Zw10 (NM_001024801) Rat Untagged Clone
CAT＃: RN207355
Zw10 (untagged ORF) - Rat ZW10, kinetochore associated, homolog (Drosophila) (Zw10), (10 ug)
CNY 9980.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207355 representing NM_001024801
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTTTCGTGACGGAAGTCTTGGCCCACTCGGGGAGCCTGGAGAAGGAGGATCTGGGCACCAGGA TCAGCCGTCTGACCCGGCGGGTGGAGGAGATCAAGGGCGAGGTGTGCAATATGATCAGCAAGAAGTACAG TGAATTCCTGCCTACCATGCAGAGCGCACAGGCCCTCGTCACCCAGGTGGACACTCTATCCAATGACATC GACCAGCTGAAATCCAGGATAGAGACTGAGGTCTGTCGGGATCTTCACATATCAACTGTTGAGTTTACGA ACTTGAAGCAGCGGCTGGAAAGAGACTCTGTCGTCCTAAATTTGCTCAAACAGCTGCAAGAGTTTTCTTC TGCTATTGAAGAGTATAACAGTGCACTGGCAGAGAAGAAATACATCCCTGCTGCTCGGCTCCTGGAGGAG GCACAGGAATGCTTGAAGTTATTAAAATCAAAAAAATGCTTTGACTTAAAAATGTTGAAGTCTCTCAGCA TGGAGCTCACGGTACAGAAGCAGAACATCCTTTACCACCTGGGAGAAGACTGGCAGAAGCTGGTTGTATG GAAGTTCCCGCCATCAAAAGATACCAGCAGCTTAGAATCTTGTCTACAAACAGAACTTCATTTATGCACT GAGCAGCCCGAGAAGGAGATGACTCCTCTGCCATCCATCAGCTCTGTCCTCTTGGCGTTTTCTATTCTTG GAGAATTACCCACCAAGCTGAAGTCATTTGGTCAGATGCTGCTGAAGTATATACTTAAGCCTCTGGTGAC TTGCCCGTCGCTCCATGCTGTGATTGAAAGGCAGCCGAATTCAGTTAGCATTTGTTTCCAGTCTCTGGCG ACGGACTCAGAACATCCACCACCACCCGAAGCTTTTGCAAAGATCCAGTTGGTATTGGAAGTGCTCCAGA AACAGCTTCTAGATTTGCCTCTTGATGCTGACCTAGAAATTGGAAAAGTCCCTGAGATCGTGTTGGCTGA GATGCTTGGTGAGGTGATCTGGGAGGACCTGTCCGATTGCCTCATCAGAAACTGCTTGGTTTACTCTATT CCCACCAATAGCAGCAAGCTACAGCAATATGAGGAGATCATACAGTCCACAGAGGAATTTGAAAAGTCCC TAAAAGAGATGAGATTTTTAAAAGGAGATACTACTGACTTGCTGAAATATGCTCGCAATATCAATTCTCA TTTTGCTAATAAGAAGTGCCAGGATGTGATTGTGGCGGCTAGACATCTAATGACCTCCGAAATTCACAAC ACCGTGAAGATTGGTCCTGATTGTGAGGAAACCCTACCAGACTTGCCCAGTCCTGATGCCGACCACAGGC TACAAGTGCAGGTGTGCAAAGTGCAGTTCACCGATGCCGGAAACTTAGAGCCCGAGACTTCGCTGGACCC TCGGTCCTTCTCTTTACCCACGTGCCGCATCAGTGAGGCTGTGAAGAAGTTAATGGAGCTCGCTTATCAG ACTTTACTTGAGGCAACAACAAGTAGCGACCAATGTGCTGTCCAGCTTTTTTACTCAGTGAGAAATATCT TCCATTTGTTCCATGATGTGGTGCCCACATACCACAAGGAGAACCTTCAGAAGCTTCCCCAGTTGGCTGC CATTCACCATAACAACTGCATGTATATTGCTCACCACTTGCTGACCCTCGGCCATCAGTTCAGATCGCGG CTCACCCCCATTCTTTGTGATGGCACGACCACTTTTGTGGACCTTGTACCTGGCTTCAGAAGACTTGGGA CGGAGTGTTTTTTGGCCCAGATGCGGACCCAGAAAGGAGAACTTCTGGAGAGATTATCAAGTGCTAGAAG CTTTGCAAACATGGATGATGAAGAGAATTATTCTGCAGCGAGCAAGGCAGTGAGGCAGGTGCTGCACCAG CTGAAGAGACTTGGGATCGTGTGGCAGGATGTCCTGCCGGTGAACATATACTGCAAGGCTATGGGGACCT TGCTGAACACCGTGATTGCTGAGATGATTGGCAGGATCACTGCTCTAGAGGACATCTCGACTGAAGATGG CGATAGATTGTATTCCCTGTGCAAAACAGTGATGGATGAAGGGCCACAAGTATTTGCACCTCTGTCTGAT GAAAACAAAAACAAGAAATACCAAGAAGAGGTTCCGGTCTATGTGTCCAAGTGGATGCCTTTCAAAGAAC TCATGATAATGCTACAAGCTAGCTTGCAGGAAATCGGAGATCGGTGGGCAGATGGAAAAGGGCCCCTGGC AACTGCATTCCCTTCCAGCGAAGTAAAAGCTTTAATTCGTGCCTTGTTCCAGAACACAGAAAGAAGAGCG GCTGCCCTTGCTAAGATTAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024801 |
| Insert Size | 2334 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024801.1, NP_001019972.1 |
| RefSeq Size | 2454 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 363059 |
| UniProt ID | Q4V8C2 |
| Gene Summary | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207355 | Zw10 (Myc-DDK-tagged ORF) - Rat ZW10, kinetochore associated, homolog (Drosophila) (Zw10), (10 ug) |
CNY 9980.00 |
|
| RR207355L3 | Lenti ORF clone of Zw10 (Myc-DDK-tagged ORF) - Rat ZW10, kinetochore associated, homolog (Drosophila) (Zw10), (10 ug) |
CNY 11780.00 |
|
| RR207355L4 | Lenti ORF clone of Zw10 (mGFP-tagged ORF) - Rat ZW10, kinetochore associated, homolog (Drosophila) (Zw10), (10 ug) |
CNY 12920.00 |
