Nup210 (NM_053322) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pom210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207371 representing NM_053322 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGCCTCGCTGATACAGCCGGGGCTCTGGGCGCTGCTGTTGCTGCAGGCAGTGGGCCCCGCCG TGGCCGCCAAGCTTAACATTCCCAAGGTGCTGCTGCCCTTTACGCGAGCCACGCGCGTGAACTTCACTCT GGAAGCTTCTGAGGGCTGCTACCGCTGGTCATCCACACGGCCAGAGGTAGCCAGCATAGAGCCATTGGGC TCTAGTGAACAGCAGTGCTCCCAGAAGGCAGTGGTGCAGGCCCGCCTGACCCAGCCAGCCCGCCTGACCA GCATCATCTTTGCAGAAGACATCACTACAGGCCAGGTGCTGCGCTGCGACGCCATTGTTGATCTCATCCA CGGCATTCAGATTGTCTCTACCACCCGTGAGCTCTATCTTGAGGACTCTCCGCTGGAGCTGAAGATCCAG GCTCTGGACTCAGAAGGGAATACCTTCAGTACCCTGGCAGGCCTGGTCTTTGACTGGACAATTGTGAAGG ATACAGAGGCCAATGGATTCTCAGACTCCCACAATGCACTACGAATCCTCACCTTCTTGGAATCTACATA TATCCCGCCTTCCTACATCTCAGAGATGGAGAAGGCAGCCAAACAAGGCGACACCATCCTGGTGTCTGGG ATGAAGACTGGAAGCTCCAAGCTCAAGGCTAGAATTCAGGAGGCTGTCTACAAGAATGTTCGCCCTGCGG AAGTCAGGCTGCTGATCTTGGAGAACATCCTTCTGAATCCAGCCTATGATGTTTACCTACTGGTGGGGAC GTCCATTCACTACAAGGTTCAGAAGATCAGGCAGGGCAAGATTACAGAACTCTCAATGCCTTCCGATCAG TACGAGCTGCAGCTCCAGAACAGCATCCCAGATCCCCAGGGAGACCCTGCCCGTCCTGTGGCCGTCCTGA CCCAGGACACATCAAGAGTCACTGCCATGCAGATGGGACAGAGCAACCTGGTCCTGGGCCACAGGAGCAT CCGTATGCAAGGAGCTTCTAGGCTGCCCAACAGCACCATCTATGTGGTTGAAGCCGGGTACCTAGGCTTC ACAGTTCACCCTGGTGACAGGTGGGTGCTTGAGACTGGACATCTGTATGCAGTTACCATCGAAGTATTTG ATAGATCCAGCAACAAGGTCTACCCGTCTGACAACATCCGAATTGAAGCTGTGTTTCCTGCTGAGTTTTT TGAGGTTCTGTCATCTTCCCAGAATGGGTCTTATCATCATGTCAGGGCTATCCAGAGTGGCCAGACGACC ATCAGTGCAAGCCTCACCTCTGTGGTGGACCAGGATGGTGGAGTCCATGTGCTTCAGGTACCTGTGTGGA ACCAGCAAGAAGTAGACATTCATATACCCATCACACTCTATCCCAGCATCCTGACGTTTCCATGGCAACC AAAGACAGGAGCCTATCAATATACTATAAAGGCCCATGGGGGCAGTGGGAACTTCACCTGGTCTTCCTCG AGCTACATGGTTGCCACGGTTACTGTCAAGGGTGTCATGACCACAGGCGGTGACACTGGCCTCAGTGTGA TCCGGGCACATGATGTTCAGAACCCTCTCCATTTTGGTGAGATGAAGGTGTATGTGATTGAGCCCAGCAG CATGGAGTTTGCCCCGTGCCAGGTGGAGGCTCGAGTGGGTCACACCCTGGAGCTGCCCCTGACAATCAGT GGCCTCATGCCCGGTGGGAGCAGCGAGGTGGTCACCCTGAGCGACTGCTCCCATTTTGACCTGGTGGTGG AGGTGGAGAACCAGGGTGTGTTCCAGCCATTGCCAGGGAGGCTGCCACCAGGGCCTGAGCACTGCAGTGG AGTCAAAGTGAGAGCTGATGCCCAGGGGTCCACTACCCTCCTTGTCAGCTACACACATGGCCATGTGCAC TTGGGTGCAAAGATCACTCTTGCTGCCTACCTGCCCCTCAAGGCTGTGGATCCCTCTTCTGTTGCTGTGG TAACCCTGGGATCCTCAAAGGAGATGCTGTTTGAAGGAGGCCCCAGGCCATGGGTCCTGGAACCTTCCAA ATTCTTTCGGAATGTCACTTCTGAAGACACAGGCAGCATCAGTCTCTCTCTCTTGGGTCCTCCAGCCTCT CGGAATTACCAGCAGCACCGGGTCCTTGTGACCTGCCAGGCCCTGGGTGAGCAGGTCATCGCCCTGTCTG TGGGGAACCGGCCCAGCCTCTCTAACCCCTTCCCTGCTGTGGAGCCCACTGTGGTGAAATCCGTCTGCGC CCCACCGTCCAGGCTTACTCTCATGCCTGTCTATGCCCTCCCGCAGCTCGACCTCTCTTGTCCATTGCTG CAGCAGAATAAGCAGGTGGTTCCAGTATCCAGTCACCGCAATCCCCTGTTAGACCTGGGTGCTTATGACC AACAAGGCCGGCGATTCGATAACTTCAGCTCTCTGAGCATCCAGTGGGAGTCCTTTCGGCCACTGCTGGC CAGCATTGAGGTTGACCAGCCCATGCAGCTGGTATCCCAGGATGATGGGAATGGCCAAAAGAAACTACAT GGTTTGCAGACAGTTTCGGTTCACGAGGCATCAGGAACCACAGCCATCTCTGCCACGGCGACTGGCTACC AGCAGTCCCACCTGAGTGCAGCCGGAGTGAAGCAGCTGCGTGACCCTCTAGTACCTGTGTCAGCCTCCAT TGAGCTTATACTCGTAGAGGATGTGAGGGTCAGTCCAGAAGAAGTGACCATCTATAACCATCCTGGTGTC CAGGTAGAACTCCACATCACAGAAGGCTCTGGCTATTTCTTCCTCAACACAAGCACCCAGGACATCATCA ATGTGGCCTACCAGGACACCCGGGGCGTGGCTATGGTGCATCCTCTGTTCCCAGGCTCATCAACTGTCAT GGTCCATGACTTGTGCCTGACCTTCCCAGCCCCAGCAAAGGCCACCATTCATGTCTCCGACATCCAGGAG CTCTATGTCCGAGTGGTGGACAAGGTGGAGATCGGGAAGGCTGTCAAGGCCTATGTCCGTGTGTTGGACT TCTACAAGAAGCCTTTCCTGGCCAAGTACTTCACCTTTATGGACCTGAAGCTGCGAGCAGCCTCCCAGAT CATCACGCTGGTGACTCTGGATGAAGCTCTAGACAATTACACGGCCACATTCCTTGTGCACGGCGTGGCC ATTGGCCAAACCAGCCTGTCGGCAAGTGTGACGGACAAATCTGGGCAGAGAGTCAGCTCCACCGCACAAC AGATCGAGGTCTTCCCCCCATTCAGACTGATACCAAGGAAGGTGACACTGATTATTGGAGCCATGATACA GATCACCTCCGAGGGCGGCCCCCAGCCTCAGTCCAACATCCTCTTCTCCATCAACAATGAGAGTGTGGCT GCAGTGAGCAGCGCCGGGCTGGTGCGTGGGCTCATGGTTGGCAATGGCTCTGTACTGGGAGTTGTGCAGG CAGTGGATGCCGAGACTGGCAAGGTCATCATCGTCTCCCAGGATCATGTGGAAGTAGAGGTGCTACAGCT CCAGGCAGTGAGGATCCGAGCCCCGATCACACGAATGAGGACCGGGACCCAGATGCCTGTCTATGTCACC GGGATCACCAGCAACCAGAGCCCTTTCTCCTTTGGCAATGCTGTGCCAGGCCTGACCTTCCACTGGTCTG TCACCAAGAGGGATGTCCTGGACCTTCGAGGACGGCACCATGAGGTGTCAATCCGGCTCTCGCCTCAGTA CAATTTTGCCATGAATGTGCATGGCCGGGTGAAAGGTCGGACTGGACTGCGGGTGGTGGTCAAAGCTCTG GACCCCACAGCTGGGCAGCTGCATGGTCTCGGCAAGGAGCTCTCAGATGAGATCCAAATTCAGGTATTTG AAAAACTGCGGCTGCTGAACCCTGAAGTAGAAGCAGAACAAATACTAATGTCACCTAACTCGTTTATAAA GCTGCAGACAAACAGGGATGGTGCAGCCATTCTGAGCTACCGGGTCCTCGATGGGCCTGAGAAGGCTCCC ATCGTGCACATTGACGAGAAGGGCTTTCTGGTGTCCGGCTCTGGGATTGGAGTGTCCACCCTCGAAGTGA TTGCACAGGAGCCTTTCGGCACCAACCAGACTGTCCTTGTCGCTGTAAAGGTCTCTCCTATTTCATACCT GAGGATCTCCATGAGCCCAGTCCTGCACACTCAGCACAAGGAGGTGCTGACAGCTCTGCCTCTGGGGATG ACTGTGACCTTCACTGTCCACTTCCATGACAGCTCTGGAGACATCTTCCACGCTCATAATTCAGACCTCA ATTTCGCTACTAACAGAGATGACTTCGTGCAGATTGGCAAGGGGGCCACCAACAACACCTGCATCATCCG CACAGTCAGTGTGGGCTTGACACTGCTCCACGTGTGGGATGTGGAACATCTCGGCCTCTCCGACTTCGTG CCACTACCTGTCCTGCAGGCCATCACCCCAGAGTTGTCTGGAGCTGTGGTGGTGGGCGACATCCTCTGCC TGGCTAGCGTTCTCATTAGCCTGGGAGGTGTCTCAGGGACCTGGAGCTCCTCAGCTGGCAATGTCCTCTA TGTTGACCCCAAGACAGGCGTGGCTATAGCCCGAGATGCAGGGCCTGTGACTGTTTACTATGAAATCGCT GGACATCTGAAGACCTTCAAGGAGATAGTGGTCGTCACCCCTCAGAAGATTGTGGCCCGTCGCCTCCACG CCACGCAGACCAGTATCCAGGAAGCCACAGCCTCTAAAGTGACTGTCAGCGTGGGAGACAGGAGCTCTAA CCTACTTGGTGAGTGCTCCTCTGCCCAGCGAGAGGCCATAGAAGCCTTGCACCCAGAGTCCCTCATCAGC TGCCAACTCCAGTTCAAGCAAGATGTCTTTGACTTCCCTGCTCGTGACATCTTCTCTGTGGAACCAGGAT TTGATACTGCACTGGGCCAGTACCTCTGCTCGGTTACTATGCACAGACTGACAGATAAGCAACTGAAGCA CTTGAACATGAAGAAGACATCACTGGCGGTCACCGCCTCCATGCCTAGCAGCCGTACATCTGTAGAAAAG GTGGGAGCTGAGGTCCCCTTCAGTCCAGGGCTCTATGCCAACCAGGCAGAAATCCTTTTGAGCAACCACT ACCCCAGCTCCGAGGTCAAGATCTTCGGTGCTGTGGAAATTCTGGAAAACTTGGAGGTGAGGTCCGGGTC CCCAGCAGTGCTAGCCTCCGTGAAAGAGAAGTCATTTGGACTGCCCAGCTTCATCACGTACACAGTTGGT GTCTTGGATCCCACAGCTGGCAGCCAAGGCCCTCTGTCCACTGCCCTGACCTTCTCCAGTCCTGCCACTA ACCAGGCCATCACCATTCCAGTCACTGTGGCCTTTGTGTTGGATCGTCGTGGGCCTGGTCCCTATGGAGC CAGCCTCCTGTCACACTTCCTGGACTCCTATCAAGTCATGTTCTTCACCTTTTTTGCCCTGCTGGCTGGG ACAGCAGTCACCATTATAGCCTACCACACGGTCTGTGCACCCCGGGAACTTGCTTCACCACTGGCCCTCA CGCCCCATGCCAGCCCTCAGCACAGCCCCCACTATCTTGCCTCATCCCCGACTGCTTTCAACACACTGCC TTCTGATCGCAAAGCCAGCCCTCCCTCAGGGCTCTGGAGCCCAGCCTATGCCTCTCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053322
Insert Size	5661 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053322.2, NP_445774.1
RefSeq Size	6941 bp
RefSeq ORF	5661 bp
Locus ID	58958
UniProt ID	P11654
Gene Summary	major component of the nuclear pore complex; may play a role in anchoring the central framework of the pore complex to the membrane [RGD, Feb 2006]

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