Etf1 (NM_001008344) Rat Untagged Clone
CAT＃: RN207315
Etf1 (untagged ORF) - Rat eukaryotic translation termination factor 1 (Etf1), (10 ug)
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC94734 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207315 representing NM_001008344
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGACCCCAGTGCTGCCGATAGGAACGTGGAGATCTGGAAGATCAAGAAGCTCATTAAGAGCT TGGAGGCGGCCCGCGGCAATGGCACGAGCATGATATCACTGATCATTCCTCCCAAAGACCAGATTTCACG AGTGGCAAAAATGTTAGCAGATGAGTTTGGAACTGCGTCTAACATTAAGTCACGAGTAAACCGCCTTTCA GTCCTGGGAGCCATCACATCTGTACAACAGAGACTCAAACTTTATAACAAAGTACCTCCAAATGGTCTGG TTGTTTACTGTGGAACAATTGTAACAGAAGAAGGAAAGGAAAAGAAAGTTAACATTGACTTTGAACCTTT CAAACCAATTAACACATCATTGTATTTGTGCGACAACAAGTTTCATACAGAGGCTCTTACAGCGTTACTC TCAGATGACAGCAAGTTTGGTTTTATTGTAATAGACGGCAGTGGTGCTCTTTTTGGCACGCTCCAAGGAA ACACCAGAGAAGTCCTGCACAAATTCACTGTGGATCTCCCAAAGAAACACGGCAGAGGAGGTCAATCAGC CTTGCGTTTTGCCCGTTTAAGAATGGAAAAGCGACATAACTATGTTCGGAAAGTAGCAGAGACTGCTGTG CAGCTGTTTATTTCTGGAGACAAAGTGAATGTGGCTGGTTTGGTTTTAGCCGGATCAGCTGACTTTAAAA CTGAACTAAGTCAATCTGACATGTTTGACCAGAGGTTGCAATCTAAAGTTTTAAAATTAGTTGATATATC CTACGGTGGTGAAAATGGATTCAACCAAGCTATTGAATTGTCTACTGAAGTTCTCTCCAACGTGAAGTTC ATTCAAGAGAAGAAATTAATAGGACGATACTTTGATGAAATTAGCCAGGACACGGGCAAGTACTGTTTTG GAGTTGAAGACACACTGAAGGCTTTGGAAATGGGAGCTGTAGAGATTCTAATAGTCTATGAAAATCTGGA TATAATGAGATACGTGCTTCATTGCCAAGGCACAGAAGAGGAGAAAATTCTCTATTTAACGCCAGAACAA GAGAAGGATAAATCTCACTTCACAGACAAAGAGACTGGACAGGAACATGAGCTGATAGAGAGCATGCCCC TCTTGGAATGGTTTGCTAACAACTATAAAAAATTTGGAGCTACACTAGAAATTGTCACAGATAAGTCACA AGAAGGATCACAGTTTGTGAAAGGATTTGGTGGAATTGGAGGTATCTTGCGGTACCGAGTAGATTTCCAG GGAATGGAATACCAAGGAGGAGATGACGAATTTTTTGACCTTGATGACTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001008344 |
| Insert Size | 1314 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008344.1, NP_001008345.1 |
| RefSeq Size | 2293 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 307503 |
| UniProt ID | Q5U2Q7 |
| Gene Summary | Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (By similarity). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207315 | Etf1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation termination factor 1 (Etf1), (10 ug) |
CNY 5610.00 |
|
| RR207315L3 | Lenti ORF clone of Etf1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation termination factor 1 (Etf1), (10 ug) |
CNY 7510.00 |
|
| RR207315L4 | Lenti ORF clone of Etf1 (mGFP-tagged ORF) - Rat eukaryotic translation termination factor 1 (Etf1), (10 ug) |
CNY 8360.00 |
