTGCAGACTGTTACCAAA GAGTCAGCGGGTAGGGCAGATGAAGAAGAAATCCTCAACCAAGTGGAGCAGTTGACGATGGAAGGGGATG AGTGTAGGGGAGAAGCTATAAAAGAAGAAGTTATCAACAAACCGGATAAAACCGAGATAGAAAAGAGCCA AAGTAATGACCGTGTAAGAACTGCCAAGGGAGAAAAGGGGAAAAAGATTGATAAGGGGGAGGGGTCAAAA AAGGTGACTGATGACTCTGTCCAAGGAAAGCCTGGCTCTGTAAAGAGGTACTCACGATCAGACAAGAGAA GGAACCGCTATCGCACTTGCAGTACCAGCTCAGCTGGTAGCAACAACAGTGCAGAGGGAGCTGGCCTGAC TGATAATGGATGTCGCCGGCGGCGCCAGGACCGGACCAAAGAGAGGCCACGACTGAAGAAACAGGTATCT ATGTCCTCAACTGATTCCTTAGATGAGGACAGAGTTACTGAGCCTGATGGATTAGGGTCCAGGAGGAGCT CAGAAAGGAAGAAGCATTTAGACAGAAATTGGTCTGGCCGAGGTGAGGGTGAACAGAAAAGCAATGGTAA AGAAAACCGAGGCGCCCTTCGTGTCACTTTTGATGCAGAAGCCATGAGTAAAGACTCCCCTGTGGTAAGG TCAGTCAAGGAGAATGTGGATAGAATGAATTCTGACAAAGGCTCAAGCAGTGGGGGTAAGGGCTCTGAGA AGCAGGAGTTCAGAAACCCGAAACAAGAACTTCGAGATCGTGGTCGTGGTATTCTGATTCTGCCTGCCCA CACTGCCCTATCTGTCAGTTCATCAGGTTCTCCGGAGTCTACACCTTTGGGACCTAGACTTTTATTTGGA TCTGGTAGTAAGGGATCTCGGAGTTGGGGCCGTGGAGGCACAACCCGCCGACTATGGGACCCAAACAATC CTGATCAGAAGCCTGCTCTGAAAAGCCAGACACCTCAGCTACATTTCTTGGACACAGATGATGAAATTAG CCCTACATCCTGGGGTGATTCACGTCAGGCCCAAGCATCTTTCTATAAATTTCAAAACTCTGACAACCCC TATTATTATCCTCGGACACCAGGCCCTGCCTCTCAGTATCCCTATACAGGCTATAGTCCTCTGCAATACC CAGTGGGCCCTACAAATGGTATGTATCCAGGGCCTTACTACCCAGGCTATCCTGCCCCCTCAGGACAGTA TGTATGTAGCCCTCTCCCTGCTAGTACCATGAGTCCTGAGGAAATAGAACAGCACGTGCGGAACATGCAG CAACAGGAGCTCCATAGGCTTCTCCGAGTGGCTGACAACCAGGAACTGCAGCTCAGCAACCTGCTTTCCA GGGACCGCATCAGCACTGAGGGCATGGAGAAGATGGCTCAGCTAAGAACGGAACTCCTGCAGCTGTACGA GCGCTGTATTCTATTGGATATTGAATTCTCTGACAGTCAGAATGTAGATCAGATCCTGTGGAAGAATGCT TTCTATCAGGTGATTGAGAAATTCAGGCAGCTTCTCAAGGATCCAAATATTGAGAACCCAGAACAGATTC GGAACAGACTCTTGGAACTCTTAGATGAGGGCAGTGACTTCTTTGATAGTTTGCTTCAGAAACTACAGGT TACTTACAAGTTCAAACTGGAGGACTACATGGATGGGCTTGCCATTCGAAGCAAACCATTACGAAAAACA GTGAAATATGCCTTGATCAGTGCTCAGCGCAGTATGATATGTCAAGGAGATATCTCTAGGTATCGGGAGC AAGCCAATGACACAGCAAACTATGGGAAGGCTCGCAGTTGGTATCTGAAGGCCCAGCATATTGCTCCCAA GAATGGGCGCCCCTATAACCAGTTGGCTTTACTGGCAGTGTATACGAGGAGGAAGCTTGATGCTGTGTAT TACTATATGCGCAGTTTAGCAGCCAGCAACCCTATCCTGACTGCCAAGGAGAGTCTCATGAGTTTATTTG AAGAGACTAAGCGAAAAGCCGAACAGATGGAAAAGAAGCAGCATGAGGAGTTTGATCTGAGTCCTGATCA GTGGCGTAAAGGAAAGAAGTCTACTTTCCGGCATGTTGGGGATGACACTACTCGCCTGGAGATCTGGATT CATCCATCACATTCTCGGTCCTCCCAGGGCACTGAGTCTGGGAAGGATTCTGAGCAGGAGAATGGTCTGG GCAGCCTGAGCCCCAGTGATCTGAACAAAAGGTTCATCCTCAGTTTTCTCCATGCCCATGGGAAGCTGTT TACCCGGATTGGGATGGAGACATTCCCTGCAGTGGCTGAGAAGGTCCTTAAGGAGTTCCAAGTATTGCTG CAGCACAGCCCATCTCCTATTGGAAGCACTCGTATGCTGCAGCTCATGACCATCAACATGTTTGCTGTAC ATAATTCCCAGTTGAAAGACTGCTTCTCAGAGGAGTGTCGTTCTGTGATCCAGGAACAAGCTGCATCCTT GGGCTTGGCTATGTTTTCTCTACTGGTCCAGCGCTGCACATGCTTACTTAAGGACTCTGCCAAAGCTCAG CTGTCCTCTCCTGAGGACCAAGAGGACCAAGATGACATCAAGGTATCTTCCTTCGTCCCGGACCTAAAGG AGCTGCTCCCCAGTGTGAAAGTCTGGTCAGATTGGATGCTTGGCTACCCAGACACCTGGAATCCTCCGCC CACATCTTTGGATCTGCCCTTGCAGGTTGCTGTGGATGTATGGTCAACACTTGCTGATTTCTGTAACATA CTGACTGCAGTGAATCAGTCTGAGGTGCCACTGTACAAGGACCCAGATGATGACCTCACCCTGCTTATCC TGGAAGAGGATCGGCTTCTCTCTGGCTTTGTCCCCTTGCTGGCTGCCCCTCAGGACCCCTGCTACGTGGA GAAAACCTCGGATAAGGTTATTGCAGCTGACTGCAAAAGGGTCACAGTGCTGAAGTATTTTCTGGAAGCC CTTTGTGGACAAGAAGAGCCTCTGCTGGCATTCAAGGGTGGAAAATATGTGTCAGTGGCACCCGTCCCAG ACACCATGGGAAAGGAAATGGGAATCCAAGAGGGAAAACAACTGGAAGATGAGGAAGAAGATGTGGTGAT TGAAGACTTTGAGGAAGATTCAGAGGCTGAAGGTAGTGGGGGTGAGGATGACATCAGGGAACTTCGGGCC AAGAAGCTGGCTCTAGCAAGGAAAATAGCTGAACAGCAGCGTCGCCAGGAAAAGATCCAGGCTGTTTTGG AAGACCAGAGTCAGATGAGACAGATGGAGCTGGAGATCAGACCCTTGTTCCTCGTACCAGATACCAACGG CTTCATTGACCACCTGGCCAGTCTAGCTCGGCTGCTGGAGAGCAGGAAGTACATCCTGGTGGTGCCCCTC ATCGTGATCAATGAGCTGGATGGCCTCGCCAAAGGGCAGGAGACAGACCACCGGGCTGGGGGCTATGCCC GTGTGGTGCAAGAAAAGGCCCGAAAATCCATCGAGTTCCTTGAACGGAGATTTGAGAGTCGGGACTCCTG CCTGCGGGCCTTGACCAGCCGTGGCAATGAACTTGAATCTATCGCCTTCCGAAGTGAGGACATTACTGGA CAGCTGGGCAACAACGATGACCTGATCCTTTCCTGCTGCCTGCACTACTGCAAAGACAAAGCTAAGGACT ACATGCCCACCAGCAAAGAGGAGCCCATCCGCTTGCTACGGGAAGTGGTGTTGCTGACGGATGATCGGAA CCTTCGTGTAAAGGCACTAACAAGGAATGTGCCTGTGAGGGACATCCCAGCCTTCCTTACGTGGGCCCAG GTGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105808
Insert Size	4419 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105808.1, NP_001099278.1
RefSeq Size	6337 bp
RefSeq ORF	4419 bp
Locus ID	287522

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